LOCUS       sequence-422275-        9519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant transient expression cassette for HR1b-GFP fusion under UBQ10 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S
FEATURES             Location/Qualifiers
     source          1..9519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2251..2253,2254..2256,2257..2970)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2251..2253,2254..2256,2257..2967)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2296..2317)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2557..2576)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2904..2925
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3184..3208
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4509..5138
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5567..6640
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6706..6900
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7136..7158)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7244..7384
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7299..7318
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7399..7416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7570..8158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7650..7669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8246..9061)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8246..9061)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8969..8988
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9494..9518
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacgaattc gtctcaggag agtctagctc aacagagctt ttaacccaaa ttggtacaat
       61 agaatacaac tttagatcat aattctcaaa agaaagagat tccttagcta ttctatctgc
      121 cactccattt ccttctcggc ttgtatgcac aagcataaaa tcctcaaact tgctaagtag
      181 atactttatg tcttggataa ttggattgag acttgacaag cataactttc atgtaaccaa
      241 agacacaagt tgctgagaat ccacctcaaa aatgatcttc ctataattga atcgggataa
      301 tgacagcaca gcccatctaa gagcctccac ttctacttcc agcacgcttc ttacttttac
      361 cacagctctt gcacctaacc ataacacctt ccctgtatga tcgcgaagca cccaccctaa
      421 gccacatttt aatccttctg ttggccatgc cccatcaaag ttgcacttaa cccaagattg
      481 tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta
      541 cattctgagc ctctttcctt ctaatccact catctgcatc ttcttgtgtc cttactaata
      601 cctcattggt tccaaattcc ctccctttaa gcaccagctc gtttctgttc ttccacagcc
      661 tcccaagtat ccaagggact aaagcctcca cattcttcag atcaggatat tcttgtttaa
      721 gatgttgaac tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt
      781 cttcatagcg aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa
      841 tgaaaaaata ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa
      901 ataagatcca ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg
      961 ccttttttaa cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca
     1021 ataatcatac aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa
     1081 tatgttatac gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt
     1141 gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta
     1201 gaaaatacga aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca
     1261 gctccaccgt atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat
     1321 cgttaaatct caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt
     1381 cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc
     1441 aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg
     1501 tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc
     1561 tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa
     1621 agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc
     1681 aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga
     1741 ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt
     1801 agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt
     1861 ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg
     1921 atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga
     1981 ttaatctgag tttttctgat taacagaatg gctactaatc tttctagagt tgctggactt
     2041 gaaaagcaac ttgctattga acttaaggtt aagcaaggtg ctgaaaatat gattcaaact
     2101 tattctaatg gatctactaa ggatagaaag cttcttctta ctgctcaaca aatgcttcaa
     2161 gattctaaga ctaagattga tattattaga atgcaactta gaagagctct tcaagctggt
     2221 caacttgaaa atcaagctgc tcctggttcg atggtgagca agggcgagga gctgttcacc
     2281 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2341 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2401 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     2461 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2521 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2581 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2641 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2701 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2761 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2821 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2881 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2941 actctcggca tggacgagct gtacaagtga gcttaaatca ccagtctctc tctacaaatc
     3001 tatctctctc tataataatg tgtgagtagt tcccagataa gggaattagg gttcttatag
     3061 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     3121 acttctatca ataaaatttc taattcctaa aaccaaaatc cagcgctgtc atgagacgaa
     3181 ttctgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     3241 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     3301 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     3361 cggcttctga cgttcagtgc agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt
     3421 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     3481 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     3541 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     3601 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     3661 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     3721 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     3781 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     3841 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     3901 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     3961 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     4021 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     4081 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     4141 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     4201 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     4261 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg
     4321 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     4381 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     4441 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     4501 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     4561 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     4621 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     4681 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     4741 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     4801 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     4861 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     4921 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     4981 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     5041 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     5101 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     5161 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     5221 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     5281 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     5341 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     5401 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     5461 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     5521 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     5581 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     5641 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     5701 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     5761 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     5821 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     5881 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     5941 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     6001 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     6061 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     6121 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     6181 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     6241 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     6301 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     6361 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     6421 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     6481 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     6541 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     6601 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     6661 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta
     6721 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     6781 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     6841 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     6901 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     6961 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     7021 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     7081 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     7141 gacacatgca gctcccggtg acggtcacag cttgtctgta agcggatgcc gggagcagac
     7201 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     7261 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     7321 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     7381 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7441 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7501 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7561 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7621 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7681 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7741 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7801 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7861 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7921 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7981 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8041 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8101 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8161 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8221 gattttggtc atgcattcta ggtgattaga aaaactcatc gagcatcaaa tgaaactgca
     8281 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     8341 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     8401 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     8461 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     8521 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     8581 ccaaaccgtt attcattcgt gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa
     8641 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     8701 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga
     8761 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     8821 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     8881 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcggt
     8941 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     9001 catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga atatggctca
     9061 taacagaact tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
     9121 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
     9181 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
     9241 attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
     9301 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
     9361 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
     9421 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     9481 gttggctggc tggtggcagg atatattgtg gtgtaaaca
//
