LOCUS       sequence-422061-        9825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of base editor dCas12a-eAID in mammalian cells for base 
            editing, along with non-fused EGFP fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, 
            Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao
            X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
  TITLE     Multiplexed base editing through Cas12a variant-mediated cytosine 
            and adenine base editors.
  JOURNAL   Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8.
  PUBMED    36323848
REFERENCE   2  (bases 1 to 9825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-2"; volume: "5(1):1163. doi"; pages: " 
            10.1038/s42003-022-04152-8"
COMMENT     Sequence Label: pCMV-dCas12a-eAID
FEATURES             Location/Qualifiers
     source          1..9825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4747..4767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4780..5028
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5041..5061
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5068..5121
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5122..5370
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5371..5391
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5392..5448
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5449..5697
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5698..5718
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5719..5772
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5773..6021
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6034..6054
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6064..6123
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             join(6124..6126,6127..6840)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6124..6126,6127..6837)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6166..6187)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6427..6446)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6774..6795
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6880..6897)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6886..7110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7181..7197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7181..7197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7194..7216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7205..7221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7229..7235,7236..7253,7254..7259))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7274..7295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7412..7429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7583..8171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7663..7682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8342..9133,9134..9202))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8965..8984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9203..9307)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9386..9405)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9577..9825,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgattc tctgctgatg
      481 aaccggagag agttcctgta tcagtttaag aatgtgaggt gggcaaaggg caggcgcgag
      541 acctacctgt gctatgtggt gaagcggaga gatagcgcca caagcttctc cctggacttt
      601 ggctacctgc ggaacaagaa tggctgccac gtggagctgc tgttcctgag atacatctcc
      661 gactgggatc tggaccccgg caggtgttat cgcgtgacct ggttcatctc ttggagccct
      721 tgctacgatt gtgcccggca cgtggccgac tttctgagag gcaacccaaa tctgtccctg
      781 aggatcttca ccgcccgcct gtatttttgc gaggatagga aggcagagcc agagggactg
      841 aggcgcctgc acagggccgg cgtgcagatc gccatcatga cattcaagga ctacttttat
      901 tgttggaaca ccttcgtgga gaatcacgga cggaccttca aggcatggga gggactgcac
      961 gagaactccg tgcggctgtc tagacagctg cggagaatcc tgctgcccag cggcagcgag
     1021 actcccggga cctcagagtc cgccacaccc gaaagtatgt caaagctgga gaaattcacc
     1081 aactgttata gcctgtctaa gaccctgcgc ttcaaggcaa tcccagtggg caagacacaa
     1141 gagaacattg acaacaaacg gctcctggtg gaggatgaga agagggctga agattacaag
     1201 ggcgttaaga agctgctgga taggtactat ctgtcattca tcaacgatgt cctccacagt
     1261 atcaagctga agaatctgaa caattacatt tctctgttcc ggaagaagac acggaccgag
     1321 aaggagaaca aagagctgga gaatctggag atcaacctga ggaaagaaat agctaaggct
     1381 ttcaaaggga acgagggtta caagtccctg ttcaagaaag acattatcga gactattctg
     1441 cctgagttcc tggacgataa agatgagatc gccctcgtca attccttcaa tgggtttacc
     1501 acagccttta ccggcttctt cgacaataga gagaatatgt tctctgaaga ggccaaatcc
     1561 actagcatcg cctttcgctg cataaacgag aacctgacta ggtacatcag caatatggac
     1621 atctttgaga aagtcgatgc catattcgac aaacatgagg tgcaggagat taaggagaag
     1681 atcctgaact cagattacga tgtcgaagat ttcttcgagg gagagttctt caacttcgtg
     1741 ctcacacaag agggcattga tgtgtacaat gcaatcattg gagggttcgt gacagagagt
     1801 ggcgagaaga taaagggcct gaacgagtat atcaacctct acaaccagaa aaccaagcag
     1861 aaactgccta agttcaagcc actgtacaaa caagtgctct cagataggga aagcctgagc
     1921 ttctacggtg aagggtatac atcagatgaa gaagtgctcg aagtgttccg caacaccctc
     1981 aataagaaca gtgaaatctt ctcttcaatc aagaagctgg agaaactgtt caagaatttc
     2041 gatgagtact cctctgccgg aatctttgtg aagaatggcc ctgcaatatc cactattagc
     2101 aaagacatct ttggcgagtg gaacgttatc agggataagt ggaatgccga gtacgatgat
     2161 attcatctca agaagaaagc cgtggttaca gagaaatacg aggatgatag acgcaagagc
     2221 tttaagaaga ttggtagctt ctctctcgaa cagctgcagg agtacgccga cgctgacctg
     2281 tcagtcgtgg agaaactcaa ggagatcata atccagaagg tggatgaaat ctacaaagtg
     2341 tatggaagct ctgagaaact cttcgatgca gactttgttc tggagaagag tctgaagaag
     2401 aacgacgcag tggttgctat catgaaggac ctgctggatt ctgttaagtc tttcgagaat
     2461 tacattaagg cattctttgg tgaagggaag gagacaaata gggacgagag cttctatggc
     2521 gactttgttc tggcctacga catcctcctc aaggttgacc acatctatga cgctatacgg
     2581 aattacgtta cccagaagcc ctatagcaaa gacaagttca agctgtattt ccagaatcca
     2641 cagtttatgg gtgggtggga taaagacaaa gaaacagatt acagggccac tatcctgcgg
     2701 tacggcagca aatactatct ggctatcatg gataagaagt acgccaaatg cctccagaag
     2761 atcgacaagg acgacgtgaa cggtaactac gagaagatca attacaagct cctgccagga
     2821 cctaacaaga tgctgcccaa ggtgttcttc tccaagaaat ggatggccta ctataaccca
     2881 agcgaggaca ttcagaagat atacaagaat gggacattca agaagggcga tatgttcaac
     2941 ctcaacgact gccacaagct gattgatttc ttcaaggata gcatttctcg ctatcccaag
     3001 tggtctaatg catacgattt caacttcagc gagactgaga agtacaaaga catcgctggc
     3061 ttctaccggg aggtggaaga gcaaggctat aaggtgtcat tcgaatccgc ttctaagaag
     3121 gaagtggata agctcgtgga agagggtaag ctgtacatgt tccagatata caacaaagac
     3181 ttcagcgata agagccacgg cactccaaac ctccatacta tgtatttcaa gctgctgttt
     3241 gacgagaaca accacggaca gattaggctg tcaggaggcg cagaactctt catgcgcaga
     3301 gcttcactga agaaggagga actcgttgtc cacccagcca atagccctat agccaataag
     3361 aatccagaca atcctaagaa aaccactact ctgtcttacg atgtgtataa ggataagaga
     3421 ttctctgaag atcagtacga actgcacata cccattgcca ttaacaagtg ccctaagaac
     3481 atcttcaaga ttaacacaga ggttagagtg ctcctgaaac acgacgataa cccttatgtt
     3541 ataggcattg ctcgcggaga gagaaacctg ctgtacatcg tcgtggtgga cggcaaaggc
     3601 aacatcgtgg aacagtacag tctcaatgaa atcattaaca atttcaacgg aatccgcatt
     3661 aagaccgact accattctct cctcgacaag aaggagaaag aaaggttcga agcaagacag
     3721 aattggacaa gtatagagaa tatcaaagaa ctgaaggctg ggtacatctc tcaggttgtg
     3781 cacaagatat gtgagctggt ggagaagtac gacgctgtta tcgccctcgc ggacctgaat
     3841 agcggcttca agaactccag ggtgaaggtg gagaagcagg tgtatcagaa gttcgagaag
     3901 atgctgatcg acaagctcaa ctatatggtg gacaagaaat ccaatccttg cgctactggt
     3961 ggagccctga agggctatca aatcaccaat aagttcgaat ctttcaagtc tatgagcacc
     4021 cagaatggct tcatcttcta catacccgca tggctgacat ccaagattga tccctctacc
     4081 ggatttgtta atctgctcaa gactaagtac acctctattg ctgactcaaa gaagttcata
     4141 tcatcatttg accgcatcat gtacgtgcca gaagaggacc tgttcgagtt tgccctggat
     4201 tacaagaatt tctctcggac tgacgccgac tacatcaaga agtggaagct ctactcttat
     4261 ggtaatcgga ttcgcatatt ccgcaatccc aagaagaata acgtgttcga ttgggaggaa
     4321 gtttgcctca ccagcgctta caaggagctg ttcaataagt atgggattaa ctaccagcag
     4381 ggcgacataa gagccctgct gtgcgaacaa tctgataagg cattctattc ctctttcatg
     4441 gcactgatgt cactgatgct gcaaatgcgc aattccatca ccggaagaac agacgtggcc
     4501 tttctgatct ctcctgtcaa gaactcagat ggcatcttct acgattcccg caactatgaa
     4561 gcacaggaga atgctatcct gcctaagaat gccgatgcaa atggagccta taacatcgcc
     4621 agaaaggtcc tctgggccat aggacaattc aagaaagctg aagatgagaa gctggacaag
     4681 gtgaagatcg ccatttcaaa caaagagtgg ctcgaatatg ctcagacctc agtgaagcat
     4741 ggatcaccca agaagaaacg gaaagtgtct ggtggttcta ctaatctgtc agatattatt
     4801 gaaaaggaga ccggtaagca actggttatc caggaatcca tcctcatgct cccagaggag
     4861 gtggaagaag tcattgggaa caagccggaa agcgatatac tcgtgcacac cgcctacgac
     4921 gagagcaccg acgagaatgt catgcttctg actagcgacg cccctgaata caagccttgg
     4981 gctctggtca tacaggatag caacggtgag aacaagatta agatgctctc tggtggttct
     5041 cccaagaaga agaggaaagt cgggcccgag ggcagaggaa gtctgctaac atgcggtgac
     5101 gtcgaggaga atcctggccc aactaatctg tcagatatta ttgaaaagga gaccggtaag
     5161 caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg
     5221 aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat
     5281 gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat
     5341 agcaacggtg agaacaagat taagatgctc cccaagaaga agaggaaagt cgctactaac
     5401 ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctac caacctgtcc
     5461 gatatcattg agaaagagac cggcaaacag ctggtgatcc aggagagcat cctgatgctg
     5521 cccgaagagg tggaggaagt gatcggcaac aagcccgagt ccgacatcct ggtgcacaca
     5581 gcctatgatg aatccaccga cgagaacgtg atgctgctga cctccgatgc tcccgagtat
     5641 aaaccctggg cactggtgat ccaggactct aatggagaga acaagatcaa gatgctgccc
     5701 aagaagaaga ggaaagtcga aggacggggc tccctcctga cctgtggcga tgtggaagag
     5761 aaccccggcc ccacaaacct cagtgacatt atcgagaagg aaacaggaaa acagctcgtc
     5821 attcaagaat ctattcttat gttgcctgag gaagtcgaag aggttattgg caataaacct
     5881 gaatctgata ttcttgtcca taccgcttac gatgagtcca cagatgaaaa tgttatgctg
     5941 ctcacatctg acgcaccaga gtacaaacca tgggcgctcg ttattcaaga ttccaacggc
     6001 gaaaacaaaa tcaaaatgct ttctggtggt tctcctaaaa aaaaaagaaa ggtgggctcg
     6061 ggccagtgta ctaattatgc tctcttgaaa ttggctggag atgttgagag caacccaggt
     6121 cccgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     6181 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     6241 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     6301 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     6361 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6421 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6481 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6541 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6601 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6661 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6721 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6781 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     6841 atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6901 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6961 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     7021 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     7081 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     7141 cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7201 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7261 cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7321 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcgggaagag
     7381 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7441 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7501 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7561 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7621 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7681 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7741 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7801 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7861 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7921 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7981 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8041 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8101 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8161 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8221 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8281 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8341 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8401 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8461 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8521 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8581 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8641 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8701 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8761 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8821 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8881 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8941 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9001 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9061 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9121 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9181 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9301 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc
     9361 ccgatcccct agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     9421 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     9481 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     9541 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     9601 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     9661 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     9721 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     9781 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatc
//
