LOCUS       sequence-422301-        7017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for an MLV Gag-mCherry fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pGag-mCherry
FEATURES             Location/Qualifiers
     source          1..7017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        82..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          799..1274
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1332..1748
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1678..1700
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1716..1732
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3666..3683)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3672..3879
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4023..4417
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(4749..4767)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4835..4939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4940..5008,5009..5800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5158..5177)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     6336..6355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct tacatgtggt accgagctcg
      781 gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc cccttctttt
      841 ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacacat attgaccaaa
      901 tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat atactttttt
      961 gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata atgatacaat
     1021 gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg gttaaggcaa
     1081 tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg taagaggttt
     1141 catattgcta atagcagcta caatccagct accattctgc ttttatttta tggttgggat
     1201 aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta
     1261 tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat cactttggca
     1321 aagaattcat gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1381 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1441 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1501 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1561 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1621 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1681 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1741 taggcgccaa acctaaacct caagttcttt ctgacagtgg ggggccgctc atcgacctac
     1801 ttacagaaga ccccccgcct tatagggacc caagaccacc cccttccgac agggacggaa
     1861 atggtggaga agcgacccct gcgggagagg caccggaccc ctccccaatg gcatctcgcc
     1921 tacgtgggag acgggagccc cctgtggccg actccactac ctcgcaggca ttccccctcc
     1981 gcgcaggagg aaacggacag cttcaatact ggccgttctc ctcttctgac ctttacaact
     2041 ggaaaaataa taacccttct ttttctgaag atccaggtaa actgacagct ctgatcgagt
     2101 ctgtcctcat cacccatcag cccacctggg acgactgtca gcagctgttg gggactctgc
     2161 tgaccggaga agaaaaacaa cgggtgctct tagaggctag aaaggcggtg cggggcgatg
     2221 atgggcgccc cactcaactg cccaatgaag tcgatgccgc ttttcccctc gagcgcccag
     2281 actgggatta caccacccag gcaggtagga accacctagt ccactatcgc cagttgctcc
     2341 tagcgggtct ccaaaacgcg ggcagaagcc ccaccaattt ggccaaggta aaaggaataa
     2401 cacaagggcc caatgagtct ccctcggcct tcctagagag acttaaggaa gcctatcgca
     2461 ggtacactcc ttatgaccct gaggacccag ggcaagaaac taatgtgtct atgtctttca
     2521 tttggcagtc tgccccagac attgggagaa agttagagag gttagaagat ttaaaaaaca
     2581 agacgcttgg agatttggtt agagaggcag aaaagatctt taataaacga gaaaccccgg
     2641 aagaaagaga ggaacgtatc aggagagaaa cagaggaaaa agaagaacgc cgtaggacag
     2701 aggatgagca gaaagagaaa gaaagagatc gtaggagaca tagagagatg agcaagctat
     2761 tggccactgt cgttagtgga cagaaacagg atagacaggg aggagaacga aggaggtccc
     2821 aactcgatcg cgaccagtgt gcctactgca aagaaaaggg gcactgggct aaagattgtc
     2881 ccaagaaacc acgaggacct cggggaccaa gaccgcaggg atcaggatca ggatcaggag
     2941 tgtccaaggg cgaggaagat aacatggcca tcatcaagga gttcatgagg tttaaggtcc
     3001 acatggaggg ttcagtcaat ggccacgagt tcgagattga aggcgagggc gagggccgcc
     3061 cctacgaagg gacacagacg gcgaaattga aggtgaccaa aggcgggcca ttgcccttcg
     3121 catgggacat cttgtcccct cagtttatgt atggcagcaa ggcctatgtt aagcaccccg
     3181 ctgatatccc ggactacttg aagctgtcct ttccagaggg gtttaaatgg gagcgcgtta
     3241 tgaatttcga agacggagga gtggttacgg tgacgcagga ctcatccctg caggacggag
     3301 aatttatata taaggttaag ttgagaggca caaacttccc aagcgacggc cctgtgatgc
     3361 agaagaaaac aatggggtgg gaagcttcca gcgagcgcat gtaccccgaa gatggcgccc
     3421 tcaagggcga gataaagcaa aggctgaaac ttaaggacgg cggtcattac gacgcggagg
     3481 tcaagacaac ttacaaggct aaaaaacccg ttcagttgcc tggggcttac aatgttaata
     3541 tcaaacttga catcacaagc cacaatgaag actatacgat cgtggagcag tatgaacgag
     3601 cggaaggcag gcactcaacg ggggggatgg acgagcttta caagtaacta gagctcgctg
     3661 atcagcctcg actgtgcctt ctagttacca gccatctgtt gtttgcccct cccccgtgcc
     3721 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3781 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3841 gggggaggat tgggaagaga atagcaggca tgctggggac atggcgcgtt caacgctctc
     3901 aaaacccctt aaaaataagg ttaacccgcg aggcccccta agaattcacc ccaccagtgc
     3961 aggctgccta tcagaaagtg gtggctggtg tggctaatgc cctggcccac aagtatcact
     4021 aagctcgctt tcttgctgtc caatttctat taaaggttcc tttgttccct aagtccaact
     4081 actaaactgg gggatattat gaagggcctt gagcatctgg attctgccta ataaaaaaca
     4141 tttattttca ttgcaatgat gtatttaaat tatttctgaa tattttacta aaaagggaat
     4201 gtgggaggtc agtgcattta aaacataaag aaatgaagag ctagttcaaa ccttgggaaa
     4261 atacactata tcttaaactc catgaaagaa ggtgaggctg caaacagcta atgcacattg
     4321 gcaacagccc ctgatgccta tgccttattc atccctcaga aaaggattca agtagaggct
     4381 tgatttggag gttaaagttt tgctatgctg tattttacat tacttattgt tttagctgtc
     4441 ctcatgaatg tcttttcact acccatttgc ttatcctgca tctctcagcc ttgactccac
     4501 tcagttctct tgcttagaga taccaccttt cccctgaagt gttccttcca tgttttacgg
     4561 cgagatggtt tctcctcgcc tggccactca gccttagttg tctctgttgt cttatagagg
     4621 tctacttgaa gaaggaaaaa cagggggcat ggtttgactg tcctgtgagc ccttcttccc
     4681 tgcctccccc actcacagtg acccggaatc cctcgacatg gcagtctaga attcttgaag
     4741 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4801 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4861 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4921 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4981 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5041 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5101 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5161 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     5221 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5281 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5341 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5401 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5461 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5521 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5581 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5641 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5701 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5761 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5821 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggctgctgc
     5881 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5941 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6001 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6061 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6121 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6181 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6241 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6301 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6361 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6421 cggagcctat ggaaaaacgc cagcaacgga gatgcgccgc gtgcggctgc tggagatggc
     6481 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
     6541 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
     6601 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
     6661 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
     6721 gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct
     6781 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     6841 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     6901 ggggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     6961 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatg
//
