LOCUS       sequence-422111-       11515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 Spike_Beta in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, 
            Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar
            S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, 
            Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne 
            Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
  TITLE     Human protein interaction networks of ancestral and variant 
            SARS-CoV-2 in organ-specific cells and bodily fluids.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
  PUBMED    40593736
REFERENCE   2  (bases 1 to 11515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5784. doi"; pages: " 
            10.1038/s41467-025-60949-1"
COMMENT     Sequence Label: pLD-puro-Cc-SARS-CoV-2_Spike_Beta-VA
FEATURES             Location/Qualifiers
     source          1..11515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     761..781
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1070..1273
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1224..1244
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1270..1294
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1325..1349
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5131..5155)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5235..5258
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5295..5318
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5331..5348
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5358..5378
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5391..5411
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5424..5489
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5537..5654
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5703..6213
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6132..6150
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6235..6834
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6235..6254)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6731..6751
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6962..7195
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7267..7388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7304..7323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7358..7377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7428..7563
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7583..7602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7584..7602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7612..7629)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7612..7628)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7621..7643)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7770..8225
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7857..7876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8067..8088
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8251..8355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8356..8424,8425..9216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8574..8593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9387..9975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9876..9895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10129..10146
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10263..10284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10299..10304,10305..10322,10323..10329)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10337..10353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10342..10364
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10361..10377
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10361..10377
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10396..10416
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10398..10416
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10444..10670
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10671..10851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10898..11023
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat
      781 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
      841 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      901 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      961 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1021 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1081 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1141 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1201 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1261 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgtac
     1321 gcgtacaagt ttgtacaaaa aagttggcat gttcgtgttc ctggtgctgc tgccactggt
     1381 gagctcccag tgcgtgaacc tgaccaccag aacccagctg ccacctgcct acaccaactc
     1441 cttcaccagg ggcgtgtact acccagacaa ggtgttcaga tccagcgtgc tgcactctac
     1501 ccaggacctg ttcctgccat tcttctctaa cgtgacctgg ttccacgcca tccacgtgtc
     1561 tggaaccaac ggcaccaaga gattcgccaa cccagtgctg ccattcaacg acggcgtgta
     1621 cttcgcttct accgagaagt ctaacatcat caggggttgg atcttcggaa ccaccctgga
     1681 ctctaagacc cagtctctgc tgatcgtgaa caacgctacc aacgtggtga tcaaggtgtg
     1741 cgagttccag ttctgcaacg acccattcct gggagtgtac taccacaaga acaacaagtc
     1801 ttggatggaa agcgagttca gggtgtacag ctctgctaac aactgcacct tcgagtacgt
     1861 gtctcagcct ttcctgatgg acctggaagg aaagcaggga aacttcaaga acctgagaga
     1921 gttcgtgttc aagaacatcg acggttactt caagatatac agcaagcaca ccccaatcaa
     1981 cctggtgagg ggcctgccac agggtttctc tgctctggag cccctggtgg acctgccaat
     2041 cggcatcaac atcaccaggt tccagaccct gcacaggtct tacctgaccc caggcgacag
     2101 cagctctgga tggaccgccg gcgccgctgc ttactacgtg ggatacctgc agccaaggac
     2161 cttcctgctg aagtacaacg aaaacggcac catcaccgac gccgtggact gcgctctgga
     2221 cccactgtct gagaccaagt gcaccctgaa gtctttcacc gtggaaaagg gaatctacca
     2281 gaccagcaac ttccgcgtgc agcccaccga gtctatcgtg agattcccaa acatcaccaa
     2341 cctgtgccca ttcggagaag tgttcaacgc taccaggttc gccagcgtgt acgcttggaa
     2401 caggaagaga atcagcaact gcgtggccga ctactctgtg ctgtacaact ctgcctcctt
     2461 ctccaccttc aagtgctacg gcgtgtcccc aaccaagctg aacgacctgt gcttcaccaa
     2521 cgtgtacgct gactccttcg tgatcagagg agacgaagtg agacagatcg ctcccggtca
     2581 gaccggaaac atcgccgact acaactacaa gctgccagac gacttcaccg gttgcgtgat
     2641 cgcttggaac tctaacaacc tggactctaa ggtgggaggc aactacaact acctgtacag
     2701 gctgttccgc aagagcaacc tgaagccttt cgaaagggac atcagcaccg aaatctacca
     2761 ggctggatct accccatgca acggagtgaa gggattcaac tgctacttcc ctctgcagtc
     2821 ttacggattc cagccaacct acggagtggg ataccagcca tacagagtgg tggtgctgtc
     2881 tttcgagctg ctgcacgctc ctgctaccgt gtgcggacca aagaagtcta ccaacctggt
     2941 gaagaacaag tgcgtgaact tcaacttcaa cggactgacc ggaaccggag tgctgaccga
     3001 atccaacaag aagttcctgc ctttccagca gttcggtaga gacatcgccg acaccaccga
     3061 cgctgtgaga gacccacaga ccctggaaat cctggacatc accccctgct ctttcggagg
     3121 agtgagcgtg atcaccccag gaaccaacac ctccaaccag gtggccgtgc tgtaccaggg
     3181 cgtgaactgc accgaagtgc cagtggccat ccacgctgac cagctgaccc caacctggag
     3241 agtgtacagc accggatcta acgtgttcca gacccgtgcc ggctgcctga tcggagccga
     3301 gcacgtgaac aactcctacg aatgcgacat cccaatcgga gctggcatct gcgcttctta
     3361 ccagacccag accaacagcc caagaagggc cagatccgtg gcctctcaga gcatcatcgc
     3421 ctacaccatg agcctgggcg tggaaaacag cgtggcttac agcaacaact ctatcgccat
     3481 cccaaccaac ttcaccatct ctgtgaccac cgaaatcctg ccagtgagca tgaccaagac
     3541 cagcgtggac tgcaccatgt acatctgcgg agactctacc gaatgctcta acctgctgct
     3601 gcagtacggc agcttctgca cccagctgaa ccgcgctctg accggaatcg ctgtggagca
     3661 ggacaagaac acccaggaag tgttcgctca ggtgaagcag atatacaaga ccccacctat
     3721 caaggacttc ggaggattca acttctctca gatcctgccc gacccatcta ctgctgttca
     3781 acaaggtgac cctggccgac gccggtttca tcaagcagta cggtgactgc ctgggagaca
     3841 tcgccgctag agacctgatc tgcgcccaga agttcaacgg tctgaccgtg ctgccaccac
     3901 tgctgaccga cgagatgatc gcccagtaca cctctgccct gctggctgga accatcacct
     3961 ctggttggac cttcggagct ggcgccgccc tgcagatccc attcgctatg cagatggcct
     4021 accgtttcaa cggtatcgga gtgacccaga acgtgctgta cgaaaaccag aagctgatcg
     4081 ctaaccagtt caactctgct atcggaaaga tccaggactc cctgtcctct accgcttctg
     4141 ccctgggaaa gctgcaggac gtggtgaacc agaacgccca ggccctgaac accctggtga
     4201 agcagctgtc ttctaacttc ggagccatct cctctgtgct gaacgacatc ctgtctaggc
     4261 tggacaaggt ggaagccgag gtgcagatcg acagactgat caccggaaga ctgcagtctc
     4321 tgcagaccta cgtgacccag cagctgatca gggccgctga aatcagagct tctgccaacc
     4381 tggctgccac caagatgtcc gaatgcgtgc tgggccagtc taagagggtg gacttctgcg
     4441 gaaagggata ccacctgatg tccttcccac agtctgctcc acacggagtg gtgttcctgc
     4501 acgtgaccta cgtgcccgcc caggaaaaga acttcaccac cgccccagct atctgccacg
     4561 acggaaaggc tcacttcccc agggagggag tgttcgtgtc taacggaacc cactggttcg
     4621 tgacccagag gaacttctac gagccacaga tcatcaccac cgacaacacc ttcgtgtctg
     4681 gaaactgcga cgtggtgatc ggaatcgtga acaacaccgt gtacgaccca ctgcagccag
     4741 aactggactc tttcaaggag gaactggaca agtacttcaa gaaccacacc tctccagacg
     4801 tggacctggg agacatctcc ggaatcaacg cctctgtggt gaacatccag aaggaaatcg
     4861 acagactgaa cgaggtggcc aagaacctga acgaatctct gatcgacctg caggaactgg
     4921 gaaagtacga gcagtacatc aagtggccct ggtacatctg gctgggtttc atcgctggtc
     4981 tgatcgctat cgtgatggtg accatcatgc tgtgctgcat gacctcttgc tgctcttgcc
     5041 tgaagggatg ctgctcttgc ggatcttgct gcaagttcga cgaggacgac tccgagcctg
     5101 tgctgaaggg tgtgaagctg cactacacct acccaacttt cttgtacaaa gtggttgatt
     5161 ctagagcgat cgccagagag ctgttcaacc tgctgggcga gaaacagccc cccgtggtga
     5221 tcaaggcgat cgcctggagc caccctcagt tcgagaaggg aggaggaagc ggcggaggca
     5281 gcggaggagg aagctggagc cacccgcagt tcgagaaagg cggaagcggc catcaccatc
     5341 accatcaccc tgcaggagaa aatctgtact tccaggggag aggaggcagc gagaacctgt
     5401 actttcaggg gcctgcagga agagactaca aagaccatga cggtgattat aaagatcatg
     5461 acatcgatta caaggatgac gatgacaagt agttcgaatt ctcgacctcg agacaaatgg
     5521 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     5581 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     5641 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg
     5701 agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     5761 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     5821 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     5881 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     5941 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     6001 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     6061 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     6121 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     6181 ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggagct taccatgacc
     6241 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6301 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6361 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6421 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6481 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6541 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6601 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6661 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6721 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6781 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     6841 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggtaccttta
     6901 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6961 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7021 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7081 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7141 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7201 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7261 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7321 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7381 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     7441 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7501 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7561 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     7621 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     7681 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7741 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     7801 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7861 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7921 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7981 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8041 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8101 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8161 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     8221 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8281 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8341 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8401 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8461 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8521 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8581 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8641 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8701 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8761 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8821 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8881 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8941 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9001 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9061 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9121 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9181 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9241 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9301 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9361 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9421 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9481 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9541 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9601 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9661 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9721 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9781 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9841 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9901 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9961 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10021 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10081 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    10141 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    10201 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    10261 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    10321 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    10381 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
    10441 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
    10501 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
    10561 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
    10621 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
    10681 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    10741 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    10801 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
    10861 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    10921 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    10981 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    11041 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    11101 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    11161 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    11221 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    11281 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    11341 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
    11401 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
    11461 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaat
//
