LOCUS       sequence-422228-        7916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for red-light-induced bacterial gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Meier SSM, Multamaki E, Ranzani AT, Takala H, Moglich A
  TITLE     Leveraging the histidine kinase-phosphatase duality to sculpt 
            two-component signaling.
  JOURNAL   Nat Commun. 2024 Jun 10;15(1):4876. doi: 10.1038/s41467-024-49251-8.
  PUBMED    38858359
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-10"; volume: "15(1):4876. doi"; pages: " 
            10.1038/s41467-024-49251-8"
COMMENT     Sequence Label: pDrDERusk
FEATURES             Location/Qualifiers
     source          1..7916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(82..101)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     292..313
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(542..1357)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(542..1357)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1265..1284
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1349..1368
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1479..2067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1968..1987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2221..2238
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2253..2392
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2318..2337)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2478..2500
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2494..2685)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        complement(6943..7020)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     7226..7245
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     RBS             7574..7596
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7615..7632
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7642..7659
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             7663..7695
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             7743..7760
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7813..7831)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7827..7874
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      join(7911..7916,1..450)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
       61 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      121 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      181 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      241 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      301 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      361 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
      421 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
      481 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt
      541 cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
      601 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
      661 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
      721 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
      781 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
      841 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
      901 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
      961 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     1021 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     1081 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     1141 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     1201 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     1261 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     1321 gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     1381 tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1441 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1501 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1561 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1621 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1681 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1741 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1801 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1861 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1921 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1981 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2041 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2101 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2161 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2221 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     2281 ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct gatgccgcat
     2341 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     2401 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2461 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2521 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc
     2581 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct
     2641 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta
     2701 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat
     2761 acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc
     2821 ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa
     2881 tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat
     2941 aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac
     3001 cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc
     3061 gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct
     3121 caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga taatggcctg
     3181 cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa
     3241 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
     3301 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
     3361 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
     3421 gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatc
     3481 aattgtcaat cgttgagcat gccggcgcgc atcgcgaggc gaaccagctc cgaaaggctg
     3541 ttggcctgca tcttggtcat gacgttggcc cgatacacct cgatggtgcg cgggctgatg
     3601 tcgtactcgc gggcgatcag cttgttggaa aggccggcga tcagcccttc catgacctgg
     3661 cgctccctgg ggctcaacga ggcgacgcgg gcggcgatat cctgcgcgac ggcctcgctc
     3721 ttggcggccg gctcggcctg gcggatcgcc gattcgatca tggcggtgag gcggtcgtcc
     3781 tcgaaaggct tttccagaaa gtcgaccgcc cctaacttca tcgcctcgac cgcgagcggc
     3841 acgtcgccgt gaccggtcat gatgaggatc ggaaaggggc tttgctgcgc cttcatccgc
     3901 ttcaacagct cgatgccgtc aaggcccggc atgcgcacgt cggagacgac gcagccgaag
     3961 gagagacccg gcagggcgtc gagaaaggct tgcgcgtcgt caaacagcgt gacgccgaag
     4021 ccggcagaat ccagcaggaa attcagcgaa tcccgcatcg ccgcgtcgtc gtcgatgacg
     4081 tagatatgtc ccttggtcgt catgctagac ctcctatcat ctcgtcggct gccgggaggg
     4141 tgaagcggaa ggtcgccccg cccgatgcgt tgctctcggc ccacatgcgc ccgccgtgag
     4201 cttcgatgat cgagcggctg atggacagtc ccacgcccat gccggtgtcc ttggtggtga
     4261 agaaagtctg aaacaggttc ggaatgacgt cgtcctggaa accgctgccg gtgtcggaca
     4321 cttcgacctc gatcatgtcg tcggcggcgg gggtgttggt gacgacgagc tcgcgtcgct
     4381 gcgactgagc catcgcttcc agcgcgttgc ggaacaggtt gaccaggacc tgctggatct
     4441 gcacccggtc ggcgagaacg agatcggcgc ccggatcgag actgaagcgg agctgcacgt
     4501 tctgctcgcg cgcgccggca agcccgagcg cgccggcctc ctcgatcagc ttggagagac
     4561 tctcgacccg cttctccgat tcgccgcggg caacgaagtc gcgcaggcgc cggatgatct
     4621 ggccggcgcg cagcgcctgc tcggcggcgc ggtccagggc gctttcgacc ttcggtgtgt
     4681 tcggatcact gctgccggca agcagccgcc gcgagccctt catgtagttg ctgatcgccg
     4741 ccagcggctg gttgagctcg tgcgcgagcg cggacgccat ttcgcccatg gcgctcagcc
     4801 tggagacgtg gacgagctcg gagcgctcgc ccagcgcccc ggtcaatgtg tcacgtagat
     4861 cctgcgcctc ctcgatttcg ccgggatgcc agggctcggc gtagccgcgt ttttcttcga
     4921 ggtaggtgtc gaatgagtgg cgcggcccga ggtcgtcttt cgcctggtca ggagtggccc
     4981 cgccccaggc gacctccagc cgcagttcgg gccgcagcca gacgaggcac tccgaccagc
     5041 cctcgcccac gctgatggcg agcaggcccg ctgcgctggg ggcgagatcg gcgccggcgg
     5101 gccacagttg gcctagcgcg tcggtctgga ccagggcgcc cggctgggtt tcgagccacg
     5161 ccagcagcgc gtccaccgcc ggggcaggcg gcacttcacc caacgtctgc cagcggccct
     5221 cgaaacgcag aatcaggccc ccggcccgca tcaggcccag caggtcaagc gccgggtcac
     5281 tgagggtgtc gtgcggcgag agcgagtgcg ccgccgcgag ggccacccgc gcgtggtgct
     5341 cccgcaggct ctggcgaaag gccgccacgt ccgccgcttc cttgacctga acttgcaggc
     5401 tcagcaagcg gcccaggtat tcgagcgtgg ttcgcaggtc gggcggcaac acgtagggcg
     5461 tctggtggtg gcaggcgatc aggccccaga gctggccgcc gaccaccacc gacaccgaca
     5521 ggctcgaccc gacgcccatg ttccgcaggt actgcatgtg catgggcgag gtggcgcgca
     5581 gcacggcgcc gcccagcggg gtgggcgcat tcgtctgcgg gttgaggacg ggatcgagcg
     5641 gcacggcggc ggcgcgggtg tcggcggtca ggcgcagcag gtgccgggtg tagagcgcgc
     5701 gggcctgcgc cggaatgtcc gacgcgggaa aacggtggcc cagaaaggcg tgcagcccct
     5761 cacggcgggc ctcggcaatc acttcgccgg tggcgtcggg ggcaaatttg tagagcatca
     5821 cccggtcaaa gcccgtcagc tcgcggaccg tctgggtcgc cacctcgccc agcgcccgca
     5881 ggttgggggc actttcgagc gcgaacatcg cgttgcgcag cgcgtgcggc ccggtgctgt
     5941 cccaggcctc cgtcggctcg aattccagaa tcagcaactc gccgacccgg tgcaccgtca
     6001 gcgaaaggtg cccggcggca ggccagtcca gcgttgcgcg gtattgcagg gcgtcggggc
     6061 agccgggggg cagggccgct tgcagcgcgg gccactgctc gggcagcagt gcggcgaggg
     6121 tctgtccgcg cagcactgtg ggttcctgtc ccagaaaagt ggccgcgttg aggctcatct
     6181 ggagcacctc gccgctgtgc ccgtcggcag tgagcagggc gccgtgcggc tggatgctgc
     6241 cgggaatatg aatcggctcg cgctcgcagt tctcggtggt aatttccggg ccaccaaggt
     6301 aaagcggtgg aaaaaagggc aacgggtccc ggctcatgcg tgggcgacct caggcacttg
     6361 gaccgcttca gcttccgaca gcccctgcaa ggcgcggcgg aaggccccga aggtctgccg
     6421 cgctcccgcc acgacctctg ccgcgtcttc tgccggcacg ctggcttcca gcacttcccc
     6481 gaacgagcgc cagcgcgggc cggtctgggg gccatagccg gagaaatagg cgctgccctg
     6541 ctcagggctt agccccagcg tgcgcctgag atggcggctg atgagctgtc cgcctagcgt
     6601 cgcgccttca agcacgtaaa gcgctccgaa ggcctgcgcc agagtggtca gctcgggcac
     6661 agacagtggc gcctgctgat ttgacggccc ttcccccatt acggcaaggt ccagagcgag
     6721 cagatgggcc ttgcggcgct cgggccacgc cagctcgtcc ggcagcggca gggcgtcgag
     6781 ttgtgcttcc agcggcgcga ccaccgcttg caggcgccgc agcacctcca gatagccccg
     6841 ccgggtcagc gtctcactca tcacgggcat cagggcctcg acctcgcggt gcaggtccag
     6901 ggtctccctt tgtaaaagct tcatgatcat gagtcggagc acattcacca ccctcaattg
     6961 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc
     7021 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     7081 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     7141 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     7201 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     7261 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctacgccc
     7321 gtgatcctga tcaccggcta tccggacgaa aacatctcga cccgggccgc cgaggccggc
     7381 gtaaaagacg tggttttgaa gccgcttctc gacgaaaacc tgctcaagcg tatccgccgc
     7441 gccatccagg accggcctcg ggcatgacct acggggttct acgtaaggca ccccccttaa
     7501 gatatcgctc gaaattttcg aacctcccga taccgcgtac caatgcgtca tcacaacgga
     7561 gtctagaaat aattttgttt aactttaaga aggagatata ccatgggcag cagccatcat
     7621 catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatggctag catgactggt
     7681 ggacagcaaa tgggtcggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg
     7741 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
     7801 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     7861 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgc
//
