LOCUS       sequence-422346-        9978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR - 
            PuroR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Wilkins KC, Schroeder T, Gu S, Revalde JL, Floor SN
  TITLE     A novel reporter for helicase activity in translation uncovers DDX3X
            interactions.
  JOURNAL   RNA. 2024 Jul 16;30(8):1041-1057. doi: 10.1261/rna.079837.123.
  PUBMED    38697667
REFERENCE   2  (bases 1 to 9978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.079837"; journalName: "RNA"; date: "2024-07-16- 16"; 
            volume: "30"; issue: "8"; pages: "1041-1057"
COMMENT     Sequence Label: KW63_HART_RAC1_PuroR
FEATURES             Location/Qualifiers
     source          1..9978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1135)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1785..1804
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1785..1803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      1824..1959
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1999..2120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             2192..2425
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    2512..3100
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2618..2629
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3027..3047
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(3524..3543)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3793..3811
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        4609..4647
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4665..4674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4671..4673,4674..4676,4677..5384)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(4671..4673,4674..4676,4677..5384)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(4716..4737)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4977..4996)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5324..5345
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5403..5456
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5469..6068
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5469..5488)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5965..5985
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6288..6422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6559..6676
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6943..6987)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7172..7405
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7898..8023
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8070..8250
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8265..8468)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        8469..8848
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(8949..8969)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8949..8967)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8988..9004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8988..9004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9001..9023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9012..9028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9036..9042,9043..9060,9061..9066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9081..9102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9219..9236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9390..9978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9470..9489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcacaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtacgtcc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     1861 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     1921 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat
     1981 agctagagcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     2041 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2101 taagctgcaa taaacaagtt cctctcactc tctgatattc atttctttgc aagttataaa
     2161 tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag
     2221 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     2281 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     2341 caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt
     2401 cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct
     2461 acagctgcct tgtaagtcat tggtcttaaa ggtaccgagc tcgaattcca ggcggggagg
     2521 cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca
     2581 gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc
     2641 agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccat ggaaaggacg
     2701 tcagcttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc
     2761 agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag
     2821 tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa
     2881 acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca
     2941 gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat
     3001 acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac
     3061 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gtcgactcta gactcgactc
     3121 tacacattga tcctagcaga agcacaggct gcagggtgac ggtccatccc gctctcctgg
     3181 gcacaagaca tgggcagcgc gccatcatcc tgctcctcca cctccggcgg gaagccatgg
     3241 ctaagcttct tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg
     3301 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt gatgttgacg
     3361 ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcagcg
     3421 tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt cagggcgccg
     3481 tcctcggggt acattctctc ggaggaggcc tcccagccca tggtcttctt ctgcattacg
     3541 gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc
     3601 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     3661 tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc
     3721 ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca ggcgaagggc
     3781 agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc
     3841 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
     3901 aagcgcatga actccttgat gatggccatg ttatcttctt caccttttga aaccatcagg
     3961 gccgctcgct gggccgcggc ggcggcggca gggcgctgag ataggaagcg gcgggctcgg
     4021 ggcgcggcgg acgggcgcgc ggcttgcggg cggcggggcg ctgggtgccg gggccgctgt
     4081 ccacgccgcc tcgccctccg cggactggaa gccgatgccc ggcagcggcc accacccaaa
     4141 gctgaggaaa actgcgcggg caggaacagg gcccacacta ccgccccaca ccccgcctcc
     4201 cgcaccgccc cttcccggcc gctgctctcg gcgcgccctg ctgagcagcc gctattggcc
     4261 acagcccatc gcggtcggcg cgctgccatt gctccctggc gctgtccgtc tgcgagggta
     4321 ctagtgagac gtgcggcttc cgtttgtcac gtccggcacg ccgcgaaccg caaggaacct
     4381 tcccgactta ggggcggagc aggaagcgtc gccggggggc ccacaagggt agcggcgaag
     4441 atccgggtga cgctgcgaac ggacgtgaag aatgtgcgag acccagggtc ggcgccgctg
     4501 cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg gctgccttgg
     4561 aaaaggcgca accccaaccc cgtggaattc gatatcaagc ttctcgaggg taggcgtgta
     4621 cggtgggagg cctatataag cagagctcgt ttagtgaacc gctagccacc atggtgagca
     4681 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4741 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4801 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4861 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4921 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4981 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5041 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5101 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5161 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5221 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5281 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5341 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacactgct agcggatcgg
     5401 gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccaccgg
     5461 tcgccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     5521 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5581 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5641 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5701 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5761 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5821 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5881 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5941 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6001 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6061 gtgcctagat gactaaacgc gtgctacgtt aattaaaccg gaccggtgga tccagaccac
     6121 ctcccctgcg agctaagctg gacagccaat gacgggtaag agagtgacat ttttcactaa
     6181 cctaagacag gagggccgtc agagctactg cctaatccaa agacgggtaa aagtgataaa
     6241 aatgtatcac tccaacctaa gacaggcgca gcttccgagg gatttgagat ccagacatga
     6301 taagatacat tgatgagttt ggacaaacca aaactagaat gcagtgaaaa aaatgcctta
     6361 tttgtgaaat ttgtgatgct attgccttat ttgtaaccat tataagctgc aataaacaag
     6421 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     6481 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag atctctcgag
     6541 gctagtctcg tgatcgataa aattttgaat ttttgtaatt tgtttttgta attctttagt
     6601 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
     6661 cccttttctt ttaaaagtta agcgataccg tcgagatccg ttcactaatc gaatggatct
     6721 gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg
     6781 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
     6841 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa
     6901 ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc
     6961 taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga
     7021 gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt
     7081 ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag cagtggtgca
     7141 aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc
     7201 cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg
     7261 ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa
     7321 ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc
     7381 caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct
     7441 tctctttgcc ttggtgggta ctactcctaa tggttcaatt tttactactt tatatttata
     7501 taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg
     7561 cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct
     7621 tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata
     7681 atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc
     7741 tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc
     7801 ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt
     7861 tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct
     7921 ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc
     7981 ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt
     8041 tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg
     8101 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     8161 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     8221 ggctcagatc tggtctaacc agagagaccc cggttcacta aacgagctct gcttatatag
     8281 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     8341 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     8401 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     8461 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     8521 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     8581 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     8641 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     8701 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     8761 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     8821 tattaataac tagtcaataa tcaatgtcaa catggcggta atgttggaca tgagccaata
     8881 taaatgtaca tattatgata tggatacaac gtatgcaatg gccaagcttg cagctccagc
     8941 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     9001 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9061 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9121 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9181 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9241 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9301 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9361 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9421 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9481 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9541 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9601 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9661 cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9721 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9781 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9841 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9901 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9961 agaaaaaaag gatctcaa
//
