LOCUS       sequence-42220-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Dewaste V, Moreau C, De Smedt F, Bex F, De Smedt H, Wuytack F, 
            Missiaen L, Erneux C
  TITLE     The three isoenzymes of human inositol-1,4,5-trisphosphate 3-kinase 
            show specific intracellular localization but comparable Ca2+ 
            responses on transfection in COS-7 cells.
  JOURNAL   Biochem J. 2003 Aug 15;374(Pt 1):41-9.
  PUBMED    12747803
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "2003-08-15"; volume: "374(Pt 1)"; pages: "41-9"
COMMENT     Sequence Label: pcDNA3-IP3KA
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(254..273)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(255..273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(318..521)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(347..367)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggacctgtc ccccacgccg cttggccccg cgggcccggg gctcccccgc ggcggcggcc
       61 gcagcgaccg ccgcacagcg cgcctcgaag agcaggcgca gctccccgac actccggcgc
      121 ggggcccgct ccagccccgg gctgcagggc ctcgcgcccc ccggccgcgc catgcccgtt
      181 gggcccccgg gcagggtcat ttccggaatt cctgcagccc gggggatccg agctcggtac
      241 caagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta agcagtgggt
      301 tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg cctaccgccc
      361 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
      421 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
      481 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
      541 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
      601 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
      661 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
      721 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
      781 tt
//
