LOCUS       sequence-422239-        9600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express split-KRas fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C
  TITLE     Standardized Parts for Activation of Small GTPase Signaling in 
            Living Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 
            10.1002/anie.202403499.
  PUBMED    39058298
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499."
COMMENT     Sequence Label: 
            pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..9600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..599
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     550..570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     596..620
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          760..892
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     875..894
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        937..955
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(961..963,964..966,967..1677)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(2883..3457,3458..3460,3461..3469)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3050..3067)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3277..3296
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4081..4098
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(4369..4371,4372..5082)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    5174..5295
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5185..5204)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5239..5258
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5481..5936
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5568..5587)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5778..5799
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6053..6410
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6053..6244
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6076..6096)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6261..6396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6461..7255
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6515..6534)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7125..7144
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7319..7367
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7408..7427)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7527..7549
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7587..7605)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7673..7777
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7778..7846,7847..8638)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7996..8015)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8809..9397
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9298..9317
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccatgttggc attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg cgatcgcccg
      541 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      601 gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg
      661 ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt gactctctta
      721 aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga
      781 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc
      841 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac
      901 tcccagttca attacagctc ttaaggctag agtacttaat acgactcact ataggctagc
      961 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1021 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1081 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1141 ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     1201 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1261 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1321 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1381 aagctggagt acaacgccat cagcgacaac gtctatatca ccgccgacaa gcagaagaac
     1441 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1501 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1561 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1621 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc
     1681 ggtggctcat ctggcggagg tatggctgcg agcgatgaag ttaatcttat tgagagcaga
     1741 acagtggttc ctctcaatac atgggtttta atatccaact tcaaagtagc ctacaatatc
     1801 cttcgtcgcc ctgatggaac ctttaaccga cacttagctg agtatctaga ccgtaaagtc
     1861 actgcaaacg ccaatccggt tgatggggtt ttctcgttcg atgtcttgat tgatcgcagg
     1921 atcaatcttc taagcagagt ctatagacca gcttatgcag atcaagagca acctcctagt
     1981 attttagatc tcgagaagcc tgttgatggc gacattgtcc ctgttatatt gttcttccat
     2041 ggaggtagct ttgctcattc ttctgcaaac agtgccatct acgatactct ttgtcgcagg
     2101 cttgttggtt tgtgcaagtg tgttgttgtc tctgtgaatt atcggcgtgc accagagaat
     2161 ccataccctt gtgcttatga tgatggttgg attgctctta attgggttaa ctcgagatct
     2221 tggcttaaat ccaagaaaga ctcaaaggtc catattttct tggctggtga tagctctgga
     2281 ggtaacatcg cgcataatgt ggctttaaga gcgggtgaat cgggaatcga tgttttgggg
     2341 aacattctgc tgaatcctat gtttggtggg aatgagagaa cggagtctga gaaaagtttg
     2401 gatgggaaat actttgtgac ggttagagac cgcgattggt actggaaagc gtttttaccc
     2461 gagggagaag atagagagca tccagcgtgt aatccgttta gcccgagagg gaaaagctta
     2521 gaaggagtga gtttccccaa gagtcttgtg gttgtcgcgg gtttggattt gattagagat
     2581 tggcagttgg catacgcgga agggctcaag aaagcgggtc aagaggttaa gcttatgcat
     2641 ttagagaaag caactgttgg gttttacctc ttgcctaata acaatcattt ccataatgtt
     2701 atggatgaga tttcggcgtt tgtaaacgcg gaatgtcaga tctcgtacgc gtcccggggc
     2761 ggtggctcat ctggcggagg tgagctcatg actgaatata aacttgtggt agttggagct
     2821 ggttaagaat tcacgcgtcg agcatgcatc tagggcggcc aattccgccc ctctcccccc
     2881 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     2941 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     3001 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     3061 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     3121 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     3181 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     3241 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     3301 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     3361 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     3421 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc gggatcctct
     3481 agagtcgaca tgggcgtagg caagaatgcc ttgacgatac agctaattca gaatcatttt
     3541 gtggacgaat atgatccaac aatagaggat tcctacagga agcaagtagt aattgatgga
     3601 gaaacctgtc tcttggatat tctcgacaca gcaggtcaag aggagtacag tgcaatgagg
     3661 gaccagtaca tgaggactgg ggagggcttt ctttgtgtat ttgccataaa taatactaaa
     3721 tcatttgaag atattcacca ttatagagaa caaattaaaa gagttaagga ctctgaagat
     3781 gtacctatgg tcctagtagg aaataaatgt gatttgcctt ccagaacagt agacacaaaa
     3841 caggctcagg acttagcaag aagttatgga attcctttta ttgaaacatc agcaaagaca
     3901 agacagggtg ttgatgatgc cttctataca ttagttcgag aaattcgaaa acataaagaa
     3961 aagatgagca aagatggtgg ggatcccaat tgggagctcg tgtacacggc gcgcctgcag
     4021 ggaggtggct catctggcgg aggtcagatc tcgtacgcgt cccggggcat gaagagagat
     4081 catcatcatc atcatcatca agataagaag actatgatga tgaatgaaga agacgacggt
     4141 aacggcatgg atgagcttct agctgttctt ggttacaagg ttaggtcatc cgaaatggct
     4201 gatgttgctc agaaactcga gcagcttgaa gttatgatgt ctaatgttca agaagacgat
     4261 ctttctcaac tcgctactga gactgttcac tataatccgg cggagcttta cacgtggctt
     4321 gattctatgc tcaccgacct taatggcggt ggctcatctg gcggaggtgt gagcaagggc
     4381 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4441 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4501 aagctcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctc
     4561 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     4621 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4681 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4741 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4801 tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     4861 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     4921 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     4981 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5041 accgccgccg ggatcactct cggcatggac gagctgtaca agaaaaaaaa gaagaaaaag
     5101 agcaagacca aatgcgtgat tatgtaagcg gccgcttccc tttagtgagg gttaatgctt
     5161 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     5221 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     5281 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     5341 atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat ccgataagga
     5401 tcgatccggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     5461 gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5521 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5581 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5641 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5701 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5761 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5821 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5881 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg
     5941 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg cggatctgcg
     6001 cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg
     6061 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6241 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6301 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6361 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgatt
     6421 cttctgacac aacagtctcg aacttaaggc tagagccacc atgattgaac aagatggatt
     6481 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     6541 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     6601 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     6661 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     6721 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     6781 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     6841 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     6901 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     6961 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     7021 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     7081 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     7141 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     7201 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     7261 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa
     7321 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat
     7381 aaggatccgc gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7441 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7501 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7561 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7621 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7681 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7741 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7801 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7861 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7921 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7981 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8041 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8101 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8161 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8221 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8281 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8341 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8401 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8461 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8521 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8581 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8641 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8701 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8761 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8821 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8881 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8941 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9001 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9061 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9121 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9181 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9241 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9301 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9361 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9421 acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct tcaatattgg
     9481 ccattagcca tattattcat tggttatata gcataaatca atattggcta ttggccattg
     9541 catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg
//
