LOCUS       sequence-422310-       10510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector to express His-VSVG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pLV_CMV_His-VSVG
FEATURES             Location/Qualifiers
     source          1..10510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(450..1241,1242..1310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1073..1092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1311..1415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1490..1509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1681..2060
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2062..2260
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2278..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2505..2630
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3123..3356
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3541..3585
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3883..4000
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4035..4413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4414..4617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4662..4681
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4662..4680
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4783..4800
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6247..6279
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6295..6351
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7069..7125
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7126..7725
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7126..7145)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7622..7642
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7750..8338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7803..7823)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8221..8232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8341..8357)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8342..8358)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8863..9043
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9069..9086)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9075..9299
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9345..9773
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9432..9451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9642..9663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9799..9815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9799..9815)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9812..9834)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9823..9839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9847..9853,9854..9871,9872..9877))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9892..9913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10030..10047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10201..10510,1..279))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10281..10300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
       61 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1441 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1501 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1561 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1621 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     1681 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     1741 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     1801 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     1861 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     1921 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     1981 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2041 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2101 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2161 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2221 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     2281 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2341 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2401 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2461 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     2521 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     2581 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     2641 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     2701 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     2761 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     2821 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2881 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2941 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     3001 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     3061 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     3121 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     3181 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     3241 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     3301 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     3361 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     3421 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     3481 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     3541 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     3601 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     3661 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     3721 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     3781 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     3841 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     3901 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     3961 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4021 cagagatcca gtttacattg attattgact agttattaat agtaatcaat tacggggtca
     4081 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     4141 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     4201 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     4261 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     4321 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     4381 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     4441 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4501 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4561 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     4621 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
     4681 gagacccaag ctggctagcc caccatgaag tgccttttgt acttagcctt tttattcatt
     4741 ggggtgaatt gcaagttcac catagttttt ccatccggag gacatcatca ccatcaccat
     4801 ggcggaagct tgggacacaa ccaaaaagga aactggaaaa atgttccttc taattaccat
     4861 tattgcccgt caagctcaga tttaaattgg cataatgact taataggcac agccttacaa
     4921 gtcaaaatgc ccaagagtca caaggctatt caagcagatg gttggatgtg tcatgcttcc
     4981 aaatgggtca ctacttgtga tttccgctgg tatggaccga agtatataac acattccatc
     5041 cgatccttca ctccatctgt agaacaatgc aaggaaagca ttgaacaaac gaaacaagga
     5101 acttggctga atccaggctt ccctcctcaa agttgtggat atgcaactgt gacggatgcc
     5161 gaagcagtga ttgtccaggt gactcctcac catgtgctgg ttgatgaata cacaggagaa
     5221 tgggttgatt cacagttcat caacggaaaa tgcagcaatt acatatgccc cactgtccat
     5281 aactctacaa cctggcattc tgactataag gtcaaagggc tatgtgattc taacctcatt
     5341 tccatggaca tcaccttctt ctcagaggac ggagagctat catccctggg aaaggagggc
     5401 acagggttca gaagtaacta ctttgcttat gaaactggag gcaaggcctg caaaatgcaa
     5461 tactgcaagc attggggagt cagactccca tcaggtgtct ggttcgagat ggctgataag
     5521 gatctctttg ctgcagccag attccctgaa tgcccagaag ggtcaagtat ctctgctcca
     5581 tctcagacct cagtggatgt aagtctaatt caggacgttg agaggatctt ggattattcc
     5641 ctctgccaag aaacctggag caaaatcaga gcgggtcttc caatctctcc agtggatctc
     5701 agctatcttg ctcctaaaaa cccaggaacc ggtcctgctt tcaccataat caatggtacc
     5761 ctaaaatact ttgagaccag atacatcaga gtcgatattg ctgctccaat cctctcaaga
     5821 atggtcggaa tgatcagtgg aactaccaca gaaagggaac tgtgggatga ctgggcacca
     5881 tatgaagacg tggaaattgg acccaatgga gttctgagga ccagttcagg atataagttt
     5941 cctttataca tgattggaca tggtatgttg gactccgatc ttcatcttag ctcaaaggct
     6001 caggtgttcg aacatcctca cattcaagac gctgcttcgc aacttcctga tgatgagagt
     6061 ttattttttg gtgatactgg gctatccaaa aatccaatcg agcttgtaga aggttggttc
     6121 agtagttgga aaagctctat tgcctctttt ttctttatca tagggttaat cattggacta
     6181 ttcttggttc tccgagttgg tatccatctt tgcattaaat taaagcacac caagaaaaga
     6241 cagatttata cagacataga gatgaaccga cttggaaagg aattcggatc cggcgcaaca
     6301 aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc gatggtgtcc
     6361 aagggcgagg aagataacat ggccatcatc aaggagttca tgaggtttaa ggtccacatg
     6421 gagggttcag tcaatggcca cgagttcgag attgaaggcg agggcgaggg ccgcccctac
     6481 gaagggacac agacggcgaa attgaaggtg accaaaggcg ggccattgcc cttcgcatgg
     6541 gacatcttgt cccctcagtt tatgtatggc agcaaggcct atgttaagca ccccgctgat
     6601 atcccggact acttgaagct gtcctttcca gaggggttta aatgggagcg cgttatgaat
     6661 ttcgaagacg gaggagtggt tacggtgacg caggactcat ccctgcagga cggagaattt
     6721 atatataagg ttaagttgag aggcacaaac ttcccaagcg acggccctgt gatgcagaag
     6781 aaaacaatgg ggtgggaagc ttccagcgag cgcatgtacc ccgaagatgg cgccctcaag
     6841 ggcgagataa agcaaaggct gaaacttaag gacggcggtc attacgacgc ggaggtcaag
     6901 acaacttaca aggctaaaaa acccgttcag ttgcctgggg cttacaatgt taatatcaaa
     6961 cttgacatca caagccacaa tgaagactat acgatcgtgg agcagtatga acgagcggaa
     7021 ggcaggcact caacgggggg gatggacgag ctttacaagg gatccggcgc aacaaacttc
     7081 tctctgctca aacaagccgg cgacgtcgaa gagaatcctg ggccgatgac cgagtacaag
     7141 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     7201 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     7261 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7321 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7381 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7441 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7501 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7561 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7621 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7681 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctaagaatt cgatatcaag
     7741 cttatcggta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7801 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7861 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7921 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7981 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     8041 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     8101 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     8161 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8221 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8281 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     8341 cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     8401 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     8461 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     8521 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     8581 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     8641 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     8701 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     8761 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     8821 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     8881 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8941 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9001 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     9061 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     9121 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     9181 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     9241 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     9301 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     9361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9601 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9661 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     9721 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     9781 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9841 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9901 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9961 tcagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10501 ctatcgtctt
//
