LOCUS       sequence-422303-       10219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector to express MLV Gag with truncated MA domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pLV_CAG_Gag∆MA
FEATURES             Location/Qualifiers
     source          1..10219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(450..1241,1242..1310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1073..1092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1311..1415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1490..1509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1681..2060
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2062..2260
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2278..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2505..2630
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3123..3356
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3541..3585
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3883..4000
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4035..4413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4414..4617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4614..4638
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4751..5226
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             6244..6300
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6301..6996
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFAR
                     TFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAF
                     KTPDADAGEE"
     CDS             7006..7062
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7063..7434
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="AKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDV
                     TLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGRE
                     FALRDPAGNCVHFVAEEQD"
     misc_feature    7459..8047
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7512..7532)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7930..7941)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8050..8066)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8051..8067)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8572..8752
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8778..8795)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8784..9008
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9054..9482
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9141..9160)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9351..9372
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9508..9524)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9508..9524)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9521..9543)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9532..9548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9556..9562,9563..9580,9581..9586))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9601..9622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9739..9756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9910..10219,1..279))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9990..10009)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
       61 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1441 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1501 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1561 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1621 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     1681 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     1741 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     1801 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     1861 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     1921 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     1981 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2041 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2101 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2161 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2221 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     2281 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2341 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2401 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2461 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     2521 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     2581 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     2641 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     2701 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     2761 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     2821 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2881 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2941 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     3001 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     3061 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     3121 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     3181 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     3241 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     3301 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     3361 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     3421 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     3481 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     3541 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     3601 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     3661 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     3721 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     3781 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     3841 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     3901 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     3961 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4021 cagagatcca gtttacattg attattgact agttattaat agtaatcaat tacggggtca
     4081 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     4141 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     4201 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     4261 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     4321 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     4381 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     4441 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4501 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4561 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     4621 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     4681 caccgggacc gatccagcct cccctcgaag cttacatgtg gtaccgagct cggatcctga
     4741 gaacttcagg gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt
     4801 aagttcatgt cataggaagg ggagaagtaa cagggtacac atattgacca aatcagggta
     4861 attttgcatt tgtaatttta aaaaatgctt tcttctttta atatactttt ttgtttatct
     4921 tatttctaat actttcccta atctctttct ttcagggcaa taatgataca atgtatcatg
     4981 cctctttgca ccattctaaa gaataacagt gataatttct gggttaaggc aatagcaata
     5041 tttctgcata taaatatttc tgcatataaa ttgtaactga tgtaagaggt ttcatattgc
     5101 taatagcagc tacaatccag ctaccattct gcttttattt tatggttggg ataaggctgg
     5161 attattctga gtccaagcta ggcccttttg ctaatcatgt tcatacctct tatcttcctc
     5221 ccacagctcc tgggcaacgt gctggtctgt gtgctggccc atcactttgg caaagaattc
     5281 gccaccatgc ccctccgcgc aggaggaaac ggacagcttc aatactggcc gttctcctct
     5341 tctgaccttt acaactggaa aaataataac ccttcttttt ctgaagatcc aggtaaactg
     5401 acagctctga tcgagtctgt cctcatcacc catcagccca cctgggacga ctgtcagcag
     5461 ctgttgggga ctctgctgac cggagaagaa aaacaacggg tgctcttaga ggctagaaag
     5521 gcggtgcggg gcgatgatgg gcgccccact caactgccca atgaagtcga tgccgctttt
     5581 cccctcgagc gcccagactg ggattacacc acccaggcag gtaggaacca cctagtccac
     5641 tatcgccagt tgctcctagc gggtctccaa aacgcgggca gaagccccac caatttggcc
     5701 aaggtaaaag gaataacaca agggcccaat gagtctccct cggccttcct agagagactt
     5761 aaggaagcct atcgcaggta cactccttat gaccctgagg acccagggca agaaactaat
     5821 gtgtctatgt ctttcatttg gcagtctgcc ccagacattg ggagaaagtt agagaggtta
     5881 gaagatttaa aaaacaagac gcttggagat ttggttagag aggcagaaaa gatctttaat
     5941 aaacgagaaa ccccggaaga aagagaggaa cgtatcagga gagaaacaga ggaaaaagaa
     6001 gaacgccgta ggacagagga tgagcagaaa gagaaagaaa gagatcgtag gagacataga
     6061 gagatgagca agctattggc cactgtcgtt agtggacaga aacaggatag acagggagga
     6121 gaacgaagga ggtcccaact cgatcgcgac cagtgtgcct actgcaaaga aaaggggcac
     6181 tgggctaaag attgtcccaa gaaaccacga ggacctcggg gaccaagacc gcagggatcc
     6241 ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg
     6301 atggagagcg acgagagcgg cctgcccgcc atgaagatcg agtgccgcat caccggcacc
     6361 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccga gcagggccgc
     6421 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     6481 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     6541 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     6601 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     6661 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     6721 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     6781 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     6841 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     6901 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     6961 gccttcaaga ccccggatgc agatgccggt gaagaaggat ccggcgcaac aaacttctct
     7021 ctgctcaaac aagccggcga cgtcgaagag aatcctgggc cggccaagtt gaccagtgcc
     7081 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     7141 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     7201 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     7261 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     7321 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     7381 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgagaattc
     7441 gatatcaagc ttatcggtaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7501 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7561 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7621 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7681 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7741 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7801 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7861 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7921 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7981 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8041 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     8101 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     8161 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     8221 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     8281 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     8341 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     8401 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     8461 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     8521 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     8581 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8641 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8701 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     8761 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     8821 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     8881 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     8941 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     9001 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     9061 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     9121 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     9181 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     9241 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     9301 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     9361 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     9421 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     9481 ttaattctgt agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9661 cctgtcgtgt cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9721 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9781 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9841 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9901 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9961 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10021 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10081 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10141 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10201 atccggtaac tatcgtctt
//
