LOCUS       sequence-422274-       11247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant transient expression cassette for the E3-DART ligase under a 
            UBQ10 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 11247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-...
FEATURES             Location/Qualifiers
     source          1..11247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2011..2037
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2159..2170
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      4419..4671
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4706..4724)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    4912..4936
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6237..6866
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7295..8368
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8434..8628
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8864..8886)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8972..9112
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9027..9046
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9127..9144)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9298..9886)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9378..9397)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9974..10789)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9974..10789)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10697..10716
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    11222..11246
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacgaattc gtctcaggag agtctagctc aacagagctt ttaacccaaa ttggtacaat
       61 agaatacaac tttagatcat aattctcaaa agaaagagat tccttagcta ttctatctgc
      121 cactccattt ccttctcggc ttgtatgcac aagcataaaa tcctcaaact tgctaagtag
      181 atactttatg tcttggataa ttggattgag acttgacaag cataactttc atgtaaccaa
      241 agacacaagt tgctgagaat ccacctcaaa aatgatcttc ctataattga atcgggataa
      301 tgacagcaca gcccatctaa gagcctccac ttctacttcc agcacgcttc ttacttttac
      361 cacagctctt gcacctaacc ataacacctt ccctgtatga tcgcgaagca cccaccctaa
      421 gccacatttt aatccttctg ttggccatgc cccatcaaag ttgcacttaa cccaagattg
      481 tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta
      541 cattctgagc ctctttcctt ctaatccact catctgcatc ttcttgtgtc cttactaata
      601 cctcattggt tccaaattcc ctccctttaa gcaccagctc gtttctgttc ttccacagcc
      661 tcccaagtat ccaagggact aaagcctcca cattcttcag atcaggatat tcttgtttaa
      721 gatgttgaac tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt
      781 cttcatagcg aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa
      841 tgaaaaaata ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa
      901 ataagatcca ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg
      961 ccttttttaa cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca
     1021 ataatcatac aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa
     1081 tatgttatac gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt
     1141 gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta
     1201 gaaaatacga aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca
     1261 gctccaccgt atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat
     1321 cgttaaatct caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt
     1381 cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc
     1441 aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg
     1501 tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc
     1561 tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa
     1621 agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc
     1681 aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga
     1741 ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt
     1801 agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt
     1861 ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg
     1921 atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga
     1981 ttaatctgag tttttctgat taacagaatg tacccatacg atgttccaga ttacgctgag
     2041 tatgatgctg tctggtcaaa atgggaaagg gatgcaccag caggagagtc acctggaaga
     2101 gcagcagtgg ttcaggaaat gcgtgattgc cttaataacg gaaatccagt gcttaacgtg
     2161 ggagcttcag gtcttaccac ccttccagac cgtcttccac cgcatattac aacacttgtt
     2221 attccagata ataatctcac ctctttgcct gagttgccag aaggacttag agagcttgag
     2281 gtgtctggaa accttcagct tacatctctt ccatctcttc cgcagggtct tcagaagttg
     2341 tgggcctata acaattggct tgcctctctt ccgactttgc cgccaggact tggggatctt
     2401 gctgtctcta ataaccagct tacctctctt ccggagatgc cgccagcact tagagagctt
     2461 agggtgtctg gtaacaacct tacctctctt ccggctcttc cgtcaggact tcagaagctt
     2521 tgggcatata acaatagatt gacctctttg ccagagatgt caccaggtct tcaggaactt
     2581 gatgtctctc ataatcagct taccagattg ccgcaatctc tcactggtct ttcttctgct
     2641 gcaagagttt atcttgacgg gaatccactt tctgttagaa ctcttcaggc tcttagagac
     2701 atcattggac attcaggaat caggatacac ttcgatatgg ctgggccttc tgtccctaga
     2761 gaagccagag cacttcacct tgctgtcgct gactggctta cttctgcaag agagggggaa
     2821 gctgcccagg cagacagatg gcaggctttc ggacttgaag ataacgccgc cgccttctct
     2881 cttgtccttg acagacttcg tgagactgaa aacttcaaaa aagacgctgg atttaaggca
     2941 cagatatcat cttggcttac acaacttgct gaagatgctg ctcttagagc aaaaaccttt
     3001 gccatggcaa cagaggcaac atcaaccgct gaggacagag tcacacatgc ccttcaccag
     3061 atgaataacg ttcaacttgt tcataatgca gaaaaagggg aatacgacaa caatctccag
     3121 gggcttgttt ctactgggcg tgagatgttc agacttgcaa cacttgaaca gattgccaga
     3181 gaaaaagccg gaacacttgc tcttgtcgat gacgttgagg tctatcttgc tttccagaat
     3241 aagcttaagg aatcacttga gcttacctct gtgacttcag aaatgcgttt ctttgacgtt
     3301 tctggagtga ctgtttcaga ccttcaggct gctgagcttc aggtgaaaac cgctgaaaac
     3361 tctgggttca gtaaatggat acttcagtgg gggccgcttc actctgtgct tgaaagaaaa
     3421 gtgccggaaa gatttaacgc tcttcgtgaa aagcaaatat ctgattatga agacacttac
     3481 agaaagcttt atgacgaagt gcttaaatct tctgggcttg tcgacgatac cgatgcagaa
     3541 cgtactatcg gagttagtgc tatggatagt gctaaaaaag aatttcttga tggacttaga
     3601 gctcttgtgg atgaggtgct tggttcttat cttacagcca gatggcgtct taacttcgga
     3661 ggaggtggct catctggcgg aggtatggtg agtaagggtg aagaagataa tatggcaata
     3721 attaaagaat ttatgcgttt taaagtccat atggaaggat cagtcaatgg tcatgaattc
     3781 gaaattgagg gggaaggtga aggaaggccg tatgagggga ctcagaccgc taaactcaag
     3841 gtgaccaagg gtgggccttt gccgtttgca tgggatatcc tctctccaca atttatgtat
     3901 ggatcaaagg cttacgtcaa gcacccggct gacatacctg actatcttaa actctctttc
     3961 ccggaaggtt ttaagtggga aagagtgatg aatttcgaag atggtggggt tgtgaccgtt
     4021 acacaagatt caagtctgca ggatggagag tttatttaca aggttaaact cagaggtacc
     4081 aatttcccgt ctgacgggcc tgtgatgcaa aaaaagacta tggggtggga ggcctcttca
     4141 gaaagaatgt atcctgagga tggagcactc aagggtgaaa ttaaacaaag attgaagctt
     4201 aaagatggag gtcattatga cgccgaagtg aaaactacat ataaggcaaa gaagccagtc
     4261 caattgccgg gagcatataa tgtgaatatt aaactcgata tcacctctca caatgaagat
     4321 tatactatcg tcgaacagta tgaaagggcc gaaggtcgtc attcaaccgg aggtatggat
     4381 gagctctata agtgagcttg gaatggatct tcgatcccga tcgttcaaac atttggcaat
     4441 aaagtttctt aagattgaat cctgttgccg gtcttgcgac gattatcata taatttctgt
     4501 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     4561 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     4621 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgc
     4681 caagctaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4741 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4801 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4861 tgataaatgc ttcaataatg ggaccgactc gcgctgtcat gagacgaatt ctgacaggat
     4921 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     4981 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     5041 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     5101 ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     5161 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     5221 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     5281 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     5341 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     5401 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     5461 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     5521 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     5581 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     5641 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
     5701 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
     5761 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
     5821 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
     5881 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
     5941 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
     6001 atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct caaccgtgcg gctgcatgaa
     6061 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
     6121 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
     6181 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
     6241 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
     6301 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
     6361 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
     6421 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
     6481 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
     6541 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
     6601 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
     6661 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
     6721 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
     6781 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
     6841 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
     6901 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
     6961 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
     7021 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
     7081 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
     7141 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
     7201 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca
     7261 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
     7321 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
     7381 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     7441 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     7501 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     7561 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     7621 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
     7681 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
     7741 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
     7801 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
     7861 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
     7921 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
     7981 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
     8041 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
     8101 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
     8161 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
     8221 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
     8281 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
     8341 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
     8401 agcaggggaa aaaggtcgaa aaggactctt tcctgtggat agcacgtaca ttgggaaccc
     8461 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
     8521 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
     8581 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
     8641 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
     8701 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
     8761 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
     8821 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     8881 tcccggtgac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     8941 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     9001 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     9061 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     9121 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9181 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9241 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9301 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9361 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9421 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9481 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9541 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9601 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9661 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9721 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9781 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9841 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9901 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9961 gcattctagg tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
    10021 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
    10081 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
    10141 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
    10201 catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt
    10261 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
    10321 tcattcgtga ttgcgcctga gcgagtcgaa atacgcgatc gctgttaaaa ggacaattac
    10381 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
    10441 ctgaatcagg atattcttct aatacctgga atgctgtttt ccctgggatc gcagtggtga
    10501 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
    10561 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
    10621 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcggtag attgtcgcac
    10681 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
    10741 aatttaatcg cggccttgag caagacgttt cccgttgaat atggctcata acagaactta
    10801 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    10861 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    10921 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
    10981 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    11041 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    11101 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    11161 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    11221 gtggcaggat atattgtggt gtaaaca
//
