LOCUS       sequence-422182-        4685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CombiGEM storage plasmid for ZF-1 CR fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Alcantar MA, English MA, Valeri JA, Collins JJ
  TITLE     A high-throughput synthetic biology approach for studying 
            combinatorial chromatin-based transcriptional regulation.
  JOURNAL   Mol Cell. 2024 Jun 20;84(12):2382-2396.e9. doi: 
            10.1016/j.molcel.2024.05.025.
  PUBMED    38906116
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05"; journalName: "Mol Cell"; date: 
            "2024-06-20- 20"; volume: "84"; issue: "12"; pages: "2382-2396.e9"
COMMENT     Sequence Label: pStorage_Ptet-1_ZF1
FEATURES             Location/Qualifiers
     source          1..4685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    92..110
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    131..149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             173..238
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             245..265
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        1184..1371
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             1372..2034
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     misc_feature    2249..2752
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(2769..3560)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3990..4578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4479..4498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgaatta catctagact gaactggccg ataattgcag acgaacgctg aagatctatt
       61 tttttctttt tttttttttg cggtataaaa atccctatca gtgatagaga ttttgttttt
      121 tttttttttt tccctatcag tgatagagaa tatcaagcta caaaaaggta tggattacaa
      181 agatcatgat ggtgattata aggaccatga tattgactac aaagatgacg acgacaaaat
      241 ggcccccaag aaaaagagga aggtaggtat ccacggtgtt ccgggtgggg gttctggttc
      301 tagacccggt gaacgtccgt ttcaatgtag aatctgcatg gcgaactttt ccagacaaga
      361 tcgtcttgat cgtcatacca ggacccacac tggcgaaaag ccctttcaat gtaggatatg
      421 tatggcgaac ttcagtcaga aagaacatct ggcggggcat ctgagaactc atactggtga
      481 aaagccattc cagtgccgta tctgcatggc aaatttttcc cgtagagata acttaaaccg
      541 tcatctgaaa acccatctga gaggttccgg aggttctggc ggtggaagtg gtggcggagg
      601 tagcggtggt ggcagcggtt cctgcagggg ttctgtcttc taataaataa agcaatcttg
      661 atgaggataa tgattttttt ttgaatatac ataaatacta ccgtttttct gctagatttt
      721 gtgatgacgt aaataagtac atattacttt ttaagccaag acaagattaa gcattaactt
      781 tacccttttc tttctaagtt tcaatattag ttatcactgt ttaaaagtta tggcgagaac
      841 gtcggcggtt aaaatatatt accctgaacg cctcttccag cgctcaacgg gtgtgcttcc
      901 cgttctgatg agtccgtgag gacgaaagcg tcccgggaat atcgcgtagc actttagact
      961 cttccttacg taatggatga ctcggcgcgt gatcttacgg ccgggcgaca cctctcatag
     1021 gtgtgcgtgg atcctaactt ttttggatcc gcaacggaat tcctgacgca caattgttgt
     1081 tgtttggctg agcagttaca gagatgttac gaaccactag tgcactgcag tacaaacaca
     1141 gtcctttccc gcaattttct ttttctatta ctcttggcct cctctagtac actctatatt
     1201 tttttatgcc tcggtaatga ttttcatttt tttttttcca cctagcggat gactcttttt
     1261 ttttcttagc gattggcatt atcacataat gaattataca ttatataaag taatgtgatt
     1321 tcttcgaaga atatactaaa aaatgagcag gcaagataaa cgaaggcaaa gatgacagag
     1381 cagaaagccc tagtaaagcg tattacaaat gaaaccaaga ttcagattgc gatctcttta
     1441 aagggtggtc ccctagcgat agagcactcg atcttcccag aaaaagaggc agaagcagta
     1501 gcagaacagg ccacacaatc gcaagtgatt aacgtccaca caggtatagg gtttctggac
     1561 catatgatac atgctctggc caagcattcc ggctggtcgc taatcgttga gtgcattggt
     1621 gacttacaca tagacgacca tcacaccact gaggactgcg ggattgctct cggtcaagct
     1681 tttaaagagg ccctaggggc cgtgcgtgga gtaaaaaggt ttggatcagg atttgcgcct
     1741 ttggatgagg cactttccag agcggtggtt gatctttcga acaggccgta cgcagttgtc
     1801 gaacttggtt tgcaaaggga gaaagtaggt gatctctctt gcgagatgat cccgcatttt
     1861 cttgaaagct ttgcagaggc tagcagaatt accctccacg ttgattgtct gcgaggcaag
     1921 aatgatcatc accgtagtga gagtgcgttc aaggctcttg cggttgccat aagagaagcc
     1981 acctcgccca atggtaccaa cgatgttccc tccaccaaag gtgttcttat gtagtgacac
     2041 cgattattta aagctgctgc atacgatata tatacatgtg tatatatgta tacctatgaa
     2101 tgtcagtaag tatgtatacg aacagtatga tactgaagat gacaaggtaa tgcatcattc
     2161 tatacgtgtc attctgaacg aggcgcgctt tccttttttc tttttgcttt ttcttttttt
     2221 ttctcttgaa ctcgacggat catagagtat cacgtgctat aaaaataatt ataatttaaa
     2281 ttttttaata taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa
     2341 tagtttttgt tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt
     2401 ttatcttgct cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag
     2461 aaaaccacac acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat
     2521 ctatttaatc tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa
     2581 ttttttaaac ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt
     2641 tactttctaa aatccaaata caaaacataa aaataaataa acacagagta aattcccaaa
     2701 ttattccatc attaaaagat acgaggcgcg tgtaagttac aggcaagcga tccgtcccga
     2761 gcggccgctt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
     2821 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
     2881 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     2941 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     3001 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     3061 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     3121 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     3181 atcgtggctg gctcgaagat acccgcaaga atgtcattgc gctgccattc tccaaattgc
     3241 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     3301 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     3361 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     3421 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     3481 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     3541 gcgatcaccg cttccctcat aatgtttaac tttgttttag ggcgactgcc ctgctgcgta
     3601 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     3661 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
     3721 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     3781 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc
     3841 atccgtttcc acggtgtgcg tcacccggca accttgggta gcagcgaagt cgaggcattt
     3901 ctgtcctggc tggtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3961 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4021 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4081 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4141 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4201 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4261 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4321 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4381 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4441 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4501 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4561 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4621 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg
     4681 gccgc
//
