LOCUS       sequence-422059-        9936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of base editor dCas12a-BE in mammalian cells for base 
            editing, along with non-fused EGFP fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, 
            Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao
            X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
  TITLE     Multiplexed base editing through Cas12a variant-mediated cytosine 
            and adenine base editors.
  JOURNAL   Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8.
  PUBMED    36323848
REFERENCE   2  (bases 1 to 9936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-2"; volume: "5(1):1163. doi"; pages: " 
            10.1038/s42003-022-04152-8"
COMMENT     Sequence Label: pCMV-dCas12a-BE
FEATURES             Location/Qualifiers
     source          1..9936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             412..432
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             433..1119
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             4858..4878
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4891..5139
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5152..5172
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5179..5232
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5233..5481
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5482..5502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5503..5559
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5560..5808
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5809..5829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5830..5883
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5884..6132
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6145..6165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6175..6234
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             join(6235..6237,6238..6951)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6235..6237,6238..6948)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6277..6298)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6538..6557)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6885..6906
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6991..7008)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6997..7221
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7292..7308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7292..7308)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7305..7327)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7316..7332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7340..7346,7347..7364,7365..7370))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7385..7406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7523..7540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7694..8282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7774..7793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8453..9244,9245..9313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9076..9095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9314..9418)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9497..9516)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9688..9936,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gcccaagaag
      421 aagaggaaag tcatgagctc agagactggc ccagtggctg tggaccccac attgagacgg
      481 cggatcgagc cccatgagtt tgaggtattc ttcgatccga gagagctccg caaggagacc
      541 tgcctgcttt acgaaattaa ttgggggggc cggcactcca tttggcgaca tacatcacag
      601 aacactaaca agcacgtcga agtcaacttc atcgagaagt tcacgacaga aagatatttc
      661 tgtccgaaca caaggtgcag cattacctgg tttctcagct ggagcccatg cggcgaatgt
      721 agtagggcca tcactgaatt cctgtcaagg tatccccacg tcactctgtt tatttacatc
      781 gcaaggctgt accaccacgc tgacccccgc aatcgacaag gcctgcggga tttgatctct
      841 tcaggtgtga ctatccaaat tatgactgag caggagtcag gatactgctg gagaaacttt
      901 gtgaattata gcccgagtaa tgaagcccac tggcctaggt atccccatct gtgggtacga
      961 ctgtacgttc ttgaactgta ctgcatcata ctgggcctgc ctccttgtct caacattctg
     1021 agaaggaagc agccacagct gacattcttt accatcgctc ttcagtcttg tcattaccag
     1081 cgactgcccc cacacattct ctgggccacc gggttgaaaa gcggcagcga gactcccggg
     1141 acctcagagt ccgccacacc cgaaagtatg tcaaagctgg agaaattcac caactgttat
     1201 agcctgtcta agaccctgcg cttcaaggca atcccagtgg gcaagacaca agagaacatt
     1261 gacaacaaac ggctcctggt ggaggatgag aagagggctg aagattacaa gggcgttaag
     1321 aagctgctgg ataggtacta tctgtcattc atcaacgatg tcctccacag tatcaagctg
     1381 aagaatctga acaattacat ttctctgttc cggaagaaga cacggaccga gaaggagaac
     1441 aaagagctgg agaatctgga gatcaacctg aggaaagaaa tagctaaggc tttcaaaggg
     1501 aacgagggtt acaagtccct gttcaagaaa gacattatcg agactattct gcctgagttc
     1561 ctggacgata aagatgagat cgccctcgtc aattccttca atgggtttac cacagccttt
     1621 accggcttct tcgacaatag agagaatatg ttctctgaag aggccaaatc cactagcatc
     1681 gcctttcgct gcataaacga gaacctgact aggtacatca gcaatatgga catctttgag
     1741 aaagtcgatg ccatattcga caaacatgag gtgcaggaga ttaaggagaa gatcctgaac
     1801 tcagattacg atgtcgaaga tttcttcgag ggagagttct tcaacttcgt gctcacacaa
     1861 gagggcattg atgtgtacaa tgcaatcatt ggagggttcg tgacagagag tggcgagaag
     1921 ataaagggcc tgaacgagta tatcaacctc tacaaccaga aaaccaagca gaaactgcct
     1981 aagttcaagc cactgtacaa acaagtgctc tcagataggg aaagcctgag cttctacggt
     2041 gaagggtata catcagatga agaagtgctc gaagtgttcc gcaacaccct caataagaac
     2101 agtgaaatct tctcttcaat caagaagctg gagaaactgt tcaagaattt cgatgagtac
     2161 tcctctgccg gaatctttgt gaagaatggc cctgcaatat ccactattag caaagacatc
     2221 tttggcgagt ggaacgttat cagggataag tggaatgccg agtacgatga tattcatctc
     2281 aagaagaaag ccgtggttac agagaaatac gaggatgata gacgcaagag ctttaagaag
     2341 attggtagct tctctctcga acagctgcag gagtacgccg acgctgacct gtcagtcgtg
     2401 gagaaactca aggagatcat aatccagaag gtggatgaaa tctacaaagt gtatggaagc
     2461 tctgagaaac tcttcgatgc agactttgtt ctggagaaga gtctgaagaa gaacgacgca
     2521 gtggttgcta tcatgaagga cctgctggat tctgttaagt ctttcgagaa ttacattaag
     2581 gcattctttg gtgaagggaa ggagacaaat agggacgaga gcttctatgg cgactttgtt
     2641 ctggcctacg acatcctcct caaggttgac cacatctatg acgctatacg gaattacgtt
     2701 acccagaagc cctatagcaa agacaagttc aagctgtatt tccagaatcc acagtttatg
     2761 ggtgggtggg ataaagacaa agaaacagat tacagggcca ctatcctgcg gtacggcagc
     2821 aaatactatc tggctatcat ggataagaag tacgccaaat gcctccagaa gatcgacaag
     2881 gacgacgtga acggtaacta cgagaagatc aattacaagc tcctgccagg acctaacaag
     2941 atgctgccca aggtgttctt ctccaagaaa tggatggcct actataaccc aagcgaggac
     3001 attcagaaga tatacaagaa tgggacattc aagaagggcg atatgttcaa cctcaacgac
     3061 tgccacaagc tgattgattt cttcaaggat agcatttctc gctatcccaa gtggtctaat
     3121 gcatacgatt tcaacttcag cgagactgag aagtacaaag acatcgctgg cttctaccgg
     3181 gaggtggaag agcaaggcta taaggtgtca ttcgaatccg cttctaagaa ggaagtggat
     3241 aagctcgtgg aagagggtaa gctgtacatg ttccagatat acaacaaaga cttcagcgat
     3301 aagagccacg gcactccaaa cctccatact atgtatttca agctgctgtt tgacgagaac
     3361 aaccacggac agattaggct gtcaggaggc gcagaactct tcatgcgcag agcttcactg
     3421 aagaaggagg aactcgttgt ccacccagcc aatagcccta tagccaataa gaatccagac
     3481 aatcctaaga aaaccactac tctgtcttac gatgtgtata aggataagag attctctgaa
     3541 gatcagtacg aactgcacat acccattgcc attaacaagt gccctaagaa catcttcaag
     3601 attaacacag aggttagagt gctcctgaaa cacgacgata acccttatgt tataggcatt
     3661 gctcgcggag agagaaacct gctgtacatc gtcgtggtgg acggcaaagg caacatcgtg
     3721 gaacagtaca gtctcaatga aatcattaac aatttcaacg gaatccgcat taagaccgac
     3781 taccattctc tcctcgacaa gaaggagaaa gaaaggttcg aagcaagaca gaattggaca
     3841 agtatagaga atatcaaaga actgaaggct gggtacatct ctcaggttgt gcacaagata
     3901 tgtgagctgg tggagaagta cgacgctgtt atcgccctcg cggacctgaa tagcggcttc
     3961 aagaactcca gggtgaaggt ggagaagcag gtgtatcaga agttcgagaa gatgctgatc
     4021 gacaagctca actatatggt ggacaagaaa tccaatcctt gcgctactgg tggagccctg
     4081 aagggctatc aaatcaccaa taagttcgaa tctttcaagt ctatgagcac ccagaatggc
     4141 ttcatcttct acatacccgc atggctgaca tccaagattg atccctctac cggatttgtt
     4201 aatctgctca agactaagta cacctctatt gctgactcaa agaagttcat atcatcattt
     4261 gaccgcatca tgtacgtgcc agaagaggac ctgttcgagt ttgccctgga ttacaagaat
     4321 ttctctcgga ctgacgccga ctacatcaag aagtggaagc tctactctta tggtaatcgg
     4381 attcgcatat tccgcaatcc caagaagaat aacgtgttcg attgggagga agtttgcctc
     4441 accagcgctt acaaggagct gttcaataag tatgggatta actaccagca gggcgacata
     4501 agagccctgc tgtgcgaaca atctgataag gcattctatt cctctttcat ggcactgatg
     4561 tcactgatgc tgcaaatgcg caattccatc accggaagaa cagacgtggc ctttctgatc
     4621 tctcctgtca agaactcaga tggcatcttc tacgattccc gcaactatga agcacaggag
     4681 aatgctatcc tgcctaagaa tgccgatgca aatggagcct ataacatcgc cagaaaggtc
     4741 ctctgggcca taggacaatt caagaaagct gaagatgaga agctggacaa ggtgaagatc
     4801 gccatttcaa acaaagagtg gctcgaatat gctcagacct cagtgaagca tggatcaccc
     4861 aagaagaaac ggaaagtgtc tggtggttct actaatctgt cagatattat tgaaaaggag
     4921 accggtaagc aactggttat ccaggaatcc atcctcatgc tcccagagga ggtggaagaa
     4981 gtcattggga acaagccgga aagcgatata ctcgtgcaca ccgcctacga cgagagcacc
     5041 gacgagaatg tcatgcttct gactagcgac gcccctgaat acaagccttg ggctctggtc
     5101 atacaggata gcaacggtga gaacaagatt aagatgctct ctggtggttc tcccaagaag
     5161 aagaggaaag tcgggcccga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     5221 aatcctggcc caactaatct gtcagatatt attgaaaagg agaccggtaa gcaactggtt
     5281 atccaggaat ccatcctcat gctcccagag gaggtggaag aagtcattgg gaacaagccg
     5341 gaaagcgata tactcgtgca caccgcctac gacgagagca ccgacgagaa tgtcatgctt
     5401 ctgactagcg acgcccctga atacaagcct tgggctctgg tcatacagga tagcaacggt
     5461 gagaacaaga ttaagatgct ccccaagaag aagaggaaag tcgctactaa cttcagcctg
     5521 ctgaagcagg ctggagacgt ggaggagaac cctggaccta ccaacctgtc cgatatcatt
     5581 gagaaagaga ccggcaaaca gctggtgatc caggagagca tcctgatgct gcccgaagag
     5641 gtggaggaag tgatcggcaa caagcccgag tccgacatcc tggtgcacac agcctatgat
     5701 gaatccaccg acgagaacgt gatgctgctg acctccgatg ctcccgagta taaaccctgg
     5761 gcactggtga tccaggactc taatggagag aacaagatca agatgctgcc caagaagaag
     5821 aggaaagtcg aaggacgggg ctccctcctg acctgtggcg atgtggaaga gaaccccggc
     5881 cccacaaacc tcagtgacat tatcgagaag gaaacaggaa aacagctcgt cattcaagaa
     5941 tctattctta tgttgcctga ggaagtcgaa gaggttattg gcaataaacc tgaatctgat
     6001 attcttgtcc ataccgctta cgatgagtcc acagatgaaa atgttatgct gctcacatct
     6061 gacgcaccag agtacaaacc atgggcgctc gttattcaag attccaacgg cgaaaacaaa
     6121 atcaaaatgc tttctggtgg ttctcctaaa aaaaaaagaa aggtgggctc gggccagtgt
     6181 actaattatg ctctcttgaa attggctgga gatgttgaga gcaacccagg tcccgtgagc
     6241 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     6301 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     6361 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     6421 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     6481 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     6541 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     6601 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     6661 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     6721 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     6781 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     6841 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     6901 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aatcatcacc
     6961 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     7021 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     7081 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     7141 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     7201 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt
     7261 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7321 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg
     7381 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7441 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7501 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8101 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8341 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8401 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8461 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8521 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8581 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8641 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8701 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8761 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8821 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8881 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8941 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9001 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9061 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9121 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9181 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9241 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9301 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9361 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9421 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc
     9481 tagggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     9541 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     9601 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     9661 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     9721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     9781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     9841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     9901 ggtaaactgc ccacttggca gtacatcaag tgtatc
//
