LOCUS       sequence-42199-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00310
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    680..691
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttc
       61 ttataatgcc aactttgtac aagaaagttg gcaataaaac gaaaattgaa ggattataag
      121 atatttccac agctttcaca ttgaactcac tgtgttcaat ttcaaggccg aacaacttaa
      181 ttttgttggc ctcgtgctgc gccttcttct tcaggcgaca ggcgcgtgag gccagcttgt
      241 tcttctcctt gcgcgacttg gggcgcacat tgaatggcag ctcggaaaca ggagtcatgt
      301 cattgatggt gcggcttagc ttgtccagtt ccttgccaat attgtgcagc ttcctggcat
      361 tgggagttaa atcgttacca tcgcccttgc gggccttgcc actgtgcttg tacaccttaa
      421 tgcctcgcac ggagcacgtt ggactgaaga cggggtctgt cacctcgttc agcacatgag
      481 gatcctcgag cttcgattga aagaccagtc tctttccctt gggtcctttg gcctgaacat
      541 tgtactgcca gaaaaatcgc tctttgccct tgctactgga caaatggttt ggtgtcgagg
      601 tacgcgtttc atcttcatcc tcagagagtc cattgtcgct gctacaatcg tcgtagttat
      661 cgctattctc actcgaattg tcatcctcat cgtcctgcga gaagtcaatg ccatccgggg
      721 acaagacact
//
