LOCUS       sequence-422349-        8160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Calprotectin sensing promoter with luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Xia JY, Hepler C, Tran P, Waldeck NJ, Bass J, Prindle A
  TITLE     Engineered calprotectin-sensing probiotics for IBD surveillance in 
            humans.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2221121120. doi: 
            10.1073/pnas.2221121120. Epub 2023 Jul 31.
  PUBMED    37523538
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-8"; volume: "120(32):e2221121120. 
            doi"; pages: " 10.1073/pnas.2221121120. Epub 2023 Jul 31"
COMMENT     Sequence Label: ykgMO-IGS-LuxCDABE
FEATURES             Location/Qualifiers
     source          1..8160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             683..2125
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             2138..3061
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVALRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             4245..4904
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4311..4330)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      4925..5019
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(5426..5443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5560..6105)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5640..5659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6317..6351
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     RBS             6721..6732
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6739..7821
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGVTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             join(7836..8160,1..659)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAAADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMKYT"
ORIGIN
        1 aaaaaaaaga tgaaatgcat ttttttaatc gcccggttga atatcaacag caactatttg
       61 aagagtgtta tgaaatcatt aacgatgctt taacaacagg ctattgtaat ccagataacg
      121 atttttatag cttccctaaa atatctgtaa atccccatgc ttatacgcca ggcggacctc
      181 ggaaatatgt aacagcaacc agtcatcata ttgttgagtg ggcggccaaa aaaggtattc
      241 ctctcatctt taagtgggat gattctaatg atgttagata tgaatatgct gaaagatata
      301 aagccgctgc ggataaatat gacgttgacc tatcagagat agaccatcag ttaatgatat
      361 tagttaacta taacgaagat agtaataaag ctaaacaaga gacgcgtgca tttattagtg
      421 attatgttct tgaaatgcac cctaatgaaa atttcgaaaa taaacttgaa gaaataattg
      481 cagaaaacgc tgtcggaaat tatacggagt gtataactgc ggctaagttg gcaattgaaa
      541 agtgtggtgc gaaaagtgta ttgctgtcct ttgaaccaat gaatgatttg atgagccaaa
      601 aaaatgtaat caatattgtt gatgataata ttaagaagta ccacatgaaa tatacctaat
      661 aggtaccagg aggaaggcaa atatgactaa aaaaatttca ttcattatta acggccaggt
      721 tgaaatcttt cccgaaggtg atgatttagt gcaatccatt aattttggtg ataatagtgt
      781 ttacctgcca atattgaatg actctcatgt aaaaaacatt attgattgta atggaaataa
      841 cgaattacgg ttgcataaca ttgtcaattt tctctatacg gtagggcaaa gatggaaaaa
      901 tgaagaatac tcaagacgca ggacatacat tcgtgactta aaaaaatata tgggatattc
      961 agaagaaatg gctaagctag aggccaattg gatatctatg attttatgtt ctaaaggcgg
     1021 cctttatgat gttgtagaaa atgaacttgg ttctcgccat atcatggatg aatggctacc
     1081 tcaggatgaa agttatgttc gggcttttcc gaaaggtaaa tctgtacatc tgttggcagg
     1141 taatgttcca ttatctggga tcatgtctat attacgcgca attttaacta agaatcagtg
     1201 tattataaaa acatcgtcaa ccgatccttt taccgctaat gcattagcgt taagttttat
     1261 tgatgtagac cctaatcatc cgataacgcg ctctttatct gttatatatt ggccccacca
     1321 aggtgataca tcactcgcaa aagaaattat gcaacatgcg gatgttattg tcgcttgggg
     1381 agggccagat gcgattaatt gggcggtaga gcatgcgcca tcttatgctg atgtgattaa
     1441 atttggttct aaaaagagtc tttgcattat cgataatcct gttgatttga cgtccgcagc
     1501 gacaggtgcg gctcatgatg tttgttttta cgatcagcga gcttgttttt ctgcccaaaa
     1561 catatattac atgggaaatc attatgagga atttaagtta gcgttgatag aaaaacttaa
     1621 tctatatgcg catatattac cgaatgccaa aaaagatttt gatgaaaagg cggcctattc
     1681 tttagttcaa aaagaaagct tgtttgctgg attaaaagta gaggtggata ttcatcaacg
     1741 ttggatgatt attgagtcaa atgcaggtgt ggaatttaat caaccacttg gcagatgtgt
     1801 gtaccttcat cacgtcgata atattgagca aatattgcct tatgttcaaa aaaataagac
     1861 gcaaaccata tctatttttc cttgggagtc atcattcaaa tatcgagatg cgttagcatt
     1921 aaaaggtgcg gaaaggattg tagaagcagg aatgaataac atatttcgag ttggtggatc
     1981 tcatgacgga atgagaccgt tgcaacgatt agtgacatat atttctcatg aaaggccatc
     2041 taactatacg gctaaggatg ttgcggttga aatagaacag actcgattcc tggaagaaga
     2101 taagttcctt gtatttgtcc cataataggt aaaaagtatg gaaaatgaat caaaatataa
     2161 aaccatcgac cacgttattt gtgttgaagg aaataagaaa attcatgttt gggaaacgct
     2221 gccagaagaa aacagcccaa agagaaagaa tgccattatt attgcgtctg gttttgcccg
     2281 caggatggat cattttgctg gtctggcgga atatttatcg cggaatggat ttcatgtgat
     2341 ccgctatgat tcgcttcacc acgttggatt gagttcaggg acaattgatg aatttacaat
     2401 gtctatagga aagcagagct tgttagcagt ggttgattgg ttaactacac gaaaaataaa
     2461 taacttcggt atgttggctt caagcttatc tgcgcggata gcttatgcaa gcctatctga
     2521 aatcaatgct tcgtttttaa tcaccgcagt cggtgttgtt aacttaagat attctcttga
     2581 aagagcttta gggtttgatt atctcagtct acccattaat gaattgccga ataatctaga
     2641 ttttgaaggc cataaattgg gtgctgaagt ctttgcgaga gattgtcttg attttggttg
     2701 ggaagattta gcttctacaa ttaataacat gatgtatctt gatataccgt ttattgcttt
     2761 tactgcaaat aacgataatt gggtcaagca agatgaagtt atcacattgt tatcaaatat
     2821 tcgtagtaat cgatgcaaga tatattcttt gttaggaagt tcgcatgact tgagtgaaaa
     2881 tttagtggcc ctgcgcaatt tttatcaatc ggttacgaaa gccgctatcg cgatggataa
     2941 tgatcatctg gatattgatg ttgatattac tgaaccgtca tttgaacatt taactattgc
     3001 gacagtcaat gaacgccgaa tgagaattga gattgaaaat caagcaattt ctctgtctta
     3061 aaggatcctg aggaggaaaa caggtatgac ttcatatgtt gataaacaag aaattacagc
     3121 aagctcagaa attgatgatt tgattttttc gagcgatcca ttagtgtggt cttacgacga
     3181 gcaggaaaaa atcagaaaga aacttgtgct tgatgcattt cgtaatcatt ataaacattg
     3241 tcgagaatat cgtcactact gtcaggcaca caaagtagat gacaatatta cggaaattga
     3301 tgacatacct gtattcccaa catcggtttt taagtttact cgcttattaa cttctcagga
     3361 aaacgagatt gaaagttggt ttaccagtag cggcacgaat ggtttaaaaa gtcaggtggc
     3421 gcgtgacaga ttaagtattg agagactctt aggctctgtg agttatggca tgaaatatgt
     3481 tggtagttgg tttgatcatc aaatagaatt agtcaatttg ggaccagata gatttaatgc
     3541 tcataatatt tggtttaaat atgttatgag tttggtggaa ttgttatatc ctacgacatt
     3601 taccgtaaca gaagaacgaa tagattttgt taaaacattg aatagtcttg aacgaataaa
     3661 aaatcaaggg aaagatcttt gtcttattgg ttcgccatac tttatttatt tactctgcca
     3721 ttatatgaaa gataaaaaaa tctcattttc tggagataaa agcctttata tcataaccgg
     3781 aggcggctgg aaaagttacg aaaaagaatc tctgaaacgt gatgatttca atcatctttt
     3841 atttgatact ttcaatctca gtgatattag tcagatccga gatatattta atcaagctga
     3901 actcaacact tgtttctttg aggatgaaat gcagcgtaaa catgttccgc cgtgggtata
     3961 tgcgcgagcg cttgatcctg aaacgttgaa acctgtacct gatggaacgc cggggttgat
     4021 gagttatatg gatgcgtcag caaccagtta tccagcattt attgttaccg atgatgtcgg
     4081 gataattagc agctgttgtt tgtcggtgaa ctggatcctt actcgagtct agactgcagt
     4141 tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     4201 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     4261 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     4321 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     4381 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     4441 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     4501 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     4561 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     4621 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     4681 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     4741 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     4801 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     4861 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg
     4921 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     4981 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagcctccg atcaacgtct
     5041 cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt
     5101 tattatgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat
     5161 gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctat
     5221 ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
     5281 cgccgtacat cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt
     5341 gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata
     5401 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     5461 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga
     5521 tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc
     5581 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     5641 aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg
     5701 gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca
     5761 gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     5821 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag
     5881 caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc
     5941 ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct
     6001 cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt
     6061 tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt
     6121 aatcagataa aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
     6181 gtcagcccca tacgatataa gttgtaattc tcatgtttga cagcttatca tcgataagct
     6241 tccgatggcg cgccgagagg ctttacactt tatccttagc tttcgctaag gatgatttct
     6301 ggaattctaa agatctttta cggctagctc agccctaggt attatgctag ctactataac
     6361 ggcaataaac tgttcacttc agtgatattt aaaatatgca tcctctccct tttttgtaag
     6421 taattattat atccgtggga gaggaataca cattgtcagg taatcaatca tgctgcaata
     6481 aatcatcggc cagtaaagtg gagatagcct ccattctcga aaaatccata ctctcagcga
     6541 aaccatcatc aatcactcat ccaggcgttt atgggagcgt cgccaatggc tgctaacaat
     6601 gccagacttc cccgttgcgg aaattccaca tcccacaaat agtcacagtg attgggtgtt
     6661 gaaatgatcc ggatgagcat gtatctttac ggttatgtta taacataaca ggtaaaaatg
     6721 aaagaggaga aaggatctat gaaatttggc aactttttgc ttacatacca acctccccaa
     6781 ttttctcaaa cagaggtaat gaaacgtttg gttaaattag gtcgcatctc tgaggagtgt
     6841 ggttttgata ccgtatggtt actggagcat catttcacgg agtttggttt gcttggtaac
     6901 ccttatgtcg ctgctgcata tttacttggc gcgactaaaa aattgaatgt aggaactgcc
     6961 gctattgttc ttcccacagc ccatccagta cgccaacttg aagatgtgaa tttattggat
     7021 caaatgtcaa aaggacgatt tcggtttggt atttgccgag ggctttacaa caaggacttt
     7081 cgcgtattcg gcacagatat gaataacagt cgcgccttag cggaatgctg gtacgggctg
     7141 ataaagaatg gcgtgacaga gggatatatg gaagctgata atgaacatat caagttccat
     7201 aaggtaaaag taaaccccgc ggcgtatagc agaggtggcg caccggttta tgtggtggct
     7261 gaatcagctt cgacgactga gtgggctgct caatttggcc taccgatgat attaagttgg
     7321 attataaata ctaacgaaaa gaaagcacaa cttgagcttt ataatgaagt ggctcaagaa
     7381 tatgggcacg atattcataa tatcgaccat tgcttatcat atataacatc tgtagatcat
     7441 gactcaatta aagcgaaaga gatttgccgg aaatttctgg ggcattggta tgattcttat
     7501 gtgaatgcta cgactatttt tgatgattca gaccaaacaa gaggttatga tttcaataaa
     7561 gggcagtggc gtgactttgt attaaaagga cataaagata ctaatcgccg tattgattac
     7621 agttacgaaa tcaatcccgt gggaacgccg caggaatgta ttgacataat tcaaaaagac
     7681 attgatgcta caggaatatc aaatatttgt tgtggatttg aagctaatgg aacagtagac
     7741 gaaattattg cttccatgaa gctcttccag tctgatgtca tgccatttct taaagaaaaa
     7801 caacgttcgc tattatatta gctaaggaga aagaaatgaa atttggattg ttcttcctta
     7861 acttcatcaa ttcaacaact gttcaagaac aaagtatagt tcgcatgcag gaaataacgg
     7921 agtatgttga taagttgaat tttgaacaga ttttagtgta tgaaaatcat ttttcagata
     7981 atggtgttgt cggcgctcct ctgactgttt ctggttttct gctcggttta acagagaaaa
     8041 ttaaaattgg ttcattaaat cacatcatta caactcatca tcctgtccgc atagcggagg
     8101 aagcttgctt attggatcag ttaagtgaag ggagatttat tttagggttt agtgattgcg
//
