LOCUS       sequence-422248-        8904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express split-Cdc42 fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C
  TITLE     Standardized Parts for Activation of Small GTPase Signaling in 
            Living Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 
            10.1002/anie.202403499.
  PUBMED    39058298
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499."
COMMENT     Sequence Label: 
            pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..8904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        356..735
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        736..947
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     898..918
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     944..968
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1108..1240
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1223..1242
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1285..1303
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1309..1311,1312..1314,1315..2025)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2050..2319
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     misc_feature    join(2466..3040,3041..3043,3044..3052)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2633..2650)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2860..2879
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3676..3996
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(4021..4023,4024..4734)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    4826..4947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4837..4856)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4891..4910
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5133..5588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5220..5239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5430..5451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5705..6062
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5705..5896
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5728..5748)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5913..6048
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6113..6907
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6167..6186)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6777..6796
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6971..7019
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7060..7079)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7179..7201
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7239..7257)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7325..7429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7430..7498,7499..8290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7648..7667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8461..8904,1..145)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
       61 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      121 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      181 cttttgctgg ccttttgctc acatggctcg acagatcttc aatattggcc attagccata
      241 ttattcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
      301 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttggcat
      361 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      421 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      481 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      541 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      601 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      661 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
      721 atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt
      781 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      841 caaaatcaac gggactttcc aaaatgtcgt aacaactgcg atcgcccgcc ccgttgacgc
      901 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      961 gtcagatcac tagaagcttt attgcggtag tttatcacag ttaaattgct aacgcagtca
     1021 gtgcttctga cacaacagtc tcgaacttaa gctgcagtga ctctcttaag gtagccttgc
     1081 agaagttggt cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga
     1141 gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct
     1201 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat
     1261 tacagctctt aaggctagag tacttaatac gactcactat aggctagcat ggtgagcaag
     1321 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1381 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1441 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1501 ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     1561 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1621 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1681 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1741 aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     1801 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1861 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1921 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1981 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg tggctcatct
     2041 ggcggaggtg agatgtggca tgaaggcctg gaagaggcat ctcgtttgta ctttggggaa
     2101 aggaacgtga aaggcatgtt tgaggtgctg gagcccttgc atgctatgat ggaacggggc
     2161 ccccagactc tgaaggaaac atcctttaat caggcctatg gtcgagattt aatggaggcc
     2221 caagagtggt gcaggaagta catgaaatca gggaatgtca aggacctcac ccaagcctgg
     2281 gacctctatt atcatgtgtt ccgacgaatc tcaaagcagc agatctcgta cgcgtcccgg
     2341 ggcggtggct catctggcgg aggtgagctc cagacaatta agtgtgttgt tgtgggcgat
     2401 ggtgcttaag aattcacgcg tcgagcatgc atctagggcg gccaattccg cccctctccc
     2461 ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     2521 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     2581 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     2641 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     2701 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     2761 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     2821 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     2881 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     2941 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     3001 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccgggatcc
     3061 tctagaatgg gggttggtaa aaactgtctc ctgatatcct acacaacaaa caaatttcca
     3121 tcggagtatg taccgactgt ttttgacaac tatgcagtca cagttatgat tggtggagaa
     3181 ccatatactc ttggactttt tgatactgca gggcaagagg attatgacag attacgaccg
     3241 ctgagttatc cacaaacaga tgtatttcta gtctgttttt cagtggtctc tccatcttca
     3301 tttgaaaacg tgaaagaaaa gtgggtgcct gagataactc accactgtcc aaagactcct
     3361 ttcttgcttg ttgggactca aattgatctc agagatgacc cctctactat tgagaaactt
     3421 gccaagaaca aacagaagcc tatcactcca gagactgctg aaaagctggc ccgtgacctg
     3481 aaggctgtca agtatgtgga gtgttctgca cttacacaga gaggtctgaa gaatgtgttt
     3541 gatgaggcta tcctagctgc cctcgagcct ccggaaactc aacccgggga tcccaattgg
     3601 gagctcgtgt acacggcgcg cctgcaggga ggtggctcat ctggcggagg tcagatctcg
     3661 tacgcgtccc ggggcggagt gcaggtggaa accatctccc caggagacgg gcgcaccttc
     3721 cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa
     3781 tttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg
     3841 atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact
     3901 atatctccag attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc
     3961 actctcgtct tcgatgtgga gcttctaaaa ctggaaggcg gtggctcatc tggcggaggt
     4021 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4081 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4141 aagctgaccc tgaagctcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4201 gtgaccaccc tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     4261 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4321 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4381 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4441 ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     4501 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     4561 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4621 ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4681 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaaaaaa
     4741 aagaagaaaa agagcaagac caaatgcgtg attatgtaag cggccgcttc cctttagtga
     4801 gggttaatgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     4861 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4921 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     4981 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     5041 atccgataag gatcgatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt
     5101 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc
     5161 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5221 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5281 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5341 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5401 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5461 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5521 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5581 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     5641 cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta
     5701 ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5761 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5821 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5881 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5941 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6001 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6061 aaaagcttga ttcttctgac acaacagtct cgaacttaag gctagagcca ccatgattga
     6121 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6181 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6241 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6301 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6361 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6421 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6481 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6541 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6601 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     6661 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6721 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6781 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6841 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6901 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     6961 cgatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     7021 atcgatagcg ataaggatcc gcgtatggtg cactctcagt acaatctgct ctgatgccgc
     7081 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     7141 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     7201 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     7261 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7321 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7381 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7441 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7501 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7561 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7621 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7681 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7741 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7801 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7861 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7921 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7981 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8041 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8101 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8161 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8221 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8281 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8341 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8461 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8521 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8581 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8641 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8701 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8761 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8821 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8881 gagaaaggcg gacaggtatc cggt
//
