LOCUS       sequence-422249-        8904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express split-Cdc42 fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C
  TITLE     Standardized Parts for Activation of Small GTPase Signaling in 
            Living Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 
            10.1002/anie.202403499.
  PUBMED    39058298
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499."
COMMENT     Sequence Label: 
            pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..8904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        199..578
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        579..790
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     741..761
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     787..811
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          951..1083
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1066..1085
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1128..1146
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1152..1154,1155..1157,1158..1868)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1893..2162
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     misc_feature    join(2309..2883,2884..2886,2887..2895)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2476..2493)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2703..2722
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3519..3839
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(3864..3866,3867..4577)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    4669..4790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4680..4699)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4734..4753
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4976..5431
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5063..5082)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5273..5294
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5548..5905
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5548..5739
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5571..5591)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5756..5891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5956..6750
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6010..6029)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6620..6639
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6814..6862
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6903..6922)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7022..7044
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7082..7100)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7168..7272
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7273..7341,7342..8133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7491..7510)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8304..8892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8793..8812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc tcgacagatc
       61 ttcaatattg gccattagcc atattattca ttggttatat agcataaatc aatattggct
      121 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
      181 caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa tcaattacgg
      241 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      301 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      361 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      421 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
      481 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
      541 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      601 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      661 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      721 gcgatcgccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      781 aagcagagct cgtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca
      841 cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag
      901 tgactctctt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca
      961 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     1021 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     1081 caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac
     1141 tataggctag catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1201 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1261 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1321 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcgcc cgctaccccg
     1381 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1441 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1501 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1561 tcctggggca caagctggag tacaacgcca tcagcgacaa cgtctatatc accgccgaca
     1621 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg
     1681 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1741 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     1801 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1861 tgtacaaggg cggtggctca tctggcggag gtgagatgtg gcatgaaggc ctggaagagg
     1921 catctcgttt gtactttggg gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct
     1981 tgcatgctat gatggaacgg ggcccccaga ctctgaagga aacatccttt aatcaggcct
     2041 atggtcgaga tttaatggag gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg
     2101 tcaaggacct cacccaagcc tgggacctct attatcatgt gttccgacga atctcaaagc
     2161 agcagatctc gtacgcgtcc cggggcggtg gctcatctgg cggaggtgag ctccagacaa
     2221 ttaagtgtgt tgttgtgggc gatggtgctt aagaattcac gcgtcgagca tgcatctagg
     2281 gcggccaatt ccgcccctct cccccccccc cctctccctc ccccccccct aacgttactg
     2341 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     2401 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     2461 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     2521 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     2581 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     2641 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     2701 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     2761 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     2821 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     2881 aagcttgcca caacccggga tcctctagaa tgggggttgg taaaacatgt ctcctgatat
     2941 cctacacaac aaacaaattt ccatcggagt atgtaccgac tgtttttgac aactatgcag
     3001 tcacagttat gattggtgga gaaccatata ctcttggact ttttgatact gcagggctag
     3061 aggattatga cagattacga ccgctgagtt atccacaaac agatgtattt ctagtctgtt
     3121 tttcagtggt ctctccatct tcatttgaaa acgtgaaaga aaagtgggtg cctgagataa
     3181 ctcaccactg tccaaagact cctttcttgc ttgttgggac tcaaattgat ctcagagatg
     3241 acccctctac tattgagaaa cttgccaaga acaaacagaa gcctatcact ccagagactg
     3301 ctgaaaagct ggcccgtgac ctgaaggctg tcaagtatgt ggagtgttct gcacttacac
     3361 agagaggtct gaagaatgtg tttgatgagg ctatcctagc tgccctcgag cctccggaaa
     3421 ctcaacccgg ggatcccaat tgggagctcg tgtacacggc gcgcctgcag ggaggtggct
     3481 catctggcgg aggtcagatc tcgtacgcgt cccggggcgg agtgcaggtg gaaaccatct
     3541 ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg cactacaccg
     3601 ggatgcttga agatggaaag aaatttgatt cctcccggga cagaaacaag ccctttaagt
     3661 ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc cagatgagtg
     3721 tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc actgggcacc
     3781 caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta aaactggaag
     3841 gcggtggctc atctggcgga ggtgtgagca agggcgagga gctgttcacc ggggtggtgc
     3901 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     3961 gcgagggcga tgccacctac ggcaagctga ccctgaagct catctgcacc accggcaagc
     4021 tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta cggcctgcag tgcttcgccc
     4081 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4141 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4201 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4261 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     4321 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     4381 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4441 tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg
     4501 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     4561 tggacgagct gtacaagaaa aaaaagaaga aaaagagcaa gaccaaatgc gtgattatgt
     4621 aagcggccgc ttccctttag tgagggttaa tgcttcgagc agacatgata agatacattg
     4681 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4741 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4801 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt
     4861 aaaacctcta caaatgtggt aaaatccgat aaggatcgat ccgggctggc gtaatagcga
     4921 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
     4981 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5041 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5101 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5161 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5221 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5281 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5341 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5401 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     5461 tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca ccatggcctg aaataacctc
     5521 tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg
     5581 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5641 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5701 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5761 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5821 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5881 ttttggaggc ctaggctttt gcaaaaagct tgattcttct gacacaacag tctcgaactt
     5941 aaggctagag ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     6001 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     6061 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6121 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     6181 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6241 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6301 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6361 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6421 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6481 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     6541 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     6601 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6661 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6721 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     6781 aagcgacgcc caacctgcca tcacgatggc cgcaataaaa tatctttatt ttcattacat
     6841 ctgtgtgttg gttttttgtg tgaatcgata gcgataagga tccgcgtatg gtgcactctc
     6901 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     6961 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7021 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     7081 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     7141 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7201 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7261 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7321 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7381 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7441 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7501 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7561 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7621 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7681 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7741 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7801 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7861 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7921 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7981 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8041 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8101 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8161 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8221 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8281 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8341 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8401 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8461 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8521 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8581 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8641 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8701 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8761 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8821 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8881 agcctatgga aaaacgccag caac
//
