LOCUS       sequence-422190-        9318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For circular RNA-mediated prime editor using LbCas12a-niCPE in 
            HEK293T cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Liang R, He Z, Zhao KT, Zhu H, Hu J, Liu G, Gao Q, Liu M, Zhang R, 
            Qiu JL, Gao C
  TITLE     Prime editing using CRISPR-Cas12a and circular RNAs in human cells.
  JOURNAL   Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02095-x.
  PUBMED    38200119
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02095-x."
COMMENT     Sequence Label: LbCas12a-niCPE
FEATURES             Location/Qualifiers
     source          1..9318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1183..4863
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMANSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VK"
     CDS             6553..6573
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6582..6599
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6622..6639)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6628..6852
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6923..6939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6923..6939)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6936..6958)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6947..6963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6971..6977,6978..6995,6996..7001))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7016..7037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7154..7171)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7325..7913)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7405..7424)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8084..8875,8876..8944))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8707..8726
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8945..9049)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9128..9147)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcaagctt
      721 gcgtccaact tcacccaatt tgtactggtg gacaatggtg gaacaggaga cgtgaccgtg
      781 gccccttcta acttcgccaa tggaatcgca gagtggattt cctcaaactc ccggtcacag
      841 gcatacaaag tgacgtgttc agtgaggcag tcctccgctc agaaccgcaa atacaccatt
      901 aaggttgaag tgccaaaagg cgcctggagg tcctacttga atatggagct taccattcca
      961 atttttgcca caaatagcga ttgtgagctg attgtgaagg ccatgcaggg cctcctgaag
     1021 gacgggaacc ccattccgag cgccatagca gcaaatagcg gaatttattc tagactgcag
     1081 tctggaggat ctagcggagg atcctctggc agcgagacac caggaacaag cgagtcagca
     1141 acaccagaga gcagtggcgg cagcagcggc ggcagcgagc tcatgagcaa gctggagaag
     1201 tttacaaact gctactccct gtctaagacc ctgaggttca aggccatccc tgtgggcaag
     1261 acccaggaga acatcgacaa taagcggctg ctggtggagg acgagaagag agccgaggat
     1321 tataagggcg tgaagaagct gctggatcgc tactatctgt cttttatcaa cgacgtgctg
     1381 cacagcatca agctgaagaa tctgaacaat tacatcagcc tgttccggaa gaaaaccaga
     1441 accgagaagg agaataagga gctggagaac ctggagatca atctgcggaa ggagatcgcc
     1501 aaggccttca agggcaacga gggctacaag tccctgttta agaaggatat catcgagaca
     1561 atcctgccag agttcctgga cgataaggac gagatcgccc tggtgaacag cttcaatggc
     1621 tttaccacag ccttcaccgg cttctttcgt aacagagaga atatgttttc cgaggaggcc
     1681 aagagcacat ccatcgcctt caggtgtatc aacgagaatc tgacccgcta catctctaat
     1741 atggacatct tcgagaaggt ggacgccatc tttgataagc acgaggtgca ggagatcaag
     1801 gagaagatcc tgaacagcga ctatgatgtg gaggatttct ttgagggcga gttctttaac
     1861 tttgtgctga cacaggaggg catcgacgtg tataacgcca tcatcggcgg cttcgtgacc
     1921 gagagcggcg agaagatcaa gggcctgaac gagtacatca acctgtataa tcagaaaacc
     1981 aagcagaagc tgcctaagtt taagccactg tataagcagg tgctgagcga tcgggagtct
     2041 ctgagcttct acggcgaggg ctatacatcc gatgaggagg tgctggaggt gtttagaaac
     2101 accctgaaca agaacagcga gatcttcagc tccatcaaga agctggagaa gctgttcaag
     2161 aattttgacg agtactctag cgccggcatc tttgtgaaga acggccccgc catcagcaca
     2221 atctccaagg atatcttcgg cgagtggaac gtgatccggg acaagtggaa tgccgagtat
     2281 gacgatatcc acctgaagaa gaaggccgtg gtgaccgaga agtacgagga cgatcggaga
     2341 aagtccttca agaagatcgg ctccttttct ctggagcagc tgcaggagta cgccgacgcc
     2401 gatctgtctg tggtggagaa gctgaaggag atcatcatcc agaaggtgga tgagatctac
     2461 aaggtgtatg gctcctctga gaagctgttc gacgccgatt ttgtgctgga gaagagcctg
     2521 aagaagaacg acgccgtggt ggccatcatg aaggacctgc tggattctgt gaagagcttc
     2581 gagaattaca tcaaggcctt ctttggcgag ggcaaggaga caaacaggga cgagtccttc
     2641 tatggcgatt ttgtgctggc ctacgacatc ctgctgaagg tggaccacat ctacgatgcc
     2701 atccgcaatt atgtgaccca gaagccctac tctaaggata agttcaagct gtattttcag
     2761 aaccctcagt tcatgggcgg ctgggacaag gataaggaga cagactatcg ggccaccatc
     2821 ctgagatacg gctccaagta ctatctggcc atcatggata agaagtacgc caagtgcctg
     2881 cagaagatcg acaaggacga tgtgaacggc aattacgaga agatcaacta taagctgctg
     2941 cccggcccta ataagatgct gccaaaggtg ttcttttcta agaagtggat ggcctactat
     3001 aaccccagcg aggacatcca gaagatctac aagaatggca cattcaagaa gggcgatatg
     3061 tttaacctga atgactgtca caagctgatc gacttcttta aggatagcat ctcccggtat
     3121 ccaaagtggt ccaatgccta cgatttcaac ttttctgaga cagagaagta taaggacatc
     3181 gccggctttt acagagaggt ggaggagcag ggctataagg tgagcttcga gtctgccagc
     3241 aagaaggagg tggataagct ggtggaggag ggcaagctgt atatgttcca gatctataac
     3301 aaggactttt ccgataagtc tcacggcaca cccaatctgc acaccatgta cttcaagctg
     3361 ctgtttgacg agaacaatca cggacagatc aggctgagcg gaggagcaga gctgttcatg
     3421 aggcgcgcct ccctgaagaa ggaggagctg gtggtgcacc cagccaactc ccctatcgcc
     3481 aacaagaatc cagataatcc caagaaaacc acaaccctgt cctacgacgt gtataaggat
     3541 aagaggtttt ctgaggacca gtacgagctg cacatcccaa tcgccatcaa taagtgcccc
     3601 aagaacatct tcaagatcaa tacagaggtg cgcgtgctgc tgaagcacga cgataacccc
     3661 tatgtgatcg gcatcgacag gggcgagcgc aatctgctgt atatcgtggt ggtggacggc
     3721 aagggcaaca tcgtggagca gtattccctg aacgagatca tcaacaactt caacggcatc
     3781 aggatcaaga cagattacca ctctctgctg gacaagaagg agaaggagag gttcgaggcc
     3841 cgccagaact ggacctccat cgagaatatc aaggagctga aggccggcta tatctctcag
     3901 gtggtgcaca agatctgcga gctggtggag aagtacgatg ccgtgatcgc cctggaggac
     3961 ctgaactctg gctttaagaa tagccgcgtg aaggtggaga agcaggtgta tcagaagttc
     4021 gagaagatgc tgatcgataa gctgaactac atggtggaca agaagtctaa tccttgtgca
     4081 acaggcggcg ccctgaaggg ctatcagatc accaataagt tcgagagctt taagtccatg
     4141 tctacccaga acggcttcat cttttacatc cctgcctggc tgacatccaa gatcgatcca
     4201 tctaccggct ttgtgaacct gctgaaaacc aagtatacca gcatcgccga ttccaagaag
     4261 ttcatcagct cctttgacag gatcatgtac gtgcccgagg aggatctgtt cgagtttgcc
     4321 ctggactata agaacttctc tcgcacagac gccgattaca tcaagaagtg gaagctgtac
     4381 tcctacggca accggatcag aatcttccgg aatcctaaga agaacaacgt gttcgactgg
     4441 gaggaggtgt gcctgaccag cgcctataag gagctgttca acaagtacgg catcaattat
     4501 cagcagggcg atatcagagc cctgctgtgc gagcagtccg acaaggcctt ctactctagc
     4561 tttatggccc tgatgagcct gatgctgcag atggcgaaca gcatcacagg ccgcaccgac
     4621 gtggattttc tgatcagccc tgtgaagaac tccgacggca tcttctacga tagccggaac
     4681 tatgaggccc aggagaatgc catcctgcca aagaacgccg acgccaatgg cgcctataac
     4741 atcgccagaa aggtgctgtg ggccatcggc cagttcaaga aggccgagga cgagaagctg
     4801 gataaggtga agatcgccat ctctaacaag gagtggctgg agtacgccca gaccagcgtg
     4861 aagtctggag gatctagcgg aggatcctct ggcagcgaga caccaggaac aagcgagtca
     4921 gcaacaccag agagcagtgg cggttcgtct ggaggttcac ccgggagcac cctaaatata
     4981 gaagatgagt atcggctaca tgagacctca aaagagccag atgtttctct agggtccaca
     5041 tggctgtctg attttcctca ggcctgggcg gaaaccgggg gcatgggact ggcagttcgc
     5101 caagctcctc tgatcatacc tctgaaagca acctctaccc ccgtgtccat aaaacaatac
     5161 cccatgtcac aagaagccag actggggatc aagccccaca tacagagact gttggaccag
     5221 ggaatactgg taccctgcca gtccccctgg aacacgcccc tgctacccgt taagaaacca
     5281 gggactaatg attataggcc tgtccaggat ctgagagaag tcaacaagcg ggtggaagac
     5341 atccacccca ccgtgcccaa cccttacaac ctcttgagcg ggctcccacc gtcccaccag
     5401 tggtacactg tgcttgattt aaaggatgcc tttttctgcc tgagactcca ccccaccagt
     5461 cagcctctct tcgcctttga gtggagagat ccagagatgg gaatctcagg acaattgacc
     5521 tggaccagac tcccacaggg tttcaaaaac agtcccaccc tgtttaatga ggcactgcac
     5581 agagacctag cagacttccg gatccagcac ccagacttga tcctgctaca gtacgtggat
     5641 gacttactgc tggccgccac ttctgagcta gactgccaac aaggtactcg ggccctgtta
     5701 caaaccctag ggaacctcgg gtatcgggcc tcggccaaga aagcccaaat ttgccagaaa
     5761 caggtcaagt atctggggta tcttctaaaa gagggtcaga gatggctgac tgaggccaga
     5821 aaagagactg tgatggggca gcctactccg aagacccctc gacaactaag ggagttccta
     5881 gggaaggcag gcttctgtcg cctcttcatc cctgggtttg cagaaatggc agcccccctg
     5941 taccctctca ccaaaccggg gactctgttt aattggggcc cagaccaaca aaaggcctat
     6001 caagaaatca agcaagctct tctaactgcc ccagccctgg ggttgccaga tttgactaag
     6061 ccctttgaac tctttgtcga cgagaagcag ggctacgcca aaggtgtcct aacgcaaaaa
     6121 ctgggacctt ggcgtcggcc ggtggcctac ctgtccaaaa agctagaccc agtagcagct
     6181 gggtggcccc cttgcctacg gatggtagca gccattgccg tactgacaaa ggatgcaggc
     6241 aagctaacca tgggacagcc actagtcatt ctggcccccc atgcagtaga ggcactagtc
     6301 aaacaacccc ccgaccgctg gctttccaac gcccggatga ctcactatca ggccttgctt
     6361 ttggacacgg accgggtcca gttcggaccg gtggtagccc tgaacccggc tacgctgctc
     6421 ccactgcctg aggaagggct gcaacacaac tgccttgata tcctggccga agcccacgga
     6481 acccgacccg acctaacgga ccagccgctc tctggcggct caaaaagaac cgccgacggc
     6541 agcgaattcg agcccaagaa gaagaggaaa gtctaaccgg tcatcatcac catcaccatt
     6601 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6661 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6721 aaaatgagaa aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6781 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6841 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg tcgacctcta
     6901 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6961 caattccaca caacatacga gccggaagca taaagtgtaa agcctagggt gcctaatgag
     7021 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7081 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7141 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7201 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7261 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7321 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7381 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7441 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7501 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7561 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7621 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7681 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7741 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7801 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7861 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7921 ctttgatctt ttctacgggg tctgacactc agtggaacga aaactcacgt taagggattt
     7981 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8041 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8101 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8161 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8221 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8281 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8341 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8401 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8461 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8521 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8581 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8641 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8701 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8761 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8821 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8881 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8941 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9001 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9061 gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcta
     9121 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     9181 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     9241 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     9301 gggccagata tacgcgtt
//
