LOCUS       sequence-422025-       18643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bi-directional luciferase antisense intron (firefly fluc AI) human 
            LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping
            beauty integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18643)
  AUTHORS   Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, 
            Sanchez R, Miller BD, Di Stefano LH, Ruiz FX, Hancock M, Isik E, 
            Mendez-Dorantes C, Walpole T, Nichols C, Wan P, Riento K, Halls-Kass
            R, Augustin M, Lammens A, Jestel A, Upla P, Xibinaku K, Congreve S, 
            Hennink M, Rogala KB, Schneider AM, Fairman JE, Christensen SM, 
            Desrosiers B, Bisacchi GS, Saunders OL, Hafeez N, Miao W, Kapeller 
            R, Zaller DM, Sali A, Weichenrieder O, Burns KH, Gotte M, Rout MP, 
            Arnold E, Greenbaum BD, Romero DL, LaCava J, Taylor MS
  TITLE     Structures, functions and adaptations of the human LINE-1 ORF2 
            protein.
  JOURNAL   Nature. 2024 Feb;626(7997):194-206. doi: 10.1038/s41586-023-06947-z.
            Epub 2023 Dec 14.
  PUBMED    38096902
REFERENCE   2  (bases 1 to 18643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06947-z"; journalName: "Nature"; date: 
            "2024-02"; volume: "626"; issue: "7997"; pages: "194-206"
COMMENT     Sequence Label: pRT006.3 L1 dual-luciferase AI retrotransposit...
FEATURES             Location/Qualifiers
     source          1..18643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..65
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     75..94
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        75..93
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    444..492
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(522..1457)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      1454..1463
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        1512..1899
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     protein_bind    1593..1611
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1629..1647
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1664..1682
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1700..1718
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1736..1754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1771..1789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1807..1825
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    7042..7090
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      9640..9649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9679..10036)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9693..9828
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        9845..10036
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     9993..10013
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    10093..10227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10117..10136)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10171..10190
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(10344..10361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             10727..12646
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="DEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGRGR
                     GRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAGAG
                     GGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGG
                     AGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGGGA
                     GGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAGGG
                     AGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRGGS
                     GGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPPG
                     RRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGRRK
                     KGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYN
                     LRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDA
                     IKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGE
                     EGQE"
     rep_origin      12948..14737
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     rep_origin      complement(14807..15395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14887..14906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15566..16357,16358..16426))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16189..16208
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16427..16531)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      16558..17013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16645..16664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16855..16876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     17154..17170
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    17176..17400
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(17407..18006)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(17490..17510)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     17987..18006
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(18028..18528)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(18091..18109)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
       61 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca
      121 gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta
      181 ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt
      241 gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat
      301 tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt
      361 aaacagcttg gaaaattcca gaaaatgatg tcatggcttt agaagctaga tctggccatc
      421 tagactgcag gcttaagcga tccgaacaaa cgacccaaca cccgtgcgtt ttattctgtc
      481 tttttattgc cgatccccgg tcacccgccc cgactctaga attactgctc gttcttcagc
      541 acgcgctcca cgaagctctt gatgtactta cccatttcat ctggagcgtc ctcctggctg
      601 aagtggaggc ccttcacctt cacgaactcg gtgttaggga acttcttagc tccctcgaca
      661 atagcgttgg aaaagaaccc agggtcggac tcgatgaaca tcttaggcag atcgtcgctg
      721 gcccgaaggt aggcgttgta gttgcggaca atctggacga cgtcgggctt gcctccctta
      781 acgagaggga tctcgcgagg ccaggagagg gtaggccgtc taacctcgcc cttctccttg
      841 aatggctcca ggtaggcagc gaactcctca ggctccagtt tccgcatgat cttgcttggg
      901 agcatggtct cgacgaagaa gttattctca agcaccattt tctcgccctc ttcgctcttg
      961 atcagggcga tatcctcctc gatgtcaggc cactcgtccc aggactcgat cacgtccacg
     1021 acactctcag catggacgat ggccttgatc ttgtcttggt gctcgtagga gtagtgaaag
     1081 gccagacaag ccccccagtc gtggcccaca aagatgattt tctttggaag gttcagcagc
     1141 tcgaaccaag cggtgaggta cttgtagtga tccaggaggc gatatgagcc attcccgctc
     1201 ttgccggact tacccattcc gatcagatca gggatgatgc atctagccac gggctcgatg
     1261 tgaggcacga cgtgcctcca caggtagctg gaggcagcgt taccatgcag aaaaatcacg
     1321 gcgttctcgg cgtgcttctc ggaatcatag tagttgatga aggagtccag cacgttcatt
     1381 tgcttgcagc gagcccacca ctgaggccca gtgatcatgc gtttgcgttg ctcggggtcg
     1441 tacaccttgg aagccatggt ggctttacca acagtaccgg attgccaagc ttatgaccgg
     1501 tgaattctcc aggcgatctg acggttcact aaacgagctc tgcttatata ggcctcccac
     1561 cgtacacgcc acctcgacat actcgagttt actccctatc agtgatagag aacgtatgtc
     1621 gagtttactc cctatcagtg atagagaacg atgtcgagtt tactccctat cagtgataga
     1681 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
     1741 atcagtgata gagaacgtat gtcgagttta tccctatcag tgatagagaa cgtatgtcga
     1801 gtttactccc tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc
     1861 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagatttcg agctcggtac
     1921 ccggggatcc tctagtcagc tgacgcgtgc tagcgcggcc gcatgggcaa gaagcagaac
     1981 cgcaagaccg gcaacagcaa gacccagagc gccagccccc cccccaagga gcgcagcagc
     2041 agccccgcca ccgagcagag ctggatggag aacgacttcg acgagctgcg cgaggagggc
     2101 ttccgccgca gcaactacag cgagctgcgc gaggacatcc agaccaaggg caaggaggtg
     2161 gagaacttcg agaagaacct ggaggagtgc atcacccgca tcaccaacac cgagaagtgc
     2221 ctgaaggagc tgatggagct gaagaccaag gcccgcgagc tgcgcgagga gtgccgcagc
     2281 ctgcgcagcc gctgcgacca attggaggag cgcgtgagcg ccatggagga cgagatgaac
     2341 gagatgaagc gcgagggcaa gttccgcgag aagcgcatca agcgcaacga gcagagcctg
     2401 caggagatct gggactacgt gaagcgcccc aacctgcgcc tgatcggcgt gcccgagagc
     2461 gacgtggaga acggcaccaa gctggagaac accctgcagg acatcatcca ggagaacttc
     2521 cccaacctgg cccgccaggc caacgtgcag atccaggaga tccagcgcac cccccagcgc
     2581 tacagcagcc gccgcgccac cccccgccac atcatcgtgc gcttcaccaa ggtggagatg
     2641 aaggagaaga tgctgcgcgc cgcccgcgag aagggccgcg tgaccctgaa gggcaagccc
     2701 atccgtctca ccgccgacct gagcgccgag accctgcagg cccgccgcga gtggggcccc
     2761 atcttcaaca tcctgaagga gaagaacttc cagccccgca tcagctaccc cgccaagctg
     2821 agcttcatca gcgagggcga gatcaagtac ttcatcgaca agcagatgct gcgcgacttc
     2881 gtgaccaccc gccccgccct gaaggagctg ctgaaggagg ccctgaacat ggagcgcaac
     2941 aaccgctacc agcccctgca gaaccacgcc aagatgtaaa gaccatcgag actaggaaga
     3001 aactgcatca actaatgagc aaaatcaggc gcgcccatca taatgaccgg cagcaacagc
     3061 cacatcacca tcctgaccct gaacatcaac ggcctgaaca gcgccatcaa gcgccaccgc
     3121 ctggccagct ggatcaagag ccaggacccc agcgtgtgct gcatccagga gacccacctg
     3181 acctgccgcg acacccaccg cctgaagatc aagggctggc gcaagatcta ccaggccaac
     3241 ggcaagcaga agaaggccgg cgtggccatc ctggtgagcg acaagaccga cttcaagccc
     3301 accaagatca agcgcgacaa ggagggccac tacatcatgg tgaagggcag catccagcag
     3361 gaggagctga ccatcctgaa catctacgcc cccaacaccg gtgccccccg cttcatcaag
     3421 caggtgctga gcgacctgca gcgcgacctg gacagccaca ccctgatcat gggcgacttc
     3481 aacacccccc tgagcaccct ggaccgcagc acccgccaga aggtgaacaa ggacacccag
     3541 gagctgaaca gcgccctgca ccaggccgac ctgatcgaca tctaccgcac cctgcacccc
     3601 aagagcaccg agtacacctt cttcagcgcc ccccaccaca cctacagcaa gatcgaccac
     3661 atcgtgggca gcaaggccct gctgagcaag tgcaagcgca ccgagatcat caccaactac
     3721 ctgagcgacc acagcgccat caagctggag ctgcgcatca agaacctgac ccagagccgc
     3781 agcaccacct ggaagctgaa caacctgctg ctgaacgact actgggtgca caacgagatg
     3841 aaggccgaga tcaagatgtt cttcgaaacc aacgagaaca aggacaccac ctaccagaac
     3901 ctgtgggacg ccttcaaggc cgtgtgccgc ggcaagttca tcgccctgaa cgcctacaag
     3961 cgcaagcagg agcgcagcaa gatcgacacc ctgaccagcc agctgaagga gctggagaag
     4021 caggagcaga cccacagcaa ggccagccgc cgccaggaga tcaccaagat ccgcgccgag
     4081 ctgaaggaga tcgagaccca gaagaccctg cagaagatca acgagtcgcg aagctggttc
     4141 ttcgagcgca tcaacaagat cgaccgcccc ctggcccgcc tgatcaagaa gaagcgcgag
     4201 aagaaccaga tcgacaccat caagaacgac aagggcgaca tcaccaccga ccccaccgag
     4261 atccagacca ccatccgcga gtactacaag cacctgtacg ccaacaagct ggagaacctg
     4321 gaggagatgg acaccttcct ggacacctac accctgcccc gcctgaacca ggaggaggtg
     4381 gagagcctga accgccccat caccggcagc gagatcgtgg ccatcatcaa cagcctgccc
     4441 accaagaaga gccccggccc cgacggcttc accgccgagt tctaccagcg ctacaaggag
     4501 gagctggtgc ccttcctgct gaagctgttc caaagcatcg agaaggaggg catcctgccc
     4561 aacagcttct acgaggccag catcatcctg atccccaagc ccggccgcga caccaccaag
     4621 aaggagaact tccgccccat cagcctgatg aacatcgacg ccaagatcct gaacaagatc
     4681 ctggccaacc gcatccagca gcacatcaag aagctgatcc accacgacca ggtgggcttc
     4741 atccccggga tgcagggctg gttcaacatc cgcaagagca tcaacgtgat ccagcacatc
     4801 aaccgcgcca aggacaagaa ccacatgatc atcagcatcg acgccgagaa ggccttcgac
     4861 aagatccagc agcccttcat gctgaagacc ctgaacaagc tgggcatcga cggcacctac
     4921 ttcaagatca tccgcgccat ctacgacaag cccaccgcca acatcatcct gaacggccag
     4981 aagctggagg ccttccccct gaagaccggc acgcgtcagg gctgccccct gagccccctg
     5041 ctgttcaaca tcgtgctgga ggtgctggcc cgcgccatcc gccaggagaa ggagatcaag
     5101 ggcatccagc tgggcaagga ggaggtgaag ctgagcctgt tcgccgacga catgatcgtg
     5161 tacctggaga accccatcgt gagcgcccag aacctgctga agctgatcag caacttcagc
     5221 aaggtgagcg gctacaagat caacgtgcag aagagccagg ccttcctgta caccaacaac
     5281 cgccagaccg agagccagat catgggcgag ctgcccttca ccatcgctag caagcgcatc
     5341 aagtacctgg gcatccagct gacccgcgac gtgaaggacc tgttcaagga gaactacaag
     5401 cccctgctga aggagatcaa ggaggagacc aacaagtgga agaacatccc ctgcagctgg
     5461 gtgggccgca tcaacatcgt gaagatggcc atcctgccca aggtgatcta ccgcttcaac
     5521 gccatcccca tcaagctgcc catgaccttc ttcaccgagc tggagaagac caccctgaag
     5581 ttcatctgga accagaagcg cgcccgcatc gccaagagca tcctgagcca gaagaacaag
     5641 gccggcggca tcaccctgcc cgacttcaag ctgtactaca aggccaccgt gaccaagacc
     5701 gcctggtact ggtaccagaa ccgcgatatc gaccagtgga accgcaccga gcccagcgag
     5761 atcatgcccc acatctacaa ctacctgatc ttcgacaagc ccgagaagaa caagcagtgg
     5821 ggcaaggaca gcctgttcaa caagtggtgc tgggagaact ggctggccat ctgccgcaag
     5881 ctgaagctgg accccttcct gaccccctac accaagatca acagccgctg gatcaaggac
     5941 ctgaacgtga agcccaagac catcaagacc ctggaggaga acctgggcat caccatccag
     6001 gacatcggcg tgggcaagga cttcatgagc aagaccccca aggccatggc caccaaggac
     6061 aagatcgaca agtgggacct gatcaagctg aagagcttct gcaccgccaa ggagaccacc
     6121 atccgcgtga accgccagcc caccacctgg gagaagatct tcgccaccta cagcagcgac
     6181 aagggcctga tcagccgcat ctacaacgag ctgaagcaga tctacaagaa gaagaccaac
     6241 aaccccatca agaagtgggc caaggacatg aaccgccact tcagcaagga ggacatctac
     6301 gccgccaaga agcatatgaa gaagtgcagc agcagcctgg ccatccgcga gatgcagatc
     6361 aagaccacca tgcgctacca cctgaccccc gtgcgcatgg ccatcatcaa gaagagcggc
     6421 aacaaccgct gctggcgcgg ctgcggcgag atcggcaccc tgctgcactg ctggtgggac
     6481 tgcaagctgg tgcagcccct gtggaagagc gtgtggcgct tcctgcgcga cctggagctg
     6541 gagatcccct tcgaccccgc catccccctg ctgggcatct accccaacga gtacaagagc
     6601 tgctgctaca aggacacctg cacccgcatg ttcatcgccg ccctgttcac catcgccaag
     6661 acctggaacc agcccaagtg ccccaccatg atcgattgga tcaagaagat gtggcacatc
     6721 tacaccatgg agtactacgc cgccatcaag aacgacgagt tcatcagctt cgtgggcacc
     6781 tggatgaagc tggagaccat catcctgagc aagctgagcc aggagcagaa gaccaagcac
     6841 cgcatcttca gcctgatcgg cggcaactga acaatgagat cacatggaca caggaagggg
     6901 aatatcacac tctggggact gtggtggggt cgggggaggg gggagggata gcattgggag
     6961 atatacctaa tgctagatga cacattagtg ggtgcagcgc accagcatgg cacatgtaat
     7021 ccctgcaggc ttaagcgatc cgaacaaacg acccaacacc cgtgcgtttt attctgtctt
     7081 tttattgccg atccccggtc acccgccccg actctagaat tattacacgg cgatcttgcc
     7141 gcccttcttg gccttaatga gaatctcgcg gatcttgcgg gcgtccaact tgccggtcag
     7201 tcctttaggc acctcgtcca cgaacacaac accaccgcgc agcttcttgg cggttgtaac
     7261 ctggctggcc acatagtcca cgatctcctt ctcggtcatg gttttaccgt gttccagcac
     7321 gacgactgcg gcgggcagct cgccggcatc gtcgtcgggc aggccggcga ccccggcgtc
     7381 gaagatgttg gggtgttgca gcaggatgct ctccagttcg gctggggcta cctggtagcc
     7441 cttgtatttg atcaggctct tcagccggtc cacgatgaag aagtgctcgt cctcgtccca
     7501 gtaggcgatg tcgccgctgt gcagccagcc gtccttgtcg atgagagcgt ttgtagcctc
     7561 ggggttgtta acgtagccgc tcatgatcat ggggccacgg acgcacagct cgccgcgctg
     7621 gttcacaccc agtgtcttac cggtgtccaa gtccaccacc ttagcctcga agaagggcac
     7681 caccttgcct actgcgccag gcttgtcgtc cccttcgggg gtgatcagaa tggcgctggt
     7741 tgtttctgtc aggccgtagc cctggcggat gcctggtagg tggaagcgtt tggccacggc
     7801 ctcacctacc tccttgctga gcggcgcccc gccgctggcg atctcgtgca aattgcttag
     7861 gtcgtacttg tcgatgagag tgctcttagc gaagaagcta aatagtgtgg gcaccagcag
     7921 ggcagattga atcttatagt cttgcaagct gcgcaagaat agctcctcct cgaagcggta
     7981 catgagcacg acccgaaagc cgcagatcaa gtagcccagc gtggtgaaca tgccgaagcc
     8041 gtggtgaaat ggcaccacgc tgaggatagc ggtgtcgggg atgatctggt tgccgaagat
     8101 ggggtcgcgg gcatgactga atcggacaca agcggtgcgg tgcggtaggg ctacgccctt
     8161 gggcaatccg gtactgccac tactgttcat gatcagggcg atggttttgt cccggtcgaa
     8221 gctctcgggc acgaagtcgt actcgttgaa gccgggtggc aggtgagtcc aggagatgtt
     8281 tcagcactgt tgcctttagt ctcgaggcaa cttagacaac tgagtattga tctgagcaca
     8341 gcagggtgtg agctgtttga agatactggg gttgggggtg aagaaactgc agaggactaa
     8401 ctgggctgag acccagtggc aatgttttag ggcctaagga atgcctctga aaatctagat
     8461 ggacaacttt gactttgaga aaagagaggt ggaaatgagg aaaatgactt ttctttatta
     8521 gatttcggta gaaagaactt tcatctttcc cctatttttg ttattcgttt taaaacatct
     8581 atctggaggc aggacaagta tggtcattaa aaagatgcag gcagaaggca tatattggct
     8641 cagtcaaagt ggggaacttt ggtggccaaa catacattgc taaggctatt cctatatcag
     8701 ctggacacat ataaaatgct gctaatgctt cattacaaac ttatatcctt taattccaga
     8761 tgggggcaaa gtatgtccag gggtgaggaa caattgaaac atttgggctg gagtagattt
     8821 tgaaagtcag ctctgtgtgt gtgtgtgtgt gtgtgtgtgt gagagcgtgt gtttctttta
     8881 acgttttcag cctacagcat acagggttta tggtggcaag aagataacaa gatttaaatt
     8941 atggccagtg actagtgctg caagaagaac aactacctgc atttaatggg aaagcaaaat
     9001 ctcaggcttt gagggaagtt aacataggct tgattctggg tggaagctgg gtgtgtagtt
     9061 atctggaggc caggctggag ctctcagctc actatgggtt catctttatt gtctcctttc
     9121 atctcaacag gtgggaagtc acgaaggtgt acatgctttg gaagccctgg tagtcggtct
     9181 tgctatccat gatgatgatc ttttgtatga tcggtagctt cttttgcacg ttgaggatct
     9241 tttgcagccc tttcttgctc acgaatacga cggtgggctg gctgatgccc atgctgttca
     9301 gcagctcgcg ctcgttgtag atgtcgttag ctggggccac agccacaccg atgaacaggg
     9361 cacccaacac gggcatgaag aactgcaagc tattctcgct gcacaccacg atccgatggt
     9421 ttgtattcag cccatagcgc ttcatagctt ctgccagccg aacgctcatc tcgaagtact
     9481 cggcgtaggt aatgtccacc tcgatatgtg cgtcggtaaa ggcgatggtg ccgggcacca
     9541 gggcgtagcg cttcatggct ttgtgcagct gctcgccggc ggtcccgtct tcgagtgggt
     9601 agaatggcgc tgggcccttc ttaatgtttt tggcatcttc catggtggct ttaccaacag
     9661 taccggattg ccaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     9721 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     9781 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     9841 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     9901 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     9961 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
    10021 gttctttccg cctcagaagg tacctaacca agttcctctt tcagaggtta tttcaggcca
    10081 tggtgctgcg cagatccaga catgataaga tacattgatg agtttggaca aaccacaact
    10141 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
    10201 accattataa gctgcaataa acaagttatt gcgttgcgct cactgcccgc tttccagtcg
    10261 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    10321 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    10381 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    10441 aacgcaggaa agaattgagt gtatgtaaac ttctgaccca ctgggaatgt gatgaaagaa
    10501 ataaaagctg aaatgaatca ttctctctac tattattctg atatttcaca ttcttaaaat
    10561 aaagtggtga tcctaactga cctaagacag ggaattttta ctaggattaa atgtcaggaa
    10621 ttgtgaaaaa gtgagtttaa atgtatttgg ctaaggtgta tgtaaacttc cgacttcaac
    10681 tgtataggga tcctctagct agagtcggta tacgtttaaa cgtcgagacg aggggccagg
    10741 tacaggacct ggaaatggcc taggagagaa gggagacaca tctggaccag aaggctccgg
    10801 cggcagtgga cctcaaagaa gagggggtga taaccatgga cgaggacggg gaagaggacg
    10861 aggacgagga ggcggaagac caggagcccc gggcggctca ggatcagggc caagacatag
    10921 agatggtgtc cggagacccc aaaaacgtcc aagttgcatt ggctgcaaag ggacccacgg
    10981 tggaacagga gcaggagcag gagcgggagg ggcaggagca ggaggggcag gagcaggagg
    11041 aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagggg caggagcagg
    11101 aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggagg
    11161 ggcaggagca ggaggagggg caggaggggc aggagcagga ggaggggcag gaggggcagg
    11221 aggggcagga gcaggaggag gggcaggagc aggaggaggg gcaggagggg caggagcagg
    11281 aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggggc
    11341 aggaggggca ggaggggcag gagcaggagg ggcaggagca ggaggagggg caggaggggc
    11401 aggaggggca ggagcaggag gggcaggagc aggaggggca ggagcaggag gggcaggagc
    11461 aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg
    11521 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc
    11581 aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg
    11641 ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggggcaggag caggaggtgg
    11701 aggccggggt cgaggaggca gtggaggccg gggtcgagga ggtagtggag gccggggtcg
    11761 aggaggtagt ggaggccgcc ggggtagagg acgtgaaaga gccagggggg gaagtcgtga
    11821 aagagccagg gggagaggtc gtggacgtgg agaaaagagg cccaggagtc ccagtagtca
    11881 gtcatcatca tccgggtctc caccgcgcag gccccctcca ggtagaaggc catttttcca
    11941 ccctgtaggg gaagccgatt attttgaata ccaccaagaa ggtggcccag atggtgagcc
    12001 tgacgtgccc ccgggagcga tagagcaggg ccccgcagat gacccaggag aaggcccaag
    12061 cactggaccc cggggtcagg gtgatggagg caggcgcaaa aaaggagggt ggtttggaaa
    12121 gcatcgtggt caaggaggtt ccaacccgaa atttgagaac attgcagaag gtttaagagc
    12181 tctcctggct aggagtcacg tagaaaggac taccgacgaa ggaacttggg tcgccggtgt
    12241 gttcgtatat ggaggtagta agacctccct ttacaaccta aggcgaggaa ctgcccttgc
    12301 tattccacaa tgtcgtctta caccattgag tcgtctcccc tttggaatgg cccctggacc
    12361 cggcccacaa cctggcccgc taagggagtc cattgtctgt tatttcatgg tctttttaca
    12421 aactcatata tttgctgagg ttttgaagga tgcgattaag gaccttgtta tgacaaagcc
    12481 cgctcctacc tgcaatatca gggtgactgt gtgcagcttt gacgatggag tagatttgcc
    12541 tccctggttt ccacctatgg tggaaggggc tgccgcggag ggtgatgacg gagatgacgg
    12601 agatgaagga ggtgatggag atgagggtga ggaagggcag gagtgatgta acttgttagg
    12661 agacgccctc aatcgtatta aaagccgtgt attcccccgc actaaagaat aaatccccag
    12721 tagacatcat gcgtgctgtt ggtgtatttc tggccatctg tcttgtcacc attttcgtcc
    12781 tcccaacatg gggcaattgg gcatacccat gttgtcacgt cactcagctc cgcgctcaac
    12841 accttctcgc gttggaaaac attagcgaca tttacctggt gagcaatcag acatgcgacg
    12901 gctttagcct ggcctcctta aattcaccta agaatgggag caaccagcag gaaaaggaca
    12961 agcagcgaaa attcacgccc ccttgggagg tggcggcata tgcaaaggat agcactccca
    13021 ctctactact gggtatcata tgctgactgt atatgcatga ggatagcata tgctacccgg
    13081 atacagatta ggatagcata tactacccag atatagatta ggatagcata tgctacccag
    13141 atatagatta ggatagccta tgctacccag atataaatta ggatagcata tactacccag
    13201 atatagatta ggatagcata tgctacccag atatagatta ggatagccta tgctacccag
    13261 atatagatta ggatagcata tgctacccag atatagatta ggatagcata tgctatccag
    13321 atatttgggt agtatatgct acccagatat aaattaggat agcatatact accctaatct
    13381 ctattaggat agcatatgct acccggatac agattaggat agcatatact acccagatat
    13441 agattaggat agcatatgct acccagatat agattaggat agcctatgct acccagatat
    13501 aaattaggat agcatatact acccagatat agattaggat agcatatgct acccagatat
    13561 agattaggat agcctatgct acccagatat agattaggat agcatatgct atccagatat
    13621 ttgggtagta tatgctaccc atggcaacat tagcccaccg tgctctcagc gacctcgtga
    13681 atatgaggac caacaaccct gtgcttggcg ctcaggcgca agtgtgtgta atttgtcctc
    13741 cagatcgcag caatcgcgcc cctatcttgg cccgcccacc tacttatgca ggtattcccc
    13801 ggggtgccat tagtggtttt gtgggcaagt ggtttgaccg cagtggttag cggggttaca
    13861 atcagccaag ttattacacc cttattttac agtccaaaac cgcagggcgg cgtgtggggg
    13921 ctgacgcgtg cccccactcc acaatttcaa aaaaaagagt ggccacttgt ctttgtttat
    13981 gggccccatt ggcgtggagc cccgtttaat tttcgggggt gttagagaca accagtggag
    14041 tccgctgctg tcggcgtcca ctctctttcc ccttgttaca aatagagtgt aacaacatgg
    14101 ttcacctgtc ttggtccctg cctgggacac atcttaataa ccccagtatc atattgcact
    14161 aggattatgt gttgcccata gccataaatt cgtgtgagat ggacatccag tctttacggc
    14221 ttgtccccac cccatggatt tctattgtta aagatattca gaatgtttca ttcctacact
    14281 agtatttatt gcccaagggg tttgtgaggg ttatattggt gtcatagcac aatgccacca
    14341 ctgaaccccc cgtccaaatt ttattctggg ggcgtcacct gaaaccttgt tttcgagcac
    14401 ctcacataca ccttactgtt cacaactcag cagttattct attagctaaa cgaaggagaa
    14461 tgaagaagca ggcgaagatt caggagagtt cactgcccgc tccttgatct tcagccactg
    14521 cccttgtgac taaaatggtt cactaccctc gtggaatcct gaccccatgt aaataaaacc
    14581 gtgacagctc atggggtggg agatatcgct gttccttagg acccttttac taaccctaat
    14641 tcgatagcat atgcttcccg ttgggtaaca tatgctattg aattagggtt agtctggata
    14701 gtatatacta ctacccggga agcatatgct acccgttcgc gatcgccatg tgagcaaaag
    14761 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    14821 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    14881 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    14941 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    15001 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    15061 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    15121 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    15181 gagcaaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    15241 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    15301 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    15361 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    15421 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    15481 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    15541 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    15601 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    15661 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    15721 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    15781 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    15841 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    15901 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    15961 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    16021 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    16081 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    16141 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    16201 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    16261 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    16321 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    16381 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    16441 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    16501 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    16561 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    16621 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    16681 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    16741 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
    16801 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    16861 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
    16921 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
    16981 cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg
    17041 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    17101 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    17161 gacggccagt gagcgccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
    17221 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
    17281 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
    17341 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
    17401 ggtacctcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc
    17461 gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga
    17521 ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac
    17581 ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca
    17641 cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag
    17701 ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc
    17761 gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt
    17821 gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat
    17881 gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag
    17941 ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc
    18001 ggtcatggta agctccggtg gatccccctg gggagagagg tcggtgattc ggtcaacgag
    18061 ggagccgact gccgacgtgc gctccggagg cttgcagaat gcggaacacc gcgcgggcag
    18121 gaacagggcc cacactaccg ccccacaccc cgcctcccgc accgcccctt cccggccgct
    18181 gctctcggcg cgccctgctg agcagccgct attggccaca gcccatcgcg gtcggcgcgc
    18241 tgccattgct ccctggcgct gtccgtctgc gagggtacta gtgagacgtg cggcttccgt
    18301 ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg gcggagcagg
    18361 aagcgtcgcc ggggggccca caagggtagc ggcgaagatc cgggtgacgc tgcgaacgga
    18421 cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac cacgcccaga
    18481 gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaacc ccaagacgag
    18541 gggccaggta caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa
    18601 ggctccggcg gcagtggacc tcaaagaaga gggggtgata acc
//
