LOCUS       sequence-422309-       10534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector to express an MLV Gag-MCP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pLV_CAG_Gag-MCP
FEATURES             Location/Qualifiers
     source          1..10534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(36..140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(215..234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        406..785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        787..985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1003..1183
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1230..1355
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1848..2081
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2266..2310
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2608..2725
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2760..3138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3139..3342
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3339..3363
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3476..3951
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      4006..4015
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4015..4431
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4361..4383
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4399..4415
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             5650..6036
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             6046..6102
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6103..6474
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="AKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDV
                     TLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGRE
                     FALRDPAGNCVHFVAEEQD"
     misc_feature    6499..7087
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6552..6572)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6970..6981)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7090..7106)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7091..7107)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7612..7792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7818..7835)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7824..8048
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8094..8522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8181..8200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8391..8412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8548..8564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8548..8564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8561..8583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8572..8588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8596..8602,8603..8620,8621..8626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8641..8662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8779..8796)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8950..9538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9030..9049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9709..10500,10501..10534,1..35))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10332..10351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
       61 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      121 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
      181 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      241 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
      301 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
      361 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
      421 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      481 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      541 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      601 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      661 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      721 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      781 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      841 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      901 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      961 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
     1021 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1081 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1141 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     1201 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     1261 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     1321 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     1381 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     1441 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     1501 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1561 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1621 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1681 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1741 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1801 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1861 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1921 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1981 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2041 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2101 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     2161 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     2221 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     2281 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     2341 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2401 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2461 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2521 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     2581 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2641 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2701 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagttta
     2761 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2821 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2881 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2941 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3001 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3061 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3121 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3181 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3241 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3301 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     3361 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     3421 agcctcccct cgaagcttac atgtggtacc gagctcggat cctgagaact tcagggtgag
     3481 tctatgggac ccttgatgtt ttctttcccc ttcttttcta tggttaagtt catgtcatag
     3541 gaaggggaga agtaacaggg tacacatatt gaccaaatca gggtaatttt gcatttgtaa
     3601 ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt
     3661 ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt
     3721 ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat
     3781 atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa
     3841 tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca
     3901 agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gctcctgggc
     3961 aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag aattcgccac catgggccag
     4021 actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct
     4081 cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg
     4141 ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag
     4201 gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc
     4261 gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct
     4321 aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg
     4381 accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc caaacctaaa
     4441 cctcaagttc tttctgacag tggggggccg ctcatcgacc tacttacaga agaccccccg
     4501 ccttataggg acccaagacc acccccttcc gacagggacg gaaatggtgg agaagcgacc
     4561 cctgcgggag aggcaccgga cccctcccca atggcatctc gcctacgtgg gagacgggag
     4621 ccccctgtgg ccgactccac tacctcgcag gcattccccc tccgcgcagg aggaaacgga
     4681 cagcttcaat actggccgtt ctcctcttct gacctttaca actggaaaaa taataaccct
     4741 tctttttctg aagatccagg taaactgaca gctctgatcg agtctgtcct catcacccat
     4801 cagcccacct gggacgactg tcagcagctg ttggggactc tgctgaccgg agaagaaaaa
     4861 caacgggtgc tcttagaggc tagaaaggcg gtgcggggcg atgatgggcg ccccactcaa
     4921 ctgcccaatg aagtcgatgc cgcttttccc ctcgagcgcc cagactggga ttacaccacc
     4981 caggcaggta ggaaccacct agtccactat cgccagttgc tcctagcggg tctccaaaac
     5041 gcgggcagaa gccccaccaa tttggccaag gtaaaaggaa taacacaagg gcccaatgag
     5101 tctccctcgg ccttcctaga gagacttaag gaagcctatc gcaggtacac tccttatgac
     5161 cctgaggacc cagggcaaga aactaatgtg tctatgtctt tcatttggca gtctgcccca
     5221 gacattggga gaaagttaga gaggttagaa gatttaaaaa acaagacgct tggagatttg
     5281 gttagagagg cagaaaagat ctttaataaa cgagaaaccc cggaagaaag agaggaacgt
     5341 atcaggagag aaacagagga aaaagaagaa cgccgtagga cagaggatga gcagaaagag
     5401 aaagaaagag atcgtaggag acatagagag atgagcaagc tattggccac tgtcgttagt
     5461 ggacagaaac aggatagaca gggaggagaa cgaaggaggt cccaactcga tcgcgaccag
     5521 tgtgcctact gcaaagaaaa ggggcactgg gctaaagatt gtcccaagaa accacgagga
     5581 cctcggggac caagaccgca gggtggcggt ggctcgggcg gtggtgggtc gggtggcggc
     5641 ggatctatgg cttcaaactt tactcagttc gtgctcgtgg acaatggtgg gacaggggat
     5701 gtgacagtgg ctccttctaa tttcgctaat ggggtggcag agtggatcag ctccaactca
     5761 cggagccagg cctacaaggt gacatgcagc gtcaggcagt ctagtgccca gaagagaaag
     5821 tataccatca aggtggaggt ccccaaagtg gctacccaga cagtgggcgg agtcgaactg
     5881 cctgtcgccg cttggaggtc ctacctgaac atggagctca ctatcccaat tttcgctacc
     5941 aattctgact gtgaactcat cgtgaaggca atgcaggggc tcctcaaaga cggtaatcct
     6001 atcccttccg ccatcgccgc taactcaggt atctacggat ccggcgcaac aaacttctct
     6061 ctgctcaaac aagccggcga cgtcgaagag aatcctgggc cggccaagtt gaccagtgcc
     6121 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     6181 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     6241 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     6301 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     6361 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     6421 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgagaattc
     6481 gatatcaagc ttatcggtaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6541 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6601 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6661 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6721 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6781 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6841 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6901 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6961 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7021 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7081 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     7141 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     7201 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     7261 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7321 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     7381 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     7441 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     7501 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     7561 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     7621 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7681 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7741 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     7801 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7861 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     7921 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     7981 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     8041 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     8101 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8161 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8221 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8281 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8341 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8401 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8461 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8521 ttaattctgt agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8581 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8641 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8701 cctgtcgtgt cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8761 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8821 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8881 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8941 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9361 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9481 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9601 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9661 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9721 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9781 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9841 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9901 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9961 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10021 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10081 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10141 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10201 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10261 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10321 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10381 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10441 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10501 agcaaaaaca ggaaggcaaa atgccgcaaa aaag
//
