LOCUS       sequence-422060-        9849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of base editor dCas12a-CDA1 in mammalian cells for base 
            editing, along with non-fused EGFP fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, 
            Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao
            X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
  TITLE     Multiplexed base editing through Cas12a variant-mediated cytosine 
            and adenine base editors.
  JOURNAL   Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8.
  PUBMED    36323848
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-2"; volume: "5(1):1163. doi"; pages: " 
            10.1038/s42003-022-04152-8"
COMMENT     Sequence Label: pCMV-dCas12a-CDA1
FEATURES             Location/Qualifiers
     source          1..9849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4771..4791
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4804..5052
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5065..5085
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5092..5145
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5146..5394
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5395..5415
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5416..5472
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5473..5721
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5722..5742
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5743..5796
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5797..6045
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6058..6078
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6088..6147
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             join(6148..6150,6151..6864)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6148..6150,6151..6861)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6190..6211)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6451..6470)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6798..6819
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6904..6921)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6910..7134
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7205..7221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7205..7221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7218..7240)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7229..7245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7253..7259,7260..7277,7278..7283))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7298..7319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7436..7453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7607..8195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7687..7706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8366..9157,9158..9226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8989..9008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9227..9331)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9410..9429)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9601..9849,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gacagacgcc
      421 gagtacgtgc gcattcatga gaaactggat atttacacct tcaagaagca gttcttcaac
      481 aacaagaaat ctgtgtcaca ccgctgctac gtgctgtttg agttgaagcg aaggggcgaa
      541 agaagggctt gcttttgggg ctatgccgtc aacaagcccc aaagtggcac cgagagagga
      601 atacacgctg agatattcag tatccgaaag gtggaagagt atcttcggga taatcctggg
      661 cagtttacga tcaactggta ttccagctgg agtccttgcg ctgattgtgc cgagaaaatt
      721 ctggaatggt ataatcagga acttcgggga aacgggcaca cattgaaaat ctgggcctgc
      781 aagctgtact acgagaagaa tgcccggaac cagataggac tctggaatct gagggacaat
      841 ggtgtaggcc tgaacgtgat ggtttccgag cactatcagt gttgtcggaa gattttcatc
      901 caaagctctc ataaccagct caatgaaaac cgctggttgg agaaaacact gaaacgtgcg
      961 gagaagtgga gatccgagct gagcatcatg atccaggtca agattctgca taccactaag
     1021 tctccagccg ttagcggcag cgagactccc gggacctcag agtccgccac acccgaaagt
     1081 atgtcaaagc tggagaaatt caccaactgt tatagcctgt ctaagaccct gcgcttcaag
     1141 gcaatcccag tgggcaagac acaagagaac attgacaaca aacggctcct ggtggaggat
     1201 gagaagaggg ctgaagatta caagggcgtt aagaagctgc tggataggta ctatctgtca
     1261 ttcatcaacg atgtcctcca cagtatcaag ctgaagaatc tgaacaatta catttctctg
     1321 ttccggaaga agacacggac cgagaaggag aacaaagagc tggagaatct ggagatcaac
     1381 ctgaggaaag aaatagctaa ggctttcaaa gggaacgagg gttacaagtc cctgttcaag
     1441 aaagacatta tcgagactat tctgcctgag ttcctggacg ataaagatga gatcgccctc
     1501 gtcaattcct tcaatgggtt taccacagcc tttaccggct tcttcgacaa tagagagaat
     1561 atgttctctg aagaggccaa atccactagc atcgcctttc gctgcataaa cgagaacctg
     1621 actaggtaca tcagcaatat ggacatcttt gagaaagtcg atgccatatt cgacaaacat
     1681 gaggtgcagg agattaagga gaagatcctg aactcagatt acgatgtcga agatttcttc
     1741 gagggagagt tcttcaactt cgtgctcaca caagagggca ttgatgtgta caatgcaatc
     1801 attggagggt tcgtgacaga gagtggcgag aagataaagg gcctgaacga gtatatcaac
     1861 ctctacaacc agaaaaccaa gcagaaactg cctaagttca agccactgta caaacaagtg
     1921 ctctcagata gggaaagcct gagcttctac ggtgaagggt atacatcaga tgaagaagtg
     1981 ctcgaagtgt tccgcaacac cctcaataag aacagtgaaa tcttctcttc aatcaagaag
     2041 ctggagaaac tgttcaagaa tttcgatgag tactcctctg ccggaatctt tgtgaagaat
     2101 ggccctgcaa tatccactat tagcaaagac atctttggcg agtggaacgt tatcagggat
     2161 aagtggaatg ccgagtacga tgatattcat ctcaagaaga aagccgtggt tacagagaaa
     2221 tacgaggatg atagacgcaa gagctttaag aagattggta gcttctctct cgaacagctg
     2281 caggagtacg ccgacgctga cctgtcagtc gtggagaaac tcaaggagat cataatccag
     2341 aaggtggatg aaatctacaa agtgtatgga agctctgaga aactcttcga tgcagacttt
     2401 gttctggaga agagtctgaa gaagaacgac gcagtggttg ctatcatgaa ggacctgctg
     2461 gattctgtta agtctttcga gaattacatt aaggcattct ttggtgaagg gaaggagaca
     2521 aatagggacg agagcttcta tggcgacttt gttctggcct acgacatcct cctcaaggtt
     2581 gaccacatct atgacgctat acggaattac gttacccaga agccctatag caaagacaag
     2641 ttcaagctgt atttccagaa tccacagttt atgggtgggt gggataaaga caaagaaaca
     2701 gattacaggg ccactatcct gcggtacggc agcaaatact atctggctat catggataag
     2761 aagtacgcca aatgcctcca gaagatcgac aaggacgacg tgaacggtaa ctacgagaag
     2821 atcaattaca agctcctgcc aggacctaac aagatgctgc ccaaggtgtt cttctccaag
     2881 aaatggatgg cctactataa cccaagcgag gacattcaga agatatacaa gaatgggaca
     2941 ttcaagaagg gcgatatgtt caacctcaac gactgccaca agctgattga tttcttcaag
     3001 gatagcattt ctcgctatcc caagtggtct aatgcatacg atttcaactt cagcgagact
     3061 gagaagtaca aagacatcgc tggcttctac cgggaggtgg aagagcaagg ctataaggtg
     3121 tcattcgaat ccgcttctaa gaaggaagtg gataagctcg tggaagaggg taagctgtac
     3181 atgttccaga tatacaacaa agacttcagc gataagagcc acggcactcc aaacctccat
     3241 actatgtatt tcaagctgct gtttgacgag aacaaccacg gacagattag gctgtcagga
     3301 ggcgcagaac tcttcatgcg cagagcttca ctgaagaagg aggaactcgt tgtccaccca
     3361 gccaatagcc ctatagccaa taagaatcca gacaatccta agaaaaccac tactctgtct
     3421 tacgatgtgt ataaggataa gagattctct gaagatcagt acgaactgca catacccatt
     3481 gccattaaca agtgccctaa gaacatcttc aagattaaca cagaggttag agtgctcctg
     3541 aaacacgacg ataaccctta tgttataggc attgctcgcg gagagagaaa cctgctgtac
     3601 atcgtcgtgg tggacggcaa aggcaacatc gtggaacagt acagtctcaa tgaaatcatt
     3661 aacaatttca acggaatccg cattaagacc gactaccatt ctctcctcga caagaaggag
     3721 aaagaaaggt tcgaagcaag acagaattgg acaagtatag agaatatcaa agaactgaag
     3781 gctgggtaca tctctcaggt tgtgcacaag atatgtgagc tggtggagaa gtacgacgct
     3841 gttatcgccc tcgcggacct gaatagcggc ttcaagaact ccagggtgaa ggtggagaag
     3901 caggtgtatc agaagttcga gaagatgctg atcgacaagc tcaactatat ggtggacaag
     3961 aaatccaatc cttgcgctac tggtggagcc ctgaagggct atcaaatcac caataagttc
     4021 gaatctttca agtctatgag cacccagaat ggcttcatct tctacatacc cgcatggctg
     4081 acatccaaga ttgatccctc taccggattt gttaatctgc tcaagactaa gtacacctct
     4141 attgctgact caaagaagtt catatcatca tttgaccgca tcatgtacgt gccagaagag
     4201 gacctgttcg agtttgccct ggattacaag aatttctctc ggactgacgc cgactacatc
     4261 aagaagtgga agctctactc ttatggtaat cggattcgca tattccgcaa tcccaagaag
     4321 aataacgtgt tcgattggga ggaagtttgc ctcaccagcg cttacaagga gctgttcaat
     4381 aagtatggga ttaactacca gcagggcgac ataagagccc tgctgtgcga acaatctgat
     4441 aaggcattct attcctcttt catggcactg atgtcactga tgctgcaaat gcgcaattcc
     4501 atcaccggaa gaacagacgt ggcctttctg atctctcctg tcaagaactc agatggcatc
     4561 ttctacgatt cccgcaacta tgaagcacag gagaatgcta tcctgcctaa gaatgccgat
     4621 gcaaatggag cctataacat cgccagaaag gtcctctggg ccataggaca attcaagaaa
     4681 gctgaagatg agaagctgga caaggtgaag atcgccattt caaacaaaga gtggctcgaa
     4741 tatgctcaga cctcagtgaa gcatggatca cccaagaaga aacggaaagt gtctggtggt
     4801 tctactaatc tgtcagatat tattgaaaag gagaccggta agcaactggt tatccaggaa
     4861 tccatcctca tgctcccaga ggaggtggaa gaagtcattg ggaacaagcc ggaaagcgat
     4921 atactcgtgc acaccgccta cgacgagagc accgacgaga atgtcatgct tctgactagc
     4981 gacgcccctg aatacaagcc ttgggctctg gtcatacagg atagcaacgg tgagaacaag
     5041 attaagatgc tctctggtgg ttctcccaag aagaagagga aagtcgggcc cgagggcaga
     5101 ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaactaa tctgtcagat
     5161 attattgaaa aggagaccgg taagcaactg gttatccagg aatccatcct catgctccca
     5221 gaggaggtgg aagaagtcat tgggaacaag ccggaaagcg atatactcgt gcacaccgcc
     5281 tacgacgaga gcaccgacga gaatgtcatg cttctgacta gcgacgcccc tgaatacaag
     5341 ccttgggctc tggtcataca ggatagcaac ggtgagaaca agattaagat gctccccaag
     5401 aagaagagga aagtcgctac taacttcagc ctgctgaagc aggctggaga cgtggaggag
     5461 aaccctggac ctaccaacct gtccgatatc attgagaaag agaccggcaa acagctggtg
     5521 atccaggaga gcatcctgat gctgcccgaa gaggtggagg aagtgatcgg caacaagccc
     5581 gagtccgaca tcctggtgca cacagcctat gatgaatcca ccgacgagaa cgtgatgctg
     5641 ctgacctccg atgctcccga gtataaaccc tgggcactgg tgatccagga ctctaatgga
     5701 gagaacaaga tcaagatgct gcccaagaag aagaggaaag tcgaaggacg gggctccctc
     5761 ctgacctgtg gcgatgtgga agagaacccc ggccccacaa acctcagtga cattatcgag
     5821 aaggaaacag gaaaacagct cgtcattcaa gaatctattc ttatgttgcc tgaggaagtc
     5881 gaagaggtta ttggcaataa acctgaatct gatattcttg tccataccgc ttacgatgag
     5941 tccacagatg aaaatgttat gctgctcaca tctgacgcac cagagtacaa accatgggcg
     6001 ctcgttattc aagattccaa cggcgaaaac aaaatcaaaa tgctttctgg tggttctcct
     6061 aaaaaaaaaa gaaaggtggg ctcgggccag tgtactaatt atgctctctt gaaattggct
     6121 ggagatgttg agagcaaccc aggtcccgtg agcaagggcg aggagctgtt caccggggtg
     6181 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     6241 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     6301 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     6361 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     6421 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     6481 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     6541 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     6601 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     6661 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     6721 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     6781 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     6841 ggcatggacg agctgtacaa gtaaatcatc accatcacca ttgagtttaa acccgctgat
     6901 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6961 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7021 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     7081 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     7141 aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat
     7201 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7261 gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa
     7321 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7381 gaatcggcca acgcgcggga agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8041 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8401 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8461 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8521 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8581 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8641 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8701 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8761 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8821 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8881 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8941 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9001 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9061 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9121 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9181 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9241 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9301 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9361 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
     9421 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     9481 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     9541 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     9601 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     9661 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     9721 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     9781 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     9841 aagtgtatc
//
