LOCUS       sequence-422058-        9819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of base editor dCas12a-AID in mammalian cells for base 
            editing, along with non-fused EGFP fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, 
            Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao
            X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
  TITLE     Multiplexed base editing through Cas12a variant-mediated cytosine 
            and adenine base editors.
  JOURNAL   Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8.
  PUBMED    36323848
REFERENCE   2  (bases 1 to 9819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-2"; volume: "5(1):1163. doi"; pages: " 
            10.1038/s42003-022-04152-8"
COMMENT     Sequence Label: pCMV-dCas12a-AID
FEATURES             Location/Qualifiers
     source          1..9819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             409..1002
                     /codon_start=1
                     /gene="AICDA"
                     /product="human activation-induced cytidine deaminase 
                     (Dickerson et al., 2003)"
                     /label=AID
                     /translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
                     FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
                     SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
                     GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
     CDS             4741..4761
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4774..5022
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5035..5055
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5062..5115
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5116..5364
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5365..5385
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5386..5442
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5443..5691
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5692..5712
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5713..5766
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5767..6015
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6028..6048
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6058..6117
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             join(6118..6120,6121..6834)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6118..6120,6121..6831)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6160..6181)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6421..6440)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6768..6789
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6874..6891)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6880..7104
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7175..7191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7175..7191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7188..7210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7199..7215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7223..7229,7230..7247,7248..7253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7268..7289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7406..7423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7577..8165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7657..7676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8336..9127,9128..9196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8959..8978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9197..9301)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9380..9399)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9571..9819,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggatagcctc
      421 ttgatgaata gacgcaagtt cctgtatcag tttaaaaacg tgagatgggc aaaaggccga
      481 cgagagacat atctgtgcta tgtcgttaag cgcagagatt cagccaccag tttctctctc
      541 gacttcggct acctgcggaa caagaatggt tgccatgttg agctcctgtt cctgaggtat
      601 atcagcgact gggatttgga cccagggcgg tgctataggg tgacatggtt tacctcctgg
      661 tcaccttgtt atgactgcgc gcggcatgtt gccgattttc tgagagggaa ccctaacctg
      721 tctctgagga tcttcaccgc gcgactgtac ttctgtgagg accggaaagc cgaacccgag
      781 ggactgagac gcctccacag agcgggtgtg cagattgcca taatgacctt taaggactac
      841 ttctactgct ggaacacctt cgtcgaaaat cacgagcgga ctttcaaggc ttgggaagga
      901 ttgcatgaaa acagcgtcag gctttccagg cagcttcgcc gcattcttct cccgttgtac
      961 gaggttgatg acctcagaga tgcctttaga acactgggac tgagcggcag cgagactccc
     1021 gggacctcag agtccgccac acccgaaagt atgtcaaagc tggagaaatt caccaactgt
     1081 tatagcctgt ctaagaccct gcgcttcaag gcaatcccag tgggcaagac acaagagaac
     1141 attgacaaca aacggctcct ggtggaggat gagaagaggg ctgaagatta caagggcgtt
     1201 aagaagctgc tggataggta ctatctgtca ttcatcaacg atgtcctcca cagtatcaag
     1261 ctgaagaatc tgaacaatta catttctctg ttccggaaga agacacggac cgagaaggag
     1321 aacaaagagc tggagaatct ggagatcaac ctgaggaaag aaatagctaa ggctttcaaa
     1381 gggaacgagg gttacaagtc cctgttcaag aaagacatta tcgagactat tctgcctgag
     1441 ttcctggacg ataaagatga gatcgccctc gtcaattcct tcaatgggtt taccacagcc
     1501 tttaccggct tcttcgacaa tagagagaat atgttctctg aagaggccaa atccactagc
     1561 atcgcctttc gctgcataaa cgagaacctg actaggtaca tcagcaatat ggacatcttt
     1621 gagaaagtcg atgccatatt cgacaaacat gaggtgcagg agattaagga gaagatcctg
     1681 aactcagatt acgatgtcga agatttcttc gagggagagt tcttcaactt cgtgctcaca
     1741 caagagggca ttgatgtgta caatgcaatc attggagggt tcgtgacaga gagtggcgag
     1801 aagataaagg gcctgaacga gtatatcaac ctctacaacc agaaaaccaa gcagaaactg
     1861 cctaagttca agccactgta caaacaagtg ctctcagata gggaaagcct gagcttctac
     1921 ggtgaagggt atacatcaga tgaagaagtg ctcgaagtgt tccgcaacac cctcaataag
     1981 aacagtgaaa tcttctcttc aatcaagaag ctggagaaac tgttcaagaa tttcgatgag
     2041 tactcctctg ccggaatctt tgtgaagaat ggccctgcaa tatccactat tagcaaagac
     2101 atctttggcg agtggaacgt tatcagggat aagtggaatg ccgagtacga tgatattcat
     2161 ctcaagaaga aagccgtggt tacagagaaa tacgaggatg atagacgcaa gagctttaag
     2221 aagattggta gcttctctct cgaacagctg caggagtacg ccgacgctga cctgtcagtc
     2281 gtggagaaac tcaaggagat cataatccag aaggtggatg aaatctacaa agtgtatgga
     2341 agctctgaga aactcttcga tgcagacttt gttctggaga agagtctgaa gaagaacgac
     2401 gcagtggttg ctatcatgaa ggacctgctg gattctgtta agtctttcga gaattacatt
     2461 aaggcattct ttggtgaagg gaaggagaca aatagggacg agagcttcta tggcgacttt
     2521 gttctggcct acgacatcct cctcaaggtt gaccacatct atgacgctat acggaattac
     2581 gttacccaga agccctatag caaagacaag ttcaagctgt atttccagaa tccacagttt
     2641 atgggtgggt gggataaaga caaagaaaca gattacaggg ccactatcct gcggtacggc
     2701 agcaaatact atctggctat catggataag aagtacgcca aatgcctcca gaagatcgac
     2761 aaggacgacg tgaacggtaa ctacgagaag atcaattaca agctcctgcc aggacctaac
     2821 aagatgctgc ccaaggtgtt cttctccaag aaatggatgg cctactataa cccaagcgag
     2881 gacattcaga agatatacaa gaatgggaca ttcaagaagg gcgatatgtt caacctcaac
     2941 gactgccaca agctgattga tttcttcaag gatagcattt ctcgctatcc caagtggtct
     3001 aatgcatacg atttcaactt cagcgagact gagaagtaca aagacatcgc tggcttctac
     3061 cgggaggtgg aagagcaagg ctataaggtg tcattcgaat ccgcttctaa gaaggaagtg
     3121 gataagctcg tggaagaggg taagctgtac atgttccaga tatacaacaa agacttcagc
     3181 gataagagcc acggcactcc aaacctccat actatgtatt tcaagctgct gtttgacgag
     3241 aacaaccacg gacagattag gctgtcagga ggcgcagaac tcttcatgcg cagagcttca
     3301 ctgaagaagg aggaactcgt tgtccaccca gccaatagcc ctatagccaa taagaatcca
     3361 gacaatccta agaaaaccac tactctgtct tacgatgtgt ataaggataa gagattctct
     3421 gaagatcagt acgaactgca catacccatt gccattaaca agtgccctaa gaacatcttc
     3481 aagattaaca cagaggttag agtgctcctg aaacacgacg ataaccctta tgttataggc
     3541 attgctcgcg gagagagaaa cctgctgtac atcgtcgtgg tggacggcaa aggcaacatc
     3601 gtggaacagt acagtctcaa tgaaatcatt aacaatttca acggaatccg cattaagacc
     3661 gactaccatt ctctcctcga caagaaggag aaagaaaggt tcgaagcaag acagaattgg
     3721 acaagtatag agaatatcaa agaactgaag gctgggtaca tctctcaggt tgtgcacaag
     3781 atatgtgagc tggtggagaa gtacgacgct gttatcgccc tcgcggacct gaatagcggc
     3841 ttcaagaact ccagggtgaa ggtggagaag caggtgtatc agaagttcga gaagatgctg
     3901 atcgacaagc tcaactatat ggtggacaag aaatccaatc cttgcgctac tggtggagcc
     3961 ctgaagggct atcaaatcac caataagttc gaatctttca agtctatgag cacccagaat
     4021 ggcttcatct tctacatacc cgcatggctg acatccaaga ttgatccctc taccggattt
     4081 gttaatctgc tcaagactaa gtacacctct attgctgact caaagaagtt catatcatca
     4141 tttgaccgca tcatgtacgt gccagaagag gacctgttcg agtttgccct ggattacaag
     4201 aatttctctc ggactgacgc cgactacatc aagaagtgga agctctactc ttatggtaat
     4261 cggattcgca tattccgcaa tcccaagaag aataacgtgt tcgattggga ggaagtttgc
     4321 ctcaccagcg cttacaagga gctgttcaat aagtatggga ttaactacca gcagggcgac
     4381 ataagagccc tgctgtgcga acaatctgat aaggcattct attcctcttt catggcactg
     4441 atgtcactga tgctgcaaat gcgcaattcc atcaccggaa gaacagacgt ggcctttctg
     4501 atctctcctg tcaagaactc agatggcatc ttctacgatt cccgcaacta tgaagcacag
     4561 gagaatgcta tcctgcctaa gaatgccgat gcaaatggag cctataacat cgccagaaag
     4621 gtcctctggg ccataggaca attcaagaaa gctgaagatg agaagctgga caaggtgaag
     4681 atcgccattt caaacaaaga gtggctcgaa tatgctcaga cctcagtgaa gcatggatca
     4741 cccaagaaga aacggaaagt gtctggtggt tctactaatc tgtcagatat tattgaaaag
     4801 gagaccggta agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa
     4861 gaagtcattg ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc
     4921 accgacgaga atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg
     4981 gtcatacagg atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag
     5041 aagaagagga aagtcgggcc cgagggcaga ggaagtctgc taacatgcgg tgacgtcgag
     5101 gagaatcctg gcccaactaa tctgtcagat attattgaaa aggagaccgg taagcaactg
     5161 gttatccagg aatccatcct catgctccca gaggaggtgg aagaagtcat tgggaacaag
     5221 ccggaaagcg atatactcgt gcacaccgcc tacgacgaga gcaccgacga gaatgtcatg
     5281 cttctgacta gcgacgcccc tgaatacaag ccttgggctc tggtcataca ggatagcaac
     5341 ggtgagaaca agattaagat gctccccaag aagaagagga aagtcgctac taacttcagc
     5401 ctgctgaagc aggctggaga cgtggaggag aaccctggac ctaccaacct gtccgatatc
     5461 attgagaaag agaccggcaa acagctggtg atccaggaga gcatcctgat gctgcccgaa
     5521 gaggtggagg aagtgatcgg caacaagccc gagtccgaca tcctggtgca cacagcctat
     5581 gatgaatcca ccgacgagaa cgtgatgctg ctgacctccg atgctcccga gtataaaccc
     5641 tgggcactgg tgatccagga ctctaatgga gagaacaaga tcaagatgct gcccaagaag
     5701 aagaggaaag tcgaaggacg gggctccctc ctgacctgtg gcgatgtgga agagaaccct
     5761 ggccccacaa acctcagtga cattatcgag aaggaaacag gaaaacagct cgtcattcaa
     5821 gaatctattc ttatgttgcc tgaggaagtc gaagaggtta ttggcaataa acctgaatct
     5881 gatattcttg tccataccgc ttacgatgag tccacagatg aaaatgttat gctgctcaca
     5941 tctgacgcac cagagtacaa accatgggcg ctcgttattc aagattccaa cggcgaaaac
     6001 aaaatcaaaa tgctttctgg tggttctcct aaaaaaaaaa gaaaggtggg ctcgggccag
     6061 tgtactaatt atgctctctt gaaattggct ggagatgttg agagcaaccc aggtcccgtg
     6121 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     6181 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     6241 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     6301 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     6361 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     6421 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     6481 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     6541 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     6601 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     6661 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     6721 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     6781 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaatcatc
     6841 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     6901 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6961 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     7021 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7081 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     7141 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7201 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     7261 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7321 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcggga agaggcggtt
     7381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8641 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8701 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8761 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8821 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8881 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8941 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9001 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9061 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9121 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9181 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9241 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9301 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa tctcccgatc
     9361 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     9421 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     9481 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     9541 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     9601 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     9661 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     9721 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     9781 tacggtaaac tgcccacttg gcagtacatc aagtgtatc
//
