LOCUS       sequence-422062-       16224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full length human LINE-1 in human cells (native L1RP 
            sequence), with a C-terminal 3xFlag tag on ORF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16224)
  AUTHORS   Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, 
            Sanchez R, Miller BD, Di Stefano LH, Ruiz FX, Hancock M, Isik E, 
            Mendez-Dorantes C, Walpole T, Nichols C, Wan P, Riento K, Halls-Kass
            R, Augustin M, Lammens A, Jestel A, Upla P, Xibinaku K, Congreve S, 
            Hennink M, Rogala KB, Schneider AM, Fairman JE, Christensen SM, 
            Desrosiers B, Bisacchi GS, Saunders OL, Hafeez N, Miao W, Kapeller 
            R, Zaller DM, Sali A, Weichenrieder O, Burns KH, Gotte M, Rout MP, 
            Arnold E, Greenbaum BD, Romero DL, LaCava J, Taylor MS
  TITLE     Structures, functions and adaptations of the human LINE-1 ORF2 
            protein.
  JOURNAL   Nature. 2024 Feb;626(7997):194-206. doi: 10.1038/s41586-023-06947-z.
            Epub 2023 Dec 14.
  PUBMED    38096902
REFERENCE   2  (bases 1 to 16224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06947-z"; journalName: "Nature"; date: 
            "2024-02"; volume: "626"; issue: "7997"; pages: "194-206"
COMMENT     Sequence Label: pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promot...
FEATURES             Location/Qualifiers
     source          1..16224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5924..5947
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5948..6013
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    6393..6527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6417..6436)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6471..6490
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6670..6692)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7256..9045
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(9347..11272)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(11707..11725)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11793..11897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11898..11966,11967..12758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12116..12135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12929..13517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13418..13437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        13712..14211
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13747..13770)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     14144..14163
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             14251..14850
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(14251..14270)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     14747..14767
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        15628..16007
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16008..16211
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     16162..16182
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     join(16208..16224,1..8)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgctctagcc
       61 ctggaatgtg tattaagact gtaaggtggg ggggaggagc caagatggcc gaataggaac
      121 agctccggtc tacagctccc agcgtgagcg acgcagaaga cggtgatttc tgcatttcca
      181 tctgaggtac cgggttcatc tcactaggga gtgccagaca gtgggcgcag gccagtgtgt
      241 gtgcgcaccg tgcgcgagcc gaagcagggc gaggcattgc ctcacctggg aagcgcaagg
      301 ggtcagggag ttccctttcc gagtcaaaga aaggggtgac ggacgcacct ggaaaatcgg
      361 gtcactccca cccgaatatt gcgcttttca gaccggctta agaaacggcg caccacgaga
      421 ctatatcccg cacctggctc ggagggtcct acgcccacgg aatctcgctg attgctagca
      481 cagcagtctg agatcaaact gcaaggcggc aacgaggctg ggggaggggc gcccgccatt
      541 gcccaggctt gcttaggtaa acaaagcagc agggaagctc gaactgggtg gagcccacca
      601 cagctcaagg aggcctgcct gcctctgtag gctccacctc tgggggcagg gcacagacaa
      661 acaaaaagac agcagtaacc tctgcagact taagtgtccc tgtctgacag ctttgaagag
      721 agcagtggtt ctcccagcac gcagctggag atctgagaac gggcagactg cctcctcaag
      781 tgggtccctg acccctgacc cccgagcagc ctaactggga ggcacccccc agcaggggca
      841 cactgacacc tcacacggca gggtattcca acagacctgc agctgagggt cctgtctgtt
      901 agaaggaaaa ctaacaacca gaaaggacat ctacaccgaa aacccatctg tacatcacca
      961 tcatcaaaga ccaaaagtag ataaaaccac aaagatgggg aaaaaacaga acagaaaaac
     1021 tggaaactct aaaacgcaga gcgcctctcc tcctccaaag gaacgcagtt cctcaccagc
     1081 aacagaacaa agctggatgg agaatgattt tgatgagctg agagaagaag gcttcagacg
     1141 atcaaattac tctgagctac gggaggacat tcaaaccaaa ggcaaagaag ttgaaaactt
     1201 tgaaaaaaat ttagaagaat gtataactag aataaccaat acagagaagt gcttaaagga
     1261 gctgatggag ctgaaaacca aggctcgaga actacgtgaa gaatgcagaa gcctcaggag
     1321 ccgatgcgat caactggaag aaagggtatc agcaatggaa gatgaaatga atgaaatgaa
     1381 gcgagaaggg aagtttagag aaaaaagaat aaaaagaaat gagcaaagcc tccaagaaat
     1441 atgggactat gtgaaaagac caaatctacg tctgattggt gtacctgaaa gtgatgtgga
     1501 gaatggaacc aagttggaaa acactctgca ggatattatc caggagaact tccccaatct
     1561 agcaaggcag gccaacgttc agattcagga aatacagaga acgccacaaa gatactcctc
     1621 gagaagagca actccaagac acataattgt cagattcacc aaagttgaaa tgaaggaaaa
     1681 aatgttaagg gcagccagag agaaaggtcg ggttaccctc aaaggaaagc ccatcagact
     1741 aacagcggat ctctcggcag aaaccctaca agccagaaga gagtgggggc caatattcaa
     1801 cattcttaaa gaaaagaatt ttcaacccag aatttcatat ccagccaaac taagcttcat
     1861 aagtgaagga gaaataaaat actttataga caagcaaatg ttgagagatt ttgtcaccac
     1921 caggcctgcc ctaaaagagc tcctgaagga agcgctaaac atggaaagga acaaccggta
     1981 ccagccgctg caaaatcatg ccaaaatgta aagaccatca agactaggaa gaaactgcac
     2041 caactaatga gcaaaatcac cagctaacat cataatgaca ggatcaactt cacacataac
     2101 aatattaact ttaaatataa atggactaaa ttctgcaatt aaaagacaca gactggcaag
     2161 ttggataaag agtcaagacc catcagtgtg ctgtattcag gaaacccatc tcacgtgcag
     2221 agacacacat aggctcaaaa taaaaggatg gaggaagatc taccaagcca atggaaaaca
     2281 aaaaaaggca ggggttgcaa tcctagtctc tgataaaaca gactttaaac caacaaagat
     2341 caaaagagac aaagaaggcc attacataat ggtaaaggga tcaattcaac aagaggagct
     2401 aactatccta aatatttatg cacccaatac aggagcaccc agattcataa agcaagtcct
     2461 cagtgaccta caaagagact tagactccca cacattaata atgggagact ttaacacccc
     2521 actgtcaaca ttagacagat caacgagaca gaaagtcaac aaggataccc aggaattgaa
     2581 ctcagctctg caccaagcag acctaataga catctacaga actctccacc ccaaatcaac
     2641 agaatatacg tttttttcag caccacacca cacctattcc aaaattgacc acatagttgg
     2701 aagcaaagct ctcctcagca aatgtaaaag aacagaaatt ataacaaact atctctcaga
     2761 ccacagtgca atcaaactag agctcaggat taagaatctc actcaaagcc gctcaactac
     2821 atggaaactg aacaacctgc tcctgaatga ctactgggta cataacgaaa tgaaggcaga
     2881 aataaagatg ttctttgaaa ccaacgagaa caaagacacc acataccaga atctctggga
     2941 cgcattcaaa gcagtatgta gagggaaatt tatagcacta aatgcctaca agagaaagca
     3001 ggaaagatcc aaaattgaca ccctaacatc acaattaaaa gaactagaaa agcaagagca
     3061 aacacattca aaagctagca gaaggcaaga aataactaaa atcagagcag aactgaagga
     3121 aatagagaca caaaaaaccc ttcaaaaaat caatgaatcc aggagctggt tttttgaaag
     3181 gatcaacaaa attgatagac cgctagcaag actaataaag aaaaaaagag agaagaatca
     3241 aatagacaca ataaaaaatg ataaagggga tatcaccacc gatcccacag aaatacaaac
     3301 taccatcaga gaatactaca aacacctcta cgcaaataaa ctagaaaatc tagaagaaat
     3361 ggatacattc ctcgacacat acactctccc aagactaaac caggaagaag ttgaatctct
     3421 gaatcgacca ataacaggct ctgaaattgt ggcaataatc aatagtttac caaccaaaaa
     3481 gagtccagga ccagatggat tcacagccga attctaccag aggtacaagg aggaactggt
     3541 accattcctt ctgaaactat tccaatcaat agaaaaagag ggaatcctcc ctaactcatt
     3601 ttatgaggcc agcatcattc tgataccaaa gccgggcaga gacacaacca aaaaagagaa
     3661 ttttagacca atatccttga tgaacattga tgcaaaaatc ctcaataaaa tactggcaaa
     3721 ccgaatccag cagcacatca aaaagcttat ccaccatgat caagtgggct tcatccctgg
     3781 gatgcaaggc tggttcaata tacgcaaatc aataaatgta atccagcata taaacagagc
     3841 caaagacaaa aaccacatga ttatctcaat agatgcagaa aaagcctttg acaaaattca
     3901 acaacccttc atgctaaaaa ctctcaataa attaggtatt gatgggacgt atttcaaaat
     3961 aataagagct atctatgaca aacccacagc caatatcata ctgaatgggc aaaaactgga
     4021 agcattccct ttgaaaaccg gcacaagaca gggatgccct ctctcaccgc tcctattcaa
     4081 catagtgttg gaagttctgg ccagggcaat caggcaggag aaggaaataa agggtattca
     4141 attaggaaaa gaggaagtca aattgtccct gtttgcagac gacatgattg tttatctaga
     4201 aaaccccatc gtctcagccc aaaatctcct taagctgata agcaacttca gcaaagtctc
     4261 aggatacaaa atcaatgtac aaaaatcaca agcattctta tacaccaaca acagacaaac
     4321 agagagccaa atcatgggtg aactcccatt cacaattgct tcaaagagaa taaaatacct
     4381 aggaatccaa cttacaaggg atgtgaagga cctcttcaag gagaactaca aaccactgct
     4441 caaggaaata aaagaggaga caaacaaatg gaagaacatt ccatgctcat gggtaggaag
     4501 aatcaatatc gtgaaaatgg ccatactgcc caaggtaatt tacagattca atgccatccc
     4561 catcaagcta ccaatgactt tcttcacaga attggaaaaa actactttaa agttcatatg
     4621 gaaccaaaaa agagcccgca ttgccaagtc aatcctaagc caaaagaaca aagctggagg
     4681 catcacacta cctgacttca aactatacta caaggctaca gtaaccaaaa cagcatggta
     4741 ctggtaccaa aacagagata tagatcaatg gaacagaaca gagccctcag aaataatgcc
     4801 gcatatctac aactatctga tctttgacaa acctgagaaa aacaagcaat ggggaaagga
     4861 ttccctattt aataaatggt gctgggaaaa ctggctagcc atatgtagaa agctgaaact
     4921 ggatcccttc cttacacctt atacaaaaat caattcaaga tggattaaag atttaaacgt
     4981 taaacctaaa accataaaaa ccctagaaga aaacctaggc attaccattc aggacatagg
     5041 cgtgggcaag gacttcatgt ccaaaacacc aaaagcaatg gcaacaaaag acaaaattga
     5101 caaatgggat ctaattaaac taaagagctt ctgcacagca aaagaaacta ccatcagagt
     5161 gaacaggcaa cctacaacat gggagaaaat ttttgcaacc tactcatctg acaaagggct
     5221 aatatccaga atctacaatg aactcaaaca aatttacaag aaaaaaacaa acaaccccat
     5281 caaaaagtgg gcgaaggaca tgaacagaca cttctcaaaa gaagacattt atgcagccaa
     5341 aaaacacatg aagaaatgct catcatcact ggccatcaga gaaatgcaaa tcaaaaccac
     5401 tatgagatat catctcacac cagttagaat ggcaatcatt aaaaagtcag gaaacaacag
     5461 gtgctggaga ggatgcggag aaataggaac acttttacac tgttggtggg actgtaaact
     5521 agttcaacca ttgtggaagt cagtgtggcg attcctcagg gatctagaac tagaaatacc
     5581 atttgaccca gccatcccat tactgggtat atacccaaat gagtataaat catgctgcta
     5641 taaagacaca tgcacacgta tgtttattgc ggcactattc acaatagcaa agacttggaa
     5701 ccaacccaaa tgtccaacaa tgatagactg gattaagaaa atgtggcaca tatacaccat
     5761 ggaatactat gcagccataa aaaatgatga gttcatatcc tttgtaggga catggatgaa
     5821 attggaaacc atcattctca gtaaactatc gcaagaacaa aaaaccaaac accgcatatt
     5881 ctcactcata ggtgggaatg gcggcggcag cggcggcggc agcctggaag ttctgttcca
     5941 ggggcccgac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
     6001 tgacgatgac aagtagacaa tgagatcaca tggacacagg aaggggaata tcacactctg
     6061 gggactgtgg tggggtcggg ggagggggga gggatagcat tgggagatat acctaatgct
     6121 agatgacaca ttagtgggtg cagcgcacca gcatggcaca tgtaaaacaa tgagatcaca
     6181 tggacacagg aaggggaata tcacactctg gggactgtgg tggggtcggg ggagggggga
     6241 gggatagcat tgggagatat acctaatgct agatgacaca ttagtgggtg cagcgcacca
     6301 gcatggcaca tgtatacata tgtaacttaa gggcgcgcca atgtgcacat gtaccctgca
     6361 ggggccggcc taaaacttag agtataataa aggatccaga catgataaga tacattgatg
     6421 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     6481 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     6541 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     6601 acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga
     6661 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     6721 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     6781 agccatgagg tcgatcgact ctagaggatc gatccccgcc ccggacgaac taaacctgac
     6841 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     6901 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     6961 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     7021 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     7081 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     7141 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     7201 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     7261 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     7321 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     7381 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     7441 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     7501 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     7561 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     7621 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     7681 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     7741 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     7801 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     7861 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     7921 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     7981 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     8041 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     8101 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     8161 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     8221 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     8281 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     8341 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     8401 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     8461 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     8521 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     8581 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     8641 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     8701 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     8761 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     8821 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     8881 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat
     8941 atacagtcag catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc
     9001 cacctcccaa gggggcgtga attttcgctg cttgtccttt tcctgctggt tgctcccatt
     9061 cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta
     9121 aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca
     9181 acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc
     9241 cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga
     9301 atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt
     9361 cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg
     9421 cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc
     9481 acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg
     9541 catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa
     9601 tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac
     9661 gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt
     9721 aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg
     9781 tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa
     9841 atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc
     9901 tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg
     9961 ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt
    10021 ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg
    10081 gcctgcgcgg tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt
    10141 ctccacgtcc acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac
    10201 gtcctctacc ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac
    10261 cccggcctcc actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc
    10321 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
    10381 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
    10441 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
    10501 cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
    10561 ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
    10621 cccctcctgc tcctgcccct cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg
    10681 ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc
    10741 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc
    10801 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc
    10861 ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
    10921 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgctcctg
    10981 cccctcccgc tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac
    11041 ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc
    11101 ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt
    11161 tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc
    11221 ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca
    11281 ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga
    11341 ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga
    11401 tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc
    11461 agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc
    11521 tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc
    11581 tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt
    11641 gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac
    11701 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
    11761 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    11821 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    11881 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    11941 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    12001 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    12061 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    12121 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
    12181 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    12241 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    12301 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    12361 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    12421 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    12481 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    12541 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    12601 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    12661 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    12721 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    12781 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    12841 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    12901 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    12961 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    13021 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    13081 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    13141 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    13201 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    13261 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    13321 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    13381 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    13441 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    13501 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    13561 ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg
    13621 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc
    13681 gcgtcgaatt cgtcgacctc gaaattctac cgggtagggg aggcgctttt cccaaggcag
    13741 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
    13801 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
    13861 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
    13921 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
    13981 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
    14041 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
    14101 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
    14161 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcatc
    14221 catctagatc tcgagcagct gaagcttacc atgaccgagt acaagcccac ggtgcgcctc
    14281 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
    14341 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
    14401 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
    14461 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
    14521 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
    14581 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
    14641 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
    14701 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
    14761 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
    14821 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
    14881 gaaaggagcg cacgacccca tgcatcgatg atatcagatc cccgggatgc agaaattgat
    14941 gatctattaa acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta
    15001 agaagggtga gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg
    15061 ggtggggtgg gattagataa atgcctgctc tttactgaag gctctttact attgctttat
    15121 gataatgttt catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc
    15181 tcattcctcc cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct
    15241 cttgattccc actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc
    15301 tgaagaacga gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc
    15361 aagttctaat tccatcagaa gctggtcgac caattctcat gtttgacagc ttatcatcgc
    15421 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
    15481 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
    15541 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
    15601 gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat
    15661 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
    15721 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
    15781 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
    15841 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg
    15901 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact
    15961 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
    16021 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
    16081 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
    16141 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
    16201 taagcagagc tcgtttagtg aacc
//
