LOCUS       sequence-422135-        9822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of base editor enCas12a-A3A-Y130F in mammalian cells for 
            base editing, along with non-fused EGFP fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, 
            Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao
            X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
  TITLE     Multiplexed base editing through Cas12a variant-mediated cytosine 
            and adenine base editors.
  JOURNAL   Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8.
  PUBMED    36323848
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-2"; volume: "5(1):1163. doi"; pages: " 
            10.1038/s42003-022-04152-8"
COMMENT     Sequence Label: pCMV-enCas12a-A3A-Y130F
FEATURES             Location/Qualifiers
     source          1..9822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        145..348
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     299..319
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     390..409
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        390..408
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      416..425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             422..1018
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIFDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             4757..4777
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4790..5038
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5051..5071
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5078..5131
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5132..5380
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5381..5401
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5402..5458
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5459..5707
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5708..5728
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5729..5782
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5783..6031
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6044..6064
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6074..6133
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             join(6134..6136,6137..6850)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6134..6136,6137..6847)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6176..6197)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6437..6456)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6784..6805
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6890..6907)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6896..7120
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7191..7207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7191..7207)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7204..7226)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7215..7231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7239..7245,7246..7263,7264..7269))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7284..7305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7422..7439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7593..8181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7673..7692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8352..9143,9144..9212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8975..8994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9213..9317)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9396..9415)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9587..9822,1..144)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
       61 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      121 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      181 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      241 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      301 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
      361 cgtcagatcc gctagagatc cgcggccgct aatacgactc actataggga gagccgccac
      421 catggaagcc agcccagcat ccgggcccag acacttgatg gatccacaca tattcacttc
      481 caactttaac aatggcattg gaaggcataa gacctacctg tgctacgaag tggagcgcct
      541 ggacaatggc acctcggtca agatggacca gcacaggggc tttctacaca accaggctaa
      601 gaatcttctc tgtggctttt acggccgcca tgcggagctg cgcttcttgg acctggttcc
      661 ttctttgcag ttggacccgg cccagatcta cagggtcact tggttcatct cctggagccc
      721 ctgcttctcc tggggctgtg ccggggaagt gcgtgcgttc cttcaggaga acacacacgt
      781 gagactgcgt atcttcgctg cccgcatctt tgattacgac cccctatata aggaggcact
      841 gcaaatgctg cgggatgctg gggcccaagt ctccatcatg acctacgatg aatttaagca
      901 ctgctgggac acctttgtgg accaccaggg atgtcccttc cagccctggg atggactaga
      961 tgagcacagc caagccctga gtgggaggct gcgggccatt ctccagaatc agggaaacag
     1021 cggcagcgag actcccggga cctcagagtc cgccacaccc gaaagtatgt caaagctgga
     1081 gaaattcacc aactgttata gcctgtctaa gaccctgcgc ttcaaggcaa tcccagtggg
     1141 caagacacaa gagaacattg acaacaaacg gctcctggtg gaggatgaga agagggctga
     1201 agattacaag ggcgttaaga agctgctgga taggtactat ctgtcattca tcaacgatgt
     1261 cctccacagt atcaagctga agaatctgaa caattacatt tctctgttcc ggaagaaaac
     1321 cagaaccgag aaggagaata aggagctgga gaacctggag atcaatctgc ggaaggagat
     1381 cgccaaggcc ttcaagggca acgagggcta caagtccctg tttaagaagg atatcatcga
     1441 gacaatcctg ccagagttcc tggacgataa ggacgagatc gccctggtga acagcttcaa
     1501 tggctttacc acagccttca ccggcttctt taggaacaga gagaatatgt tttccgagga
     1561 ggccaagagc acatccatcg ccttcaggtg tatcaacgag aatctgaccc gctacatctc
     1621 taatatggac atcttcgaga aggtggacgc catctttgat aagcacgagg tgcaggagat
     1681 caaggagaag atcctgaaca gcgactatga tgtggaggat ttctttgagg gcgagttctt
     1741 taactttgtg ctgacacagg agggcatcga cgtgtataac gccatcatcg gcggcttcgt
     1801 gaccgagagc ggcgagaaga tcaagggcct gaacgagtac atcaacctgt ataatcagaa
     1861 aaccaagcag aagctgccta agtttaagcc actgtataag caggtgctga gcgatcggga
     1921 gtctctgagc ttctacggcg agggctatac atccgatgag gaggtgctgg aggtgtttag
     1981 aaacaccctg aacaagaaca gcgagatctt cagctccatc aagaagctgg agaagctgtt
     2041 caagaatttt gacgagtact ctagcgccgg catctttgtg aagaacggcc ccgccatcag
     2101 cacaatctcc aaggatatct tcggcgagtg gaacgtgatc cgggacaagt ggaatgccga
     2161 gtatgacgat atccacctga agaagaaggc cgtggtgacc gagaagtacg aggacgatcg
     2221 gagaaagtcc ttcaagaaga tcggctcctt ttctctggag cagctgcagg agtacgccga
     2281 cgccgatctg tctgtggtgg agaagctgaa ggagatcatc atccagaagg tggatgagat
     2341 ctacaaggtg tatggctcct ctgagaagct gttcgacgcc gattttgtgc tggagaagag
     2401 cctgaagaag aacgacgccg tggtggccat catgaaggac ctgctggatt ctgtgaagag
     2461 cttcgagaat tacatcaagg ccttctttgg cgagggcaag gagacaaaca gggacgagtc
     2521 cttctatggc gattttgtgc tggcctacga catcctgctg aaggtggacc acatctacga
     2581 tgccatccgc aattatgtga cccagaagcc ctactctaag gataagttca agctgtattt
     2641 tcagaaccct cagttcatga ggggctggga caaggatagg gagacagact atcgggccac
     2701 catcctgaga tacggctcca agtactatct ggccatcatg gataagaagt acgccaagtg
     2761 cctgcagaag atcgacaagg acgatgtgaa cggcaattac gagaagatca actataagct
     2821 gctgcccggc cctaataaga tgctgccaaa ggtgttcttt tctaagaagt ggatggccta
     2881 ctataacccc agcgaggaca tccagaagat ctacaagaat ggcacattca agaagggcga
     2941 tatgtttaac ctgaatgact gtcacaagct gatcgacttc tttaaggata gcatctcccg
     3001 gtatccaaag tggtccaatg cctacgattt caacttcagc gagactgaga agtacaaaga
     3061 catcgctggc ttctaccggg aggtggaaga gcaaggctat aaggtgtcat tcgaatccgc
     3121 ttctaagaag gaagtggata agctcgtgga agagggtaag ctgtacatgt tccagatata
     3181 caacaaagac ttcagcgata agagccacgg cactccaaac ctccatacta tgtatttcaa
     3241 gctgctgttt gacgagaaca accacggaca gattaggctg tcaggaggcg cagaactctt
     3301 catgcgcaga gcttcactga agaaggagga actcgttgtc cacccagcca atagccctat
     3361 agccaataag aatccagaca atcctaagaa aaccactact ctgtcttacg atgtgtataa
     3421 ggataagaga ttctctgaag atcagtacga actgcacata cccattgcca ttaacaagtg
     3481 ccctaagaac atcttcaaga ttaacacaga ggttagagtg ctcctgaaac acgacgataa
     3541 cccttatgtt ataggcattg ctcgcggaga gagaaacctg ctgtacatcg tcgtggtgga
     3601 cggcaaaggc aacatcgtgg aacagtacag tctcaatgaa atcattaaca atttcaacgg
     3661 aatccgcatt aagaccgact accattctct cctcgacaag aaggagaaag aaaggttcga
     3721 agcaagacag aattggacaa gtatagagaa tatcaaagaa ctgaaggctg ggtacatctc
     3781 tcaggttgtg cacaagatat gtgagctggt ggagaagtac gacgctgtta tcgccctcgc
     3841 ggacctgaat agcggcttca agaactccag ggtgaaggtg gagaagcagg tgtatcagaa
     3901 gttcgagaag atgctgatcg acaagctcaa ctatatggtg gacaagaaat ccaatccttg
     3961 cgctactggt ggagccctga agggctatca aatcaccaat aagttcgaat ctttcaagtc
     4021 tatgagcacc cagaatggct tcatcttcta catacccgca tggctgacat ccaagattga
     4081 tccctctacc ggatttgtta atctgctcaa gactaagtac acctctattg ctgactcaaa
     4141 gaagttcata tcatcatttg accgcatcat gtacgtgcca gaagaggacc tgttcgagtt
     4201 tgccctggat tacaagaatt tctctcggac tgacgccgac tacatcaaga agtggaagct
     4261 ctactcttat ggtaatcgga ttcgcatatt ccgcaatccc aagaagaata acgtgttcga
     4321 ttgggaggaa gtttgcctca ccagcgctta caaggagctg ttcaataagt atgggattaa
     4381 ctaccagcag ggcgacataa gagccctgct gtgcgaacaa tctgataagg cattctattc
     4441 ctctttcatg gcactgatgt cactgatgct gcaaatgcgc aattccatca ccggaagaac
     4501 agacgtggcc tttctgatct ctcctgtcaa gaactcagat ggcatcttct acgattcccg
     4561 caactatgaa gcacaggaga atgctatcct gcctaagaat gccgatgcaa atggagccta
     4621 taacatcgcc agaaaggtcc tctgggccat aggacaattc aagaaagctg aagatgagaa
     4681 gctggacaag gtgaagatcg ccatttcaaa caaagagtgg ctcgaatatg ctcagacctc
     4741 agtgaagcat ggatcaccca agaagaaacg gaaagtgtct ggtggttcta ctaatctgtc
     4801 agatattatt gaaaaggaga ccggtaagca actggttatc caggaatcca tcctcatgct
     4861 cccagaggag gtggaagaag tcattgggaa caagccggaa agcgatatac tcgtgcacac
     4921 cgcctacgac gagagcaccg acgagaatgt catgcttctg actagcgacg cccctgaata
     4981 caagccttgg gctctggtca tacaggatag caacggtgag aacaagatta agatgctctc
     5041 tggtggttct cccaagaaga agaggaaagt cgggcccgag ggcagaggaa gtctgctaac
     5101 atgcggtgac gtcgaggaga atcctggccc aactaatctg tcagatatta ttgaaaagga
     5161 gaccggtaag caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga
     5221 agtcattggg aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac
     5281 cgacgagaat gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt
     5341 catacaggat agcaacggtg agaacaagat taagatgctc cccaagaaga agaggaaagt
     5401 cgctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctac
     5461 caacctgtcc gatatcattg agaaagagac cggcaaacag ctggtgatcc aggagagcat
     5521 cctgatgctg cccgaagagg tggaggaagt gatcggcaac aagcccgagt ccgacatcct
     5581 ggtgcacaca gcctatgatg aatccaccga cgagaacgtg atgctgctga cctccgatgc
     5641 tcccgagtat aaaccctggg cactggtgat ccaggactct aatggagaga acaagatcaa
     5701 gatgctgccc aagaagaaga ggaaagtcga aggacggggc tccctcctga cctgtggcga
     5761 tgtggaagag aaccccggcc ccacaaacct cagtgacatt atcgagaagg aaacaggaaa
     5821 acagctcgtc attcaagaat ctattcttat gttgcctgag gaagtcgaag aggttattgg
     5881 caataaacct gaatctgata ttcttgtcca taccgcttac gatgagtcca cagatgaaaa
     5941 tgttatgctg ctcacatctg acgcaccaga gtacaaacca tgggcgctcg ttattcaaga
     6001 ttccaacggc gaaaacaaaa tcaaaatgct ttctggtggt tctcctaaaa aaaaaagaaa
     6061 ggtgggctcg ggccagtgta ctaattatgc tctcttgaaa ttggctggag atgttgagag
     6121 caacccaggt cccgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     6181 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     6241 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     6301 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     6361 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     6421 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     6481 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     6541 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     6601 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     6661 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     6721 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     6781 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     6841 gtacaagtaa atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     6901 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6961 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7021 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7081 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     7141 agctggggct cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7201 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7261 aagtgtaaag cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7321 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7381 gcgggaagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7441 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7501 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7561 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7621 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7681 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7741 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7801 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7861 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7921 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7981 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8041 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8101 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8161 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8221 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8281 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8341 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9181 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9241 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9301 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     9361 gatcgatctc ccgatcccct agggtcgact ctcagtacaa tctgctctga tgccgcatag
     9421 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     9481 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     9541 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     9601 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     9661 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     9721 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     9781 caatgggtgg agtatttacg gtaaactgcc cacttggcag ta
//
