LOCUS       sequence-421880-        7285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV construct expressing HA-hM3Dq-dTomato driven by SST 
            interneuron-targeting enhancer. Alias: pAAV_WDS0004_Gq_dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Furlanis E, Dai M, Leyva Garcia B, Tran T, Vergara J, Pereira A, 
            Gorissen BL, Wills S, Vlachos A, Hairston A, Dwivedi D, Du S, 
            McMahon J, Huang S, Morabito A, Vazquez A, Kim S, Lee AT, Chang EF, 
            Razzaq T, Qazi A, Vargish G, Yuan X, Caccavano A, Hunt S, 
            Chittajallu R, McLean N, Hewitt L, Paranzino E, Rice H, Cummins AC, 
            Plotnikova A, Mohanty A, Tangen AC, Shin JH, Azadi R, Eldridge MAG, 
            Alvarez VA, Averbeck BB, Alyahyay M, Vallejo TR, Soheib M, Vattino 
            LG, MacGregor CP, Chatain CP, Banks E, Olah VJ, Naskar S, Hill S, 
            Liebergall S, Badiani R, Hyde L, Hanley E, Xu Q, Allaway KC, 
            Goldberg EM, Rowan MJM, Nowakowski TJ, Lee S, Favuzzi E, Kaeser PS, 
            Sjulson L, Batista-Brito R, Takesian AE, Ibrahim LA, Iqbal A, Pelkey
            KA, McBain CJ, Dimidschstein J, Fishell G, Wang Y
  TITLE     An enhancer-AAV toolbox to target and manipulate distinct 
            interneuron subtypes.
  JOURNAL   Neuron. 2025 May 21;113(10):1525-1547.e15. doi: 
            10.1016/j.neuron.2025.05.002.
  PUBMED    40403705
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.05"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1525-1547.e15"
COMMENT     Sequence Label: pAAV_BiSSTe4_Gq_dTomato
FEATURES             Location/Qualifiers
     source          1..7285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     intron          958..1090
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             1113..1139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2928..2984
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2997..3017
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3018..3716
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(3148..3166)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3416..3435
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3610..3633
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3771..4359
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3824..3844)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4242..4253)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4388..4469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4425..4444)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4479..4498
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4548..4688
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4548..4677
                     /label=AAV2 ITR
     rep_origin      4763..5218
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4850..4869)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5060..5081
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5235..5254)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5354..5376
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5500..5604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5605..5673,5674..6465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5823..5842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6636..7224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7125..7144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcgat tttcagcata agcaagatgg aatggacaat
      181 ctcttatttc atcctaagtt ttacaggagg tccttgtttt atgtaaattt ccaaccattt
      241 caaaagctct ttatgtgtgt attttttttt taaccatttt tgacagtttc aactctttct
      301 tctcccatga tttctttttg ctctcaactg ttatgggaga gacaccaggt tctgtatgca
      361 tttctgcttt gggttgaaaa ttttatgcac ttcaaatcaa gcttcattca catagctaaa
      421 ttcactgagg atacttcttc cctttgaaaa tttgacaaga ttcttaacaa cccttccccg
      481 ttctcctcat atttgttgag tttcctagtg catgtccttc tccctgcccc cagaataact
      541 agcaggtgtg acacaggcat gcattttgca ttttccaagt ctgtgatgct aactttaggc
      601 ctagaaacat tagcagattt cctcttttta tcatgcatac atttgctgct tctgttatcc
      661 aatctagtca ctttaaagaa ttttcttatt tcttttggga cctgtgcaat ctgttgcaag
      721 ctagaaatgt ccatggctag accaataaat tcactagata aatttacctg tggccacatt
      781 atttaaaagt ctgtgatcga tgtttaaact tcctgcagcc cgggctgggc ataaaagtca
      841 gggcagagcc atctattgct tacatttgct tctagcctgc aagtcgagga gcgcagcctt
      901 ccagaagcag agcgcggcgc cttaagctgc agaagttggt cgtgaggcac tgggcacgta
      961 agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag
     1021 agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt
     1081 ctctccacag cgagctcgct agcgccacca tgtacccata cgatgttcca gattacgcta
     1141 ccttgcacaa taacagtaca acctcgcctt tgtttccaaa catcagctcc tcctggatac
     1201 acagcccctc cgatgcaggg ctgcccccgg gaaccgtcac tcatttcggc agctacaatg
     1261 tttctcgagc agctggcaat ttctcctctc cagacggtac caccgatgac cctctgggag
     1321 gtcataccgt ctggcaagtg gtcttcatcg ctttcttaac gggcatcctg gccttggtga
     1381 ccatcatcgg caacatcctg gtaattgtgt catttaaggt caacaagcag ctgaagacgg
     1441 tcaacaacta cttcctctta agcctggcct gtgccgatct gattatcggg gtcatttcaa
     1501 tgaatctgtt tacgacctac atcatcatga atcgatgggc cttagggaac ttggcctgtg
     1561 acctctggct tgccattgac tgcgtagcca gcaatgcctc tgttatgaat cttctggtca
     1621 tcagctttga cagatacttt tccatcacga ggccgctcac gtaccgagcc aaacgaacaa
     1681 caaagagagc cggtgtgatg atcggtctgg cttgggtcat ctcctttgtc ctttgggctc
     1741 ctgccatctt gttctggcaa tactttgttg gaaagagaac tgtgcctccg ggagagtgct
     1801 tcattcagtt cctcagtgag cccaccatta cttttggcac agccatcgct ggtttttata
     1861 tgcctgtcac cattatgact attttatact ggaggatcta taaggaaact gaaaagcgta
     1921 ccaaagagct tgctggcctg caagcctctg ggacagaggc agagacagaa aactttgtcc
     1981 accccacggg cagttctcga agctgcagca gttacgaact tcaacagcaa agcatgaaac
     2041 gctccaacag gaggaagtat ggccgctgcc acttctggtt cacaaccaag agctggaaac
     2101 ccagctccga gcagatggac caagaccaca gcagcagtga cagttggaac aacaatgatg
     2161 ctgctgcctc cctggagaac tccgcctcct ccgacgagga ggacattggc tccgagacga
     2221 gagccatcta ctccatcgtg ctcaagcttc cgggtcacag caccatcctc aactccacca
     2281 agttaccctc atcggacaac ctgcaggtgc ctgaggagga gctggggatg gtggacttgg
     2341 agaggaaagc cgacaagctg caggcccaga agagcgtgga cgatggaggc agttttccaa
     2401 aaagcttctc caagcttccc atccagctag agtcagccgt ggacacagct aagacttctg
     2461 acgtcaactc ctcagtgggt aagagcacgg ccactctacc tctgtccttc aaggaagcca
     2521 ctctggccaa gaggtttgct ctgaagacca gaagtcagat cactaagcgg aaaaggatgt
     2581 ccctggtcaa ggagaagaaa gcggcccaga ccctcagtgc gatcttgctt gccttcatca
     2641 tcacttggac cccatacaac atcatggttc tggtgaacac cttttgtgac agctgcatac
     2701 ccaaaacctt ttggaatctg ggctactggc tgtgctacat caacagcacc gtgaaccccg
     2761 tgtgctatgc tctgtgcaac aaaacattca gaaccacttt caagatgctg ctgctgtgcc
     2821 agtgtgacaa aaaaaagagg cgcaagcagc agtaccagca gagacagtcg gtcatttttc
     2881 acaagcgcgc acccgagcag gccttgtccg gagataacgg aagcggagcc actaacttct
     2941 ccctgttgaa acaagcaggg gatgtcgaag agaatcccgg gccatctaga atggccccca
     3001 agaagaagag gaaggtggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca
     3061 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     3121 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     3181 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     3241 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     3301 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     3361 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     3421 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     3481 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     3541 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     3601 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     3661 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaag
     3721 tcgacggcgc gccccacccc tgcagggaat tcgatatcaa gcttatcgat aatcaacctc
     3781 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3841 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3901 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3961 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4021 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4081 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4141 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg
     4201 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4261 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4321 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg
     4381 agagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4441 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4501 tgtatcagct tatcgatacc gcatgcacgt gcggaccgag cggccgcagg aacccctagt
     4561 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     4621 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     4681 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     4741 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4801 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
     4861 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4921 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     4981 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5041 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
     5101 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
     5161 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     5221 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     5281 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5341 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5401 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5461 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5521 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5581 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5641 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5701 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5761 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5821 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5881 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5941 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6001 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6061 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6121 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6181 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6241 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6301 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6361 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6421 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6481 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6541 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6601 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6661 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6721 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6781 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6841 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6901 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6961 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7021 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7081 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7141 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7201 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7261 ttttgctggc cttttgctca catgt
//
