LOCUS       sequence-422142-        9822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of base editor RVR-A3A-N57G in mammalian cells for base 
            editing, along with non-fused EGFP fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, 
            Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao
            X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
  TITLE     Multiplexed base editing through Cas12a variant-mediated cytosine 
            and adenine base editors.
  JOURNAL   Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8.
  PUBMED    36323848
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-2"; volume: "5(1):1163. doi"; pages: " 
            10.1038/s42003-022-04152-8"
COMMENT     Sequence Label: pCMV-RVR-A3A-N57G
FEATURES             Location/Qualifiers
     source          1..9822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      69..78
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             75..671
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHGQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             4410..4430
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4443..4691
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4704..4724
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4731..4784
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4785..5033
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5034..5054
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5055..5111
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5112..5360
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5361..5381
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5382..5435
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5436..5684
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5697..5717
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5727..5786
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             join(5787..5789,5790..6503)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5787..5789,5790..6500)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5829..5850)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6090..6109)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6437..6458
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6543..6560)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6549..6773
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6844..6860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6844..6860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6857..6879)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6868..6884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6892..6898,6899..6916,6917..6922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6937..6958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7075..7092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7246..7834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7326..7345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8005..8796,8797..8865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8628..8647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8866..8970)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9049..9068)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9240..9619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9620..9822,1)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9774..9794
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagag atccgcggcc gctaatacga ctcactatag
       61 ggagagccgc caccatggaa gccagcccag catccgggcc cagacacttg atggatccac
      121 acatattcac ttccaacttt aacaatggca ttggaaggca taagacctac ctgtgctacg
      181 aagtggagcg cctggacaat ggcacctcgg tcaagatgga ccagcacagg ggctttctac
      241 acggccaggc taagaatctt ctctgtggct tttacggccg ccatgcggag ctgcgcttct
      301 tggacctggt tccttctttg cagttggacc cggcccagat ctacagggtc acttggttca
      361 tctcctggag cccctgcttc tcctggggct gtgccgggga agtgcgtgcg ttccttcagg
      421 agaacacaca cgtgagactg cgtatcttcg ctgcccgcat ctatgattac gaccccctat
      481 ataaggaggc actgcaaatg ctgcgggatg ctggggccca agtctccatc atgacctacg
      541 atgaatttaa gcactgctgg gacacctttg tggaccacca gggatgtccc ttccagccct
      601 gggatggact agatgagcac agccaagccc tgagtgggag gctgcgggcc attctccaga
      661 atcagggaaa cagcggcagc gagactcccg ggacctcaga gtccgccaca cccgaaagta
      721 tgtcaaagct ggagaaattc accaactgtt atagcctgtc taagaccctg cgcttcaagg
      781 caatcccagt gggcaagaca caagagaaca ttgacaacaa acggctcctg gtggaggatg
      841 agaagagggc tgaagattac aagggcgtta agaagctgct ggataggtac tatctgtcat
      901 tcatcaacga tgtcctccac agtatcaagc tgaagaatct gaacaattac atttctctgt
      961 tccggaagaa gacacggacc gagaaggaga acaaagagct ggagaatctg gagatcaacc
     1021 tgaggaaaga aatagctaag gctttcaaag ggaacgaggg ttacaagtcc ctgttcaaga
     1081 aagacattat cgagactatt ctgcctgagt tcctggacga taaagatgag atcgccctcg
     1141 tcaattcctt caatgggttt accacagcct ttaccggctt cttcgacaat agagagaata
     1201 tgttctctga agaggccaaa tccactagca tcgcctttcg ctgcataaac gagaacctga
     1261 ctaggtacat cagcaatatg gacatctttg agaaagtcga tgccatattc gacaaacatg
     1321 aggtgcagga gattaaggag aagatcctga actcagatta cgatgtcgaa gatttcttcg
     1381 agggagagtt cttcaacttc gtgctcacac aagagggcat tgatgtgtac aatgcaatca
     1441 ttggagggtt cgtgacagag agtggcgaga agataaaggg cctgaacgag tatatcaacc
     1501 tctacaacca gaaaaccaag cagaaactgc ctaagttcaa gccactgtac aaacaagtgc
     1561 tctcagatag ggaaagcctg agcttctacg gtgaagggta tacatcagat gaagaagtgc
     1621 tcgaagtgtt ccgcaacacc ctcaataaga acagtgaaat cttctcttca atcaagaagc
     1681 tggagaaact gttcaagaat ttcgatgagt actcctctgc cggaatcttt gtgaagaatg
     1741 gccctgcaat atccactatt agcaaagaca tctttggcga gtggaacgtt atcagggata
     1801 agtggaatgc cgagtacgat gatattcatc tcaagaagaa agccgtggtt acagagaaat
     1861 acgaggatga tagacgcaag agctttaaga agattggtag cttctctctc gaacagctgc
     1921 aggagtacgc cgacgctgac ctgtcagtcg tggagaaact caaggagatc ataatccaga
     1981 aggtggatga aatctacaaa gtgtatggaa gctctgagaa actcttcgat gcagactttg
     2041 ttctggagaa gagtctgaag aagaacgacg cagtggttgc tatcatgaag gacctgctgg
     2101 attctgttaa gtctttcgag aattacatta aggcattctt tggtgaaggg aaggagacaa
     2161 atagggacga gagcttctat ggcgactttg ttctggccta cgacatcctc ctcaaggttg
     2221 accacatcta tgacgctata cggaattacg ttacccagaa gccctatagc aaagacaagt
     2281 tcaagctgta tttccagaat ccacagttta tgagggggtg ggataaagac gttgaaacag
     2341 atcgcagggc cactatcctg cggtacggca gcaaatacta tctggctatc atggataaga
     2401 agtacgccaa atgcctccag aagatcgaca aggacgacgt gaacggtaac tacgagaaga
     2461 tcaattacaa gctcctgcca ggacctaaca agatgctgcc caaggtgttc ttctccaaga
     2521 aatggatggc ctactataac ccaagcgagg acattcagaa gatatacaag aatgggacat
     2581 tcaagaaggg cgatatgttc aacctcaacg actgccacaa gctgattgat ttcttcaagg
     2641 atagcatttc tcgctatccc aagtggtcta atgcatacga tttcaacttc agcgagactg
     2701 agaagtacaa agacatcgct ggcttctacc gggaggtgga agagcaaggc tataaggtgt
     2761 cattcgaatc cgcttctaag aaggaagtgg ataagctcgt ggaagagggt aagctgtaca
     2821 tgttccagat atacaacaaa gacttcagcg ataagagcca cggcactcca aacctccata
     2881 ctatgtattt caagctgctg tttgacgaga acaaccacgg acagattagg ctgtcaggag
     2941 gcgcagaact cttcatgcgc agagcttcac tgaagaagga ggaactcgtt gtccacccag
     3001 ccaatagccc tatagccaat aagaatccag acaatcctaa gaaaaccact actctgtctt
     3061 acgatgtgta taaggataag agattctctg aagatcagta cgaactgcac atacccattg
     3121 ccattaacaa gtgccctaag aacatcttca agattaacac agaggttaga gtgctcctga
     3181 aacacgacga taacccttat gttataggca ttgctcgcgg agagagaaac ctgctgtaca
     3241 tcgtcgtggt ggacggcaaa ggcaacatcg tggaacagta cagtctcaat gaaatcatta
     3301 acaatttcaa cggaatccgc attaagaccg actaccattc tctcctcgac aagaaggaga
     3361 aagaaaggtt cgaagcaaga cagaattgga caagtataga gaatatcaaa gaactgaagg
     3421 ctgggtacat ctctcaggtt gtgcacaaga tatgtgagct ggtggagaag tacgacgctg
     3481 ttatcgccct cgcggacctg aatagcggct tcaagaactc cagggtgaag gtggagaagc
     3541 aggtgtatca gaagttcgag aagatgctga tcgacaagct caactatatg gtggacaaga
     3601 aatccaatcc ttgcgctact ggtggagccc tgaagggcta tcaaatcacc aataagttcg
     3661 aatctttcaa gtctatgagc acccagaatg gcttcatctt ctacataccc gcatggctga
     3721 catccaagat tgatccctct accggatttg ttaatctgct caagactaag tacacctcta
     3781 ttgctgactc aaagaagttc atatcatcat ttgaccgcat catgtacgtg ccagaagagg
     3841 acctgttcga gtttgccctg gattacaaga atttctctcg gactgacgcc gactacatca
     3901 agaagtggaa gctctactct tatggtaatc ggattcgcat attccgcaat cccaagaaga
     3961 ataacgtgtt cgattgggag gaagtttgcc tcaccagcgc ttacaaggag ctgttcaata
     4021 agtatgggat taactaccag cagggcgaca taagagccct gctgtgcgaa caatctgata
     4081 aggcattcta ttcctctttc atggcactga tgtcactgat gctgcaaatg cgcaattcca
     4141 tcaccggaag aacagacgtg gcctttctga tctctcctgt caagaactca gatggcatct
     4201 tctacgattc ccgcaactat gaagcacagg agaatgctat cctgcctaag aatgccgatg
     4261 caaatggagc ctataacatc gccagaaagg tcctctgggc cataggacaa ttcaagaaag
     4321 ctgaagatga gaagctggac aaggtgaaga tcgccatttc aaacaaagag tggctcgaat
     4381 atgctcagac ctcagtgaag catggatcac ccaagaagaa acggaaagtg tctggtggtt
     4441 ctactaatct gtcagatatt attgaaaagg agaccggtaa gcaactggtt atccaggaat
     4501 ccatcctcat gctcccagag gaggtggaag aagtcattgg gaacaagccg gaaagcgata
     4561 tactcgtgca caccgcctac gacgagagca ccgacgagaa tgtcatgctt ctgactagcg
     4621 acgcccctga atacaagcct tgggctctgg tcatacagga tagcaacggt gagaacaaga
     4681 ttaagatgct ctctggtggt tctcccaaga agaagaggaa agtcgggccc gagggcagag
     4741 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaactaat ctgtcagata
     4801 ttattgaaaa ggagaccggt aagcaactgg ttatccagga atccatcctc atgctcccag
     4861 aggaggtgga agaagtcatt gggaacaagc cggaaagcga tatactcgtg cacaccgcct
     4921 acgacgagag caccgacgag aatgtcatgc ttctgactag cgacgcccct gaatacaagc
     4981 cttgggctct ggtcatacag gatagcaacg gtgagaacaa gattaagatg ctccccaaga
     5041 agaagaggaa agtcgctact aacttcagcc tgctgaagca ggctggagac gtggaggaga
     5101 accctggacc taccaacctg tccgatatca ttgagaaaga gaccggcaaa cagctggtga
     5161 tccaggagag catcctgatg ctgcccgaag aggtggagga agtgatcggc aacaagcccg
     5221 agtccgacat cctggtgcac acagcctatg atgaatccac cgacgagaac gtgatgctgc
     5281 tgacctccga tgctcccgag tataaaccct gggcactggt gatccaggac tctaatggag
     5341 agaacaagat caagatgctg cccaagaaga agaggaaagt cgaaggacgg ggctccctcc
     5401 tgacctgtgg cgatgtggaa gagaaccccg gccccacaaa cctcagtgac attatcgaga
     5461 aggaaacagg aaaacagctc gtcattcaag aatctattct tatgttgcct gaggaagtcg
     5521 aagaggttat tggcaataaa cctgaatctg atattcttgt ccataccgct tacgatgagt
     5581 ccacagatga aaatgttatg ctgctcacat ctgacgcacc agagtacaaa ccatgggcgc
     5641 tcgttattca agattccaac ggcgaaaaca aaatcaaaat gctttctggt ggttctccta
     5701 aaaaaaaaag aaaggtgggc tcgggccagt gtactaatta tgctctcttg aaattggctg
     5761 gagatgttga gagcaaccca ggtcccgtga gcaagggcga ggagctgttc accggggtgg
     5821 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     5881 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     5941 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     6001 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     6061 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     6121 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     6181 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     6241 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     6301 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     6361 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     6421 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     6481 gcatggacga gctgtacaag taaatcatca ccatcaccat tgagtttaaa cccgctgatc
     6541 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6601 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6661 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6721 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6781 ggcggaaaga accagctggg gctcgatacc gtcgacctct agctagagct tggcgtaatc
     6841 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6901 agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac tcacattaat
     6961 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7021 aatcggccaa cgcgcgggaa gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7681 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7921 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7981 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8041 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8101 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8161 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8221 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8281 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8341 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8401 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8461 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8521 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8581 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8641 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8701 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8761 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8821 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8881 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8941 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9001 cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc
     9061 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     9121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     9181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     9241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     9301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     9361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     9421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     9481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     9541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     9601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     9661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     9721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     9781 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gc
//
