LOCUS       sequence-422114-       10013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin conformation sensor (VcnCS) with a single directionally 
            asymmetric, force strengthening (DAFS) variant point mutation 
            (E1021A)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Chirasani VR, Khan MAI, Malavade JN, Dokholyan NV, Hoffman BD, 
            Campbell SL
  TITLE     Molecular basis and cellular functions of vinculin-actin directional
            catch bonding.
  JOURNAL   Nat Commun. 2023 Dec 14;14(1):8300. doi: 10.1038/s41467-023-43779-x.
  PUBMED    38097542
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-12-14"; volume: "14(1):8300. doi"; pages: " 
            10.1038/s41467-023-43779-x"
COMMENT     Sequence Label: pcDNA3.1-VcnCS-E1021A
FEATURES             Location/Qualifiers
     source          1..10013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(61..80)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        252..631
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        632..835
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     786..806
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     880..899
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        880..898
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3592..4299
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4864..4866,4867..4869,4870..5583)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4909..4930)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5170..5189)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5517..5538
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5621..5638)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5627..5851
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5897..6325
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5984..6003)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6194..6215
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6334..6354)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6339..6668
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6519..6654
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6581..6600
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6735..7529
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6789..6808)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7399..7418
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7703..7824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7740..7759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7794..7813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7873..7889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7873..7889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7886..7908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7897..7913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7921..7927,7928..7945,7946..7951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7966..7987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8104..8121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8275..8863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8355..8374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9034..9825,9826..9894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9657..9676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9895..9999)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
       61 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
      121 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      181 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      241 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      301 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      361 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      421 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      481 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      541 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      601 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      661 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      721 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      781 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
      841 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      901 gacccaagct ggctagcgtt taaacttaag cttgccatgc ccgtcttcca cacgcgcacc
      961 atcgagagca tcttggagcc cgtggctcag cagatctccc acctggtcat catgcacgag
     1021 gagggggagg tagacggcaa ggccatcccg gacctcaccg cccccgtgtc ggccgtgcag
     1081 gccgctgtca gcaacctggt gcgggttgga aaagaaactg tgcagacaac agaagaccag
     1141 atcttgaaaa gggatatgcc accagcattc atcaaagtag agaatgcctg caccaagctc
     1201 gttcgagcag cccagatgct gcaagcagat ccttattcag taccagctcg tgactaccta
     1261 attgatggat caagaggcat cctttctgga acatcagact tacttctgac atttgatgaa
     1321 gcggaggtcc gtaaaatcat ccgtgtctgc aaaggaatat tggaatatct gactgtggca
     1381 gaagtagtag agactatgga ggatttggtg acatatacaa agaatctagg gccaggaatg
     1441 acaaagatgg cgaaaatgat cgatgagaga caacaggaat taactcatca ggaacatagg
     1501 gttatgctgg tgaactccat gaatactgtg aaggagctat tgcctgtact tatttcagct
     1561 atgaagatct ttgtaaccac aaaaaacact aaaagccagg gaatagaaga ggccttgaaa
     1621 aatcgcaatt tcacagtaga gaaaatgagt gctgagataa atgaaataat ccgtgtatta
     1681 caactcactt cctgggatga agatgcctgg gccagcaagg acactgaagc catgaaaaga
     1741 gctttagccc taatagattc aaagatgaac caggcaaaag gctggctgag agatccaaac
     1801 gcacctccag gggatgctgg tgagcaagca ataaggcaga tccttgatga agctggaaaa
     1861 gcaggagaat tgtgtgcagg caaagaacgc agggagattc tgggaacatg caaaactttg
     1921 ggccaaatga ctgatcaact tgctgatctc cgagctagag gacagggtgc tacaccgatg
     1981 gcaatgcaga aggcacagca ggtgtcacaa ggcctggatt tgctcactgc aaaagtggag
     2041 aatgcagccc ggaaattgga ggccatgaca aactctaagc aggctattgc aaagaagatt
     2101 gatgctgctc agaactggct tgcggatccc aacggcggaa gtgaaggaga agaacacatt
     2161 cgaggaatta tgtctgaagc aaggaaagtt gcagaattgt gtgaggagcc taaagaaaga
     2221 gatgatatcc ttcgctcctt gggggaaatc tctgctctga cagctaagct gtcagatctg
     2281 cgacgacatg ggaaaggcga ctctcctgag gcccgtgcat tggccaagca aatagctaca
     2341 tcacttcaga acttacagtc caaaacaaac agagctgtag caaatactag gccagttaaa
     2401 gctgctgtcc atttggaggg caagattgag caagcccaga ggtggataga taatcctaca
     2461 gttgatgatc ggggagtagg ccaggcagca attcggggtt tggttgcaga aggtcgtcgt
     2521 ctagccaatg tcatgatggg accttatcgt caggacctgc ttgccaaatg tgaccgtgta
     2581 gaccagctgg ctgctcagct tgctgacctt gcagcaagag gggaaggaga gtctcctcag
     2641 gctagggcaa ttgctgctca gcttcaggac tccctgaagg atctcaaagc acggatgcaa
     2701 gaagcaatga cccaggaggt gtctgatgtt ttcagtgaca ctacaacccc tattaagttg
     2761 ttagcagtag cagccactgc tccttctgat actcccaata gagaagaggt gtttgaagaa
     2821 agagcagcaa attttgaaaa ccatgctgct agactgggag caacagcaga aaaagcagct
     2881 gcagttggaa ctgctaataa aactactgtg gaaggcattc aggcaacagt caaatctgca
     2941 agggagctta caccacaggt agtatcggct gctcgaatcc tcctgagaaa tcctggaaat
     3001 caagctgctt atgagcattt tgagacaatg aaaaaccaat ggattgataa tgtagaaaag
     3061 atgacagggc tggtggatga ggccatcgat accaagtctc tgttggatgc atcagaagag
     3121 gctattaaga aggatcttga taaatgtaaa gttgcaatgg ccaatatgca acctcagatg
     3181 ctggtagctg gagccaccag cattgctaga cgagcgaacc gcatcctgct tgtggcaaaa
     3241 cgggaggttg aaaattcaga agaccctaaa ttcagggagg ctgttaaagc agcttctgat
     3301 gagctgagca aaaccatatc accgatggta atggatgcta aagctgtagc aggaaatatc
     3361 tctgatcctg gtttgcagaa gagtttcttg gattctggat acaggattct gggagctgtg
     3421 gccaaagtca gagaagcctt tcagcctcag gagccagact ttccccctcc tcctcctgac
     3481 ctagagcatc tccatctgac tgatgagctt gctcctccaa aaccaccact tccagaaggt
     3541 gaggttcccc cacccagacc accaccccct gaagaaaaag atgaggtcga gatggtgagc
     3601 aagggcgagg agaccacaat gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg
     3661 gagggcaacg tgaatggcca cgccttcgtg atcgagggcg agggcgaggg caagccctac
     3721 gacggcacca acaccatcaa cctggaggtg aaggagggag cccccctgcc cttctcctac
     3781 gacattctga ccaccgcgtt cgcctacggc aacagggcct tcaccaagta ccccgacgac
     3841 atccccaact acttcaagca gtccttcccc gagggctact cttgggagcg caccatgacc
     3901 ttcgaggaca agggcatcgt gaaggtgaag tccgacatct ccatggagga ggactccttc
     3961 atctacgaga tacacctcaa gggcgagaac ttccccccca acggccccgt gatgcagaag
     4021 aagaccaccg gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg cgtgctgaag
     4081 ggcgacgtca agcacaagct gctgctggag ggcggcggcc accaccgcgt tgacttcaag
     4141 accatctaca gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc
     4201 atcgagatcc tgaaccacga caaggactac aacaaggtga ccgtttacga gagcgccgtg
     4261 gcccgcaact ccaccgacgg catggacgag ctgtacaagg cggccgcgga gttcccagag
     4321 cagaaagcag gagaagctat taatcagccc atgatgatgg ctgctaggca gttgcatgac
     4381 gaggctcgga aatggtctag caagggtaac gacatcattg ctgctgctaa acgaatggcg
     4441 ctgctcatgg ctgagatgtc acgcctggtg cgaggaggca gcggaaacaa gcgtgccctc
     4501 atccagtgtg caaaagatat tgctaaggca tcggatgaag tcactcgatt ggccaaagag
     4561 gtggccaagc aatgtactga taaacgcatt agaacaaacc tcttacaggt ctgtgagcga
     4621 atcccaacca tcagcacgca gctcaaaatt ctttccacag tgaaagctac catgctgggc
     4681 aggactaaca tcagcgatga agaatcagaa caggcaactg ccatgttggt tcataacgcc
     4741 cagaacctca tgcagtctgt gaaggaaact gtgagagaag ctgaagcagc atccattaag
     4801 ataagaacag atgccggatt cactctgcgc tgggtcagaa agaccccatg gtatcaggaa
     4861 ttcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     4921 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     4981 tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc
     5041 accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     5101 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5161 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5221 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5281 cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag
     5341 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
     5401 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5461 cactacctga gctaccagtc caaactgagc aaagacccca acgagaagcg cgatcacatg
     5521 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     5581 taaatcgatt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     5641 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5701 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5761 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5821 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     5881 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5941 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6001 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6061 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6121 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6181 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6241 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6301 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     6361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6421 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6481 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6541 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6601 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6661 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     6721 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6781 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     6841 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6901 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6961 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     7021 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     7081 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     7141 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     7201 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     7261 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     7321 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     7381 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     7441 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     7501 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     7561 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     7621 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7681 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7741 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7801 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     7861 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7921 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7981 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8041 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8101 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8161 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8221 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8281 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8341 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8401 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8461 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8521 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8581 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8641 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8701 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8761 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8821 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8881 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8941 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9001 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9061 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaa
//
