LOCUS       sequence-422246-        8895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express split-Rac1 fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C
  TITLE     Standardized Parts for Activation of Small GTPase Signaling in 
            Living Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 
            10.1002/anie.202403499.
  PUBMED    39058298
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499."
COMMENT     Sequence Label: 
            pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..8895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..70
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        361..740
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        741..952
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     903..923
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     949..973
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1113..1245
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1228..1247
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1290..1308
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1314..1316,1317..1319,1320..2030)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2055..2324
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     misc_feature    join(2471..3045,3046..3048,3049..3057)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2638..2655)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2865..2884
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3672..3992
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(4017..4019,4020..4730)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    4822..4943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4833..4852)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4887..4906
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5129..5584
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5216..5235)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5426..5447
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5701..6058
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5701..5892
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5724..5744)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5909..6044
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6109..6903
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6163..6182)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6773..6792
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6967..7015
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7056..7075)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7175..7197
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7235..7253)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7321..7425
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7426..7494,7495..8286)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7644..7663)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8457..8895,1..150)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
       61 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      121 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      181 ctggcctttt gctggccttt tgctcacatg gctcgacaga tcttcaatat tggccattag
      241 ccatattatt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
      301 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
      361 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      421 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      481 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      541 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      601 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      661 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      721 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      781 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      841 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctgcgatcgc ccgccccgtt
      901 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      961 gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa ttgctaacgc
     1021 agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agtgactctc ttaaggtagc
     1081 cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt
     1141 aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
     1201 cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt
     1261 tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct agcatggtga
     1321 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1381 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1441 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1501 ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     1561 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1621 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1681 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1741 agtacaacgc catcagcgac aacgtctata tcaccgccga caagcagaag aacggcatca
     1801 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1861 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1921 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1981 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtggct
     2041 catctggcgg aggtgagatg tggcatgaag gcctggaaga ggcatctcgt ttgtactttg
     2101 gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct atgatggaac
     2161 ggggccccca gactctgaag gaaacatcct ttaatcaggc ctatggtcga gatttaatgg
     2221 aggcccaaga gtggtgcagg aagtacatga aatcagggaa tgtcaaggac ctcacccaag
     2281 cctgggacct ctattatcat gtgttccgac gaatctcaaa gcagcagatc tcgtacgcgt
     2341 cccggggcgg tggctcatct ggcggaggtg agctccaggc catcaagtgt gtggtggtgg
     2401 gagacggagc ttaagaattc acgcgtcgag catgcatcta gggcggccaa ttccgcccct
     2461 ctcccccccc cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     2521 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     2581 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     2641 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     2701 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     2761 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     2821 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     2881 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     2941 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     3001 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccgg
     3061 gatcctctag aatggtaggt aaaaactgcc tactgatcag ttacacaacc aatgcatttc
     3121 ctggagaata tatccctact gtctttgaca attattctgc caatgttatg gtagatggaa
     3181 aaccggtgaa tctgggctta tgggatacag ctggacaaga agattatgac agattacgcc
     3241 ccctatccta tccgcaaaca gatgtgttct taatttgctt ttcccttgtg agtcctgcat
     3301 catttgaaaa tgtccgtgca aagtggtatc ctgaggtgcg gcaccactgt cccaacactc
     3361 ccatcatcct agtgggaact aaacttgatc ttagggatga taaagacacg atcgagaaac
     3421 tgaaggagaa gaagctgact cccatcacct atccgcaggg tctagccatg gctaaggaga
     3481 ttggtgctgt aaaatacctg gagtgctcgg cgctcacaca gcgaggcctc aagacagtgt
     3541 ttgacgaagc gatccgagca gtcctctgcc cgcctcccgt gggggatccc aattgggagc
     3601 tcgtgtacac ggcgcgcctg cagggaggtg gctcatctgg cggaggtcag atctcgtacg
     3661 cgtcccgggg cggagtgcag gtggaaacca tctccccagg agacgggcgc accttcccca
     3721 agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga aagaaatttg
     3781 attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag gaggtgatcc
     3841 gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa ctgactatat
     3901 ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca catgccactc
     3961 tcgtcttcga tgtggagctt ctaaaactgg aaggcggtgg ctcatctggc ggaggtgtga
     4021 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4081 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4141 tgaccctgaa gctcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4201 ccaccctcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     4261 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4321 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4381 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4441 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     4501 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     4561 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4621 gctaccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4681 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag aaaaaaaaga
     4741 agaaaaagag caagaccaaa tgcgtgatta tgtaagcggc cgcttccctt tagtgagggt
     4801 taatgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     4861 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     4921 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     4981 agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcc
     5041 gataaggatc gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5101 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     5161 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5221 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5281 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5341 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5401 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5461 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5521 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5581 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg
     5641 gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
     5701 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5761 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5821 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5881 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5941 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6001 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6061 gcttgattct tctgacacaa cagtctcgaa cttaaggcta gagccaccat gattgaacaa
     6121 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     6181 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     6241 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     6301 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6361 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     6421 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6481 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6541 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6601 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     6661 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6721 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6781 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6841 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6901 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat
     6961 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     7021 atagcgataa ggatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7081 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7141 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7201 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     7261 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     7321 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7381 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7441 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7501 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7561 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7621 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7681 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7741 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7801 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7861 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7921 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7981 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8041 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8101 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8161 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8221 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8281 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8341 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8401 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8461 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8521 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8581 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8641 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8701 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8761 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8821 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8881 aaggcggaca ggtat
//
