LOCUS       sequence-422240-        9600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express split-KRas fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C
  TITLE     Standardized Parts for Activation of Small GTPase Signaling in 
            Living Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 
            10.1002/anie.202403499.
  PUBMED    39058298
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499."
COMMENT     Sequence Label: 
            pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..9600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..36
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        290..647
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        290..481
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(313..333)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      498..633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             698..1492
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(752..771)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1362..1381
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1556..1604
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(1645..1664)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1764..1786
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1824..1842)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1910..2014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2015..2083,2084..2875)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2233..2252)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3046..3634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3535..3554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3845..4224
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4225..4436
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4387..4407
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4433..4457
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4597..4729
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4712..4731
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4774..4792
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4798..4800,4801..4803,4804..5514)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(6720..7294,7295..7297,7298..7306)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6887..6904)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7114..7133
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7918..7935
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(8206..8208,8209..8919)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    9011..9132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9022..9041)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9076..9095
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      join(9318..9600,1..173)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9405..9424)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
       61 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      121 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg
      181 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgcgg atctgcgcag
      241 caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa
      301 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
      361 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      421 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      481 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      541 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      601 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttgattctt
      661 ctgacacaac agtctcgaac ttaaggctag agccaccatg attgaacaag atggattgca
      721 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      781 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      841 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      901 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      961 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     1021 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     1081 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     1141 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     1201 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1261 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1321 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1381 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1441 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1501 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgatg gccgcaataa
     1561 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagcgataag
     1621 gatccgcgta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     1681 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     1741 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     1801 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1861 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1921 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1981 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2041 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2101 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2161 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2221 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2281 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2341 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2401 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2461 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2521 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2581 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2641 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2701 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2761 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2821 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2881 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2941 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3001 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3061 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3181 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3661 gttcctggcc ttttgctggc cttttgctca catggctcga cagatcttca atattggcca
     3721 ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     3781 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     3841 tgttggcatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     3901 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3961 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4021 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4081 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     4141 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     4201 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
     4261 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     4321 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactgcga tcgcccgccc
     4381 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     4441 tagtgaaccg tcagatcact agaagcttta ttgcggtagt ttatcacagt taaattgcta
     4501 acgcagtcag tgcttctgac acaacagtct cgaacttaag ctgcagtgac tctcttaagg
     4561 tagccttgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag
     4621 gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga
     4681 taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc
     4741 cagttcaatt acagctctta aggctagagt acttaatacg actcactata ggctagcatg
     4801 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4861 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4921 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4981 gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     5041 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     5101 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     5161 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5221 ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
     5281 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     5341 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     5401 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     5461 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt
     5521 ggctcatctg gcggaggtat ggctgcgagc gatgaagtta atcttattga gagcagaaca
     5581 gtggttcctc tcaatacatg ggttttaata tccaacttca aagtagccta caatatcctt
     5641 cgtcgccctg atggaacctt taaccgacac ttagctgagt atctagaccg taaagtcact
     5701 gcaaacgcca atccggttga tggggttttc tcgttcgatg tcttgattga tcgcaggatc
     5761 aatcttctaa gcagagtcta tagaccagct tatgcagatc aagagcaacc tcctagtatt
     5821 ttagatctcg agaagcctgt tgatggcgac attgtccctg ttatattgtt cttccatgga
     5881 ggtagctttg ctcattcttc tgcaaacagt gccatctacg atactctttg tcgcaggctt
     5941 gttggtttgt gcaagtgtgt tgttgtctct gtgaattatc ggcgtgcacc agagaatcca
     6001 tacccttgtg cttatgatga tggttggatt gctcttaatt gggttaactc gagatcttgg
     6061 cttaaatcca agaaagactc aaaggtccat attttcttgg ctggtgatag ctctggaggt
     6121 aacatcgcgc ataatgtggc tttaagagcg ggtgaatcgg gaatcgatgt tttggggaac
     6181 attctgctga atcctatgtt tggtgggaat gagagaacgg agtctgagaa aagtttggat
     6241 gggaaatact ttgtgacggt tagagaccgc gattggtact ggaaagcgtt tttacccgag
     6301 ggagaagata gagagcatcc agcgtgtaat ccgtttagcc cgagagggaa aagcttagaa
     6361 ggagtgagtt tccccaagag tcttgtggtt gtcgcgggtt tggatttgat tagagattgg
     6421 cagttggcat acgcggaagg gctcaagaaa gcgggtcaag aggttaagct tatgcattta
     6481 gagaaagcaa ctgttgggtt ttacctcttg cctaataaca atcatttcca taatgttatg
     6541 gatgagattt cggcgtttgt aaacgcggaa tgtcagatct cgtacgcgtc ccggggcggt
     6601 ggctcatctg gcggaggtga gctcatgact gaatataaac ttgtggtagt tggagctggt
     6661 taagaattca cgcgtcgagc atgcatctag ggcggccaat tccgcccctc tccccccccc
     6721 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     6781 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     6841 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     6901 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     6961 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     7021 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     7081 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     7141 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     7201 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     7261 gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccggg atcctctaga
     7321 gtcgacatgg gcgtaggcaa gagtgccttg acgatacagc taattcagaa tcattttgtg
     7381 gacgaatatg atccaacaat agaggattcc tacaggaagc aagtagtaat tgatggagaa
     7441 acctgtctct tggatattct cgacacagca ggtctggagg agtacagtgc aatgagggac
     7501 cagtacatga ggactgggga gggctttctt tgtgtatttg ccataaataa tactaaatca
     7561 tttgaagata ttcaccatta tagagaacaa attaaaagag ttaaggactc tgaagatgta
     7621 cctatggtcc tagtaggaaa taaatgtgat ttgccttcca gaacagtaga cacaaaacag
     7681 gctcaggact tagcaagaag ttatggaatt ccttttattg aaacatcagc aaagacaaga
     7741 cagggtgttg atgatgcctt ctatacatta gttcgagaaa ttcgaaaaca taaagaaaag
     7801 atgagcaaag atggtgggga tcccaattgg gagctcgtgt acacggcgcg cctgcaggga
     7861 ggtggctcat ctggcggagg tcagatctcg tacgcgtccc ggggcatgaa gagagatcat
     7921 catcatcatc atcatcaaga taagaagact atgatgatga atgaagaaga cgacggtaac
     7981 ggcatggatg agcttctagc tgttcttggt tacaaggtta ggtcatccga aatggctgat
     8041 gttgctcaga aactcgagca gcttgaagtt atgatgtcta atgttcaaga agacgatctt
     8101 tctcaactcg ctactgagac tgttcactat aatccggcgg agctttacac gtggcttgat
     8161 tctatgctca ccgaccttaa tggcggtggc tcatctggcg gaggtgtgag caagggcgag
     8221 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     8281 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     8341 ctcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctcggc
     8401 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     8461 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     8521 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     8581 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     8641 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     8701 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     8761 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     8821 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     8881 gccgccggga tcactctcgg catggacgag ctgtacaaga aaaaaaagaa gaaaaagagc
     8941 aagaccaaat gcgtgattat gtaagcggcc gcttcccttt agtgagggtt aatgcttcga
     9001 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     9061 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     9121 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     9181 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatccg ataaggatcg
     9241 atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     9301 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     9361 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     9421 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9481 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     9541 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
//
