LOCUS       sequence-422219-        5267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Riboptoregulator circuit RoRH for blue-light-activated gene 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ranzani AT, Buchholz K, Blackholm M, Kopkin H, Moglich A
  TITLE     Induction of bacterial expression at the mRNA level by light.
  JOURNAL   Nucleic Acids Res. 2024 Aug 10:gkae678. doi: 10.1093/nar/gkae678.
  PUBMED    39126322
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Aug 10:gkae678. doi: 10.1093/nar/gkae678."
COMMENT     Sequence Label: pRoRH
FEATURES             Location/Qualifiers
     source          1..5267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     214..233
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1454..1483
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1536..1554)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1550..1597
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1651..1668)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             1869..1886
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1896..1913
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     terminator      2093..2179
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2271..2298
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(2499..3290)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3291..3382)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3430..4168)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(4369..5247)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
       61 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
      121 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
      181 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
      241 tagcggatcc taccttttta aaaaattcat ttgctaaacg cttcaaattc tcgtataatt
      301 acttttctcc atacccgttt tttgggctaa cccgccgaaa cctaaggaaa tacatagtat
      361 gaaagttaat cgtccggcag aacgtgccag ctttggtagc tttgttctgg atgcaggtag
      421 cgcacgtttt gttggtagtg atgaactggc actggttctg ggttttgcac cgggtgatgt
      481 tgttctgaca ccggcagttg ttctggcaca tctgcatcct gatgatcgtc tggaatggca
      541 ggcaggtctg cagcgttgtc tggcaaccgg tcgtccggtg gttgttaatc atctgctgct
      601 gaccgcagaa gcagaaccgc gtccggcaat gacaaccctg accgcactga ccgaacagga
      661 tcgtgtgcgt gcagttaccg gtgttattac cgatctgagc gatcgtgttc gtcgtgcaac
      721 cgaagcagaa attcgtcagg cagttcgtgc agcagcagca acccgtagcg aaattgatca
      781 ggcaaaaggt attgttatgg cagcctttga tgttgatgca gatcaggcat ttgcactgct
      841 gaaatggcat agcagccaga gcaatcgtaa actgcgtgat ctggccaccg gtatgattga
      901 aggtctggca gcagccaata gcgcactgcc gctgcgtcgt cgtctgagca ccgtttttac
      961 cgatatgggt tgtccggcac cgagcaccaa aggttggacc gttccggtga ccgatattgg
     1021 tctgcctccg accagcggtc tgattccgac agcactgctg cctggtattc tgacccgtgc
     1081 agcacatgat gcaagcgttg caattaccgt tgcagatgtt accgcaccgg atcagcctct
     1141 ggtttatgca aatcctgcat ttgaacgtct gaccggttat gcagcagccg aagttctggg
     1201 tcgtaattgt cgttttctgc aggcagaaag cggtgatccg catgaacgta gcgcaattcg
     1261 tagcgccatt gcaaatggtg atgcagtgac cacactgatt cgtaattttc gtcaggatgg
     1321 tcatgccttt tggaacgaat ttcatctgag tccggttcgt aatggtgcag gtcgtgttac
     1381 ccattacatt ggttatcagc tggatgtgac cgaacgtgtt gaacgtgatc agcagctgga
     1441 acagctggca ggcgaacaga aactgatttc tgaagaagat ctgtaagatc cggctgctaa
     1501 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     1561 ccttggggcc tctaaacggg tcttgagggg ttttttgctc ggccgcactc gagagcttgg
     1621 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
     1681 cggtctgata aaacagaatt tgccggtgtt gacataaata ccactggcgg tgatactgag
     1741 cacatcagca ggcccctcta gaaataattt tgtttaactt taaatagatc acctgctagc
     1801 cggttcagca gcgagccgta agcctcactg ccggaactga taggaggaga tataccatgg
     1861 gcagcagcca tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgg
     1921 gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcatga gatccctggc
     1981 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     2041 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     2101 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2161 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     2221 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     2281 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
     2341 caaatatgta tccgctcatg gacactgctt ccggtagtca ataaaccggt aaaccagcaa
     2401 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc atcgtggccg
     2461 gatcttgcgg cccctcggct tgaacgaatt gttagacatt atttgccgac taccttggtg
     2521 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     2581 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     2641 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     2701 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     2761 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     2821 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     2881 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     2941 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     3001 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     3061 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     3121 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     3181 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     3241 actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat actcttcctt
     3301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3361 tgtatttaga aaaataaaca aatagctagc tcactcggtc gctacgctcc gggcgtgaga
     3421 ctgcggcggg cgctgcggac acatacaaag ttacccacag attccgtgga taagcagggg
     3481 actaacatgt gaggcaaaac agcagggccg cgccggtggc gtttttccat aggctccgcc
     3541 ctcctgccag agttcacata aacagacgct tttccggtgc atctgtggga gccgtgaggc
     3601 tcaaccatga atctgacagt acgggcgaaa cccgacagga cttaaagatc cccaccgttt
     3661 ccggcgggtc gctccctctt gcgctctcct gttccgaccc tgccgtttac cggatacctg
     3721 ttccgccttt ctcccttacg ggaagtgtgg cgctttctca tagctcacac actggtatct
     3781 cggctcggtg taggtcgttc gctccaagct gggctgtaag caagaactcc ccgttcagcc
     3841 cgactgctgc gccttatccg gtaactgttc acttgagtcc aacccggaaa agcacggtaa
     3901 aacgccactg gcagcagcca ttggtaactg ggagttcgca gaggatttgt ttagctaaac
     3961 acgcggttgc tcttgaagtg tgcgccaaag tccggctaca ctggaaggac agatttggtt
     4021 gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt ccccaactga cttaaccttc
     4081 gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa
     4141 aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgctctaga tttcagtgca
     4201 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     4261 atgttagtca gccccgcatg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca
     4321 aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt
     4381 gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc
     4441 ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc
     4501 gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg
     4561 gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg
     4621 cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag
     4681 gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga
     4741 ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag
     4801 ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa
     4861 atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca
     4921 gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc
     4981 gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc
     5041 acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt
     5101 gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc
     5161 gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag
     5221 caggggatca ttttgcgctt cagccatact tttcatactc ccgccat
//
