LOCUS       sequence-422173-        6485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Facilitates genomic integration of the control reporter without zinc
            finger binding sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Alcantar MA, English MA, Valeri JA, Collins JJ
  TITLE     A high-throughput synthetic biology approach for studying 
            combinatorial chromatin-based transcriptional regulation.
  JOURNAL   Mol Cell. 2024 Jun 20;84(12):2382-2396.e9. doi: 
            10.1016/j.molcel.2024.05.025.
  PUBMED    38906116
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05"; journalName: "Mol Cell"; date: 
            "2024-06-20- 20"; volume: "84"; issue: "12"; pages: "2382-2396.e9"
COMMENT     Sequence Label: pReporter_mNeonGreen
FEATURES             Location/Qualifiers
     source          1..6485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1842..2249
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2250..3344
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(4045..4836)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5266..5854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5755..5774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgaattc gcatctagat ggtagagcca caaacagccg gtacaagcaa cgatctccag
       61 gaccatctga atcatgcgcg gatgacacga actcacgacg gcgatcacag acattaaccc
      121 acagtacaga cactgcgaca acgtggcaat tcgtcgcaat accgtctcac tgaactggcc
      181 gataattgca gacgaacggc attccagatc cgccaggcgt gtatatatag cgtggatggc
      241 caggcaactt tagtgctgac acatacaggc atatatatat gtgtgcgacg acacatgatc
      301 atatggcatg catgtgctct gtatgtatat aaaactcttg ttttcttctt ttctctaaat
      361 attctttcct tatacattag gacctttgca gcataaatta ctatacttct atagacacac
      421 aaacacaaat acacacacta atctagatat taaatatggt ttcaaagggt gaagaggata
      481 acatggcaag tttgccggcc acacatgaac ttcacatttt cgggagcata aatggcgttg
      541 attttgacat ggtcggtcag gggactggga accccaacga cggatacgaa gaactcaact
      601 tgaaatctac caaaggtgat ctccaatttt caccgtggat tcttgttccg cacattgggt
      661 atggatttca tcagtatttg ccataccctg atggcatgtc acccttccag gccgctatgg
      721 tcgatggtag cggctaccag gtacaccgca caatgcagtt tgaagacggt gcatcactga
      781 ccgtgaacta ccgatatacc tatgagggtt cccatattaa aggagaagct caagtaaagg
      841 gaacgggatt cccggccgat ggtcccgtga tgactaactc cttgacagca gcagactggt
      901 gtcgctctaa gaagacttat cccaacgata aaaccattat aagtacattt aaatggagtt
      961 atacaacggg caacggtaag aggtacagaa gcacggcacg gaccacttac accttcgcta
     1021 agccgatggc cgcaaactac ttgaagaacc aaccgatgta cgtattccga aagacggaac
     1081 tgaaacatag caaaactgaa ctgaatttca aggagtggca gaaagcgttt actgatgtca
     1141 tgggtatgga cgagctgtat aagggatcct aactcgaggc caattggtgc ggcaattgat
     1201 aataacgaaa atgtctttta atgatctggg tataatgagg aattttccga acgtttttac
     1261 tttatatata tatatacatg taacatatat tctatacgct atagagaaag gaaatttttc
     1321 aattaaaaaa aaaatagaga aagagtttca cttcttgatt atcgctaaca ctaatggttg
     1381 aagtactgct actttaattt tatgctggaa atctgctcgt cagtggtgct cacactgacg
     1441 aatcatgtac agatcatacc gatgactgcc tggcgactca caactaagca agacagccgg
     1501 aaccagcgcc ggcgaacacc actgcatata tggcatatca caacagtcca cgtctcaagc
     1561 agttacagag atgttacgaa ccactagtgc actgcagtac atcgaggaga acttctagta
     1621 tatctacata cctaatatta ttgccttatt aaaaatggaa tcccaacaat tacatcaaaa
     1681 tccacattct cttcaaaatc aattgtcctg tacttccttg ttcatgtgtg ttcaaaaacg
     1741 ttatatttat aggataatta tactctattt ctcaacaagt aattggttgt ttggccgagc
     1801 ggtctaaggc gcctgattca agaaatatct tgaccgcagt taactgtggg aatactcagg
     1861 tatcgtaaga tgcaagagtt cgaatctctt agcaaccatt atttttttcc tcaacataac
     1921 gagaacacac aggggcgcta tcgcacagaa tcaaattcga tgactggaaa ttttttgtta
     1981 atttcagagg tcgcctgacg catatacctt tttcaactga aaaattggga gaaaaaggaa
     2041 aggtgagagc gccggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc
     2101 ttgcatcaca atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag
     2161 gtggttagca atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata
     2221 tatatatatt tcaaggatat accattctaa tgtctgcccc taagaagatc gtcgttttgc
     2281 caggtgacca cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt
     2341 ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta
     2401 tcgatgctac aggtgttcca cttccagatg aggcgctgga agcctccaag aaggctgatg
     2461 ccgttttgtt aggtgctgtg ggtggtccta aatggggaac cggtagtgtt agacctgaac
     2521 aaggtttact aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact
     2581 ttgcatccga ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg
     2641 acttcgttgt tgtcagagaa ttagtgggag gtatttactt tggtaagaga aaggaggacg
     2701 atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca
     2761 caagaatggc cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg
     2821 ataaagctaa tgttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca
     2881 agaacgaatt tcctacattg aaggttcaac atcaattgat tgattctgcc gccatgatcc
     2941 tagttaagaa cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata
     3001 tcatctccga tgaagcctcc gttatcccag gttccttggg tttgttgcca tctgcgtcct
     3061 tggcctcttt gccagacaag aacaccgcat ttggtttgta cgaaccatgc cacggttctg
     3121 ctccagattt gccaaagaat aaggtcaacc ctatcgccac tatcttgtct gctgcaatga
     3181 tgttgaaatt gtcattgaac ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa
     3241 aggttttgga tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag
     3301 tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc ttaacctgga ggacccttct
     3361 ctttagacta ttctactctt atgcacgtaa aaaattctag gaaatatgta ttaactagga
     3421 gtaaaataac cggctagtgg cattcatata gccgtctgtt tacatctaca tcacacattt
     3481 cgagtgtata tctcgcaacg ttggcgttaa ataggcagga gtactcgtat cgcatgtcgg
     3541 tgcgacacga aattacaaaa tggaatatgt tcatagggta gacgaaacta tatacgcaat
     3601 ctacatacat ttatcaagaa ggagaaaaag gaggatgtaa aggaatacag gtaagcaaat
     3661 tgatactaat ggctcaacgt gataaggaaa aagaattgca ctttaacatt aatattgaca
     3721 aggaggaggg caccacacaa aaagttaggt gtaacagaaa atcatgaaac tatgattcct
     3781 aatttatata ttggaggatt ttctctaaaa aaaaaaaaat acaacaaata aaaaacactc
     3841 aatgacctga ccatttgatg gagtttaagt caataccttc ttgaaccatt tcccataatg
     3901 gtgaaagttc cctcaagaat tttactctgt cagaaacggc cttaacgacg tagtcgacct
     3961 cctcttcagt actaaatcta ccaataccaa atctgatgga agaatgggct aatgcatcat
     4021 ccttacccag cgccgagcgg ccgcttattt gccgactacc ttggtgatct cgcctttcac
     4081 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
     4141 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
     4201 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
     4261 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
     4321 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
     4381 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
     4441 agccagatca atgtcgatcg tggctggctc gaagataccc gcaagaatgt cattgcgctg
     4501 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
     4561 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
     4621 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
     4681 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
     4741 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
     4801 ttgagtcgat acttcggcga tcaccgcttc cctcataatg tttaactttg ttttagggcg
     4861 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     4921 acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca
     4981 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
     5041 gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg cttatgtcca
     5101 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggtagcag
     5161 cgaagtcgag gcatttctgt cctggctggt catgaccaaa atcccttaac gtgagttttc
     5221 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5281 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5341 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5401 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5461 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5521 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5581 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5641 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5701 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5761 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5821 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5881 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5941 tgtggataac cgtgcggccg ccaataccca gaagaacagt aaaataaagc aaggtacgtg
     6001 aaattaatat ttttaaatgg ttctaaccga tgccgaagaa ctgcgcagtc cggttataac
     6061 gtctgacatg tccttttttg atttggaatc caaccactca agtgactctg ttcatttact
     6121 ttgcgaaaaa tatacccaca aattgcccat cgaaagtgaa tcgcaaacca ccttcagact
     6181 ggcaccgaca aagcaaagat tatacagaca gagtacttta tacgtaccgt taagtctcaa
     6241 gcaaagggtt ttcttattta ctgaacgggt aaagagtatc tgggccggct tgccaagatg
     6301 caaaccgaat aagtatttca aagttgcatt tgccttagcc gtcctgacac cattggctat
     6361 ttggatattt tatattgact ttcgtgtaca ttgatcacat cgactgttct attggcaaat
     6421 gaaccacggg cattgactat ttttcaggtt actactatat attatcatca cgggcaagga
     6481 ttgta
//
