LOCUS       sequence-422245-        8892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express split-RhoA fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C
  TITLE     Standardized Parts for Activation of Small GTPase Signaling in 
            Living Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 
            10.1002/anie.202403499.
  PUBMED    39058298
REFERENCE   2  (bases 1 to 8892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499."
COMMENT     Sequence Label: 
            pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..8892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..42
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(182..637)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(319..340)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     531..550
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    823..944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(860..879)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     914..933
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(1036..1746,1747..1749))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(1774..2094)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    join(2700..2708,2709..2711,2712..3286)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2873..2892)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     3102..3119
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(3439..3708)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             complement(join(3733..4443,4444..4446,4447..4449))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(4455..4473)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4516..4535)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          4518..4650
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(4790..4814)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4811..5022)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4840..4860)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5023..5402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5613..6201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5693..5712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6372..7163,7164..7232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6995..7014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7233..7337)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7405..7423
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7461..7483)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7583..7602
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    7643..7691
                     /note="synthetic polyadenylation signal"
     CDS             complement(7755..8549)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7866..7885)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8476..8495
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(8600..8892,1..65))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8614..8749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        join(8766..8892,1..65)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
ORIGIN
        1 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc
       61 ctcagaaggt acctaaccaa gttcctcttt cagaggttat ttcaggccat ggtgctgcgc
      121 agatccgcgt atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
      181 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
      241 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
      301 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
      361 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
      421 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
      481 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
      541 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      601 acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca
      661 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc ggatcgatcc
      721 ttatcggatt ttaccacatt tgtagaggtt ttacttgctt taaaaaacct cccacatctc
      781 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      841 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      901 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgctcgaag
      961 cattaaccct cactaaaggg aagcggccgc ttacataatc acgcatttgg tcttgctctt
     1021 tttcttcttt tttttcttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
     1081 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gcttggactg
     1141 gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
     1201 ctggtagtgg tcggcgagct gcacgccgcc gtcctcgatg ttgtggcgga tcttgaagtt
     1261 ggccttgatg ccgttcttct gcttgtcggc ggtgatatag acgttgtggc tgttgtagtt
     1321 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
     1381 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
     1441 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
     1501 gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cactgcaggc cgtagccgag
     1561 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgagcttcag
     1621 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
     1681 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
     1741 cttgctcaca cctccgccag atgagccacc gccttccagt tttagaagct ccacatcgaa
     1801 gacgagagtg gcatgtggtg ggatgatgcc tgggtgccca gtggcaccat aggcataatc
     1861 tggagatata gtcagtttgg ctctctgacc cacactcatc tgggcaaccc cttcttccca
     1921 gcctcggatc acctcctgct tgcctagcat aaacttaaag ggcttgtttc tgtcccggga
     1981 ggaatcaaat ttctttccat cttcaagcat cccggtgtag tgcaccacgc aggtctggcc
     2041 gcgcttgggg aaggtgcgcc cgtctcctgg ggagatggtt tccacctgca ctccgccccg
     2101 ggacgcgtac gagatctgac ctccgccaga tgagccacct ccctgcaggc gcgccgtgta
     2161 cacgagctcc caattgggat ccccagcttg cagagcagct ctcgtagcca tttcaaaaac
     2221 ctctctcact ccatctttgg tctttgctga acactccatg tacccaaaag cgccaatcct
     2281 gtttgccata tctctgcctt cttcaggttt caccggctcc tgcttcatct tggctagctc
     2341 ccgccttgtg tgctcatcat tccgaagatc cttcttattc ccaaccagga tgatgggcac
     2401 gttgggacag aaatgcttga cttctggggt ccacttttct gggatgtttt ctaaactatc
     2461 agggctgtcg atggaaaaac acatcagtat aacatcggta tctgggtagg agaggggcct
     2521 caggcgatca taatcttcca gcccagctgt gtcccacaaa gccaactcta cctgctttcc
     2581 atccacctcg atatctgcca catagttctc aaacactgtg ggcacataca cctctgggaa
     2641 ctgatccttg ctgaagacta tgagcaagca tgtctttcca cacattctag aggatcccgg
     2701 gttgtggcaa gcttatcatc gtgtttttca aaggaaaacc acgtccccgt ggttcggggg
     2761 gcctagacgt ttttttaacc tcgactaaac acatgtaaag catgtgcacc gaggccccag
     2821 atcagatccc atacaatggg gtaccttctg ggcatccttc agccccttgt tgaatacgct
     2881 tgaggagagc catttgactc tttccacaac tatccaactc acaacgtggc actggggttg
     2941 tgccgccttt gcaggtgtat cttatacacg tggcttttgg ccgcagaggc acctgtcgcc
     3001 aggtgggggg ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc ttcaagaagc
     3061 ttccagagga actgcttcct tcacgacatt caacagacct tgcattcctt tggcgagagg
     3121 ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg ccctcacatt
     3181 gccaaaagac ggcaatatgg tggaaaataa catatagaca aacgcacacc ggccttattc
     3241 caagcggctt cggccagtaa cgttaggggg gggggaggga gagggggggg ggggagaggg
     3301 gcggaattgg ccgccctaga tgcatgctcg acgcgtgaat tcttaggctc catcaccaac
     3361 aatcaccagt ttcttccgga tggcagcgag ctcacctccg ccagatgagc caccgccccg
     3421 ggacgcgtac gagatctgct gctttgagat tcgtcggaac acatgataat agaggtccca
     3481 ggcttgggtg aggtccttga cattccctga tttcatgtac ttcctgcacc actcttgggc
     3541 ctccattaaa tctcgaccat aggcctgatt aaaggatgtt tccttcagag tctgggggcc
     3601 ccgttccatc atagcatgca agggctccag cacctcaaac atgcctttca cgttcctttc
     3661 cccaaagtac aaacgagatg cctcttccag gccttcatgc cacatctcac ctccgccaga
     3721 tgagccaccg cccttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
     3781 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt
     3841 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     3901 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttggc
     3961 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtcgctga tggcgttgta
     4021 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     4081 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     4141 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     4201 gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcacgcccc aggtcagggt
     4261 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     4321 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
     4381 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     4441 gctcaccatg ctagcctata gtgagtcgta ttaagtactc tagccttaag agctgtaatt
     4501 gaactgggag tggacacctg tggagagaaa ggcaaagtgg atgtcagtaa gaccaatagg
     4561 tgcctatcag aaacgcaaga gtcttctctg tctcgacaag cccagtttct attggtctcc
     4621 ttaaacctgt cttgtaacct tgatacttac ctgcccagtg cctcacgacc aacttctgca
     4681 aggctacctt aagagagtca ctgcagctta agttcgagac tgttgtgtca gaagcactga
     4741 ctgcgttagc aatttaactg tgataaacta ccgcaataaa gcttctagtg atctgacggt
     4801 tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     4861 tcaacggggc gggcgatcgc agttgttacg acattttgga aagtcccgtt gattttggtg
     4921 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     4981 ccgctatcca cgcccattgg tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     5041 taatacgtag atgtactgcc aagtaggaaa gtcccgtaag gtcatgtact gggcataatg
     5101 ccaggcgggc catttaccgt cattgacgtc aatagggggc ggacttggca tatgatacac
     5161 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     5221 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     5281 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     5341 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     5401 ccaacatggc ggtcatattg gacatgagcc aatataaatg tacatattat gatatagata
     5461 caacgtatgc aatggccaat agccaatatt gatttatgct atataaccaa tgaataatat
     5521 ggctaatggc caatattgaa gatctgtcga gccatgtgag caaaaggcca gcaaaaggcc
     5581 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5641 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5701 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5761 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5821 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5881 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5941 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6001 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6061 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6121 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6181 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6241 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6301 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6361 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6421 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6481 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6541 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6601 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6661 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6721 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6781 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6841 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6901 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6961 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7021 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7081 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7141 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7201 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7261 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7321 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     7381 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     7441 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     7501 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7561 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatacg
     7621 cggatcctta tcgctatcga ttcacacaaa aaaccaacac acagatgtaa tgaaaataaa
     7681 gatattttat tgcggccatc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     7741 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     7801 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     7861 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     7921 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     7981 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     8041 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     8101 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     8161 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     8221 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     8281 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     8341 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     8401 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     8461 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     8521 cctgcgtgca atccatcttg ttcaatcatg gtggctctag ccttaagttc gagactgttg
     8581 tgtcagaaga atcaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     8641 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     8701 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     8761 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     8821 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     8881 ttctgcctgc tg
//
