LOCUS       sequence-421882-       10438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for genome editing by CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Camara E, Lenitz I, Nygard Y
  TITLE     A CRISPR activation and interference toolkit for industrial 
            Saccharomyces cerevisiae strain KE6-12.
  JOURNAL   Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w.
  PUBMED    32884066
REFERENCE   2  (bases 1 to 10438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-09-3"; volume: "10(1):14605. doi"; pages: " 
            10.1038/s41598-020-71648-w"
COMMENT     Sequence Label: pYN2_1
FEATURES             Location/Qualifiers
     source          1..10438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1752..1772
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1776..1796
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     gene            2127..3482
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     CDS             2471..3280
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2538..2557)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      3285..3482
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    3487..3990
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(join(4101..4892,4893..4961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4724..4743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4962..5066)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5169..5757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5658..5677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             6304..6315
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6322..7035
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      7047..7118
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7134..7161
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        7175..7250
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(8074..10438,1..1739)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(8074..10438,1..1739)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 aggagctcgg gtcacagatc cttaaagagc acccggtgga aaacacccag cttcagaatg
       61 agaagctcta tctgtactac cttcaaaatg gacgcgatat gtatgtggac caagagcttg
      121 atatcaacag gctctcagac tacgacgtgg accatatcgt ccctcagagc ttcctcaaag
      181 acgactcaat tgacaataag gtgctgactc gctcagacaa gaaccgggga aagtcagata
      241 acgtgccctc agaggaagtc gtgaaaaaga tgaagaacta ttggcgccag cttctgaacg
      301 caaagctgat cactcagcgg aagttcgaca atctcactaa ggctgagagg ggcggactga
      361 gcgaactgga caaagcagga ttcattaaac ggcaacttgt ggagactcgg cagattacta
      421 aacatgtcgc ccaaatcctt gactcacgca tgaataccaa gtacgacgaa aacgacaaac
      481 ttatccgcga ggtgaaggtg attaccctga agtccaagct ggtcagcgat ttcagaaagg
      541 actttcaatt ctacaaagtg cgggagatca ataactatca tcatgctcat gacgcatatc
      601 tgaatgccgt ggtgggaacc gccctgatca agaagtaccc aaagctggaa agcgagttcg
      661 tgtacggaga ctacaaggtc tacgacgtgc gcaagatgat tgccaaatct gagcaggaga
      721 tcggaaaggc caccgcaaag tacttcttct acagcaacat catgaatttc ttcaagaccg
      781 aaatcaccct tgcaaacggt gagatccgga agaggccgct catcgagact aatggggaga
      841 ctggcgaaat cgtgtgggac aagggcagag atttcgctac cgtgcgcaaa gtgctttcta
      901 tgcctcaagt gaacatcgtg aagaaaaccg aggtgcaaac cggaggcttt tctaaggaat
      961 caatcctccc caagcgcaac tccgacaagc tcattgcaag gaagaaggat tgggacccta
     1021 agaagtacgg cggattcgat tcaccaactg tggcttattc tgtcctggtc gtggctaagg
     1081 tggaaaaagg aaagtctaag aagctcaaga gcgtgaagga actgctgggt atcaccatta
     1141 tggagcgcag ctccttcgag aagaacccaa ttgactttct cgaagccaaa ggttacaagg
     1201 aagtcaagaa ggaccttatc atcaagctcc caaagtatag cctgttcgaa ctggagaatg
     1261 ggcggaagcg gatgctcgcc tccgctggcg aacttcagaa gggtaatgag ctggctctcc
     1321 cctccaagta cgtgaatttc ctctaccttg caagccatta cgagaagctg aaggggagcc
     1381 ccgaggacaa cgagcaaaag caactgtttg tggagcagca taagcattat ctggacgaga
     1441 tcattgagca gatttccgag ttttctaaac gcgtcattct cgctgatgcc aacctcgata
     1501 aagtccttag cgcatacaat aagcacagag acaaaccaat tcgggagcag gctgagaata
     1561 tcatccacct gttcaccctc accaatcttg gtgcccctgc cgcattcaag tacttcgaca
     1621 ccaccatcga ccggaaacgc tatacctcca ccaaagaagt gctggacgcc accctcatcc
     1681 accagagcat caccggactt tacgaaactc ggattgacct ctcacagctc ggaggggatg
     1741 agggagctga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtataag
     1801 gatcctaact cgagattgaa ttgaattgaa atcgatagat caattttttt cttttctctt
     1861 tccccatcct ttacgctaaa ataatagttt attttatttt ttgaatattt tttatttata
     1921 tacgtatata tagactatta tttatctttt aatgattatt aagattttta ttaaaaaaaa
     1981 attcgctcct cttttaatgc ctttatgcag tttttttttc ccattcgata tttctatgtg
     2041 ctgagcagtt acagagatgt tacgaaccac tagtgcactg cagtacagtt tagcttgcct
     2101 cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc
     2161 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
     2221 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
     2281 acggctcctc gctccagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
     2341 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
     2401 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
     2461 ataaacaaaa atgggtaagg aaaagactca cgtttcgagg ccgcgattaa attccaacat
     2521 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     2581 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     2641 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     2701 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     2761 tgcgatcccc ggcaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     2821 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     2881 tccttttaac agcgatcgcg tatttcgtct ggctcaggcg caatcacgaa tgaataacgg
     2941 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     3001 gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt
     3061 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     3121 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     3181 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     3241 taaattgcag tttcatttga tgctcgatga gtttttctaa agtaactgac aataaaaaga
     3301 ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt
     3361 aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga
     3421 agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac
     3481 tggagtatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
     3541 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
     3601 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
     3661 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa aaccacacac gaaaatcctg
     3721 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
     3781 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
     3841 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
     3901 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
     3961 gaggcgcgtg taagttacag gcaagcgatc cgtcccgagc ggccgcgatt atcaaaaagg
     4021 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4081 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4141 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4201 gagggcttac catctggccc cagtgctgca atgataccgc gggacccacg ctcaccggct
     4261 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4321 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4381 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4441 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4501 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4561 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4621 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4681 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4741 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4801 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4861 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4921 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4981 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5041 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc
     5101 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5161 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5221 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5281 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5341 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5401 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5461 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5521 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5581 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5641 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5701 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5761 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5821 tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg ccgcccctga attcgcatct
     5881 agactgaatg tgcttcagta ttacattttt tgccttcaac gccttgattg ttctattttt
     5941 gctaataata aatctatttc atcggactaa aagtccatta gttgtaagcg gatttagctc
     6001 agttgggaga gcgccagact gaagaaaaac ttcggtcaag tcatctggag gtcctgtgtt
     6061 cgatccacag aattcgcaga tggccggcat ggtcccagcc tcctcgctgg cgccggctgg
     6121 gcaacacctt cgggtggcga atgggacttg agacggaaag tgaaacgtga tttcatgcgt
     6181 cattttgaac attttgtaaa tcttatttaa taatgtgtgc ggcaattcac atttaattta
     6241 tgaatgtttt cttaacatcg cggcaactca agaaacggca ggttcggatc ttagctacta
     6301 gagaaagagg agaaatacta gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct
     6361 attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt
     6421 gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg
     6481 ccggttcctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt
     6541 tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg
     6601 caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa
     6661 tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaggac
     6721 ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc
     6781 gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat
     6841 ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt
     6901 ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag
     6961 aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg
     7021 gatgaactgt acaaatgacc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     7081 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     7141 cttcgggtgg gcctttctgc gtttatacgt ctcagtttta gagctagaaa tagcaagtta
     7201 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttatt
     7261 ttttgtcact attgttatgt aaaatgccac ctctgacagt atggaacgca aacttctgtc
     7321 tagtggatac tgatgagacc aaaccagatg tcaacacagc tacaacggtg agtaaggaaa
     7381 gagtgaggaa ctatcgcata cctgcattta aagatgccga tttgggcgcg aatcctttat
     7441 tttggcttca ccctcatact attatcaggg ccagaaaaag gaagtgtttc cctccttctt
     7501 gaattgatgt taccctcata aagcacgtgg cctcttatcg agaaagaaat taccgtcgct
     7561 cgtgatttgt ttgcaaaaag aacaaaactg aaaaaaccca gacacgctcg acttcctgtc
     7621 atcctattga ttgcagcttc caatttcgtc acacaacaag gtcctagcga cggctcacag
     7681 gttttgtaac aagcaatcga aggttctgga atggcgggaa agggtttagt accacatgct
     7741 atgatgccca ctgtgatctc cagagcaaag ttcgttcgat cgtactgtta ctctctctct
     7801 ttcaaacaga attgtccgaa tcgtgtgaca acaacagcct gttctcacac actcttttct
     7861 tctaaccaag ggggtggttt agtttagtag aacctcgtga aacttacatt tacatatata
     7921 taaacttgca taaattggtc aatgcaagaa atacatattt ggtcttttct aattcgtagt
     7981 ttttcaagtt cttagatgct ttctttttct cttttttaca gatcatcaag gaagtaatta
     8041 tctacttttt acaacaaata taaaacaaga tctatggaca agaagtattc tatcggactg
     8101 gatatcggga ctaatagcgt cgggtgggcc gtgatcactg acgagtacaa ggtgccctct
     8161 aagaagttca aggtgctcgg gaacaccgac cggcattcca tcaagaaaaa tctgatcgga
     8221 gctctcctct ttgattcagg ggaaaccgct gaagcaaccc gcctcaagcg gactgctaga
     8281 cggcggtaca ccaggaggaa gaaccggatt tgttaccttc aagagatatt ctccaacgaa
     8341 atggcaaagg tcgacgacag cttcttccat aggctggaag aatcattcct cgtggaagag
     8401 gataagaagc atgaacggca tcccatcttc ggtaatatcg tcgacgaggt ggcctatcac
     8461 gagaaatacc caaccatcta ccatcttcgc aaaaagctgg tggactcaac cgacaaggca
     8521 gacctccggc ttatctacct ggccctggcc cacatgatca agttcagagg ccacttcctg
     8581 atcgagggcg acctcaatcc tgacaatagc gatgtggata aactgttcat ccagctggtg
     8641 cagacttaca accagctctt tgaagagaac cccatcaatg caagcggagt cgatgccaag
     8701 gccattctgt cagcccggct gtcaaagagc cgcagacttg agaatcttat cgctcagctg
     8761 ccgggtgaaa agaaaaatgg actgttcggg aacctgattg ctctttcact tgggctgact
     8821 cccaatttca agtctaattt cgacctggca gaggatgcca agctgcaact gtccaaggac
     8881 acctatgatg acgatctcga caacctcctg gcccagatcg gtgaccaata cgccgacctt
     8941 ttccttgctg ctaagaatct ttctgacgcc atcctgctgt ctgacattct ccgcgtgaac
     9001 actgaaatca ccaaggcccc tctttcagct tcaatgatta agcggtatga tgagcaccac
     9061 caggacctga ccctgcttaa ggcactcgtc cggcagcagc ttccggagaa gtacaaggaa
     9121 atcttctttg accagtcaaa gaatggatac gccggctaca tcgacggagg tgcctcccaa
     9181 gaggaatttt ataagtttat caaacctatc cttgagaaga tggacggcac cgaagagctc
     9241 ctcgtgaaac tgaatcggga ggatctgctg cggaagcagc gcactttcga caatgggagc
     9301 attccccacc agatccatct tggggagctt cacgccatcc ttcggcgcca agaggacttc
     9361 tacccctttc ttaaggacaa cagggagaag attgagaaaa ttctcacttt ccgcatcccc
     9421 tactacgtgg gacccctcgc cagaggaaat agccggtttg cttggatgac cagaaagtca
     9481 gaagaaacta tcactccctg gaacttcgaa gaggtggtgg acaagggagc cagcgctcag
     9541 tcattcatcg aacggatgac taacttcgat aagaacctcc ccaatgagaa ggtcctgccg
     9601 aaacattccc tgctctacga gtactttacc gtgtacaacg agctgaccaa ggtgaaatat
     9661 gtcaccgaag ggatgaggaa gcccgcattc ctgtcaggcg aacaaaagaa ggcaattgtg
     9721 gaccttctgt tcaagaccaa tagaaaggtg accgtgaagc agctgaagga ggactatttc
     9781 aagaaaattg aatgcttcga ctctgtggag attagcgggg tcgaagatcg gttcaacgca
     9841 agcctgggta cttaccatga tctgcttaag atcatcaagg acaaggattt tctggacaat
     9901 gaggagaacg aggacatcct tgaggacatt gtcctgactc tcactctgtt cgaggaccgg
     9961 gaaatgatcg aggagaggct taagacctac gcccatctgt tcgacgataa agtgatgaag
    10021 caacttaaac ggagaagata taccggatgg ggacgcctta gccgcaaact catcaacgga
    10081 atccgggaca aacagagcgg aaagaccatt cttgatttcc ttaagagcga cggattcgct
    10141 aatcgcaact tcatgcaact tatccatgat gattccctga cctttaagga ggacatccag
    10201 aaggcccaag tgtctggaca aggtgactca ctgcacgagc atatcgcaaa tctggctggt
    10261 tcacccgcta ttaagaaggg tattctccag accgtgaaag tcgtggacga gctggtcaag
    10321 gtgatgggtc gccataaacc agagaacatt gtcatcgaga tggccaggga aaaccagact
    10381 acccagaagg gacagaagaa cagcagggag cggatgaaaa gaattgagga agggatta
//
