LOCUS       sequence-422050-       14044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human LINE-1 ORF2 only in human cells (codon optimized 
            ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, 
            Sanchez R, Miller BD, Di Stefano LH, Ruiz FX, Hancock M, Isik E, 
            Mendez-Dorantes C, Walpole T, Nichols C, Wan P, Riento K, Halls-Kass
            R, Augustin M, Lammens A, Jestel A, Upla P, Xibinaku K, Congreve S, 
            Hennink M, Rogala KB, Schneider AM, Fairman JE, Christensen SM, 
            Desrosiers B, Bisacchi GS, Saunders OL, Hafeez N, Miao W, Kapeller 
            R, Zaller DM, Sali A, Weichenrieder O, Burns KH, Gotte M, Rout MP, 
            Arnold E, Greenbaum BD, Romero DL, LaCava J, Taylor MS
  TITLE     Structures, functions and adaptations of the human LINE-1 ORF2 
            protein.
  JOURNAL   Nature. 2024 Feb;626(7997):194-206. doi: 10.1038/s41586-023-06947-z.
            Epub 2023 Dec 14.
  PUBMED    38096902
REFERENCE   2  (bases 1 to 14044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06947-z"; journalName: "Nature"; date: 
            "2024-02"; volume: "626"; issue: "7997"; pages: "194-206"
COMMENT     Sequence Label: pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, ...
FEATURES             Location/Qualifiers
     source          1..14044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..608
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4514..4537
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4538..4603
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4813..4947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4837..4856)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4891..4910
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5090..5112)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5676..7465
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(7767..9692)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGXGGGAGGAGGAGAGGGAGAGGGAGGAGXGGGX
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(10127..10145)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10213..10317
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10318..10386,10387..11178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10536..10555)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11349..11937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11838..11857
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        12132..12631
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(12167..12190)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     12564..12583
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             12671..13270
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12671..12690)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13167..13187
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc ccgcgcccat
      661 cataatgacc ggcagcaaca gccacatcac catcctgacc ctgaacatca acggcctgaa
      721 cagcgccatc aagcgccacc gcctggccag ctggatcaag agccaggacc ccagcgtgtg
      781 ctgcatccag gagacccacc tgacctgccg cgacacccac cgcctgaaga tcaagggctg
      841 gcgcaagatc taccaggcca acggcaagca gaagaaggcc ggcgtggcca tcctggtgag
      901 cgacaagacc gacttcaagc ccaccaagat caagcgcgac aaggagggcc actacatcat
      961 ggtgaagggc agcatccagc aggaggagct gaccatcctg aacatctacg cccccaacac
     1021 cggtgccccc cgcttcatca agcaggtgct gagcgacctg cagcgcgacc tggacagcca
     1081 caccctgatc atgggcgact tcaacacccc cctgagcacc ctggaccgca gcacccgcca
     1141 gaaggtgaac aaggacaccc aggagctgaa cagcgccctg caccaggccg acctgatcga
     1201 catctaccgc accctgcacc ccaagagcac cgagtacacc ttcttcagcg ccccccacca
     1261 cacctacagc aagatcgacc acatcgtggg cagcaaggcc ctgctgagca agtgcaagcg
     1321 caccgagatc atcaccaact acctgagcga ccacagcgcc atcaagctgg agctgcgcat
     1381 caagaacctg acccagagcc gcagcaccac ctggaagctg aacaacctgc tgctgaacga
     1441 ctactgggtg cacaacgaga tgaaggccga gatcaagatg ttcttcgaaa ccaacgagaa
     1501 caaggacacc acctaccaga acctgtggga cgccttcaag gccgtgtgcc gcggcaagtt
     1561 catcgccctg aacgcctaca agcgcaagca ggagcgcagc aagatcgaca ccctgaccag
     1621 ccagctgaag gagctggaga agcaggagca gacccacagc aaggccagcc gccgccagga
     1681 gatcaccaag atccgcgccg agctgaagga gatcgagacc cagaagaccc tgcagaagat
     1741 caacgagtcg cgaagctggt tcttcgagcg catcaacaag atcgaccgcc ccctggcccg
     1801 cctgatcaag aagaagcgcg agaagaacca gatcgacacc atcaagaacg acaagggcga
     1861 catcaccacc gaccccaccg agatccagac caccatccgc gagtactaca agcacctgta
     1921 cgccaacaag ctggagaacc tggaggagat ggacaccttc ctggacacct acaccctgcc
     1981 ccgcctgaac caggaggagg tggagagcct gaaccgcccc atcaccggca gcgagatcgt
     2041 ggccatcatc aacagcctgc ccaccaagaa gagccccggc cccgacggct tcaccgccga
     2101 gttctaccag cgctacaagg aggagctggt gcccttcctg ctgaagctgt tccaaagcat
     2161 cgagaaggag ggcatcctgc ccaacagctt ctacgaggcc agcatcatcc tgatccccaa
     2221 gcccggccgc gacaccacca agaaggagaa cttccgcccc atcagcctga tgaacatcga
     2281 cgccaagatc ctgaacaaga tcctggccaa ccgcatccag cagcacatca agaagctgat
     2341 ccaccacgac caggtgggct tcatccccgg gatgcagggc tggttcaaca tccgcaagag
     2401 catcaacgtg atccagcaca tcaaccgcgc caaggacaag aaccacatga tcatcagcat
     2461 cgacgccgag aaggccttcg acaagatcca gcagcccttc atgctgaaga ccctgaacaa
     2521 gctgggcatc gacggcacct acttcaagat catccgcgcc atctacgaca agcccaccgc
     2581 caacatcatc ctgaacggcc agaagctgga ggccttcccc ctgaagaccg gcacgcgtca
     2641 gggctgcccc ctgagccccc tgctgttcaa catcgtgctg gaggtgctgg cccgcgccat
     2701 ccgccaggag aaggagatca agggcatcca gctgggcaag gaggaggtga agctgagcct
     2761 gttcgccgac gacatgatcg tgtacctgga gaaccccatc gtgagcgccc agaacctgct
     2821 gaagctgatc agcaacttca gcaaggtgag cggctacaag atcaacgtgc agaagagcca
     2881 ggccttcctg tacaccaaca accgccagac cgagagccag atcatgggcg agctgccctt
     2941 caccatcgct agcaagcgca tcaagtacct gggcatccag ctgacccgcg acgtgaagga
     3001 cctgttcaag gagaactaca agcccctgct gaaggagatc aaggaggaga ccaacaagtg
     3061 gaagaacatc ccctgcagct gggtgggccg catcaacatc gtgaagatgg ccatcctgcc
     3121 caaggtgatc taccgcttca acgccatccc catcaagctg cccatgacct tcttcaccga
     3181 gctggagaag accaccctga agttcatctg gaaccagaag cgcgcccgca tcgccaagag
     3241 catcctgagc cagaagaaca aggccggcgg catcaccctg cccgacttca agctgtacta
     3301 caaggccacc gtgaccaaga ccgcctggta ctggtaccag aaccgcgata tcgaccagtg
     3361 gaaccgcacc gagcccagcg agatcatgcc ccacatctac aactacctga tcttcgacaa
     3421 gcccgagaag aacaagcagt ggggcaagga cagcctgttc aacaagtggt gctgggagaa
     3481 ctggctggcc atctgccgca agctgaagct ggaccccttc ctgaccccct acaccaagat
     3541 caacagccgc tggatcaagg acctgaacgt gaagcccaag accatcaaga ccctggagga
     3601 gaacctgggc atcaccatcc aggacatcgg cgtgggcaag gacttcatga gcaagacccc
     3661 caaggccatg gccaccaagg acaagatcga caagtgggac ctgatcaagc tgaagagctt
     3721 ctgcaccgcc aaggagacca ccatccgcgt gaaccgccag cccaccacct gggagaagat
     3781 cttcgccacc tacagcagcg acaagggcct gatcagccgc atctacaacg agctgaagca
     3841 gatctacaag aagaagacca acaaccccat caagaagtgg gccaaggaca tgaaccgcca
     3901 cttcagcaag gaggacatct acgccgccaa gaagcatatg aagaagtgca gcagcagcct
     3961 ggccatccgc gagatgcaga tcaagaccac catgcgctac cacctgaccc ccgtgcgcat
     4021 ggccatcatc aagaagagcg gcaacaaccg ctgctggcgc ggctgcggcg agatcggcac
     4081 cctgctgcac tgctggtggg actgcaagct ggtgcagccc ctgtggaaga gcgtgtggcg
     4141 cttcctgcgc gacctggagc tggagatccc cttcgacccc gccatccccc tgctgggcat
     4201 ctaccccaac gagtacaaga gctgctgcta caaggacacc tgcacccgca tgttcatcgc
     4261 cgccctgttc accatcgcca agacctggaa ccagcccaag tgccccacca tgatcgattg
     4321 gatcaagaag atgtggcaca tctacaccat ggagtactac gccgccatca agaacgacga
     4381 gttcatcagc ttcgtgggca cctggatgaa gctggagacc atcatcctga gcaagctgag
     4441 ccaggagcag aagaccaagc accgcatctt cagcctgatc ggcggcaacg gcggcggcag
     4501 cggcggcggc agcctggaag ttctgttcca ggggcccgac tacaaagacc atgacggtga
     4561 ttataaagat catgacatcg attacaagga tgacgatgac aagtagacaa tgagatcaca
     4621 tggacacagg aaggggaata tcacactctg gggactgtgg tggggtcggg ggagggggga
     4681 gggatagcat tgggagatat acctaatgct agatgacaca ttagtgggtg cagcgcacca
     4741 gcatggcaca tgtatacata tgtaactaac ctgcacaatg tgcacatgta ccctaaaact
     4801 tagagtataa tggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     4861 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4921 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     4981 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     5041 gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     5101 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     5161 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg tcgatcgact
     5221 ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc
     5281 ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct
     5341 gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca
     5401 tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact
     5461 ttgcagtctg tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct
     5521 tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca
     5581 ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta
     5641 gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt
     5701 agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc
     5761 atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc
     5821 atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt
     5881 tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg
     5941 cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa
     6001 ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga
     6061 cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg
     6121 gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg
     6181 gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca
     6241 acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc
     6301 aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc
     6361 gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg
     6421 ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg
     6481 ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat
     6541 aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac
     6601 taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat
     6661 tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt
     6721 tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca
     6781 tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg
     6841 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     6901 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     6961 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
     7021 ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata
     7081 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag catatgctat
     7141 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     7201 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     7261 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     7321 cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag catatgatac
     7381 ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa gggggcgtga
     7441 attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa tttaaggagg
     7501 ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta atgttttcca
     7561 acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat gcccaattgc
     7621 cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca ccaacagcac
     7681 gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct tttaatacga
     7741 ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc atctccatca
     7801 cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc caccataggt
     7861 ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac cctgatattg
     7921 caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa aacctcagca
     7981 aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct tagcgggcca
     8041 ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg tgtaagacga
     8101 cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt cttactacct
     8161 ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc
     8221 ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt ggaacctcct
     8281 tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc accctgaccc
     8341 cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc
     8401 gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct
     8461 tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg
     8521 gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc
     8581 cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct
     8641 ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct
     8701 cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct
     8761 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     8821 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct
     8881 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     8941 cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct
     9001 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct
     9061 cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     9121 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     9181 gctcctgccc ctcctgcccc tcctscccct cctcctsctc ctgcccctcc tgcccctcct
     9241 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctsct
     9301 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc ccctcctcct
     9361 gctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcccgc tcctgctcct
     9421 gctcctgttc caccgtgggt ccctttgcag ccaatgcaac ttggacgttt ttggggtctc
     9481 cggacaccat ctctatgtct tggccctgat cctgagccgc ccggggctcc tggtcttccg
     9541 cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt tatcaccccc tcttctttga
     9601 ggtccactgc cgccggagcc ttctggtcca gatgtgtctc ccttctctcc taggccattt
     9661 ccaggtcctg tacctggccc ctcgtcagac atgattcaca ctaaaagaga tcaatagaca
     9721 tctttattag acgacgctca gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc
     9781 ccccccaccc tcatcccctt catggtcgct gtcagacaga tccaggtctg aaaattcccc
     9841 atcctccgaa ccatcctcgt cctcatcacc aattactcgc agcccggaaa actcccgctg
     9901 aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct cgtccccctt
     9961 tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt caaggtcacc
    10021 agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg aggatcagct
    10081 tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc
    10141 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
    10201 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    10261 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    10321 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    10381 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    10441 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    10501 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    10561 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    10621 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    10681 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    10741 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    10801 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    10861 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    10921 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    10981 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    11041 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    11101 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    11161 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    11221 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    11281 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    11341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    11401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    11461 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
    11521 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    11581 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    11641 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    11701 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11761 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11821 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11941 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttgaag ctgtccctga
    12001 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
    12061 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgaatt cgtcgacctc
    12121 gaaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
    12181 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
    12241 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
    12301 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
    12361 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
    12421 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
    12481 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
    12541 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
    12601 gttctcctct tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct
    12661 gaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
    12721 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
    12781 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
    12841 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
    12901 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
    12961 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
    13021 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
    13081 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
    13141 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    13201 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    13261 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
    13321 tgcatcgatg atatcagatc cccgggatgc agaaattgat gatctattaa acaataaaga
    13381 tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta
    13441 cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa
    13501 atgcctgctc tttactgaag gctctttact attgctttat gataatgttt catagttgga
    13561 tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat
    13621 ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt
    13681 tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc
    13741 ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa
    13801 gctggtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg
    13861 ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg
    13921 caattagcca tattagtcat tggttatata gcataaatca atattggcta ttggccattg
    13981 catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg
    14041 ccat
//
