LOCUS       sequence-422136-        7718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses miniSOG in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Cuentas-Condori A, Mulcahy B, He S, Palumbos S, Zhen M, Miller DM 
            3rd
  TITLE     C. elegans neurons have functional dendritic spines.
  JOURNAL   Elife. 2019 Oct 4;8. pii: 47918. doi: 10.7554/eLife.47918.
  PUBMED    31584430
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-10-4"; volume: "8. pii: 47918. doi"; pages: " 
            10.7554/eLife.47918"
COMMENT     Sequence Label: pSH83
FEATURES             Location/Qualifiers
     source          1..7718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2665..2985
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(5158..5180)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5280..5299
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5377..5395)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5463..5567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5568..5636,5637..6428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5786..5805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6599..7187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7088..7107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7341..7358
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7475..7496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7511..7516,7517..7534,7535..7541)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7549..7565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7554..7576
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7573..7589
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7573..7589
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatga aattgtgcct cctgatgctg aatcctcaat ccatttctcc
       61 atacttcctt ttcacttcct ccatttggcg gaaggtgggc ggtcctcccg ccgaaacttg
      121 atctcttttt tatttttaag aagatccttt atgtttttta tattgacgtt tacttttttt
      181 ttggaagaaa gtgggtgaac tacaatcaga ttatttttat gctccgcgaa aatttaatca
      241 tgatcaaatg tgatatatga tgataatttg ggaataaaaa agtggaggtg gaacataatt
      301 tgtgttctta cggactataa tttatttgat ataaaacatc attatttcac ttgttgcagt
      361 caaaatgttc agacttgcaa ccgctttttt ctttaacccc atttaaatag gtcattttga
      421 aactgaaatt ttagatatca atgtaacaat tttcagaaaa tcatgaaaat gagaaaataa
      481 cactgaatct caattttttc taactagccg tttattaatt aaaactgtag tagcatttta
      541 gtcggcattt tgtgtcaaaa caaattcatt cttgtatttt tttttaatgc agtcaatatc
      601 ttagtaaatt gccaaatata cgaaacctat ggagcgtttc tgaggaattc agaacactag
      661 aacactgttc aatccttgct acatttaaat acagggtggg ccaaaagtat ggtaacacgt
      721 ttgcagctct ataactttac tagtggagag agttattaca tcaatttgta caaggtttgt
      781 tctccataaa atactgacca gatgaacgtt ttgaattttc aaaatattcc catccgcatc
      841 gctgcaggcc ttcagaactt ttgagatcac gacctttatg gcacgcaagt cggcattctg
      901 cgggatttct acatttttct caaggaatcc atcaagtgtc ttacaagttt ttatagttca
      961 aagaatgctc ttggtctaga gattttttaa aaacaaaaat ccaaataatt tctatcacgg
     1021 tatttctgtc atggactgcg gcagtaggtt aagtcttgaa tggcatagtc gccagaaaga
     1081 tggatgtgag cggcgagtag cagtatgcag aaaaattcat tttagtattt ttcgagacgt
     1141 tttgacctga ctttccagaa tttttttaga aaattgagct cgaatgattt tgtgggtcgt
     1201 tacgccttgc gaaaccaaaa aaactgagta aatttcgcaa agttgaattc atgaaaaatt
     1261 caaaggttct ggcgccagtg tcgacacaga aaatttttgt atgctacaaa aacaatcaaa
     1321 aacgcttctt cacttcttta actttaaatt taacataaaa atcatacatc tgaaaattat
     1381 tggcgtgcgt gggaggtgtg tttgtgtggg aagtgccgcg tgccaaatta ccgttttttc
     1441 gaaagggttc gccaaatttt cgaggtgata aagatcgcac cttattggga aacattacaa
     1501 attttgagtc aaattgaagg aaatttattc agctatcagt gtttgataga agaatgtgcg
     1561 tatgacgtgg cagtcgcgcg aaaagctcat gtaaaagaac gcttcatgtg ttaccatact
     1621 tttggcccac cctgtataaa tcgtgaaaac ataattagat tataaaagta aaagtcgccc
     1681 ggtttcgatt atggttggca aaatctgagt aatcgctact tttttttgat tgcaattaaa
     1741 aataaattct gacacctttt aaaaacatgt accatgatat tttttaaacg atttcgcgac
     1801 ttggactaca tcttttgaac tccaatatta tcagttccga agtgtgatgt tgatgttaac
     1861 ataatgacat ttacaacgta ttctgatttc tttgaatatc ttctcgaact ctcgagacac
     1921 atctgttcag attccagttg acttgtcaca tttacgcatt tccctcctcc attcttttat
     1981 tttgtttcta tttcttgcgt aaatctgatt aaaatcgaga agtaggcgcg cattttctgt
     2041 cttcaccttt tgactataaa agtactctac aaatattgaa caacactttt taaaagttgt
     2101 agtagttatt ggccatcagg aattaggagg tcagttgcaa aatcatgcat cttttaggat
     2161 taaaagctta aatcttgctc attcatttcc tgtcttattt tgaagttttt ctctcttcca
     2221 acgcctccca ctgtgactac tatcatctat tattatcttg tagggattcc tcaatttcca
     2281 gacaccctca aactcggccc cgttccgatt cgaacggcgc gcctctagag gatccccggg
     2341 attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc
     2401 aggaggaccc ttggctagcg tcgacggatc ccgatgtcgg acacaattct tggtttcaac
     2461 aaatcaaacg tcgttttggc tgctggaatt gctggagccg ctttcctcgg ctactgcatt
     2521 tacttcgatc ataagagaat caacgctcca gactacaagg acaagattag gcaaagtcag
     2581 tgttttaaca acatatttcc ttcggatttt tatctaaaaa caacttattt tctttcagag
     2641 agacgtgccc aggctgggga tcctatggag aaaagtttcg tgataactga tccacggctg
     2701 ccagacaatc ccatcatctt cgcatccgat ggcttcctgg agctgaccga gtattccaga
     2761 gaggagatcc tgggccgcaa tggccgcttt ctgcagggac cagagacaga ccaggccaca
     2821 gtgcagaaga ttcgcgatgc cattagagat cagcgcgaga ttaccgtgca gctgataaac
     2881 tacacaaaaa gcgggaagaa attctggaac ctcctgcacc tccagcccat gagggaccag
     2941 aagggtgagc tccagtattt catcggagtg cagctggatg gataaagatc cccgagatct
     3001 tcagcgctca gcattcgtag aattgtaagt ttaaactttc tcatactaac taacgcgctc
     3061 tatttaaatt ttcagaattc gctgtctcat cctactttca cctagttaac tgcttgtctt
     3121 aaaatctatg cttctcttta gtatctaaaa ttttcctaga agcttacaag tatataaatg
     3181 gtctcttctc aataaaggtt gtatatttat tcatcttatt gaatctgcca tttcctcgtt
     3241 tttgcgagtt tatatacctt ccaattttct ttctattgta ttttcaactt ctaattttaa
     3301 ttcagggaaa ctgcttcaac gcatcggtac catggtctcc aagggagagg agctcttcac
     3361 cggagtcgtc ccaatcctcg tcgagctcga cggagacgtc aacggacaca agttctccgt
     3421 ccgtggagag ggagagggag acgccaccaa cggaaagctc accctcaagt tcatctgcac
     3481 caccggaaag ctcccagtcc catggccaac cctcgtcacc accctctccc acggagtcca
     3541 gtgcttcgcc cgttacccag accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3601 agagggatac gtccaggagc gtaccatctt cttcaaggac gacggaacct acaagacccg
     3661 tgccgaggtc aagttcgagg gagacaccct cgtcaaccgt atcgagctca agggagtcga
     3721 cttcaaggag gacggaaaca tcctcggaca caagctcgag tacaacttca actcccacaa
     3781 catctacatc atggccgtca agcagaagaa cggaatcaag gtcaacttca agatccgtca
     3841 caacgtcgag gacggatccg tccagctcgc cgaccactac cagcagaaca ccccaatcgg
     3901 agacggacca gtcctcctcc cagactccca ctacctctcc acccagtccg tcctctccaa
     3961 ggacccaaac gagaagcgtg accacatggt cctcctcgag ttccgtaccg ccgccggaat
     4021 caccctcgga atggacgagc tctacaagtg ataagatatc acctcccaca acgaggatta
     4081 cactatcgtt gagcaatatg aaagagctga agggcggcac tcgacaggtg gcatggatga
     4141 attgtataag taggagctcc cgagatcttc agcgctcagc attcgtagaa ttgtaagttt
     4201 aaactttctc atactaacta acgcgctcta tttaaatttt cagaattcca actgagcgcc
     4261 ggtcgctacc attaccaact tgtctggtgt caaaaataat aggggccgct gtcatcagag
     4321 taagtttaaa ctgagttcta ctaactaacg agtaatattt aaattttcag catctcgcgc
     4381 ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct
     4441 gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt
     4501 agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc
     4561 gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa
     4621 aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga
     4681 aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga
     4741 ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt
     4801 gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt
     4861 ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt
     4921 tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt
     4981 gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct
     5041 atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat aactgtttcc
     5101 tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     5161 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     5221 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     5281 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     5341 cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat
     5401 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5461 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5521 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5581 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5641 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5701 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5761 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5821 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5881 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5941 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6001 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6061 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6121 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6181 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6241 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6301 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6361 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6421 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6481 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6541 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6601 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6661 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6721 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6781 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6841 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6901 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6961 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7021 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7081 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7141 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7201 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7261 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7321 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7381 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7441 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7501 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7561 aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat
     7621 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
     7681 tcacaacgat ggatacgcta acaacttgga aatgaaat
//
