LOCUS       sequence-422048-       10013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin conformation sensor (VcnCS) with both directionally 
            asymmetric, force strengthening (DAFS) variant point mutations 
            (E1015A and E1021A)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Chirasani VR, Khan MAI, Malavade JN, Dokholyan NV, Hoffman BD, 
            Campbell SL
  TITLE     Molecular basis and cellular functions of vinculin-actin directional
            catch bonding.
  JOURNAL   Nat Commun. 2023 Dec 14;14(1):8300. doi: 10.1038/s41467-023-43779-x.
  PUBMED    38097542
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-12-14"; volume: "14(1):8300. doi"; pages: " 
            10.1038/s41467-023-43779-x"
COMMENT     Sequence Label: pcDNA3.1-VcnCS-E1015A-E1021A
FEATURES             Location/Qualifiers
     source          1..10013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     650..669
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    954..1075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(991..1010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1045..1064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1124..1140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1124..1140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1137..1159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1148..1164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1172..1178,1179..1196,1197..1202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1217..1238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1355..1372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1526..2114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1606..1625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2285..3076,3077..3145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2908..2927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3146..3250)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3325..3344)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3516..3895
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3896..4099
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4050..4070
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4144..4163
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4144..4162
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6856..7563
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(8128..8130,8131..8133,8134..8847)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8173..8194)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8434..8453)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8781..8802
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8885..8902)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8891..9115
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9161..9589
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9248..9267)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9458..9479
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9598..9618)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9603..9932
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9783..9918
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9845..9864
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             join(9999..10013,1..780)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
       61 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
      121 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
      181 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
      241 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
      301 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
      361 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
      421 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
      481 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
      541 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
      601 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
      661 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
      721 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
      781 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
      841 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
      901 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
      961 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1021 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1081 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     1141 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1201 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1261 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1321 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3241 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     3301 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     3361 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     3421 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     3481 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     3541 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     3601 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     3661 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     3721 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     3781 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     3841 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     3901 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     3961 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     4021 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     4081 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
     4141 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttgcc
     4201 atgcccgtct tccacacgcg caccatcgag agcatcttgg agcccgtggc tcagcagatc
     4261 tcccacctgg tcatcatgca cgaggagggg gaggtagacg gcaaggccat cccggacctc
     4321 accgcccccg tgtcggccgt gcaggccgct gtcagcaacc tggtgcgggt tggaaaagaa
     4381 actgtgcaga caacagaaga ccagatcttg aaaagggata tgccaccagc attcatcaaa
     4441 gtagagaatg cctgcaccaa gctcgttcga gcagcccaga tgctgcaagc agatccttat
     4501 tcagtaccag ctcgtgacta cctaattgat ggatcaagag gcatcctttc tggaacatca
     4561 gacttacttc tgacatttga tgaagcggag gtccgtaaaa tcatccgtgt ctgcaaagga
     4621 atattggaat atctgactgt ggcagaagta gtagagacta tggaggattt ggtgacatat
     4681 acaaagaatc tagggccagg aatgacaaag atggcgaaaa tgatcgatga gagacaacag
     4741 gaattaactc atcaggaaca tagggttatg ctggtgaact ccatgaatac tgtgaaggag
     4801 ctattgcctg tacttatttc agctatgaag atctttgtaa ccacaaaaaa cactaaaagc
     4861 cagggaatag aagaggcctt gaaaaatcgc aatttcacag tagagaaaat gagtgctgag
     4921 ataaatgaaa taatccgtgt attacaactc acttcctggg atgaagatgc ctgggccagc
     4981 aaggacactg aagccatgaa aagagcttta gccctaatag attcaaagat gaaccaggca
     5041 aaaggctggc tgagagatcc aaacgcacct ccaggggatg ctggtgagca agcaataagg
     5101 cagatccttg atgaagctgg aaaagcagga gaattgtgtg caggcaaaga acgcagggag
     5161 attctgggaa catgcaaaac tttgggccaa atgactgatc aacttgctga tctccgagct
     5221 agaggacagg gtgctacacc gatggcaatg cagaaggcac agcaggtgtc acaaggcctg
     5281 gatttgctca ctgcaaaagt ggagaatgca gcccggaaat tggaggccat gacaaactct
     5341 aagcaggcta ttgcaaagaa gattgatgct gctcagaact ggcttgcgga tcccaacggc
     5401 ggaagtgaag gagaagaaca cattcgagga attatgtctg aagcaaggaa agttgcagaa
     5461 ttgtgtgagg agcctaaaga aagagatgat atccttcgct ccttggggga aatctctgct
     5521 ctgacagcta agctgtcaga tctgcgacga catgggaaag gcgactctcc tgaggcccgt
     5581 gcattggcca agcaaatagc tacatcactt cagaacttac agtccaaaac aaacagagct
     5641 gtagcaaata ctaggccagt taaagctgct gtccatttgg agggcaagat tgagcaagcc
     5701 cagaggtgga tagataatcc tacagttgat gatcggggag taggccaggc agcaattcgg
     5761 ggtttggttg cagaaggtcg tcgtctagcc aatgtcatga tgggacctta tcgtcaggac
     5821 ctgcttgcca aatgtgaccg tgtagaccag ctggctgctc agcttgctga ccttgcagca
     5881 agaggggaag gagagtctcc tcaggctagg gcaattgctg ctcagcttca ggactccctg
     5941 aaggatctca aagcacggat gcaagaagca atgacccagg aggtgtctga tgttttcagt
     6001 gacactacaa cccctattaa gttgttagca gtagcagcca ctgctccttc tgatactccc
     6061 aatagagaag aggtgtttga agaaagagca gcaaattttg aaaaccatgc tgctagactg
     6121 ggagcaacag cagaaaaagc agctgcagtt ggaactgcta ataaaactac tgtggaaggc
     6181 attcaggcaa cagtcaaatc tgcaagggag cttacaccac aggtagtatc ggctgctcga
     6241 atcctcctga gaaatcctgg aaatcaagct gcttatgagc attttgagac aatgaaaaac
     6301 caatggattg ataatgtaga aaagatgaca gggctggtgg atgaggccat cgataccaag
     6361 tctctgttgg atgcatcaga agaggctatt aagaaggatc ttgataaatg taaagttgca
     6421 atggccaata tgcaacctca gatgctggta gctggagcca ccagcattgc tagacgagcg
     6481 aaccgcatcc tgcttgtggc aaaacgggag gttgaaaatt cagaagaccc taaattcagg
     6541 gaggctgtta aagcagcttc tgatgagctg agcaaaacca tatcaccgat ggtaatggat
     6601 gctaaagctg tagcaggaaa tatctctgat cctggtttgc agaagagttt cttggattct
     6661 ggatacagga ttctgggagc tgtggccaaa gtcagagaag cctttcagcc tcaggagcca
     6721 gactttcccc ctcctcctcc tgacctagag catctccatc tgactgatga gcttgctcct
     6781 ccaaaaccac cacttccaga aggtgaggtt cccccaccca gaccaccacc ccctgaagaa
     6841 aaagatgagg tcgagatggt gagcaagggc gaggagacca caatgggcgt aatcaagccc
     6901 gacatgaaga tcaagctgaa gatggagggc aacgtgaatg gccacgcctt cgtgatcgag
     6961 ggcgagggcg agggcaagcc ctacgacggc accaacacca tcaacctgga ggtgaaggag
     7021 ggagcccccc tgcccttctc ctacgacatt ctgaccaccg cgttcgccta cggcaacagg
     7081 gccttcacca agtaccccga cgacatcccc aactacttca agcagtcctt ccccgagggc
     7141 tactcttggg agcgcaccat gaccttcgag gacaagggca tcgtgaaggt gaagtccgac
     7201 atctccatgg aggaggactc cttcatctac gagatacacc tcaagggcga gaacttcccc
     7261 cccaacggcc ccgtgatgca gaagaagacc accggctggg acgcctccac cgagaggatg
     7321 tacgtgcgcg acggcgtgct gaagggcgac gtcaagcaca agctgctgct ggagggcggc
     7381 ggccaccacc gcgttgactt caagaccatc tacagggcca agaaggcggt gaagctgccc
     7441 gactatcact ttgtggacca ccgcatcgag atcctgaacc acgacaagga ctacaacaag
     7501 gtgaccgttt acgagagcgc cgtggcccgc aactccaccg acggcatgga cgagctgtac
     7561 aaggcggccg cggagttccc agagcagaaa gcaggagaag ctattaatca gcccatgatg
     7621 atggctgcta ggcagttgca tgacgaggct cggaaatggt ctagcaaggg taacgacatc
     7681 attgctgctg ctaaacgaat ggcgctgctc atggctgaga tgtcacgcct ggtgcgagga
     7741 ggcagcggaa acaagcgtgc cctcatccag tgtgcaaaag atattgctaa ggcatcggat
     7801 gaagtcactc gattggccaa agaggtggcc aagcaatgta ctgataaacg cattagaaca
     7861 aacctcttac aggtctgtga gcgaatccca accatcagca cgcagctcaa aattctttcc
     7921 acagtgaaag ctaccatgct gggcaggact aacatcagcg atgaagcttc agaacaggca
     7981 actgccatgt tggttcataa cgcccagaac ctcatgcagt ctgtgaagga aactgtgaga
     8041 gaagctgaag cagcatccat taagataaga acagatgccg gattcactct gcgctgggtc
     8101 agaaagaccc catggtatca ggaattcatg gtgagcaagg gcgaggagct gttcaccggg
     8161 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     8221 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     8281 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc
     8341 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     8401 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     8461 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     8521 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     8581 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     8641 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     8701 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaaact gagcaaagac
     8761 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     8821 ctcggcatgg acgagctgta caagtaaatc gattctagag ggcccgttta aacccgctga
     8881 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     8941 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     9001 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     9061 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     9121 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     9181 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9241 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9301 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9361 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9421 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9481 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     9541 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     9601 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9661 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     9721 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     9781 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9841 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     9901 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     9961 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gat
//
