LOCUS       sequence-422230-        8054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for red-light-induced bacterial gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Meier SSM, Multamaki E, Ranzani AT, Takala H, Moglich A
  TITLE     Leveraging the histidine kinase-phosphatase duality to sculpt 
            two-component signaling.
  JOURNAL   Nat Commun. 2024 Jun 10;15(1):4876. doi: 10.1038/s41467-024-49251-8.
  PUBMED    38858359
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-10"; volume: "15(1):4876. doi"; pages: " 
            10.1038/s41467-024-49251-8"
COMMENT     Sequence Label: pDmDERusk
FEATURES             Location/Qualifiers
     source          1..8054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(51..98)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     94..112
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(165..182)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(230..262)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(266..283)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(293..310)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             329..351
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(680..699)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        905..982
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5378..5569
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5563..5585)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5671..5810
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5726..5745
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5825..5842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5996..6584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6076..6095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6695..6714)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6706..7521
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6706..7521
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6779..6798)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(join(7613..8054,1..14))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7750..7771)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7962..7981
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctacagggc gcgtcccatt cgccaatccg gatatagttc ctcctttcag caaaaaaccc
       61 ctcaagaccc gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca
      121 gccaactcag cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg
      181 tgctcgagtg cggccgcaag cttgtcgacg gagctcgaat tcggatccga cccatttgct
      241 gtccaccagt catgctagcc atatggctgc cgcgcggcac caggccgctg ctgtgatgat
      301 gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa aattatttct
      361 agactccgtt gtgatgacgc attggtacgc ggtatcggga ggttcgaaaa tttcgagcga
      421 tatcttaagg ggggtgcctt acgtagaacc ccgtaggtca tgcccgaggc cggtcctgga
      481 tggcgcggcg gatacgcttg agcaggtttt cgtcgagaag cggcttcaaa accacgtctt
      541 ttacgccggc ctcggcggcc cgggtcgaga tgttttcgtc cggatagccg gtgatcagga
      601 tcacgggcgt agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
      661 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
      721 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
      781 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
      841 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat
      901 cccggacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
      961 gagtcaattg agggtggtga atgtgctccg actcatgatc atgaagcttt tacaaaggga
     1021 gaccctggac ctgcaccgcg aggtcgaggc cctgatgccc gtgatgagtg agacgctgac
     1081 ccggcggggc tatctggagg tgctgcggcg cctgcaagcg gtggtcgcgc cgctggaagc
     1141 acaactcgac gccctgccgc tgccggacga gctggcgtgg cccgagcgcc gcaaggccca
     1201 tctgctcgct ctggaccttg ccgtaatggg ggaagggccg tcaaatcagc aggcgccact
     1261 gtctgtgccc gagctgacca ctctggcgca ggccttcgga gcgctttacg tgcttgaagg
     1321 cgcgacgcta ggcggacagc tcatcagccg ccatctcagg cgcacgctgg ggctaagccc
     1381 tgagcagggc agcgcctatt tctccggcta tggcccccag accggcccgc gctggcgctc
     1441 gttcggggaa gtgctggaag ccagcgtgcc ggcagaagac gcggcagagg tcgtggcggg
     1501 agcgcggcag accttcgggg ccttccgccg cgccttgcag gggctgtcgg aagctgaagc
     1561 ggtccaagtg cctgaggtcg cccacgcatg acagaacgtc cggcagcgcg tccgggtgca
     1621 gcacgtatga ccggtggtga tgattttctg acaccggcac atttaggtgg tccgcctgtt
     1681 gataccgata attgtgcacg tgaaccgatt catattccgg gtgccgttca gccgcatggt
     1741 gcactgctgg ttctgcgtgc agatgatgaa cgtgcagttc aggttagcgc aaatagcgaa
     1801 gcatttgttg gtgttgcacc tgatgcgctg ctgggtcagc atctggccga tctggttggt
     1861 gatcagggtg cagccgcact gcgtgccgca tttgcacagg caggtccgag cgcaatgacc
     1921 ctgacctttc gtaatggtcg tacctatgac gttaccgcac gtcgtgatgg cgaactgctg
     1981 ctgatggaac tggaaccgcc tgatgttcgt gcaggcactc cggcactgta tcatgcaatt
     2041 cgtgatgcac tgggtgcact ggaacatgca ccggatctgc atgccctgct ggatgttgca
     2101 gcacagcgtg ttcgtgccct gaccggtctg gatcgtgtga tgatctatcg ttttgcagca
     2161 gatgatagcg gtgaagttgt tgccgaagca cgtgcaccgc atttacatgc atttctgggt
     2221 catcgttttc cggaatcaga tattccgcgt caggcacgtg cgctgtatgt gcagcattta
     2281 ctgcgtttta ccgcagatgc cggtggtggt caggttccgc tggttcctgc actgaatccg
     2341 gttaccaatg caccgctgca gatgggtgcc ctggttttac gtgcaaccag tccggttcat
     2401 ctgcagtatc tgcgtaatat gggtgttatt gcaagcatga gcgttagcat tgttcaggat
     2461 ggtcgtctgt ggggtctgat tgcatgtcat catggtgccg cacatgttgt tccgcaggca
     2521 acccgtgatg catgtgaatt tctgggacgt gttctgagcc tgcagattac cgcaaaacgt
     2581 gatgcagcag ttaatgcccg tcgtgcagcc ctgggtgccc agcatgcacg tctggttacc
     2641 gcagttaccg gtacgctgac accgctggat gcactgaccc gtgccgatct gaatctgcct
     2701 ggttttctgg atacagccgg tgccgcagtt cgtatggatg gtggtacacg taccctgggt
     2761 gttaccccga gtgatgagga tctggaacat ctggttgcat ggctgcgtgc acagggtaca
     2821 ccgagcgttt gtaccgatgc gctggcacgt acctatccgc caggtgcact gtttatggaa
     2881 cgtgcaagcg gtgttctggg tgtgagcatt agcggtaatt gggatgaata tctgctgtgg
     2941 tttcgtccgg aaattccggc aaccattacc tggggtggtg atccgcataa agccgttcag
     3001 gtttcagatg atggtacagc acgtctgact ccgcgtgcca gctttagcag ctatgttgaa
     3061 gcagttcgtg gtgttgccca gccgtggcag gcaggcgatc tggatgcagc actggatctg
     3121 cgtggtgcat tactggaagt tcagggtgcc cgtctggccg cgttgcagga cctcaatgca
     3181 cgcttagagc gtgctaatcg tgagcatcag cagacacaag cacgtctcca ggaactgcaa
     3241 tccgagctcg tccacgtctc caggctgagc gccatgggcg aaatggcgtc cgcgctcgcg
     3301 cacgagctca accagccgct ggcggcgatc agcaactaca tgaagggctc gcggcggctg
     3361 cttgccggca gcagtgatcc gaacacaccg aaggtcgaaa gcgccctgga ccgcgccgcc
     3421 gagcaggcgc tgcgcgccgg ccagatcatc cggcgcctgc gcgacttcgt tgcccgcggc
     3481 gaatcggaga agcgggtcga gagtctctcc aagctgatcg aggaggccgg cgcgctcggg
     3541 cttgccggcg cgcgcgagca gaacgtgcag ctccgcttca gtctcgatcc gggcgccgat
     3601 ctcgttctcg ccgaccgggt gcagatccag caggtcctgg tcaacctgtt ccgcaacgcg
     3661 ctggaagcga tggctcagtc gcagcgacgc gagctcgtcg tcaccaacac ccccgccgcc
     3721 gacgacatga tcgaggtcga agtgtccgac accggcagcg gtttccagga cgacgtcatt
     3781 ccgaacctgt ttcagacttt cttcaccacc aaggacaccg gcatgggcgt gggactgtcc
     3841 atcagccgct cgatcatcga agctcacggc gggcgcatgt gggccgagag caacgcatcg
     3901 ggcggggcga ccttccgctt caccctcccg gcagccgacg agatgatagg aggtctagca
     3961 tgacgaccaa gggacatatc tacgtcatcg acgacgacgc ggcgatgcgg gattcgctga
     4021 atttcctgct ggattctgcc ggcttcggcg tcacgctgtt tgacgacgcg caagcctttc
     4081 tcgacgccct gccgggtctc tccttcggct gcgtcgtctc cgacgtgcgc atgccgggcc
     4141 ttgacggcat cgagctgttg aagcggatga aggcgcagca aagccccttt ccgatcctca
     4201 tcatgaccgg tcacggcgac gtgccgctcg cggtcgaggc gatgaagtta ggggcggtcg
     4261 actttctgga aaagcctttc gaggacgacc gcctcaccgc catgatcgaa tcggcgatcc
     4321 gccaggccga gccggccgcc aagagcgagg ccgtcgcgca ggatatcgcc gcccgcgtcg
     4381 cctcgttgag ccccagggag cgccaggtca tggaagggct gatcgccggc ctttccaaca
     4441 agctgatcgc ccgcgagtac gacatcagcc cgcgcaccat cgaggtgtat cgggccaacg
     4501 tcatgaccaa gatgcaggcc aacagccttt cggagctggt tcgcctcgcg atgcgcgccg
     4561 gcatgctcaa cgattgacaa ttgatgtaag ttagctcact cattaggcac cgggatctcg
     4621 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     4681 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     4741 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     4801 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     4861 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg
     4921 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
     4981 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
     5041 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
     5101 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc
     5161 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct
     5221 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc
     5281 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac
     5341 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc
     5401 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
     5461 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
     5521 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     5581 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     5641 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     5701 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     5761 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     5821 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6421 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6661 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     6721 cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg
     6781 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
     6841 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     6901 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     6961 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca
     7021 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     7081 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     7141 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     7201 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     7261 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     7321 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     7381 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     7441 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     7501 atgctcgatg agtttttcta agaattaatt catgagcgga tacatatttg aatgtattta
     7561 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     7621 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     7681 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     7741 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     7801 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     7861 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     7921 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     7981 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     8041 gcgcttaatg cgcc
//
