LOCUS       sequence-421913-        5069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5069)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-H2A-10
FEATURES             Location/Qualifiers
     source          1..5069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             170..964
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             170..964
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(224..243)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     834..853
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1152..1171)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1196..1243
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1572..2160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2061..2080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2335..2638
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2639..2842
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2793..2813
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3292..3301
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3298..4008
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3443..3461)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3711..3730
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4131..4252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4168..4187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4222..4241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4259..4714)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4396..4417)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4608..4627
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4741..4845
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(4847..5069,1..135)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4847..5038
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4870..4890)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(5055..5069,1..121)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
       61 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      121 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
      181 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
      241 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
      301 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
      361 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
      421 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
      481 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
      541 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
      601 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
      661 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
      721 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
      781 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
      841 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
      901 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
      961 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     1021 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     1081 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     1141 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     1201 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     1261 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     1321 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     1381 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     1441 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1501 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1561 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1621 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1681 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1741 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1801 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1921 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1981 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2041 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2101 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2161 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2221 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
     2281 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     2341 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     2401 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     2461 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     2521 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     2581 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     2641 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     2701 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     2761 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     2821 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc cgccaccatg
     2881 tcgggacgtg gcaagcaggg cggcaaagct cgcgccaagg ccaagacccg ctcttctcgg
     2941 gccgggcttc agtttcccgt aggccgagtg catcgcctgc tccgcaaagg caactatgcg
     3001 gagcgggtcg gtgctggagc gccggtgtac ctggcggcgg tgctggagta cctgaccgcc
     3061 gagatcctgg agctggctgg caacgcggcc cgcgacaaca agaagactcg catcatcccg
     3121 cgtcacctcc agctggccat ccgcaacgat gaggagctca acaagcttct gggcaaagtc
     3181 accatcgcac agggtggcgt cctgcccaac atccaggccg tgctactgcc caagaagacc
     3241 gagagccacc acaaggcgaa gggcaagtcg ggaagcacgg atccaccggt cgccaccatg
     3301 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     3361 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     3421 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     3481 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     3541 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     3601 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     3661 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     3721 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     3781 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     3841 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     3901 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     3961 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaag cggccgcgac
     4021 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     4081 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     4141 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4201 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa
     4261 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     4321 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     4381 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     4441 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     4501 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     4561 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     4621 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     4681 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     4741 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4801 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     4861 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4921 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4981 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     5041 ccctaactcc gcccatcccg cccctaact
//
