LOCUS       sequence-422223-        5261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Riboptoregulator circuit RoRL for blue-light-activated gene 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Ranzani AT, Buchholz K, Blackholm M, Kopkin H, Moglich A
  TITLE     Induction of bacterial expression at the mRNA level by light.
  JOURNAL   Nucleic Acids Res. 2024 Aug 10:gkae678. doi: 10.1093/nar/gkae678.
  PUBMED    39126322
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Aug 10:gkae678. doi: 10.1093/nar/gkae678."
COMMENT     Sequence Label: pRoRL
FEATURES             Location/Qualifiers
     source          1..5261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     200..219
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1440..1469
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1522..1540)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1536..1583
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1637..1654)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             1849..1866
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1876..1893
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     terminator      2073..2159
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2251..2278
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(2479..3270)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3271..3362)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3410..4148)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(4349..5227)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta
       61 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg
      121 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg
      181 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc
      241 tttttaaaaa attcatttgc taaacgcttc aaattctcgt ataattactt ttctccatac
      301 ccgttttttg ggctaacccg ccgaaaccta aggaaataca tagtatgaaa gttaatcgtc
      361 cggcagaacg tgccagcttt ggtagctttg ttctggatgc aggtagcgca cgttttgttg
      421 gtagtgatga actggcactg gttctgggtt ttgcaccggg tgatgttgtt ctgacaccgg
      481 cagttgttct ggcacatctg catcctgatg atcgtctgga atggcaggca ggtctgcagc
      541 gttgtctggc aaccggtcgt ccggtggttg ttaatcatct gctgctgacc gcagaagcag
      601 aaccgcgtcc ggcaatgaca accctgaccg cactgaccga acaggatcgt gtgcgtgcag
      661 ttaccggtgt tattaccgat ctgagcgatc gtgttcgtcg tgcaaccgaa gcagaaattc
      721 gtcaggcagt tcgtgcagca gcagcaaccc gtagcgaaat tgatcaggca aaaggtattg
      781 ttatggcagc ctttgatgtt gatgcagatc aggcatttgc actgctgaaa tggcatagca
      841 gccagagcaa tcgtaaactg cgtgatctgg ccaccggtat gattgaaggt ctggcagcag
      901 ccaatagcgc actgccgctg cgtcgtcgtc tgagcaccgt ttttaccgat atgggttgtc
      961 cggcaccgag caccaaaggt tggaccgttc cggtgaccga tattggtctg cctccgacca
     1021 gcggtctgat tccgacagca ctgctgcctg gtattctgac ccgtgcagca catgatgcaa
     1081 gcgttgcaat taccgttgca gatgttaccg caccggatca gcctctggtt tatgcaaatc
     1141 ctgcatttga acgtctgacc ggttatgcag cagccgaagt tctgggtcgt aattgtcgtt
     1201 ttctgcaggc agaaagcggt gatccgcatg aacgtagcgc aattcgtagc gccattgcaa
     1261 atggtgatgc agtgaccaca ctgattcgta attttcgtca ggatggtcat gccttttgga
     1321 acgaatttca tctgagtccg gttcgtaatg gtgcaggtcg tgttacccat tacattggtt
     1381 atcagctgga tgtgaccgaa cgtgttgaac gtgatcagca gctggaacag ctggcaagcg
     1441 aacagaaact gatttctgaa gaagatctgt aagatccggc tgctaacaaa gcccgaaagg
     1501 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     1561 aacgggtctt gaggggtttt ttgctcggcc gcactcgaga gcttggctgt tttggcggat
     1621 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     1681 agaatttgcc ggtgttgaca taaataccac tggcggtgat actgagcaca tcagcaggcc
     1741 cctctagaaa taattttgtt taactttaaa tagatcacct gctaggttca gcagcgagcc
     1801 ttaagcctca ctgctggaac taggaggaga tataccatgg gcagcagcca tcatcatcat
     1861 catcacagca gcggcctggt gccgcgcggc agccatatgg gatccgaatt cgagctccgt
     1921 cgacaagctt gcggccgcac tcgagcatga gatccctggc ggcagtagcg cggtggtccc
     1981 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
     2041 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     2101 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     2161 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
     2221 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
     2281 cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2341 gacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     2401 acgaccctgc cctgaaccga cgaccgggtc atcgtggccg gatcttgcgg cccctcggct
     2461 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
     2521 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
     2581 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
     2641 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
     2701 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
     2761 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
     2821 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
     2881 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
     2941 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
     3001 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
     3061 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
     3121 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
     3181 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
     3241 cgatacttcg gcgatcaccg cttccctcat actcttcctt tttcaatatt attgaagcat
     3301 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3361 aatagctagc tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac
     3421 acatacaaag ttacccacag attccgtgga taagcagggg actaacatgt gaggcaaaac
     3481 agcagggccg cgccggtggc gtttttccat aggctccgcc ctcctgccag agttcacata
     3541 aacagacgct tttccggtgc atctgtggga gccgtgaggc tcaaccatga atctgacagt
     3601 acgggcgaaa cccgacagga cttaaagatc cccaccgttt ccggcgggtc gctccctctt
     3661 gcgctctcct gttccgaccc tgccgtttac cggatacctg ttccgccttt ctcccttacg
     3721 ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg taggtcgttc
     3781 gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc gccttatccg
     3841 gtaactgttc acttgagtcc aacccggaaa agcacggtaa aacgccactg gcagcagcca
     3901 ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc tcttgaagtg
     3961 tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct gcgaaagcca
     4021 gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca cctccccagg
     4081 tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct caagaagatc
     4141 ctttgatctt ttctactgaa ccgctctaga tttcagtgca atttatctct tcaaatgtag
     4201 cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca gccccgcatg
     4261 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
     4321 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
     4381 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
     4441 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
     4501 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
     4561 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
     4621 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
     4681 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
     4741 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
     4801 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
     4861 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
     4921 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
     4981 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
     5041 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
     5101 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
     5161 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
     5221 cagccatact tttcatactc ccgccattca gagaagaaac c
//
