LOCUS       sequence-421906-       13254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  activating MTOR mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 13254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pcDNA3-FLAG-MTOR-E1799K
FEATURES             Location/Qualifiers
     source          1..13254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     503..522
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    807..928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(844..863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     898..917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(977..993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(977..993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(990..1012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1001..1017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1025..1031,1032..1049,1050..1055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1070..1091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1208..1225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1379..1967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1459..1478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2138..2929,2930..2998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2761..2780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2999..3103)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3178..3197)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3369..3748
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3749..3952
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3903..3923
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3997..4016
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3997..4015
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4101..4110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6982..6991
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10191..10469
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             10203..10475
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     primer_bind     complement(11890..11906)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11890..11906)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(11941..11959)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11942..11959)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11979..11996)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11985..12209
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12255..12683
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12342..12361)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12552..12573
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12692..12712)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12697..13026
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12877..13012
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12939..12958
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             join(13093..13254,1..633)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13147..13166)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
       61 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
      121 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
      181 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
      241 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
      301 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
      361 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
      421 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
      481 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
      541 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
      601 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
      661 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
      721 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
      781 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
      841 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      901 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
      961 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1021 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1081 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1141 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1201 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1261 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1321 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1381 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1441 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1501 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1561 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1621 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1681 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1741 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1801 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1861 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1921 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1981 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2041 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2101 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2161 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2221 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2281 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2341 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2401 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2461 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2521 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2581 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2641 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2701 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2761 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2821 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2881 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2941 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3001 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3061 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3121 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     3181 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     3241 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     3301 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     3361 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     3421 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     3481 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3541 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3601 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3661 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3721 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3781 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     3841 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     3901 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
     3961 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
     4021 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattctgc
     4081 agatatccat cacactggcg gccgccatgg attataaaga tgatgatgat atgcttggaa
     4141 ccggacctgc cgccgccacc accgctgcca ccacatctag caatgtgagc gtcctgcagc
     4201 agtttgccag tggcctaaag agccggaatg aggaaaccag ggccaaagcc gccaaggagc
     4261 tccagcacta tgtcaccatg gaactccgag agatgagtca agaggagtct actcgcttct
     4321 atgaccaact gaaccatcac atttttgaat tggtttccag ctcagatgcc aatgagagga
     4381 aaggtggcat cttggccata gctagcctca taggagtgga aggtgggaat gccacccgaa
     4441 ttggcagatt tgccaactat cttcggaacc tcctcccctc caatgaccca gttgtcatgg
     4501 aaatggcatc caaggccatt ggccgtcttg ccatggcagg ggacactttt accgctgagt
     4561 acgtggaatt tgaggtgaag cgagccctgg aatggctggg tgctgaccgc aatgagggcc
     4621 ggagacatgc agctgtcctg gttctccgtg agctggccat cagcgtccct accttcttct
     4681 tccagcaagt gcaacccttc tttgacaaca tttttgtggc cgtgtgggac cccaaacagg
     4741 ccatccgtga gggagctgta gccgcccttc gtgcctgtct gattctcaca acccagcgtg
     4801 agccgaagga gatgcagaag cctcagtggt acaggcacac atttgaagaa gcagagaagg
     4861 gatttgatga gaccttggcc aaagagaagg gcatgaatcg ggatgatcgg atccatggag
     4921 ccttgttgat ccttaacgag ctggtccgaa tcagcagcat ggagggagag cgtctgagag
     4981 aagaaatgga agaaatcaca cagcagcagc tggtacacga caagtactgc aaagatctca
     5041 tgggcttcgg aacaaaacct cgtcacatta cccccttcac cagtttccag gctgtacagc
     5101 cccagcagtc aaatgccttg gtggggctgc tggggtacag ctctcaccaa ggcctcatgg
     5161 gatttgggac ctcccccagt ccagctaagt ccaccctggt ggagagccgg tgttgcagag
     5221 acttgatgga ggagaaattt gatcaggtgt gccagtgggt gctgaaatgc aggaatagca
     5281 agaactcgct gatccaaatg acaatcctta atttgttgcc ccgcttggct gcattccgac
     5341 cttctgcctt cacagatacc cagtatctcc aagataccat gaaccatgtc ctaagctgtg
     5401 tcaagaagga gaaggaacgt acagcggcct tccaagccct ggggctactt tctgtggctg
     5461 tgaggtctga gtttaaggtc tatttgcctc gcgtgctgga catcatccga gcggccctgc
     5521 ccccaaagga cttcgcccat aagaggcaga aggcaatgca ggtggacgcc acagtcttca
     5581 cttgcatcag catgctggct cgagcaatgg ggccaggcat ccagcaggat atcaaggagc
     5641 tgctggagcc catgctggca gtgggactaa gccctgccct cactgcagtg ctctacgacc
     5701 tgagccgtca gattccacag ctaaagaagg acattcaaga tgggctactg aaaatgctgt
     5761 ccctggtcct tatgcacaaa ccccttcgcc acccaggcat gcccaagggc ctggcccatc
     5821 agctggcctc tcctggcctc acgaccctcc ctgaggccag cgatgtgggc agcatcactc
     5881 ttgccctccg aacgcttggc agctttgaat ttgaaggcca ctctctgacc caatttgttc
     5941 gccactgtgc ggatcatttc ctgaacagtg agcacaagga gatccgcatg gaggctgccc
     6001 gcacctgctc ccgcctgctc acaccctcca tccacctcat cagtggccat gctcatgtgg
     6061 ttagccagac cgcagtgcaa gtggtggcag atgtgcttag caaactgctc gtagttggga
     6121 taacagatcc tgaccctgac attcgctact gtgtcttggc gtccctggac gagcgctttg
     6181 atgcacacct ggcccaggcg gagaacttgc aggccttgtt tgtggctctg aatgaccagg
     6241 tgtttgagat ccgggagctg gccatctgca ctgtgggccg actcagtagc atgaaccctg
     6301 cctttgtcat gcctttcctg cgcaagatgc tcatccagat tttgacagag ttggagcaca
     6361 gtgggattgg aagaatcaaa gagcagagtg cccgcatgct ggggcacctg gtctccaatg
     6421 ccccccgact catccgcccc tacatggagc ctattctgaa ggcattaatt ttgaaactga
     6481 aagatccaga ccctgatcca aacccaggtg tgatcaataa tgtcctggca acaataggag
     6541 aattggcaca ggttagtggc ctggaaatga ggaaatgggt tgatgaactt tttattatca
     6601 tcatggacat gctccaggat tcctctttgt tggccaaaag gcaggtggct ctgtggaccc
     6661 tgggacagtt ggtggccagc actggctatg tagtagagcc ctacaggaag taccctactt
     6721 tgcttgaggt gctactgaat tttctgaaga ctgagcagaa ccagggtaca cgcagagagg
     6781 ccatccgtgt gttagggctt ttaggggctt tggatcctta caagcacaaa gtgaacattg
     6841 gcatgataga ccagtcccgg gatgcctctg ctgtcagcct gtcagaatcc aagtcaagtc
     6901 aggattcctc tgactatagc actagtgaaa tgctggtcaa catgggaaac ttgcctctgg
     6961 atgagttcta cccagctgtg tccatggtgg ccctgatgcg gatcttccga gaccagtcac
     7021 tctctcatca tcacaccatg gttgtccagg ccatcacctt catcttcaag tccctgggac
     7081 tcaaatgtgt gcagttcctg ccccaggtca tgcccacgtt ccttaatgtc attcgagtct
     7141 gtgatggggc catccgggaa tttttgttcc agcagctggg aatgttggtg tcctttgtga
     7201 agagccacat cagaccttat atggatgaaa tagtcaccct catgagagaa ttctgggtca
     7261 tgaacacctc aattcagagc acgatcattc ttctcattga gcaaattgtg gtagctcttg
     7321 ggggtgaatt taagctctac ctgccccagc tgatcccaca catgctgcgt gtcttcatgc
     7381 atgacaacag cccaggccgc attgtctcta tcaagttact ggctgcaatc cagctgtttg
     7441 gcgccaacct ggatgactac ctgcatttac tgctgcctcc tattgttaag ttgtttgatg
     7501 cccctgaagc tccactgcca tctcgaaagg cagcgctaga gactgtggac cgcctgacgg
     7561 agtccctgga tttcactgac tatgcctccc ggatcattca ccctattgtt cgaacactgg
     7621 accagagccc agaactgcgc tccacagcca tggacacgct gtcttcactt gtttttcagc
     7681 tggggaagaa gtaccaaatt ttcattccaa tggtgaataa agttctggtg cgacaccgaa
     7741 tcaatcatca gcgctatgat gtgctcatct gcagaattgt caagggatac acacttgctg
     7801 atgaagagga ggatcctttg atttaccagc atcggatgct taggagtggc caaggggatg
     7861 cattggctag tggaccagtg gaaacaggac ccatgaagaa actgcacgtc agcaccatca
     7921 acctccaaaa ggcctggggc gctgccagga gggtctccaa agatgactgg ctggaatggc
     7981 tgagacggct gagcctggag ctgctgaagg actcatcatc gccctccctg cgctcctgct
     8041 gggccctggc acaggcctac aacccgatgg ccagggatct cttcaatgct gcatttgtgt
     8101 cctgctggtc tgaactgaat gaagatcaac aggatgagct catcagaagc atcgagttgg
     8161 ccctcacctc acaagacatc gctgaagtca cacagaccct cttaaacttg gctgaattca
     8221 tggaacacag tgacaagggc cccctgccac tgagagatga caatggcatt gttctgctgg
     8281 gtgagagagc tgccaagtgc cgagcatatg ccaaagcact acactacaaa gaactggagt
     8341 tccagaaagg ccccacccct gccattctag aatctctcat cagcattaat aataagctac
     8401 agcagccgga ggcagcggcc ggagtgttag aatatgccat gaaacacttt ggagagctgg
     8461 agatccaggc tacctggtat gagaaactgc acgagtggga ggatgccctt gtggcctatg
     8521 acaagaaaat ggacaccaac aaggacgacc cagagctgat gctgggccgc atgcgctgcc
     8581 tcgaggcctt gggggaatgg ggtcaactcc accagcagtg ctgtgaaaag tggaccctgg
     8641 ttaatgatga gacccaagcc aagatggccc ggatggctgc tgcagctgca tggggtttag
     8701 gtcagtggga cagcatggaa gaatacacct gtatgatccc tcgggacacc catgatgggg
     8761 cattttatag agctgtgctg gcactgcatc aggacctctt ctccttggca caacagtgca
     8821 ttgacaaggc cagggacctg ctggatgctg aattaactgc aatggcagga gagagttaca
     8881 gtcgggcata tggggccatg gtttcttgcc acatgctgtc cgagctggag gaggttatcc
     8941 agtacaaact tgtccccgag cgacgagaga tcatccgcca gatctggtgg gagagactgc
     9001 agggctgcca gcgtatcgta gaggactggc agaaaatcct tatggtgcgg tcccttgtgg
     9061 tcagccctca tgaagacatg agaacctggc tcaagtatgc aagcctgtgc ggcaagagtg
     9121 gcaggctggc tcttgctcat aaaactttag tgttgctcct gggagttgat ccgtctcggc
     9181 aacttgacca tcctctgcca acagttcacc ctcaggtgac ctatgcctac atgaaaaaca
     9241 tgtggaagag tgcccgcaag atcgatgcct tccagcacat gcagcatttt gtccagacca
     9301 tgcagcaaca ggcccagcat gccatcgcta ctgaggacca gcagcataag caggaactgc
     9361 acaagctcat ggcccgatgc ttcctgaaac ttggagagtg gcagctgaat ctacagggca
     9421 tcaatgagag cacaatcccc aaagtgctgc agtactacag cgccgccaca gagcacgacc
     9481 gcagctggta caaggcctgg catgcgtggg cagtgatgaa cttcaaagct gtgctacact
     9541 acaaacatca gaaccaagcc cgcgatgaga agaagaaact gcgtcatgcc agcggggcca
     9601 acatcaccaa cgccaccact gccgccacca cggccgccac tgccaccacc actgccagca
     9661 ccgagggcag caacagtgag agcgaggccg agagcaccga gaacagcccc accccatcgc
     9721 cgctgcagaa gaaggtcact gaggatctgt ccaaaaccct cctgatgtac acggtgcctg
     9781 ccgtccaggg cttcttccgt tccatctcct tgtcacgagg caacaacctc caggatacac
     9841 tcagagttct caccttatgg tttgattatg gtcactggcc agatgtcaat gaggccttag
     9901 tggagggggt gaaagccatc cagattgata cctggctaca ggttatacct cagctcattg
     9961 caagaattga tacgcccaga cccttggtgg gacgtctcat tcaccagctt ctcacagaca
    10021 ttggtcggta ccacccccag gccctcatct acccactgac agtggcttct aagtctacca
    10081 cgacagcccg gcacaatgca gccaacaaga ttctgaagaa catgtgtgag cacagcaaca
    10141 ccctggtcca gcaggccatg atggtgagcg aggagctgat ccgagtggcc atcctctggc
    10201 atgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg gaaaggaacg
    10261 tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg ggcccccaga
    10321 ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag gcccaagagt
    10381 ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cacccaagcc tgggacctct
    10441 attatcatgt gttccgacga atctcaaagc agctgcctca gctcacatcc ttagagctgc
    10501 aatatgtttc cccaaaactt ctgatgtgcc gggaccttga attggctgtg ccaggaacat
    10561 atgaccccaa ccagccaatc attcgcattc agtccatagc accgtctttg caagtcatca
    10621 catccaagca gaggccccgg aaattgacac ttatgggcag caacggacat gagtttgttt
    10681 tccttctaaa aggccatgaa gatctgcgcc aggatgagcg tgtgatgcag ctcttcggcc
    10741 tggttaacac ccttctggcc aatgacccaa catctcttcg gaaaaacctc agcatccaga
    10801 gatacgctgt catcccttta tcgaccaact cgggcctcat tggctgggtt ccccactgtg
    10861 acacactgca cgccctcatc cgggactaca gggagaagaa gaagatcctt ctcaacatcg
    10921 agcatcgcat catgttgcgg atggctccgg actatgacca cttgactctg atgcagaagg
    10981 tggaggtgtt tgagcatgcc gtcaataata cagctgggga cgacctggcc aagctgctgt
    11041 ggctgaaaag ccccagctcc gaggtgtggt ttgaccgaag aaccaattat acccgttctt
    11101 tagcggtcat gtcaatggtt gggtatattt taggcctggg agatagacac ccatccaacc
    11161 tgatgctgga ccgtctgagt gggaagatcc tgcacattga ctttggggac tgctttgagg
    11221 ttgctatgac ccgagagaag tttccagaga agattccatt tagactaaca agaatgttga
    11281 ccaatgctat ggaggttaca ggcctggatg gcaactacag aatcacatgc cacacagtga
    11341 tggaggtgct gcgagagcac aaggacagtg tcatggccgt gctggaagcc tttgtctatg
    11401 accccttgct gaactggagg ctgatggaca caaataccaa aggcaacaag cgatcccgaa
    11461 cgaggacgga ttcctactct gctggccagt cagtcgaaat tttggacggt gtggaacttg
    11521 gagagccagc ccataagaaa acggggacca cagtgccaga atctattcat tctttcattg
    11581 gagacggttt ggtgaaacca gaggccctaa ataagaaagc tatccagatt attaacaggg
    11641 ttcgagataa gctcactggt cgggacttct ctcatgatga cactttggat gttccaacgc
    11701 aagttgagct gctcatcaaa caagcgacat cccatgaaaa cctctgccag tgctatattg
    11761 gctggtgccc tttctggtaa ctggaggccc agatgtgccc atcacgtttt ttctgaggct
    11821 tttgtacttt agtaaatgct tccactaaac tgaaaccatg gtgagaaagt ttgactttgt
    11881 taaatggggg atccactagt tctagagcgg ccgctcgagc atgcatctag agggccctat
    11941 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
    12001 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
    12061 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
    12121 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
    12181 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
    12241 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
    12301 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
    12361 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
    12421 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
    12481 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
    12541 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
    12601 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
    12661 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
    12721 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    12781 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    12841 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    12901 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    12961 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    13021 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
    13081 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
    13141 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
    13201 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtcc
//
