LOCUS       sequence-422113-       11544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 Spike_Alpha in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, 
            Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar
            S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, 
            Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne 
            Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
  TITLE     Human protein interaction networks of ancestral and variant 
            SARS-CoV-2 in organ-specific cells and bodily fluids.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
  PUBMED    40593736
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5784. doi"; pages: " 
            10.1038/s41467-025-60949-1"
COMMENT     Sequence Label: pLD-puro-Cc-SARS-CoV-2_Spike_Alpha-VA
FEATURES             Location/Qualifiers
     source          1..11544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     761..781
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1070..1273
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1224..1244
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1270..1294
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1325..1349
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5160..5184)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5264..5287
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5324..5347
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5360..5377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5387..5407
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5420..5440
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5453..5518
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5566..5683
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5732..6242
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6161..6179
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6264..6863
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6264..6283)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6760..6780
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6991..7224
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7296..7417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7333..7352)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7387..7406
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7457..7592
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7612..7631)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7613..7631)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7641..7658)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7641..7657)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7650..7672)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7799..8254
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7886..7905)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8096..8117
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8280..8384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8385..8453,8454..9245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8603..8622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9416..10004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9905..9924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10158..10175
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10292..10313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10328..10333,10334..10351,10352..10358)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10366..10382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10371..10393
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10390..10406
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10390..10406
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10425..10445
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10427..10445
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10473..10699
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10700..10880
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10927..11052
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat
      781 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
      841 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      901 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      961 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1021 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1081 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1141 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1201 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1261 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgtac
     1321 gcgtacaagt ttgtacaaaa aagttggcat gttcgtgttc ctggtgctgc tgccactggt
     1381 gagctcccag tgcgtgaacc tgaccaccag aacccagctg ccacctgcct acaccaactc
     1441 cttcaccagg ggcgtgtact acccagacaa ggtgttcaga tccagcgtgc tgcactctac
     1501 ccaggacctg ttcctgccat tcttctctaa cgtgacctgg ttccacgcca tctctggaac
     1561 caacggcacc aagagattcg acaacccagt gctgccattc aacgacggcg tgtacttcgc
     1621 ttctaccgag aagtctaaca tcatcagggg ttggatcttc ggaaccaccc tggactctaa
     1681 gacccagtct ctgctgatcg tgaacaacgc taccaacgtg gtgatcaagg tgtgcgagtt
     1741 ccagttctgc aacgacccat tcctgggagt gtaccacaag aacaacaagt cttggatgga
     1801 aagcgagttc agggtgtaca gctctgctaa caactgcacc ttcgagtacg tgtctcagcc
     1861 tttcctgatg gacctggaag gaaagcaggg aaacttcaag aacctgagag agttcgtgtt
     1921 caagaacatc gacggttact tcaagatata cagcaagcac accccaatca acctggtgag
     1981 ggacctgcca cagggtttct ctgctctgga gcccctggtg gacctgccaa tcggcatcaa
     2041 catcaccagg ttccagaccc tgctggctct gcacaggtct tacctgaccc caggcgacag
     2101 cagctctgga tggaccgccg gcgccgctgc ttactacgtg ggatacctgc agccaaggac
     2161 cttcctgctg aagtacaacg aaaacggcac catcaccgac gccgtggact gcgctctgga
     2221 cccactgtct gagaccaagt gcaccctgaa gtctttcacc gtggaaaagg gaatctacca
     2281 gaccagcaac ttccgcgtgc agcccaccga gtctatcgtg agattcccaa acatcaccaa
     2341 cctgtgccca ttcggagaag tgttcaacgc taccaggttc gccagcgtgt acgcttggaa
     2401 caggaagaga atcagcaact gcgtggccga ctactctgtg ctgtacaact ctgcctcctt
     2461 ctccaccttc aagtgctacg gcgtgtcccc aaccaagctg aacgacctgt gcttcaccaa
     2521 cgtgtacgct gactccttcg tgatcagagg agacgaagtg agacagatcg ctcccggtca
     2581 gaccggaaag atcgccgact acaactacaa gctgccagac gacttcaccg gttgcgtgat
     2641 cgcttggaac tctaacaacc tggactctaa ggtgggaggc aactacaact acctgtacag
     2701 gctgttccgc aagagcaacc tgaagccttt cgaaagggac atcagcaccg aaatctacca
     2761 ggctggatct accccatgca acggagtgga aggattcaac tgctacttcc ctctgcagtc
     2821 ttacggattc cagccaacct acggagtggg ataccagcca tacagagtgg tggtgctgtc
     2881 tttcgagctg ctgcacgctc ctgctaccgt gtgcggacca aagaagtcta ccaacctggt
     2941 gaagaacaag tgcgtgaact tcaacttcaa cggactgacc ggaaccggag tgctgaccga
     3001 atccaacaag aagttcctgc ctttccagca gttcggtaga gacatcgacg acaccaccga
     3061 cgctgtgaga gacccacaga ccctggaaat cctggacatc accccctgct ctttcggagg
     3121 agtgagcgtg atcaccccag gaaccaacac ctccaaccag gtggccgtgc tgtaccaggg
     3181 cgtgaactgc accgaagtgc cagtggccat ccacgctgac cagctgaccc caacctggag
     3241 agtgtacagc accggatcta acgtgttcca gacccgtgcc ggctgcctga tcggagccga
     3301 gcacgtgaac aactcctacg aatgcgacat cccaatcgga gctggcatct gcgcttctta
     3361 ccagacccag accaacagcc acagaagggc cagatccgtg gcctctcaga gcatcatcgc
     3421 ctacaccatg agcctgggcg ctgaaaacag cgtggcttac agcaacaact ctatcgccat
     3481 cccaatcaac ttcaccatct ctgtgaccac cgaaatcctg ccagtgagca tgaccaagac
     3541 cagcgtggac tgcaccatgt acatctgcgg agactctacc gaatgctcta acctgctgct
     3601 gcagtacggc agcttctgca cccagctgaa ccgcgctctg accggaatcg ctgtggagca
     3661 ggacaagaac acccaggaag tgttcgctca ggtgaagcag atatacaaga ccccacctat
     3721 caaggacttc ggaggattca acttctctca gatcctgccc gacccatcta agccctctaa
     3781 gaggtctttc atcgaagacc tgctgttcaa caaggtgacc ctggccgacg ccggtttcat
     3841 caagcagtac ggtgactgcc tgggagacat cgccgctaga gacctgatct gcgcccagaa
     3901 gttcaacggt ctgaccgtgc tgccaccact gctgaccgac gagatgatcg cccagtacac
     3961 ctctgccctg ctggctggaa ccatcacctc tggttggacc ttcggagctg gcgccgccct
     4021 gcagatccca ttcgctatgc agatggccta ccgtttcaac ggtatcggag tgacccagaa
     4081 cgtgctgtac gaaaaccaga agctgatcgc taaccagttc aactctgcta tcggaaagat
     4141 ccaggactcc ctgtcctcta ccgcttctgc cctgggaaag ctgcaggacg tggtgaacca
     4201 gaacgcccag gccctgaaca ccctggtgaa gcagctgtct tctaacttcg gagccatctc
     4261 ctctgtgctg aacgacatcc tggccaggct ggacaaggtg gaagccgagg tgcagatcga
     4321 cagactgatc accggaagac tgcagtctct gcagacctac gtgacccagc agctgatcag
     4381 ggccgctgaa atcagagctt ctgccaacct ggctgccacc aagatgtccg aatgcgtgct
     4441 gggccagtct aagagggtgg acttctgcgg aaagggatac cacctgatgt ccttcccaca
     4501 gtctgctcca cacggagtgg tgttcctgca cgtgacctac gtgcccgccc aggaaaagaa
     4561 cttcaccacc gccccagcta tctgccacga cggaaaggct cacttcccca gggagggagt
     4621 gttcgtgtct aacggaaccc actggttcgt gacccagagg aacttctacg agccacagat
     4681 catcaccacc cacaacacct tcgtgtctgg aaactgcgac gtggtgatcg gaatcgtgaa
     4741 caacaccgtg tacgacccac tgcagccaga actggactct ttcaaggagg aactggacaa
     4801 gtacttcaag aaccacacct ctccagacgt ggacctggga gacatctccg gaatcaacgc
     4861 ctctgtggtg aacatccaga aggaaatcga cagactgaac gaggtggcca agaacctgaa
     4921 cgaatctctg atcgacctgc aggaactggg aaagtacgag cagtacatca agtggccctg
     4981 gtacatctgg ctgggtttca tcgctggtct gatcgctatc gtgatggtga ccatcatgct
     5041 gtgctgcatg acctcttgct gctcttgcct gaagggatgc tgctcttgcg gatcttgctg
     5101 caagttcgac gaggacgact ccgagcctgt gctgaagggt gtgaagctgc actacaccta
     5161 cccaactttc ttgtacaaag tggttgattc tagagcgatc gccagagagc tgttcaacct
     5221 gctgggcgag aaccagcccc ccgtggtgat caaggcgatc gcctggagcc accctcagtt
     5281 cgagaaggga ggaggaagcg gcggaggcag cggaggagga agctggagcc acccgcagtt
     5341 cgagaaaggc ggaagcggcc atcaccatca ccatcaccct gcaggagaaa atctgtactt
     5401 ccaggggaga ggaggcagcg agaacctgta ctttcagggg cctgcaggaa gagactacaa
     5461 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagta
     5521 gttcgaattc tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     5581 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     5641 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     5701 cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt ttccaaggca
     5761 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     5821 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     5881 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     5941 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     6001 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     6061 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     6121 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     6181 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     6241 agggggatcc accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6301 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6361 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6421 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6481 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6541 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6601 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6661 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6721 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6781 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6841 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     6901 gcgcacgacc ccatgcatcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6961 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7021 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7081 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7141 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7201 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7261 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     7321 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7381 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     7441 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7501 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7561 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     7621 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7681 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7741 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     7801 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7861 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7921 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7981 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8041 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8101 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     8161 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8221 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     8281 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8341 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8401 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8461 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8521 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8581 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8641 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8701 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8761 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8821 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8881 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8941 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9001 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9061 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9121 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9181 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9241 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9301 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9541 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    10081 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    10141 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    10201 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    10261 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    10321 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    10381 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    10441 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
    10501 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
    10561 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
    10621 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
    10681 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
    10741 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10801 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10861 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10921 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10981 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    11041 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    11101 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    11161 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    11221 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    11281 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    11341 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
    11401 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
    11461 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
    11521 gagagaaaaa agagcagtgg gaat
//
