LOCUS       sequence-422194-        8868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For circular RNA-mediated prime editor using rnLbCas12a-PE2 in 
            HEK293T cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Liang R, He Z, Zhao KT, Zhu H, Hu J, Liu G, Gao Q, Liu M, Zhang R, 
            Qiu JL, Gao C
  TITLE     Prime editing using CRISPR-Cas12a and circular RNAs in human cells.
  JOURNAL   Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02095-x.
  PUBMED    38200119
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02095-x."
COMMENT     Sequence Label: rnLbCas12a-PE2
FEATURES             Location/Qualifiers
     source          1..8868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             733..4413
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVAPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMANSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VK"
     CDS             6103..6123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6132..6149
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6172..6189)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6178..6402
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6473..6489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6473..6489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6486..6508)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6497..6513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6521..6527,6528..6545,6546..6551))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6566..6587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6704..6721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6875..7463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6955..6974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7634..8425,8426..8494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8257..8276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8495..8599)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8678..8697)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcaagctt
      721 tctagagagc tcatgagcaa gctggagaag tttacaaact gctactccct gtctaagacc
      781 ctgaggttca aggccatccc tgtgggcaag acccaggaga acatcgacaa taagcggctg
      841 ctggtggagg acgagaagag agccgaggat tataagggcg tgaagaagct gctggatcgc
      901 tactatctgt cttttatcaa cgacgtgctg cacagcatca agctgaagaa tctgaacaat
      961 tacatcagcc tgttccggaa gaaaaccaga accgagaagg agaataagga gctggagaac
     1021 ctggagatca atctgcggaa ggagatcgcc aaggccttca agggcaacga gggctacaag
     1081 tccctgttta agaaggatat catcgagaca atcctgccag agttcctgga cgataaggac
     1141 gagatcgccc tggtgaacag cttcaatggc tttaccacag ccttcaccgg cttctttcgt
     1201 aacagagaga atatgttttc cgaggaggcc aagagcacat ccatcgcctt caggtgtatc
     1261 aacgagaatc tgacccgcta catctctaat atggacatct tcgagaaggt ggacgccatc
     1321 tttgataagc acgaggtgca ggagatcaag gagaagatcc tgaacagcga ctatgatgtg
     1381 gaggatttct ttgagggcga gttctttaac tttgtgctga cacaggaggg catcgacgtg
     1441 tataacgcca tcatcggcgg cttcgtgacc gagagcggcg agaagatcaa gggcctgaac
     1501 gagtacatca acctgtataa tcagaaaacc aagcagaagc tgcctaagtt taagccactg
     1561 tataagcagg tgctgagcga tcgggagtct ctgagcttct acggcgaggg ctatacatcc
     1621 gatgaggagg tgctggaggt gtttagaaac accctgaaca agaacagcga gatcttcagc
     1681 tccatcaaga agctggagaa gctgttcaag aattttgacg agtactctag cgccggcatc
     1741 tttgtgaaga acggccccgc catcagcaca atctccaagg atatcttcgg cgagtggaac
     1801 gtgatccggg acaagtggaa tgccgagtat gacgatatcc acctgaagaa gaaggccgtg
     1861 gtgaccgaga agtacgagga cgatcggaga aagtccttca agaagatcgg ctccttttct
     1921 ctggagcagc tgcaggagta cgccgacgcc gatctgtctg tggtggagaa gctgaaggag
     1981 atcatcatcc agaaggtgga tgagatctac aaggtgtatg gctcctctga gaagctgttc
     2041 gacgccgatt ttgtgctgga gaagagcctg aagaagaacg acgccgtggt ggccatcatg
     2101 aaggacctgc tggattctgt gaagagcttc gagaattaca tcaaggcctt ctttggcgag
     2161 ggcaaggaga caaacaggga cgagtccttc tatggcgatt ttgtgctggc ctacgacatc
     2221 ctgctgaagg tggaccacat ctacgatgcc atccgcaatt atgtgaccca gaagccctac
     2281 tctaaggata agttcaagct gtattttcag aaccctcagt tcatgggcgg ctgggacaag
     2341 gataaggaga cagactatcg ggccaccatc ctgagatacg gctccaagta ctatctggcc
     2401 atcatggata agaagtacgc caagtgcctg cagaagatcg acaaggacga tgtgaacggc
     2461 aattacgaga agatcaacta taagctgctg cccggcccta ataagatgct gccaaaggtg
     2521 ttcttttcta agaagtggat ggcctactat aaccccagcg aggacatcca gaagatctac
     2581 aagaatggca cattcaagaa gggcgatatg tttaacctga atgactgtca caagctgatc
     2641 gacttcttta aggatagcat ctcccggtat ccaaagtggt ccaatgccta cgatttcaac
     2701 ttttctgaga cagagaagta taaggacatc gccggctttt acagagaggt ggaggagcag
     2761 ggctataagg tgagcttcga gtctgccagc aagaaggagg tggataagct ggtggaggag
     2821 ggcaagctgt atatgttcca gatctataac aaggactttt ccgataagtc tcacggcaca
     2881 cccaatctgc acaccatgta cttcaagctg ctgtttgacg agaacaatca cggacagatc
     2941 aggctgagcg gaggagcaga gctgttcatg aggcgcgcct ccctgaagaa ggaggagctg
     3001 gtggtggccc cagccaactc ccctatcgcc aacaagaatc cagataatcc caagaaaacc
     3061 acaaccctgt cctacgacgt gtataaggat aagaggtttt ctgaggacca gtacgagctg
     3121 cacatcccaa tcgccatcaa taagtgcccc aagaacatct tcaagatcaa tacagaggtg
     3181 cgcgtgctgc tgaagcacga cgataacccc tatgtgatcg gcatcgacag gggcgagcgc
     3241 aatctgctgt atatcgtggt ggtggacggc aagggcaaca tcgtggagca gtattccctg
     3301 aacgagatca tcaacaactt caacggcatc aggatcaaga cagattacca ctctctgctg
     3361 gacaagaagg agaaggagag gttcgaggcc cgccagaact ggacctccat cgagaatatc
     3421 aaggagctga aggccggcta tatctctcag gtggtgcaca agatctgcga gctggtggag
     3481 aagtacgatg ccgtgatcgc cctggaggac ctgaactctg gctttaagaa tagccgcgtg
     3541 aaggtggaga agcaggtgta tcagaagttc gagaagatgc tgatcgataa gctgaactac
     3601 atggtggaca agaagtctaa tccttgtgca acaggcggcg ccctgaaggg ctatcagatc
     3661 accaataagt tcgagagctt taagtccatg tctacccaga acggcttcat cttttacatc
     3721 cctgcctggc tgacatccaa gatcgatcca tctaccggct ttgtgaacct gctgaaaacc
     3781 aagtatacca gcatcgccga ttccaagaag ttcatcagct cctttgacag gatcatgtac
     3841 gtgcccgagg aggatctgtt cgagtttgcc ctggactata agaacttctc tcgcacagac
     3901 gccgattaca tcaagaagtg gaagctgtac tcctacggca accggatcag aatcttccgg
     3961 aatcctaaga agaacaacgt gttcgactgg gaggaggtgt gcctgaccag cgcctataag
     4021 gagctgttca acaagtacgg catcaattat cagcagggcg atatcagagc cctgctgtgc
     4081 gagcagtccg acaaggcctt ctactctagc tttatggccc tgatgagcct gatgctgcag
     4141 atggcgaaca gcatcacagg ccgcaccgac gtggattttc tgatcagccc tgtgaagaac
     4201 tccgacggca tcttctacga tagccggaac tatgaggccc aggagaatgc catcctgcca
     4261 aagaacgccg acgccaatgg cgcctataac atcgccagaa aggtgctgtg ggccatcggc
     4321 cagttcaaga aggccgagga cgagaagctg gataaggtga agatcgccat ctctaacaag
     4381 gagtggctgg agtacgccca gaccagcgtg aagtctggag gatctagcgg aggatcctct
     4441 ggcagcgaga caccaggaac aagcgagtca gcaacaccag agagcagtgg cggttcgtct
     4501 ggaggttcac ccgggagcac cctaaatata gaagatgagt atcggctaca tgagacctca
     4561 aaagagccag atgtttctct agggtccaca tggctgtctg attttcctca ggcctgggcg
     4621 gaaaccgggg gcatgggact ggcagttcgc caagctcctc tgatcatacc tctgaaagca
     4681 acctctaccc ccgtgtccat aaaacaatac cccatgtcac aagaagccag actggggatc
     4741 aagccccaca tacagagact gttggaccag ggaatactgg taccctgcca gtccccctgg
     4801 aacacgcccc tgctacccgt taagaaacca gggactaatg attataggcc tgtccaggat
     4861 ctgagagaag tcaacaagcg ggtggaagac atccacccca ccgtgcccaa cccttacaac
     4921 ctcttgagcg ggctcccacc gtcccaccag tggtacactg tgcttgattt aaaggatgcc
     4981 tttttctgcc tgagactcca ccccaccagt cagcctctct tcgcctttga gtggagagat
     5041 ccagagatgg gaatctcagg acaattgacc tggaccagac tcccacaggg tttcaaaaac
     5101 agtcccaccc tgtttaatga ggcactgcac agagacctag cagacttccg gatccagcac
     5161 ccagacttga tcctgctaca gtacgtggat gacttactgc tggccgccac ttctgagcta
     5221 gactgccaac aaggtactcg ggccctgtta caaaccctag ggaacctcgg gtatcgggcc
     5281 tcggccaaga aagcccaaat ttgccagaaa caggtcaagt atctggggta tcttctaaaa
     5341 gagggtcaga gatggctgac tgaggccaga aaagagactg tgatggggca gcctactccg
     5401 aagacccctc gacaactaag ggagttccta gggaaggcag gcttctgtcg cctcttcatc
     5461 cctgggtttg cagaaatggc agcccccctg taccctctca ccaaaccggg gactctgttt
     5521 aattggggcc cagaccaaca aaaggcctat caagaaatca agcaagctct tctaactgcc
     5581 ccagccctgg ggttgccaga tttgactaag ccctttgaac tctttgtcga cgagaagcag
     5641 ggctacgcca aaggtgtcct aacgcaaaaa ctgggacctt ggcgtcggcc ggtggcctac
     5701 ctgtccaaaa agctagaccc agtagcagct gggtggcccc cttgcctacg gatggtagca
     5761 gccattgccg tactgacaaa ggatgcaggc aagctaacca tgggacagcc actagtcatt
     5821 ctggcccccc atgcagtaga ggcactagtc aaacaacccc ccgaccgctg gctttccaac
     5881 gcccggatga ctcactatca ggccttgctt ttggacacgg accgggtcca gttcggaccg
     5941 gtggtagccc tgaacccggc tacgctgctc ccactgcctg aggaagggct gcaacacaac
     6001 tgccttgata tcctggccga agcccacgga acccgacccg acctaacgga ccagccgctc
     6061 tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa
     6121 gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
     6181 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     6241 aaggtgccac tcccactgtc ctttcctaat aaaatgagaa aattgcatcg cattgtctga
     6301 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6361 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     6421 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6481 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6541 taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6601 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6661 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6721 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6781 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6841 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6901 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6961 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7021 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7081 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7141 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7201 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7261 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7321 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7381 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7441 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacactc
     7501 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7561 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7621 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7681 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7741 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7801 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7861 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7921 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7981 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8041 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8101 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8161 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8221 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8281 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8341 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8401 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8461 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8521 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8581 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8641 gagatcgatc tcccgatccc ctagggtcta ctctcagtac aatctgctct gatgccgcat
     8701 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     8761 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     8821 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
