LOCUS       sequence-422263-       13110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti-viral expression of human androgen receptor wild-type (WT)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13110)
  AUTHORS   Guo H, Wu Y, Nouri M, Spisak S, Russo JW, Sowalsky AG, Pomerantz MM,
            Wei Z, Korthauer K, Seo JH, Wang L, Arai S, Freedman ML, He HH, Chen
            S, Balk SP
  TITLE     Androgen receptor and MYC equilibration centralizes on developmental
            super-enhancer.
  JOURNAL   Nat Commun. 2021 Dec 15;12(1):7308. doi: 10.1038/s41467-021-27077-y.
  PUBMED    34911936
REFERENCE   2  (bases 1 to 13110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-15"; volume: "12(1):7308. doi"; pages: " 
            10.1038/s41467-021-27077-y"
COMMENT     Sequence Label: LV-AR
FEATURES             Location/Qualifiers
     source          1..13110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11..76
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2828..2881
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2882..3907
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    3932..4520
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3985..4005)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4403..4414)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4523..4539)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4524..4540)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5045..5225
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5251..5268)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5257..5481
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5527..5955
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5614..5633)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5824..5845
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5964..5984)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5969..6298
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6149..6284
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6211..6230
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6346..6393
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6412..6786
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6916..7037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6953..6972)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7007..7026
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7086..7102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7086..7102)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7099..7121)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7110..7126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7134..7140,7141..7158,7159..7164))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7179..7200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7317..7334)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7488..8076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7568..7587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8247..9038,9039..9107))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8870..8889
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9108..9212)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9287..9306)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9478..9857
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9859..10057
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10012..10032
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10075..10255
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10302..10427
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10920..11153
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11338..11382
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11680..11797
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        11927..13105
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          12158..13096
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     13053..13073
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggccaccatg gactataagg accacgacgg agactacaag gatcatgata ttgattacaa
       61 agacgatgac gataagatgg cccgtacggc caccatggaa gtgcagttag ggctgggaag
      121 ggtctaccct cggccgccgt ccaagaccta ccgaggagct ttccagaatc tgttccagag
      181 cgtgcgcgaa gtgatccaga acccgggccc caggcaccca gaggccgcga gcgcagcacc
      241 tcccggcgcc agtttgctgc tgctgcagca gcagcagcag cagcagcagc agcagcagca
      301 gcagcagcag cagcaagaga ctagccccag gcagcagcag cagcagcagg gtgaggatgg
      361 ttctccccaa gcccatcgta gaggccccac aggctacctg gtcctggatg aggaacagca
      421 accttcacag ccgcagtcgg ccctggagtg ccaccccgag agaggttgcg tcccagagcc
      481 tggagccgcc gtggccgcca gcaaggggct gccgcagcag ctgccagcac ctccggacga
      541 ggatgactca gctgccccat ccacgttgtc cctgctgggc cccactttcc ccggcttaag
      601 cagctgctcc gctgacctta aagacatcct gagcgaggcc agcaccatgc aactccttca
      661 gcaacagcag caggaagcag tatccgaagg cagcagcagc gggagagcga gggaggcctc
      721 gggggctccc acttcctcca aggacaatta cttagggggc acttcgacca tttctgacaa
      781 cgccaaggag ttgtgtaagg cagtgtcggt gtccatgggc ctgggtgtgg aggcgttgga
      841 gcatctgagt ccaggggaac agcttcgggg ggattgcatg tacgccccac ttttgggagt
      901 tccacccgct gtgcgtccca ctccttgtgc cccattggcc gaatgcaaag gttctctgct
      961 agacgacagc gcaggcaaga gcactgaaga tactgctgag tattcccctt tcaagggagg
     1021 ttacaccaaa gggctagaag gcgagagcct aggctgctct ggcagcgctg cagcagggag
     1081 ctccgggaca cttgaactgc cgtctaccct gtctctctac aagtccggag cactggacga
     1141 ggcagctgcg taccagagtc gcgactacta caactttcca ctggctctgg ccggaccgcc
     1201 gccccctccg ccgcctcccc atccccacgc tcgcatcaag ctggagaacc cgctggacta
     1261 cggcagcgcc tgggcggctg cggcggcgca gtgccgctat ggggacctgg cgagcctgca
     1321 tggcgcgggt gcagcgggac ccggttctgg gtcaccctca gccgccgctt cctcatcctg
     1381 gcacactctc ttcacagccg aagaaggcca gttgtatgga ccgtgtggtg gtggtggggg
     1441 tggtggtggc ggcggcggcg gcggcggcgg cgaggcggga gctgtagccc cctacggcta
     1501 cactcggccc cctcaggggc tggcgggcca ggaaagcgac ttcaccgcac ctgatgtgtg
     1561 gtaccctggc ggcatggtga gcagagtgcc ctatcccagt cccacttgtg tcaaaagcga
     1621 aatgggcccc tggatggata gctactccgg accttacggg gacatgcgtt tggagactgc
     1681 cagggaccat gttttgccca ttgactatta ctttccaccc cagaagacct gcctgatctg
     1741 tggagatgaa gcttctgggt gtcactatgg agctctcaca tgtggaagct gcaaggtctt
     1801 cttcaaaaga gccgctgaag ggaaacagaa gtacctgtgc gccagcagaa atgattgcac
     1861 tattgataaa ttccgaagga aaaattgtcc atcttgtcgt cttcggaaat gttatgaagc
     1921 agggatgact ctgggagccc ggaagctgaa gaaacttggt aatctgaaac tacaggagga
     1981 aggagaggct tccagcacca ccagccccac tgaggagaca acccagaagc tgacagtgtc
     2041 acacattgaa ggctatgaat gtcagcccat ctttctgaat gtcctggaag ccattgagcc
     2101 aggtgtagtg tgtgctggac acgacaacaa ccagcccgac tcctttgcag ccttgctctc
     2161 tagcctcaat gaactgggag agagacagct tgtacacgtg gtcaagtggg ccaaggcctt
     2221 gcctggcttc cgcaacttac acgtggacga ccagatggct gtcattcagt actcctggat
     2281 ggggctcatg gtgtttgcca tgggctggcg atccttcacc aatgtcaact ccaggatgct
     2341 ctacttcgcc cctgatctgg ttttcaatga gtaccgcatg cacaagtccc ggatgtacag
     2401 ccagtgtgtc cgaatgaggc acctctctca agagtttgga tggctccaaa tcacccccca
     2461 ggaattcctg tgcatgaaag cactgctact cttcagcatt attccagtgg atgggctgaa
     2521 aaatcaaaaa ttctttgatg aacttcgaat gaactacatc aaggaactcg atcgtatcat
     2581 tgcatgcaaa agaaaaaatc ccacatcctg ctcaagacgc ttctaccagc tcaccaagct
     2641 cctggactcc gtgcagccta ttgcgagaga gctgcatcag ttcacttttg acctgctaat
     2701 caagtcacac atggtgagcg tggactttcc ggaaatgatg gcagagatca tctctgtgca
     2761 agtgcccaag atcctttctg ggaaagtcaa gcccatctat ttccacaccc agggatccgg
     2821 cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc
     2881 aatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     2941 cagcgtgtcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     3001 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     3061 tcgttatgtt catcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     3121 tggggaattt agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     3181 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     3241 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     3301 aatcggtcaa tacactacgt ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     3361 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     3421 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     3481 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     3541 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     3601 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     3661 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     3721 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     3781 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     3841 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     3901 ggaataagaa ttcgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga
     3961 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4021 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4081 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4141 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4201 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4261 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4321 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4381 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4441 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4501 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     4561 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     4621 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     4681 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4741 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     4801 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     4861 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     4921 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     4981 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     5041 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5101 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5161 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5221 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     5281 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5341 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5401 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5461 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     5521 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5581 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5641 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5701 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5761 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5821 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5881 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5941 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     6001 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     6061 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6121 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6181 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     6241 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6301 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     6361 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     6421 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     6481 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     6541 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     6601 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     6661 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     6721 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     6781 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     6841 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6901 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7021 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     7081 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7141 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7201 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7261 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7381 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7441 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7501 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7561 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7621 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7681 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7741 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7801 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7861 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7921 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7981 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8041 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8101 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8161 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8221 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8281 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8341 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8401 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8461 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8521 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8581 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8641 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8701 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8761 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8821 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8881 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8941 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9001 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9061 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9121 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9181 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9241 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     9301 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     9361 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     9421 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     9481 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     9541 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     9601 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     9661 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     9721 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     9781 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     9841 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     9901 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     9961 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
    10021 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
    10081 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    10141 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10201 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
    10261 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
    10321 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
    10381 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
    10441 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
    10501 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
    10561 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    10621 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    10681 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    10741 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
    10801 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    10861 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    10921 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    10981 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    11041 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    11101 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    11161 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    11221 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    11281 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    11341 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    11401 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    11461 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    11521 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
    11581 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
    11641 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
    11701 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
    11761 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
    11821 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
    11881 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
    11941 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
    12001 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
    12061 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
    12121 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
    12181 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
    12241 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
    12301 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
    12361 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
    12421 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
    12481 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
    12541 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
    12601 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
    12661 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
    12721 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
    12781 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
    12841 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
    12901 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
    12961 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
    13021 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
    13081 ttttcttcca tttcaggtgt cgtgacgtac
//
