LOCUS       sequence-421919-       13249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. MCS upstream of gateway, driven by UBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 13249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pUBQ10-GW
FEATURES             Location/Qualifiers
     source          1..13249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    2924..3048
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3073..3078,3079..3096,3097..3103)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3157..3816
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3223..3242)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4158..4463
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4342..4361
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4504..4628)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4681..5388
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5413..5430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5413..5429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5422..5444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5632..5656
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6957..7586
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8015..9088
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9154..9348
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9584..9606)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9692..9832
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9747..9766
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9847..9864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10018..10606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10098..10117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12031..12825)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gtatgatcgc gaagcaccca ccctaagcca cattttaatc cttctgttgg
     1381 ccatgcccca tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg
     1441 tctgtcccga cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa
     1501 tccactcatc tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc
     1561 ctttaagcac cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag
     1621 cctccacatt cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg
     1681 tatgaactga tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga
     1741 atgttttgtg tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg
     1801 actgaacacg agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt
     1861 aaataacaag aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac
     1921 caacttgata caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata
     1981 acactaaaaa attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac
     2041 ttttccaaga aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa
     2101 acaaaaagga aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg
     2161 cagtgggacc cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata
     2221 aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct
     2281 tatgacgacc gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg
     2341 ttgcagccgg cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac
     2401 ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg
     2461 tcattttatt attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg
     2521 tcttttcttc ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca
     2581 gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt
     2641 aaatttctgt gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt
     2701 tcgtatatgt tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt
     2761 tgatcgttag atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt
     2821 caaataattt gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg
     2881 gtgttagttt ctagtttgtg cgatcgaatt tgtcctcgag atcacaagtt tgtacaaaaa
     2941 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     3001 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
     3061 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     3121 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     3181 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     3241 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     3301 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     3361 gctcatccgg agttccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     3421 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     3481 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     3541 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     3601 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     3661 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     3721 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     3781 caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa
     3841 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     3901 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     3961 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     4021 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     4081 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     4141 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     4201 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     4261 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     4321 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     4381 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     4441 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     4501 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     4561 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     4621 aaagtggtgc ctaggtgagt ctagagagtt aattaagacc cgggactagt ccctagagtc
     4681 ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt
     4741 gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc
     4801 attctaatga atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa
     4861 tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca atatattgtg
     4921 ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca attgcgtttt
     4981 attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt
     5041 acataaatct tattcaaatt tcaaaagtgc cccaggggct agtatctacg acacaccgag
     5101 cggcgaacta ataacgctca ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga
     5161 gattccttga agttgagtat tggccgtccg ctctaccgaa agttacgggc accattcaac
     5221 ccggtccagc acggcggccg ggtaaccgac ttgctgcccc gagaattatg cagcattttt
     5281 ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt
     5341 tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg ctcagcagga cctgcaggca
     5401 tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     5461 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     5521 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt
     5581 gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat
     5641 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     5701 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     5761 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     5821 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     5881 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     5941 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     6001 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     6061 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     6121 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     6181 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     6241 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     6301 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     6361 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
     6421 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
     6481 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
     6541 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
     6601 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
     6661 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
     6721 atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa
     6781 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
     6841 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
     6901 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
     6961 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
     7021 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
     7081 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
     7141 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
     7201 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
     7261 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
     7321 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
     7381 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
     7441 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
     7501 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
     7561 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
     7621 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
     7681 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
     7741 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
     7801 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
     7861 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
     7921 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca
     7981 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
     8041 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
     8101 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     8161 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     8221 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     8281 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     8341 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
     8401 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
     8461 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
     8521 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
     8581 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
     8641 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
     8701 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
     8761 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
     8821 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
     8881 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
     8941 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
     9001 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
     9061 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
     9121 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc
     9181 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
     9241 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
     9301 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
     9361 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
     9421 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
     9481 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
     9541 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     9601 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     9661 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     9721 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     9781 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     9841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    10081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    10141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    10201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    10261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    10321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    10381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    10441 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    10501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    10561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    10621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaagggc tgatgaatcc
    10681 cctaatgatt ttggtaaaaa tcattaagtt aaggtggata cacatcttgt catatgatca
    10741 aatggtttcg cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac
    10801 gactctcttt accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg
    10861 gcttgccacg cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg
    10921 ccaaccaaag cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt
    10981 cacctccaca aagagcgact cgctgtatac cgttggcatg ctagctttat ctgttcgggc
    11041 aatacgatgc ccattgtact tgttgactgg tctgatattc gtgagcaaaa acgacttatg
    11101 gtattgcgag cttcagtcgc actacacggt cgttctgtta ctctttatga gaaagcgttc
    11161 ccgctttcag agcaatgttc aaagaaagct catgaccaat ttctagccga ccttgcgagc
    11221 attctaccga gtaacaccac accgctcatt gtcagtgatg ctggctttaa agtgccatgg
    11281 tataaatccg ttgagaagct gggttggtac tggttaagtc gagtaagagg aaaagtacaa
    11341 tatgcagacc taggagcgga aaactggaaa cctatcagca acttacatga tatgtcatct
    11401 agtcactcaa agactttagg ctataagagg ctgactaaaa gcaatccaat ctcatgccaa
    11461 attctattgt ataaatctcg ctctaaaggc cgaaaaaatc agcgctcgac acggactcat
    11521 tgtcaccacc cgtcacctaa aatctactca gcgtcggcaa aggagccatg ggttctagca
    11581 actaacttac ctgttgaaat tcgaacaccc aaacaacttg ttaatatcta ttcgaagcga
    11641 atgcagattg aagaaacctt ccgagacttg aaaagtcctg cctacggact aggcctacgc
    11701 catagccgaa cgagcagctc agagcgtttt gatatcatgc tgctaatcgc cctgatgctt
    11761 caactaacat gttggcttgc gggcgttcat gctcagaaac aaggttggga caagcacttc
    11821 caggctaaca cagtcagaaa tcgaaacgta ctctcaacag ttcgcttagg catggaagtt
    11881 ttgcggcatt ctggctacac aataacaagg gaagacttac tcgtggctgc aaccctacta
    11941 gctcaaaatt tattcacaca tggttacgct ttggggaaat tatgagggga tctctcagcg
    12001 ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata
    12061 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
    12121 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
    12181 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
    12241 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
    12301 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
    12361 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
    12421 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
    12481 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
    12541 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
    12601 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
    12661 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
    12721 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
    12781 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
    12841 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
    12901 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
    12961 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
    13021 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    13081 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    13141 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    13201 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctgg
//
