LOCUS       sequence-422159-        7942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of cardiac troponin T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Fetterman KA, Blancard M, Lyra-Leite DM, Vanoye CG, Fonoudi H, Jouni
            M, DeKeyser JL, Lenny B, Sapkota Y, George AL Jr, Burridge PW
  TITLE     Independent compartmentalization of functional, metabolic, and 
            transcriptional maturation of hiPSC-derived cardiomyocytes.
  JOURNAL   Cell Rep. 2024 May 28;43(5):114160. doi: 
            10.1016/j.celrep.2024.114160. Epub 2024 Apr 27.
  PUBMED    38678564
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-05-28"; volume: "43(5):114160. doi"; pages: " 
            10.1016/j.celrep.2024.114160. Epub 2024 Apr 27"
COMMENT     Sequence Label: pAAVS1-Neo-TNNT2-Hygro
FEATURES             Location/Qualifiers
     source          1..7942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    796..821
                     /label=SA
                     /note="splice acceptor site"
     CDS             845..898
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             908..1708
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GTMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(968..987)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1578..1597
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1728..1745)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1734..1958
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             2611..3636
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2611..3636
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(3734..3753)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3799..3854
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3853..3872)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    4410..4939
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5085..5104)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5086..5104)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5111..5128)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5111..5127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5120..5142)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5269..5724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5356..5375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5566..5587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5750..5854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5855..5923,5924..6715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6073..6092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6886..7474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7375..7394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7628..7645
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7762..7783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7798..7803,7804..7821,7822..7828)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7836..7852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7841..7863
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7860..7876
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7860..7876
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7897..7915
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(7928..7942,1..789)
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 gcattctctc ccctgggcct gtgccgcttt ctgtctgcag cttgtggcct gggtcacctc
       61 tacggctggc ccagatcctt ccctgccgcc tccttcaggt tccgtcttcc tccactccct
      121 cttccccttg ctctctgctg tgttgctgcc caaggatgct ctttccggag cacttccttc
      181 tcggcgctgc accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg
      241 ggcatctctc ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct
      301 tctccttctg gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc
      361 tcccttgcgt cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac
      421 cccaaagtac cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct
      481 ggacaccccg ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc
      541 cctacccccc ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt
      601 ccaggggtcc gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc
      661 aggacagcat gtttgctgcc tccagggatc ctgtgtcccc gagctgggac caccttatat
      721 tcccagggcc ggttaatgtg gctctggttc tgggtacttt tatctgtccc ctccacccca
      781 cagtggggca agcttctgac ctcttctctt cctcccacag ggcctcgaga gatctggcag
      841 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag
      901 gctcgagggt accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
      961 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     1021 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     1081 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     1141 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     1201 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     1261 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     1321 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     1381 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     1441 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     1501 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     1561 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     1621 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     1681 ctatcgcctt cttgacgagt tcttctgagt ttaaacccgc tgatcagcct cgactgtgcc
     1741 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1801 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     1861 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     1921 caatagcagg catgctgggg atgcggtggg ctctatggac tagcggtggc ggcctcgaca
     1981 ttgattattg actagtgtca tggagaagac ccaccttgca gatgtcctca ctggggctgg
     2041 cagagccggc aacctgccca aggctgctca gtccattagg agccagtagc ctggaagatg
     2101 tctttacccc cagcatcagt tcaagtggag cagcacataa ctcttgccct ctgccttcca
     2161 agattctggt gctgagactt atggagtgtc ttggaggttg ccttctgccc cccaaccctg
     2221 ctcccagctg gccctcccag gcctgggttg ctggcctctg ctttatcagg attctcaaga
     2281 gggacagctg gtttatgttg catgactgtt ccctgcatat ctgctctggt tttaaatagc
     2341 ttatctgagc agctggagga ccacatgggc ttatatggcg tggggtacat gttcctgtag
     2401 ccttgtccct ggcacctgcc aaaatagcag ccaacacccc ccacccccac cgccatcccc
     2461 ctgccccacc cgtcccctgt cgcacattcc tccctccgca gggctggctc accaggcccc
     2521 agcccacatg cctgcttaaa gccctctcca tcctctgcct cacccagtcc ccgctgagac
     2581 tgagcagacg cctccaggat cgcggccgcc atgaaaaagc ctgaactcac cgcgacgtct
     2641 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     2701 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     2761 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     2821 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     2881 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     2941 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     3001 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     3061 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     3121 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     3181 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     3241 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     3301 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     3361 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     3421 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     3481 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     3541 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     3601 cgacgcccca gcactcgtcc gagggcaaag gaatagcctc gaggaagggc gaattcactc
     3661 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     3721 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     3781 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     3841 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     3901 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     3961 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     4021 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     4081 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     4141 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
     4201 ttggatccct cgagttaatt aaatctagaa gtcgacagta ctaagctttg acagaaaagc
     4261 cccatcctta ggcctcctcc ttcctagtct cctgatattg ggtctaaccc ccacctcctg
     4321 ttaggcagat tccttatctg gtgacacacc cccatttcct ggagccatct ctctccttgc
     4381 cagaacctct aaggtttgct tacgatggag ccagagagga tcctgggagg gagagcttgg
     4441 cagggggtgg gagggaaggg ggggatgcgt gacctgcccg gttctcagtg gccaccctgc
     4501 gctaccctct cccagaacct gagctgctct gacgcggctg tctggtgcgt ttcactgatc
     4561 ctggtgctgc agcttcctta cacttcccaa gaggagaagc agtttggaaa aacaaaatca
     4621 gaataagttg gtcctgagtt ctaactttgg ctcttcacct ttctagtccc caatttatat
     4681 tgttcctccg tgcgtcagtt ttacctgtga gataaggcca gtagccagcc ccgtcctggc
     4741 agggctgtgg tgaggagggg ggtgtccgtg tggaaaactc cctttgtgag aatggtgcgt
     4801 cctaggtgtt caccaggtcg tggccgcctc tactcccttt ctctttctcc atccttcttt
     4861 ccttaaagag tccccagtgc tatctgggac atattcctcc gcccagagca gggtcccgct
     4921 tccctaaggc cctgctctgg gcttctgggt ttgagtcctt ggcaagccca ggagaggcgc
     4981 tcaggcttcc ctgtccccct tcctcgtcca ccatctcatg cccctggctc tcctgcccct
     5041 tccctacagg ggttcctggc tctgctctag cgatcgccaa ttcgccctat agtgagtcgt
     5101 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     5161 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     5221 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     5281 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5341 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5401 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5461 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5521 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5581 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5641 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5701 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5761 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5821 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5881 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5941 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6001 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6061 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6121 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6181 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6241 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6301 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6361 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6421 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6481 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6541 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6601 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6661 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6721 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6781 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6841 gttccactga gcgtcaaacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6901 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6961 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7021 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7081 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7141 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7201 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7261 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7321 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7381 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7441 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7501 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7561 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7621 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7681 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     7741 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7801 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     7861 aggaaacagc tatgaccatg attacgccaa gctcgaaatt aaccctcact aaagggaaca
     7921 aaagctgtgc tttctctgac ca
//
