LOCUS       sequence-422242-        8874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express split-KRas fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C
  TITLE     Standardized Parts for Activation of Small GTPase Signaling in 
            Living Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 
            10.1002/anie.202403499.
  PUBMED    39058298
REFERENCE   2  (bases 1 to 8874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499."
COMMENT     Sequence Label: 
            pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..8874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      135..270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             335..1129
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(389..408)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     999..1018
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1193..1241
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(1282..1301)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1401..1423
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1461..1479)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1547..1651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1652..1720,1721..2512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1870..1889)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2683..3271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3172..3191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3482..3861
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3862..4073
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4024..4044
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4070..4094
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4234..4366
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4349..4368
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4411..4429
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4435..4437,4438..4440,4441..5151)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5176..5445
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     misc_feature    join(5592..6166,6167..6169,6170..6178)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5759..5776)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5986..6005
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6772..7092
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(7117..7119,7120..7830)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    7922..8043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7933..7952)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7987..8006
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      8229..8684
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8316..8335)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8526..8547
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(8801..8874,1..284)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(8801..8874,1..118)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8824..8844)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
       61 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      121 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
      241 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt gattcttctg
      301 acacaacagt ctcgaactta aggctagagc caccatgatt gaacaagatg gattgcacgc
      361 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
      421 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
      481 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
      541 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
      601 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
      661 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
      721 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
      781 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
      841 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
      901 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
      961 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     1021 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     1081 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     1141 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgatggcc gcaataaaat
     1201 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat
     1261 ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     1321 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     1381 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     1441 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1501 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1561 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1621 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1681 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1741 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1801 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1861 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1921 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1981 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2041 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2101 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2161 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     2221 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     2281 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2341 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     2401 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2461 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2521 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2581 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2641 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2701 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2761 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2821 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2881 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2941 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3001 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3061 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3121 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3181 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3241 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3301 cctggccttt tgctggcctt ttgctcacat ggctcgacag atcttcaata ttggccatta
     3361 gccatattat tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg
     3421 ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg accgccatgt
     3481 tggcattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     3541 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     3601 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     3661 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     3721 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
     3781 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
     3841 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
     3901 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     3961 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actgcgatcg cccgccccgt
     4021 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     4081 tgaaccgtca gatcactaga agctttattg cggtagttta tcacagttaa attgctaacg
     4141 cagtcagtgc ttctgacaca acagtctcga acttaagctg cagtgactct cttaaggtag
     4201 ccttgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac aagacaggtt
     4261 taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     4321 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt ccactcccag
     4381 ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc tagcatggtg
     4441 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4501 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4561 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4621 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     4681 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4741 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4801 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4861 gagtacaacg ccatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     4921 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     4981 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5041 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5101 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtggc
     5161 tcatctggcg gaggtgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt
     5221 ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa
     5281 cggggccccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg
     5341 gaggcccaag agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcacccaa
     5401 gcctgggacc tctattatca tgtgttccga cgaatctcaa agcagcagat ctcgtacgcg
     5461 tcccggggcg gtggctcatc tggcggaggt gagctcatga ctgaatataa acttgtggta
     5521 gttggagctg gttaagaatt cacgcgtcga gcatgcatct agggcggcca attccgcccc
     5581 tctccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     5641 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5701 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5761 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5821 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     5881 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     5941 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     6001 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     6061 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     6121 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg
     6181 ggatcctcta gaatgggcgt aggcaagagt gccttgacga tacagctaat tcagaatcat
     6241 tttgtggacg aatatgatcc aacaatagag gattcctaca ggaagcaagt agtaattgat
     6301 ggagaaacct gtctcttgga tattctcgac acagcaggtc tggaggagta cagtgcaatg
     6361 agggaccagt acatgaggac tggggagggc tttctttgtg tatttgccat aaataatact
     6421 aaatcatttg aagatattca ccattataga gaacaaatta aaagagttaa ggactctgaa
     6481 gatgtaccta tggtcctagt aggaaataaa tgtgatttgc cttccagaac agtagacaca
     6541 aaacaggctc aggacttagc aagaagttat ggaattcctt ttattgaaac atcagcaaag
     6601 acaagacagg gtgttgatga tgccttctat acattagttc gagaaattcg aaaacataaa
     6661 gaaaagatga gcaaagatgg tggggatccc aattgggagc tcgtgtacac ggcgcgcctg
     6721 cagggaggtg gctcatctgg cggaggtcag atctcgtacg cgtcccgggg cggagtgcag
     6781 gtggaaacca tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg
     6841 gtgcactaca ccgggatgct tgaagatgga aagaaatttg attcctcccg ggacagaaac
     6901 aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt
     6961 gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt
     7021 gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt
     7081 ctaaaactgg aaggcggtgg ctcatctggc ggaggtgtga gcaagggcga ggagctgttc
     7141 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     7201 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctcatctgc
     7261 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctcgg ctacggcctg
     7321 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     7381 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     7441 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     7501 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     7561 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     7621 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     7681 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
     7741 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     7801 atcactctcg gcatggacga gctgtacaag aaaaaaaaga agaaaaagag caagaccaaa
     7861 tgcgtgatta tgtaagcggc cgcttccctt tagtgagggt taatgcttcg agcagacatg
     7921 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     7981 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     8041 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     8101 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcc gataaggatc gatccgggct
     8161 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     8221 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     8281 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     8341 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8401 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     8461 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8521 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     8581 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     8641 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     8701 ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca gcaccatggc
     8761 ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc
     8821 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggca
//
