LOCUS       sequence-422247-        8895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express split-Rac1 fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C
  TITLE     Standardized Parts for Activation of Small GTPase Signaling in 
            Living Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 
            10.1002/anie.202403499.
  PUBMED    39058298
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499."
COMMENT     Sequence Label: 
            pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..8895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      145..280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             345..1139
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(399..418)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1009..1028
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1203..1251
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(1292..1311)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1411..1433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1471..1489)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1557..1661
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1662..1730,1731..2522)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1880..1899)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2693..3281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3182..3201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3492..3871
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3872..4083
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4034..4054
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4080..4104
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4244..4376
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4359..4378
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4421..4439
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4445..4447,4448..4450,4451..5161)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5186..5455
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     misc_feature    join(5602..6176,6177..6179,6180..6188)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5769..5786)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5996..6015
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6803..7123
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(7148..7150,7151..7861)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    7953..8074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7964..7983)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8018..8037
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      8260..8715
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8347..8366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8557..8578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(8832..8895,1..294)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(8832..8895,1..128)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8855..8875)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      181 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
      241 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
      301 gattcttctg acacaacagt ctcgaactta aggctagagc caccatgatt gaacaagatg
      361 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
      421 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
      481 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
      541 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
      601 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
      661 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
      721 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
      781 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
      841 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
      901 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
      961 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     1021 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     1081 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     1141 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgatggcc
     1201 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     1261 cgataaggat ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     1321 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     1381 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     1441 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     1501 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     1561 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1621 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1681 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1741 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1801 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1861 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1921 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1981 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2041 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2101 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2161 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2221 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2281 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2341 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2401 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2461 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2521 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2581 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2641 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2701 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2761 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2821 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2881 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2941 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3001 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3061 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3121 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3181 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3241 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3301 ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag atcttcaata
     3361 ttggccatta gccatattat tcattggtta tatagcataa atcaatattg gctattggcc
     3421 attgcatacg ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg
     3481 accgccatgt tggcattgat tattgactag ttattaatag taatcaatta cggggtcatt
     3541 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     3601 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     3661 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     3721 ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa
     3781 atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta
     3841 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg
     3901 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     3961 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actgcgatcg
     4021 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     4081 gctcgtttag tgaaccgtca gatcactaga agctttattg cggtagttta tcacagttaa
     4141 attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg cagtgactct
     4201 cttaaggtag ccttgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac
     4261 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
     4321 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
     4381 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc
     4441 tagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4501 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     4561 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4621 caccctcgtg accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat
     4681 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4741 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4801 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4861 gcacaagctg gagtacaacg ccatcagcga caacgtctat atcaccgccg acaagcagaa
     4921 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4981 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     5041 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     5101 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     5161 gggcggtggc tcatctggcg gaggtgagat gtggcatgaa ggcctggaag aggcatctcg
     5221 tttgtacttt ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc
     5281 tatgatggaa cggggccccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg
     5341 agatttaatg gaggcccaag agtggtgcag gaagtacatg aaatcaggga atgtcaagga
     5401 cctcacccaa gcctgggacc tctattatca tgtgttccga cgaatctcaa agcagcagat
     5461 ctcgtacgcg tcccggggcg gtggctcatc tggcggaggt gagctccagg ccatcaagtg
     5521 tgtggtggtg ggagacggag cttaagaatt cacgcgtcga gcatgcatct agggcggcca
     5581 attccgcccc tctccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag
     5641 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     5701 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     5761 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     5821 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     5881 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     5941 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     6001 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6061 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6121 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg
     6181 ccacaacccg ggatcctcta gaatggtagg taaaacttgc ctactgatca gttacacaac
     6241 caatgcattt cctggagaat atatccctac tgtctttgac aattattctg ccaatgttat
     6301 ggtagatgga aaaccggtga atctgggctt atgggataca gctggactag aagattatga
     6361 cagattacgc cccctatcct atccgcaaac agatgtgttc ttaatttgct tttcccttgt
     6421 gagtcctgca tcatttgaaa atgtccgtgc aaagtggtat cctgaggtgc ggcaccactg
     6481 tcccaacact cccatcatcc tagtgggaac taaacttgat cttagggatg ataaagacac
     6541 gatcgagaaa ctgaaggaga agaagctgac tcccatcacc tatccgcagg gtctagccat
     6601 ggctaaggag attggtgctg taaaatacct ggagtgctcg gcgctcacac agcgaggcct
     6661 caagacagtg tttgacgaag cgatccgagc agtcctctgc ccgcctcccg tgggggatcc
     6721 caattgggag ctcgtgtaca cggcgcgcct gcagggaggt ggctcatctg gcggaggtca
     6781 gatctcgtac gcgtcccggg gcggagtgca ggtggaaacc atctccccag gagacgggcg
     6841 caccttcccc aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg
     6901 aaagaaattt gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca
     6961 ggaggtgatc cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa
     7021 actgactata tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc
     7081 acatgccact ctcgtcttcg atgtggagct tctaaaactg gaaggcggtg gctcatctgg
     7141 cggaggtgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     7201 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     7261 ctacggcaag ctgaccctga agctcatctg caccaccggc aagctgcccg tgccctggcc
     7321 caccctcgtg accaccctcg gctacggcct gcagtgcttc gcccgctacc ccgaccacat
     7381 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     7441 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     7501 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     7561 gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa
     7621 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
     7681 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     7741 ccactacctg agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     7801 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     7861 gaaaaaaaag aagaaaaaga gcaagaccaa atgcgtgatt atgtaagcgg ccgcttccct
     7921 ttagtgaggg ttaatgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     7981 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     8041 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     8101 gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     8161 tggtaaaatc cgataaggat cgatccgggc tggcgtaata gcgaagaggc ccgcaccgat
     8221 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat
     8281 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8341 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8401 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8461 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8521 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8581 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8641 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     8701 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8761 ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
     8821 ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     8881 aagtccccag gctcc
//
