LOCUS       sequence-422313-       10404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac vector to express FLAG-VSVG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pPB_EF1a_FLAG-VSVG
FEATURES             Location/Qualifiers
     source          1..10404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   127..189
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1356..2534
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1587..2525
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2482..2502
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2625..2648
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4095..4127
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             4143..4199
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4917..4973
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4974..5573
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4974..4993)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5470..5490
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5589..6177
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5642..5662)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6060..6071)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6227..6348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6264..6283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6318..6337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7111..7145
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7636..7652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7636..7652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7649..7671)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7660..7676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7684..7690,7691..7708,7709..7714))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7729..7750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7867..7884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8038..8626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8118..8137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8797..9588,9589..9657))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9420..9439
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9658..9762)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9788..10243)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9925..9946)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10137..10156
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10370..10392
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10384..10401
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10385..10401
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat
       61 gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagcta
      121 gattaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgtgtaaaat
      181 tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt
      241 aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg
      301 tttatttatt tattaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt
      361 ttatgaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
      421 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
      481 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
      541 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaggcc
      601 tccaccataa agatggttaa ttaacccacc caagatctgg atcgataagc ttgatatcga
      661 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
      721 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
      781 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
      841 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
      901 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
      961 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     1021 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     1081 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     1141 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg ccccgcgcgc
     1201 agctcccggc tccctccccc ttcggatgtg gcttgagctg taggcgcgga gtcctgcagc
     1261 cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     1321 ctgtaggttt ggcaatcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     1381 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg
     1441 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     1501 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     1561 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     1621 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     1681 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     1741 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     1801 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     1861 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     1921 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     1981 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     2041 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     2101 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     2161 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     2221 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     2281 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     2341 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     2401 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     2461 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     2521 ttcaggtgtc gtgacgtacg gccaccatga agtgcctttt gtacttagcc tttttattca
     2581 ttggggtgaa ttgcaagttc accatagttt ttccatccgg aggagattac aaggatgacg
     2641 acgataaggg cggaagcttg ggacacaacc aaaaaggaaa ctggaaaaat gttccttcta
     2701 attaccatta ttgcccgtca agctcagatt taaattggca taatgactta ataggcacag
     2761 ccttacaagt caaaatgccc aagagtcaca aggctattca agcagacggt tggatgtgtc
     2821 atgcttccaa atgggtcact acttgtgatt tccgctggta tggaccgaag tatataacac
     2881 attccatccg atccttcact ccatctgtag aacaatgcaa ggaaagcatt gaacaaacga
     2941 aacaaggaac ttggctgaat ccaggcttcc ctcctcaaag ttgtggatat gcaactgtga
     3001 cggatgccga agcagtgatt gtccaggtga ctcctcacca tgtgctggtt gatgaataca
     3061 caggagaatg ggttgattca cagttcatca acggaaaatg cagcaattac atatgcccca
     3121 ctgtccataa ctctacaacc tggcattctg actataaggt caaagggcta tgtgattcta
     3181 acctcatttc catggacatc accttcttct cagaggacgg agagctatca tccctgggaa
     3241 aggagggcac agggttcaga agtaactact ttgcttatga aactggaggc aaggcctgca
     3301 aaatgcaata ctgcaagcat tggggagtca gactcccatc aggtgtctgg ttcgagatgg
     3361 ctgataagga tctctttgct gcagccagat tccctgaatg cccagaaggg tcaagtatct
     3421 ctgctccatc tcagacctca gtggatgtaa gtctaattca ggacgttgag aggatcttgg
     3481 attattccct ctgccaagaa acctggagca aaatcagagc gggtcttcca atctctccag
     3541 tggatctcag ctatcttgct cctaaaaacc caggaaccgg tcctgctttc accataatca
     3601 atggtaccct aaaatacttt gagaccagat acatcagagt cgatattgct gctccaatcc
     3661 tctcaagaat ggtcggaatg atcagtggaa ctaccacaga aagggaactg tgggatgact
     3721 gggcaccata tgaagacgtg gaaattggac ccaatggagt tctgaggacc agttcaggat
     3781 ataagtttcc tttatacatg attggacatg gtatgttgga ctccgatctt catcttagct
     3841 caaaggctca ggtgttcgaa catcctcaca ttcaagacgc tgcttcgcaa cttcctgatg
     3901 atgagagttt attttttggt gatactgggc tatccaaaaa tccaatcgag cttgtagaag
     3961 gttggttcag tagttggaaa agctctattg cctctttttt ctttatcata gggttaatca
     4021 ttggactatt cttggttctc cgagttggta tccatctttg cattaaatta aagcacacca
     4081 agaaaagaca gatttataca gacatagaga tgaaccgact tggaaaggaa ttcggatccg
     4141 gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga
     4201 tggtgtccaa gggcgaggaa gataacatgg ccatcatcaa ggagttcatg aggtttaagg
     4261 tccacatgga gggttcagtc aatggccacg agttcgagat tgaaggcgag ggcgagggcc
     4321 gcccctacga agggacacag acggcgaaat tgaaggtgac caaaggcggg ccattgccct
     4381 tcgcatggga catcttgtcc cctcagttta tgtatggcag caaggcctat gttaagcacc
     4441 ccgctgatat cccggactac ttgaagctgt cctttccaga ggggtttaaa tgggagcgcg
     4501 ttatgaattt cgaagacgga ggagtggtta cggtgacgca ggactcatcc ctgcaggacg
     4561 gagaatttat atataaggtt aagttgagag gcacaaactt cccaagcgac ggccctgtga
     4621 tgcagaagaa aacaatgggg tgggaagctt ccagcgagcg catgtacccc gaagatggcg
     4681 ccctcaaggg cgagataaag caaaggctga aacttaagga cggcggtcat tacgacgcgg
     4741 aggtcaagac aacttacaag gctaaaaaac ccgttcagtt gcctggggct tacaatgtta
     4801 atatcaaact tgacatcaca agccacaatg aagactatac gatcgtggag cagtatgaac
     4861 gagcggaagg caggcactca acggggggga tggacgagct ttacaaggga tccggcgcaa
     4921 caaacttctc tctgctcaaa caagccggcg acgtcgaaga gaatcctggg ccgatgaccg
     4981 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     5041 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     5101 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     5161 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     5221 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     5281 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     5341 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     5401 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     5461 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     5521 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaacgcgtt
     5581 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5641 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5701 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5761 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5821 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5881 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5941 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6001 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6061 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6121 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctc
     6181 gagtctagag ggcccgttta aacccgctga tcagcctaac taactaaact tgtttattgc
     6241 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6301 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggaa
     6361 ttgactcaaa tgatgtcaat tagtctatca gaagctcatc tggtctccct tccgggggac
     6421 aagacatccc tgtttaatat ttaaacagca gtgttcccaa actgggttct tatatccctt
     6481 gctctggtca accaggttgc agggtttcct gtcctcacag gaacgaagtc cctaaagaaa
     6541 cagtggcagc caggtttagc cccggaattg actggattcc ttttttaggg cccattggta
     6601 tggctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc
     6661 agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc
     6721 tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc
     6781 ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct gtccctgata
     6841 tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa
     6901 tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt
     6961 gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct
     7021 cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
     7081 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
     7141 tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc
     7201 gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg
     7261 ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa
     7321 aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac
     7381 tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga
     7441 tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg
     7501 ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt
     7561 atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg
     7621 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7681 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7741 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7801 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7861 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7921 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7981 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8041 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8101 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8161 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8221 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8281 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8341 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8401 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8461 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8521 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8581 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8641 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8701 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8761 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8821 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8881 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8941 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9001 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9061 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9121 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9181 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9241 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9301 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9361 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9421 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9481 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9541 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9601 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9661 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9721 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9781 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     9841 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     9901 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     9961 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
    10021 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
    10081 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
    10141 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
    10201 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
    10261 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    10321 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    10381 cgttgtaaaa cgacggccag tgag
//
