LOCUS       sequence-421938-        9352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Doxy Inducible Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Reilly CR, Myllymaki M, Redd R, Padmanaban S, Karunakaran D, Tesmer 
            V, Tsai FD, Gibson CJ, Rana HQ, Zhong L, Saber W, Spellman SR, Hu 
            ZH, Orr EH, Chen MM, De Vivo I, DeAngelo DJ, Cutler C, Antin JH, 
            Neuberg D, Garber JE, Nandakumar J, Agarwal S, Lindsley RC
  TITLE     The clinical and functional effects of TERT variants in 
            myelodysplastic syndrome.
  JOURNAL   Blood. 2021 Sep 9;138(10):898-911. doi: 10.1182/blood.2021011075.
  PUBMED    34019641
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1182/blood"; 
            journalName: "Blood"; date: "2021-09-9- 9"; volume: "138"; issue: 
            "10"; pages: "898-911"
COMMENT     Sequence Label: Luciferase
FEATURES             Location/Qualifiers
     source          1..9352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        73..144
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(145..213,214..1005)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(363..382)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1176..1764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1665..1684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1918..1935
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2052..2073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2088..2093,2094..2111,2112..2118)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2126..2142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2131..2153
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2150..2166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2150..2166
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2185..2205
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2187..2205
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2233..2459
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2460..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2687..2812
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3305..3538
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3723..3767
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3980..4097
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4151..4465
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4159..4177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4195..4213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4230..4248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4266..4284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4302..4320
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4337..4355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4373..4391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4438..4462
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4486..4510
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4513..6162
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4579..4597)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    complement(6164..6188)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6206..6223
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        6233..6743
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6662..6680
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6753..7349
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6753..6772)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7249..7269
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7350..7403
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7404..8021,8022..8027,8028..8063,8064..8066,8067..8102,
                     8103..8105,8106..8141,8142..8150)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8190..8210)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8608..8619)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8824..9057
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9129..9250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9166..9185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9220..9239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      join(9290..9352,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      121 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      181 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      241 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      301 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      361 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      421 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      481 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      541 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      601 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      661 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      721 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      781 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      841 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      901 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
      961 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1021 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1141 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1201 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1261 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1321 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1381 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1441 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1501 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1561 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1621 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1681 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1741 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1801 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1861 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1921 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1981 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2041 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     2101 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     2161 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     2221 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     2281 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     2341 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     2401 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     2461 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     2521 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     2581 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2641 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     2701 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     2761 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     2821 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     2881 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     2941 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     3001 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     3061 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     3121 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     3181 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     3241 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     3301 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     3361 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     3421 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     3481 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     3541 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     3601 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     3661 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     3721 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     3781 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     3841 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     3901 cattatcgtt tcagacccac ctcccaaccc cgaggggaca attctcgacc tcgagacaaa
     3961 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     4021 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     4081 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcgaa
     4141 tcgatatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     4201 tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     4261 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     4321 acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     4381 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
     4441 tcgtttagtg aaccgtcaga tcgcctggag aattggctag catcaacaag tttgtacaaa
     4501 aaagttggca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg
     4561 ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     4621 cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac
     4681 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     4741 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     4801 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     4861 agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt
     4921 ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg
     4981 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     5041 aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg
     5101 aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc
     5161 gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     5221 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     5281 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc
     5341 cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc
     5401 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc
     5461 gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc
     5521 aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     5581 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     5641 gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg
     5701 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     5761 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac
     5821 cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc
     5881 atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac
     5941 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     6001 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     6061 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     6121 gagatcctca taaaggccaa gaagggcgga aagatcgccg tgttgccaac tttcttgtac
     6181 aaagtggttt agtaatgaac cggtccacca ccaccaccac cactaaggat ccggggttgg
     6241 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     6301 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     6361 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     6421 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     6481 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     6541 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     6601 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     6661 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     6721 gaatcaccga cctctctccc cagcaattca ccatgaccga gtacaagccc acggtgcgcc
     6781 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6841 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6901 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6961 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     7021 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     7081 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     7141 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     7201 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     7261 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     7321 cctggtgcat gacccgcaag cccggtgccg aaggtagagg ttctctcctc acttgtggtg
     7381 atgttgaaga aaaccctggt ccaatgtcta gactggacaa gagcaaagtc ataaacggag
     7441 ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     7501 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     7561 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     7621 agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac
     7681 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     7741 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     7801 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     7861 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     7921 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     7981 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg
     8041 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     8101 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg
     8161 atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8221 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8281 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8341 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8401 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8461 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8521 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     8581 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8641 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8701 atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta gaggtacctt
     8761 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8821 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     8881 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8941 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9001 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     9061 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9121 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9241 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     9301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt tt
//
