LOCUS       sequence-422145-       16331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination vector Expressing nCas9 and RT fusion protein and 
            carrying Gateway recombination sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16331)
  AUTHORS   Gupta A, Liu B, Raza S, Chen QJ, Yang B
  TITLE     Modularly assembled multiplex prime editors for simultaneous editing
            of agronomically important genes in rice.
  JOURNAL   Plant Commun. 2023 Oct 26:100741. doi: 10.1016/j.xplc.2023.100741.
  PUBMED    37897041
REFERENCE   2  (bases 1 to 16331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2023 Oct 26:100741. doi: 10.1016/j.xplc.2023.100741."
COMMENT     Sequence Label: pG3H-PE3max-attR1R2
FEATURES             Location/Qualifiers
     source          1..16331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    59..183
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(208..213,214..231,232..238)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             594..899
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     778..797
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(940..1064)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3307..3327
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             7341..7349
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7486..7506
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9607..9627
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9637..9663
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     protein_bind    10356..10380
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    10653..10673
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10701..10717)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10701..10717)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10714..10736)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10725..10741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10749..10755,10756..10773,10774..10779))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10794..10815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11006..11682
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11619..11639
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11749..12774
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    12815..12989
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13067..13091
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    complement(13379..13399)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             13671..14465
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      14521..14607
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      14699..14726
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      14832..15420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15321..15340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      15535..16145
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      15622..16153
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aagctagaga cggggatcac
       61 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
      121 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
      181 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
      241 tggattttga gttaggatcg atccggctta ctaaaagcca gataacagta tgcgtatttg
      301 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
      361 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
      421 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
      481 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
      541 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
      601 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
      661 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
      721 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
      781 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
      841 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
      901 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
      961 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     1021 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgcttaa gtttgtgttt
     1081 ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga
     1141 tctcattata atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct
     1201 cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga
     1261 taactagtgc agcgtgaccc ggtcgtgccc ctcactagag ataatgagca ttgcatgtct
     1321 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
     1381 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
     1441 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
     1501 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
     1561 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
     1621 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
     1681 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
     1741 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
     1801 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
     1861 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
     1921 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     1981 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     2041 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
     2101 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
     2161 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     2221 acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc ccctctctac
     2281 cttctctaga tcggcgttcc ggtccatgct tagggcccgg tagttctact tctgttcatg
     2341 tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga
     2401 cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg
     2461 ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca
     2521 tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt
     2581 catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt
     2641 ctagatcgga gtacaattct gtttcaaact acctggtgga tttattaatt ttggatctgt
     2701 atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc
     2761 taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt
     2821 tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt
     2881 agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca
     2941 tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca
     3001 tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg
     3061 ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc
     3121 ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct
     3181 tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg
     3241 tgttacttct gcaggtacct aggcctctag atgaagagga cagccgatgg cagcgagttc
     3301 gagagcccta agaagaagag gaaggtggac aagaagtact cgatcggcct cgatattggg
     3361 actaactctg ttggctgggc cgtgatcacc gacgagtaca aggtgccctc aaagaagttc
     3421 aaggtcctgg gcaacaccga tcggcattcc atcaagaaga atctcattgg cgctctcctg
     3481 ttcgacagcg gcgagacggc tgaggctacg cggctcaagc gcaccgcccg caggcggtac
     3541 acgcgcagga agaatcgcat ctgctacctg caggagattt tctccaacga gatggcgaag
     3601 gttgacgatt ctttcttcca caggctggag gagtcattcc tcgtggagga ggataagaag
     3661 cacgagcggc atccaatctt cggcaacatt gtcgacgagg ttgcctacca cgagaagtac
     3721 cctacgatct accatctgcg gaagaagctc gtggactcca cagataaggc ggacctccgc
     3781 ctgatctacc tcgctctggc ccacatgatt aagttcaggg gccatttcct gatcgagggg
     3841 gatctcaacc cggacaatag cgatgttgac aagctgttca tccagctcgt gcagacgtac
     3901 aaccagctct tcgaggagaa ccccattaat gcgtcaggcg tcgacgcgaa ggctatcctg
     3961 tccgctaggc tctcgaagtc tcggaagctc gagaacctga tcgcccagct gccgggcgag
     4021 aagaagaacg gcctgttcgg gaatctcatt gcgctcagcc tggggctcac gcccaacttc
     4081 aagtcgaatt tcgatctcgc tgaggacgcc aagctgcagc tctccaagga cacatacgac
     4141 gatgacctgg ataacctcct ggcccagatc ggcgatcagt acgcggacct gttcctcgct
     4201 gccaagaatc tgtcggacgc catcctcctg tctgatattc tcagggtgaa caccgagatt
     4261 acgaaggctc cgctctcagc ctccatgatc aagcgctacg acgagcacca tcaggatctg
     4321 accctcctga aggcgctggt caggcagcag ctccccgaga agtacaagga gatcttcttc
     4381 gatcagtcga agaacggcta cgctgggtac attgacggcg gggcctctca ggaggagttc
     4441 tacaagttca tcaagccgat tctggagaag atggacggca cggaggagct gctggtgaag
     4501 ctcaagcgcg aggacctcct gaggaagcag cggacattcg ataacggcag catcccacac
     4561 cagattcatc tcggggagct gcacgctatc ctgaggaggc aggaggactt ctaccctttc
     4621 ctcaaggata accgcgagaa gatcgagaag attctgactt tcaggatccc gtactacgtc
     4681 ggcccactcg ctaggggcaa ctcccgcttc gcttggatga cccgcaagtc agaggagacg
     4741 atcacgccgt ggaacttcga ggaggtggtc gacaagggcg ctagcgctca gtcgttcatc
     4801 gagaggatga cgaatttcga caagaacctg ccaaatgaga aggtgctccc taagcactcg
     4861 ctcctgtacg agtacttcac agtctacaac gagctgacta aggtgaagta tgtgaccgag
     4921 ggcatgagga agccggcttt cctgtctggg gagcagaaga aggccatcgt ggacctcctg
     4981 ttcaagacca accggaaggt cacggttaag cagctcaagg aggactactt caagaagatt
     5041 gagtgcttcg attcggtcga gatctctggc gttgaggacc gcttcaacgc ctccctgggg
     5101 acctaccacg atctcctgaa gatcattaag gataaggact tcctggacaa cgaggagaat
     5161 gaggatatcc tcgaggacat tgtgctgaca ctcactctgt tcgaggaccg ggagatgatc
     5221 gaggagcgcc tgaagactta cgcccatctc ttcgatgaca aggtcatgaa gcagctcaag
     5281 aggaggaggt acaccggctg ggggaggctg agcaggaagc tcatcaacgg cattcgggac
     5341 aagcagtccg ggaagacgat cctcgacttc ctgaagagcg atggcttcgc gaaccgcaat
     5401 ttcatgcagc tgattcacga tgacagcctc acattcaagg aggatatcca gaaggctcag
     5461 gtgagcggcc agggggactc gctgcacgag catatcgcga acctcgctgg ctcgccagct
     5521 atcaagaagg ggattctgca gaccgtgaag gttgtggacg agctggtgaa ggtcatgggc
     5581 aggcacaagc ctgagaacat cgtcattgag atggcccggg agaatcagac cacgcagaag
     5641 ggccagaaga actcacgcga gaggatgaag aggatcgagg agggcattaa ggagctgggg
     5701 tcccagatcc tcaaggagca cccggtggag aacacgcagc tgcagaatga gaagctctac
     5761 ctgtactacc tccagaatgg ccgcgatatg tatgtggacc aggagctgga tattaacagg
     5821 ctcagcgatt acgacgtcga tgccatcgtt ccacagtcat tcctgaagga tgactccatt
     5881 gacaacaagg tcctcaccag gtcggacaag aaccggggca agtctgataa tgttccttca
     5941 gaggaggtcg ttaagaagat gaagaactac tggcgccagc tcctgaatgc caagctgatc
     6001 acgcagcgga agttcgataa cctcacaaag gctgagaggg gcgggctctc tgagctggac
     6061 aaggcgggct tcatcaagag gcagctggtc gagacacggc agatcactaa gcacgttgcg
     6121 cagattctcg actcacggat gaacactaag tacgatgaga atgacaagct gatccgcgag
     6181 gtgaaggtca tcaccctgaa gtcaaagctc gtctccgact tcaggaagga tttccagttc
     6241 tacaaggttc gggagatcaa caattaccac catgcccatg acgcgtacct gaacgcggtg
     6301 gtcggcacag ctctgatcaa gaagtaccca aagctcgaga gcgagttcgt gtacggggac
     6361 tacaaggttt acgatgtgag gaagatgatc gccaagtcgg agcaggagat tggcaaggct
     6421 accgccaagt acttcttcta ctctaacatt atgaatttct tcaagacaga gatcactctg
     6481 gccaatggcg agatccggaa gcgccccctc atcgagacga acggcgagac gggggagatc
     6541 gtgtgggaca agggcaggga tttcgcgacc gtcaggaagg ttctctccat gccacaagtg
     6601 aatatcgtca agaagacaga ggtccagact ggcgggttct ctaaggagtc aattctgcct
     6661 aagcggaaca gcgacaagct catcgcccgc aagaaggact gggatccgaa gaagtacggc
     6721 gggttcgaca gccccactgt ggcctactcg gtcctggttg tggcgaaggt tgagaagggc
     6781 aagtccaaga agctcaagag cgtgaaggag ctgctgggga tcacgattat ggagcgctcc
     6841 agcttcgaga agaacccgat cgatttcctg gaggcgaagg gctacaagga ggtgaagaag
     6901 gacctgatca ttaagctccc caagtactca ctcttcgagc tggagaacgg caggaagcgg
     6961 atgctggctt ccgctggcga gctgcagaag gggaacgagc tggctctgcc gtccaagtat
     7021 gtgaacttcc tctacctggc ctcccactac gagaagctca agggcagccc cgaggacaac
     7081 gagcagaagc agctgttcgt cgagcagcac aagcattacc tcgacgagat cattgagcag
     7141 atttccgagt tctccaagcg cgtgatcctg gccgacgcga atctggataa ggtcctctcc
     7201 gcgtacaaca agcaccgcga caagccaatc agggagcagg ctgagaatat cattcatctc
     7261 ttcaccctga cgaacctcgg cgcccctgct gctttcaagt acttcgacac aactatcgat
     7321 cgcaagaggt acacaagcac taaggaggtc ctggacgcga ccctcatcca ccagtcgatt
     7381 accggcctct acgagacgcg catcgacctg tctcagctcg ggggcgactc aggcggctca
     7441 tctggcgggt caaagcgcac agccgacggc tctgagttcg agagccctaa gaagaagcgc
     7501 aaggtgtcag gcggctcttc aggcggcagc accctgaaca ttgaggacga gtaccggctg
     7561 cacgagacga gcaaggagcc agacgtttcg ctcggcagca cttggctctc tgacttccca
     7621 caggcttggg ccgagactgg cggcatgggc ctggccgtgc gccaggctcc actgatcatc
     7681 cctctgaagg cgacctccac cccggtttct attaagcagt acccgatgag ccaggaggcc
     7741 aggctgggga tcaagccaca cattcagcgg ctgctggacc agggcatcct ggtgccatgc
     7801 cagtccccgt ggaatactcc gctcctgccg gtgaagaagc ctgggacaaa cgactacagg
     7861 ccggttcagg atctcaggga ggtgaacaag cgcgtggagg acatccatcc gacagtgccg
     7921 aacccgtaca atctgctgtc gggcctgcct ccgagccacc agtggtacac cgtcctggac
     7981 ctcaaggacg ctttcttctg cctgcggctg cacccgacgt ctcagccgct gttcgcgttc
     8041 gagtggcgcg acccagagat gggcatttcc ggccagctga cctggacacg cctaccccag
     8101 ggcttcaaga actccccgac tctcttcaac gaggctctcc accgggatct cgcggacttc
     8161 aggattcagc atcccgatct gatcctgctc cagtatgttg acgacctcct cctggccgcg
     8221 acgtcggagc tggactgcca gcagggcacc cgggcgctgc tgcagacact gggcaatctg
     8281 gggtaccgcg cctctgcgaa gaaggcgcag atctgccaga agcaagtgaa gtacctgggc
     8341 tacctcctga aggagggcca gcgctggctc actgaggcga ggaaggagac tgttatgggc
     8401 cagcccactc caaagactcc gaggcagctc agggagttcc tcggcaaggc tgggttctgc
     8461 cgcctgttca tccctgggtt cgctgagatg gctgcgccgc tctacccgct gactaagccg
     8521 gggacactgt tcaactgggg gccagaccag cagaaggcgt accaggagat taagcaggcg
     8581 ctgctgacgg ccccagcgct cggcctacca gacctgacga agccgttcga gctgttcgtt
     8641 gacgagaagc aggggtacgc gaagggcgtg ctgacacaga agctggggcc ttggcgccgc
     8701 ccggtcgcgt acctgtcgaa gaagctggac ccagtcgctg ctgggtggcc tccatgcctc
     8761 cggatggtcg ctgctattgc ggttctgacc aaggatgcgg ggaagctcac aatggggcag
     8821 cctctcgtga tcctggctcc acatgcggtg gaggcgctgg tgaagcagcc accggaccgg
     8881 tggctgtcga acgctcggat gacacactac caggcgctcc tcctcgatac agaccgggtt
     8941 cagttcgggc ctgtggttgc tctgaaccca gccacactgc tgccactccc tgaggagggc
     9001 ctccagcaca attgcctcga catcctggct gaggcgcacg gcacccgccc tgatctcacc
     9061 gaccagcctc tgccagatgc tgaccacacc tggtacacgg atgggtcctc gctgctgcag
     9121 gagggccaga ggaaggcggg cgccgccgtc accacagaga cagaggttat ttgggccaag
     9181 gccctaccgg ctggcaccag cgcccagcgc gctgagctga tcgcgctgac tcaggcgctg
     9241 aagatggccg aggggaagaa gctcaatgtt tacaccgact cgcggtacgc gttcgctaca
     9301 gctcacattc atggggagat ctaccgccgg cgcgggtggc tgacttcgga gggcaaggag
     9361 attaagaata aggacgagat cctggccctg ctcaaggcgc tgttcctgcc gaagcgcctc
     9421 tcaatcattc actgcccggg ccaccagaag ggccattcgg ccgaggctag gggcaatcgg
     9481 atggctgacc aggcggcgcg gaaggcggct atcaccgaga ctcccgatac atctaccctc
     9541 ctgatcgaga actcgagccc atctggcggc tctaagcgga ctgcggatgg gtctgagttc
     9601 gagtcaccaa agaagaagag gaaggtgggc tctggccctg ctgctaagcg cgtgaagctc
     9661 gattgagctc agagctttcg ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg
     9721 catcagtttc attgcgcaca caccagaatc ctactgagtt tgagtattat ggcattggga
     9781 aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt tattcggttt
     9841 tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga tatggtcctt
     9901 ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt gttgaatttg
     9961 aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc
    10021 taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta tgcttattca
    10081 ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga atacaagtat
    10141 gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca gaatccttgt
    10201 cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag ttgagtatga
    10261 aaatattttt taatgcattt tatgacttgc caattgattg acaacgaatt cgctacctta
    10321 ggaccgttat agttacgtta attaagaatt atcgaaccac tttgtacaag aaagctgggt
    10381 ctagctggcg cgccatataa gcttattacc ctgttatccc taatcgatcg atcggttaac
    10441 ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg tgggccccaa
    10501 atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat
    10561 tttgcgacaa catgtcgagg ctcagcagga cgaattcgta actataacgg tcctaaggta
    10621 gcgaattaat taacatgatt acgccaagct atcaactttg tatagaaaag ttgttagaat
    10681 tggcgcgaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    10741 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    10801 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    10861 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggctag
    10921 agcagcttgc caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
    10981 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
    11041 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
    11101 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
    11161 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
    11221 ttccaaccac gtcttcaaag caagtggatt gatgtgaaca tggtggagca cgacactctc
    11281 gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt
    11341 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
    11401 atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga
    11461 aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
    11521 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
    11581 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
    11641 tctatataag gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct
    11701 acaaatctat ctctctcgag ctttcgcaga tccggggggc aatgagatat gaaaaagcct
    11761 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
    11821 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
    11881 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
    11941 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggagtttagc
    12001 gagagcctga cctattgcat ctcccgccgt tcacagggtg tcacgttgca agacctgcct
    12061 gaaaccgaac tgcccgctgt tctacaaccg gtcgcggagg ctatggatgc gatcgctgcg
    12121 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
    12181 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
    12241 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
    12301 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
    12361 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
    12421 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
    12481 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccacg actccgggcg
    12541 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
    12601 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
    12661 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
    12721 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagaa atagagtaga
    12781 tgccgaccgg gatctgtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt
    12841 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
    12901 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
    12961 aaccaaaatc cagtactaaa atccagatcc cccgaattaa ttcggcgtta attcagtaca
    13021 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
    13081 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
    13141 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
    13201 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
    13261 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
    13321 ctgtgatcac cgcggtttca agcttgtatt caccggtgtt atggttaatt aacagcttca
    13381 actttattat acaaagttga taattcactg gcgcgccgta actataacgg tcctaaggta
    13441 gcgaaccgcg gctggcaaac agctattatg ggtattatgg gtctagtggg tttcaaaatc
    13501 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
    13561 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
    13621 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
    13681 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
    13741 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
    13801 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
    13861 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
    13921 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
    13981 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
    14041 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
    14101 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
    14161 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
    14221 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
    14281 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
    14341 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
    14401 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
    14461 tttagtacct aatctcgggg acgtctaact actaagcgag agtagggaac tgccaggcat
    14521 caaataaaac gaaaggctca gtcggaagac tgggcctttc gttttatctg ttgtttgtcg
    14581 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa
    14641 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aactaagcag
    14701 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgt tagatgcatg
    14761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    14821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    14881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    14941 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
    15001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    15061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    15121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    15181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    15241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    15301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    15361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    15421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    15481 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag atagctggtt
    15541 gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg
    15601 aagccgtgtg cgagacaccc cggccgccgg cgttgtggat acctcgcgga aaacttggcc
    15661 ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg
    15721 ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa
    15781 atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg
    15841 ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga
    15901 tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag
    15961 gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac
    16021 cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag
    16081 ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc
    16141 ctcccggtcg agtcatggtc tagcatggat ctcggggacg tcacccataa tacccataat
    16201 agctgtttgc cagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat
    16261 ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc
    16321 ttttctctta g
//
